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  1. Article ; Online: Observation of the hypnotic effect of chloral hydrate solution in infantile surgery under local anesthesia.

    Jiang, Rong / Wen, Xiao-Yun / Huang, Jian-Wei / Wang, Bing

    Asian journal of surgery

    2022  Volume 46, Issue 4, Page(s) 1676–1677

    MeSH term(s) Humans ; Hypnotics and Sedatives/pharmacology ; Chloral Hydrate ; Anesthesia, Local
    Chemical Substances Hypnotics and Sedatives ; Chloral Hydrate (418M5916WG)
    Language English
    Publishing date 2022-11-01
    Publishing country China
    Document type Letter
    ZDB-ID 1068461-x
    ISSN 0219-3108 ; 1015-9584
    ISSN (online) 0219-3108
    ISSN 1015-9584
    DOI 10.1016/j.asjsur.2022.09.119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Rapid Cobas Liat SARS-CoV-2 Assay in Comparison with the Laboratory-Developed Real-Time RT-PCR Test.

    Er, Tze-Kiong / Chou, Yung-Che / Chen, Si-Yu / Huang, Jian-Wei

    Clinical laboratory

    2021  Volume 67, Issue 11

    Abstract: Background: The objective of this study was to compare the validity of two different assays for the detection of SARS-CoV-2.: Methods: We collected 50 nasopharyngeal swabs in universal transport medium from the emergency department of Asia University ...

    Abstract Background: The objective of this study was to compare the validity of two different assays for the detection of SARS-CoV-2.
    Methods: We collected 50 nasopharyngeal swabs in universal transport medium from the emergency department of Asia University Hospital for the detection of SARS-CoV-2 using reverse transcription-polymerase chain reaction (RT-PCR). The samples for the Liat SARS-CoV-2 influenza A/B test were stored at -70℃ after SARS-CoV-2 testing using the RT-PCR in order to assess method comparison.
    Results: In this study, the Limit of detection (LOD) of the cobas Liat SARS-CoV-2 and influenza A/B nucleic acid test is 12 copies/µL and the assay obtained 100% positive agreement and negative percent agreement with RT-PCR.
    Conclusions: In summary, a prefect agreement exists between the detection of SARS-CoV-2 conducted with the cobas Liat SARS-CoV-2 and influenza A/B nucleic acid test and the RT-PCR. The cobas Liat SARS-CoV-2 and influenza A/B nucleic acid test is a reliable method for the detection of SARS-CoV-2, and it only requires 20 minutes to obtain the results. On the other hand, the cobas Liat SARS-CoV-2 and influenza A/B nucleic acid test is accurate, easy to use, and provides a faster turnaround time than testing performed in the high-throughput platform.
    MeSH term(s) COVID-19 ; COVID-19 Testing ; Humans ; Laboratories ; Nasopharynx ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2 ; Sensitivity and Specificity
    Language English
    Publishing date 2021-11-10
    Publishing country Germany
    Document type Letter
    ZDB-ID 1307629-2
    ISSN 1433-6510 ; 0941-2131
    ISSN 1433-6510 ; 0941-2131
    DOI 10.7754/Clin.Lab.2021.210316
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  3. Article: Comparison of Manual and Automated Nucleic Acid Extraction Methods in Virus Transport Medium.

    Chen, Si-Yu / Chou, Yung-Che / Huang, Jian-Wei / Er, Tze-Kiong

    Clinical laboratory

    2021  Volume 67, Issue 12

    Abstract: Background: The implementation of an automated nucleic acid extraction system has many advantages over the manual methods. The purpose of this study was to evaluate the validity of two different methods for nucleic acid extraction in virus transport ... ...

    Abstract Background: The implementation of an automated nucleic acid extraction system has many advantages over the manual methods. The purpose of this study was to evaluate the validity of two different methods for nucleic acid extraction in virus transport medium.
    Methods: We collected 20 nasopharyngeal swabs in viral transport medium from the emergency department of the Asia University Hospital for the detection of SARS-CoV-2. The performance of the MaelstromTM 8 (Taiwan Advanced Nanotech) and the QIAamp Viral RNA Mini Kit (Qiagen) were compared for the extraction of nucleic acid from viral transport medium. The extracts were used for the validation of the RNA extraction procedures. The RNase P target was amplified in a one-step reverse transcription-quantitative PCR (RT-qPCR) reaction, as internal control for the extraction method.
    Results: In this study, the agreement between the two methods was good and Pearson's correlation coefficient (r) was 0.919 (p < 0.001). The mean cycle threshold value of the two methods was 29.1.
    Conclusions: Overall, the performance values of the MaelstromTM 8 and the QIAamp Viral RNA Mini Kit were comparable to each other. In summary, the MaelstromTM 8 provides a standardized procedure, avoidance of sample-to-sample cross contaminations, is easy to use, improves turnaround time and requires less hands-on time as compared to the manual extraction method. The MaelstromTM 8 is more suitable for clinical laboratories that carry small or medium-sized samples for nucleic acid extraction.
    MeSH term(s) COVID-19 ; Humans ; Laboratories, Clinical ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2 ; Sensitivity and Specificity
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-11-01
    Publishing country Germany
    Document type Letter
    ZDB-ID 1307629-2
    ISSN 1433-6510 ; 0941-2131
    ISSN 1433-6510 ; 0941-2131
    DOI 10.7754/Clin.Lab.2021.210443
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Change in the Oligomeric State of α-Synuclein Variants in Living Cells.

    Fan, Hsiu-Fang / Chen, Wen-Ling / Chen, Yan-Zhow / Huang, Jian-Wei / Shen, Yu-Xin

    ACS chemical neuroscience

    2022  Volume 13, Issue 8, Page(s) 1143–1164

    Abstract: The accumulation of β-sheet-rich α-synuclein (α-Syn) protein in human brain cells is a pathological hallmark of Parkinson's disease (PD). Moreover, it has been reported that familial PD mutations (A30P, E46K, H50Q, G51D, and A53T) accumulate at an ... ...

    Abstract The accumulation of β-sheet-rich α-synuclein (α-Syn) protein in human brain cells is a pathological hallmark of Parkinson's disease (PD). Moreover, it has been reported that familial PD mutations (A30P, E46K, H50Q, G51D, and A53T) accumulate at an accelerated rate both in vivo and in vitro. In addition, accumulations of various C-terminal α-Syn truncations, such as C-terminal-truncated N103 α-synuclein (N103), were found in an aggregated form in the brain tissue of PD patients. Fluorescent protein-tagged wild-type α-Syn, A30P, E46K, H50Q, G51D, A53T, and N103 were transfected into HEK293T and SHSY5Y cells, and their diffusion behaviors were investigated with a custom-built fluorescence microscope system. Based on our experimental results, the oligomerization of α-Syn is a time-dependent process in both HEK293T and SHSY5Y cells, and the oligomer state approaches a plateau after 48 h of transfection. The change in the oligomeric state of E46K, H50Q, and G51D exhibited a similar trend to the wild type at a lower concentration but became intense at a higher concentration. A53T and N103 possess smaller diffusion coefficients than wild-type α-synuclein and other family PD mutations, indicating that these two mutants could form higher oligomeric states or stronger interactions in HEK293T and SHSY5Y cells. In contrast, the smallest oligomer and the lowest intracellular interaction among all investigated α-Syn variants were found for A30P. These phenomena indicated the presence of different pathogeneses among familial PD mutants and C-terminal α-Syn truncations.
    MeSH term(s) HEK293 Cells ; Humans ; Mutation/genetics ; Parkinson Disease/metabolism ; alpha-Synuclein/genetics ; alpha-Synuclein/metabolism
    Chemical Substances alpha-Synuclein
    Language English
    Publishing date 2022-04-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1948-7193
    ISSN (online) 1948-7193
    DOI 10.1021/acschemneuro.1c00646
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Comparison of laparoscopic common bile duct exploration plus cholecystectomy and endoscopic retrograde cholangiopancreatography followed by laparoscopic cholecystectomy for elderly patients with common bile duct stones and gallbladder stones.

    Wu, Po-Hsuan / Yu, Min-Wei / Chuang, Shih-Chang / Wang, Shen-Nien / Kuo, Kung-Kai / Chang, Wen-Tsan / Chuang, Shu-Hung / Su, Wen-Lung / Huang, Jian-Wei / Chen, Ling-An

    Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract

    2024  

    Abstract: Background: Common bile duct (CBD) stones commonly occur in cholecystectomy cases. The management options include laparoscopic CBD exploration (LCBDE) or endoscopic retrograde cholangiopancreatography (ERCP) followed by laparoscopic cholecystectomy (LC). ...

    Abstract Background: Common bile duct (CBD) stones commonly occur in cholecystectomy cases. The management options include laparoscopic CBD exploration (LCBDE) or endoscopic retrograde cholangiopancreatography (ERCP) followed by laparoscopic cholecystectomy (LC). Although ERCP is fully developed, it has complications, and LCBDE is a proven alternative. This study aimed to evaluate the safety and efficacy of these treatments in elderly individuals aged ≥70 years.
    Methods: A retrospective study between January 2015 and July 2022 included 160 elderly patients (aged ≥70 years) diagnosed with cholelithiasis and choledocholithiasis. The patients were divided into 1-stage (LCBDE [n = 80]) or 2-stage (ERCP followed by LC [n = 80]) treatment groups. Data collected encompassed comorbidities, symptoms, bile duct clearance, postoperative complications, and long-term outcomes for systematic analysis.
    Results: This study analyzed 160 patients treated for CBD stones, comparing 1-stage and 2-stage groups. The 1-stage group had more female patients than the 2-stage group (57.5% vs 37.5%, respectively). The 1-stage group had a mean age of 80.55 ± 7.00 years, which was higher than the mean age in the 2-stage group. American Society of Anesthesiologists classification, Charlson Comorbidity Index, and laboratory findings were similar. Pancreatitis and cholangitis occurred after ERCP in the 2-stage group. Stone clearance rates (92.35% [1-stage group] vs 95.00% [2-stage group]) and biliary leakage incidence (7.5% [1-stage group] vs 3.0% [2-stage group]) were similar, as were postoperative complications and long-term recurrence rates (13.0% [1-stage group] vs 12.5% [2-stage group]).
    Conclusion: Our research indicates that both the combination of LCBDE and LC and the sequence of ERCP followed by LC are equally efficient and secure when treating CBD stones in elderly patients. Consequently, the 1-stage procedure may be considered the preferred treatment approach for this demographic.
    Language English
    Publishing date 2024-03-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2012365-6
    ISSN 1873-4626 ; 1934-3213 ; 1091-255X
    ISSN (online) 1873-4626 ; 1934-3213
    ISSN 1091-255X
    DOI 10.1016/j.gassur.2024.02.026
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  6. Article ; Online: Evaluation of the association between vitamin D and lung cancer skin metastasis: A protocol for systematic review.

    Zhao, Dan / Wang, Tao / Li, Yu-Feng / Huang, Jian-Wei

    Medicine

    2020  Volume 99, Issue 49, Page(s) e23281

    Abstract: Background: This study aims to investigate the association between vitamin D (VD) and lung cancer skin metastasis (LCSM).: Methods: The following databases will be retrieved from the beginning to the present of each database without language ... ...

    Abstract Background: This study aims to investigate the association between vitamin D (VD) and lung cancer skin metastasis (LCSM).
    Methods: The following databases will be retrieved from the beginning to the present of each database without language limitation: PUBMED, EMBASE, Cochrane Library, Web of Science, CBM, and CNKI. The reference lists of included trials and other sources will also be checked. Two researchers will independently undertake literature selection, data collection, and study quality evaluation. We will utilize a fixed or random-effect model to pool the data according to the heterogeneity test. The RevMan 5.3 software will be used to analyze the data and perform meta-analysis.
    Results: This study will summarize high quality study to explore the association between VD and LCSM.
    Conclusion: The findings of this study will help to judge whether there is association between VD and LCSM.
    Ethics and dissemination: No research ethical approval is required in this study, because it will only analyze published data. It is expected to disseminate through a peer-reviewed journal.
    Study registration: osf.io/ph2au.
    MeSH term(s) Humans ; Lung Neoplasms/blood ; Lung Neoplasms/pathology ; Neoplasm Metastasis ; Skin/pathology ; Skin Neoplasms/blood ; Skin Neoplasms/secondary ; Systematic Reviews as Topic ; Vitamin D/blood
    Chemical Substances Vitamin D (1406-16-2)
    Language English
    Publishing date 2020-12-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80184-7
    ISSN 1536-5964 ; 0025-7974
    ISSN (online) 1536-5964
    ISSN 0025-7974
    DOI 10.1097/MD.0000000000023281
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  7. Article ; Online: Alternative Laparoscopic Intracorporeal Pringle Maneuver by Huang's Loop.

    Huang, Jian-Wei / Su, Wen-Lung / Wang, Shen-Nien

    World journal of surgery

    2018  Volume 42, Issue 10, Page(s) 3312–3315

    Abstract: Aim: This paper aims to describe an intracorporeal tourniquet method for laparoscopic Pringle maneuver (PM).: Methods: One shortened Foley tube with side-hole on the tip was put into the abdomen. Then, the tail was pulled out through the side-hole to ...

    Abstract Aim: This paper aims to describe an intracorporeal tourniquet method for laparoscopic Pringle maneuver (PM).
    Methods: One shortened Foley tube with side-hole on the tip was put into the abdomen. Then, the tail was pulled out through the side-hole to make a loop to encircle porta hepatis for inflow control.
    Result: It is easy to keep the tension by a metallic clip, and when released, the clip can be removed and the loop loosened.
    Conclusion: Therefore, PM could be performed inside the abdomen without special instrument nor extra trocar port. The intracorporeal Pringle maneuver with Huang's loop could be routinely used during laparoscopic liver resection even for a laparoscopic beginner because it is so easily learnt, safe, and effective.
    MeSH term(s) Abdominal Cavity/surgery ; Hepatectomy/instrumentation ; Hepatectomy/methods ; Humans ; Laparoscopy/methods ; Liver/surgery
    Language English
    Publishing date 2018-03-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 224043-9
    ISSN 1432-2323 ; 0364-2313
    ISSN (online) 1432-2323
    ISSN 0364-2313
    DOI 10.1007/s00268-018-4584-z
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  8. Article ; Online: Exploring the Impact of Glyoxal Glycation on β-Amyloid Peptide (Aβ) Aggregation in Alzheimer's Disease.

    Hu, Kai-Wei / Fan, Hsiu-Fang / Lin, Han-Chen / Huang, Jian-Wei / Chen, Yu-Chieh / Shen, Cai-Ling / Shih, Yao-Hsiang / Tu, Ling-Hsien

    The journal of physical chemistry. B

    2021  Volume 125, Issue 21, Page(s) 5559–5571

    Abstract: Alzheimer's disease (AD) is characterized by the presence of extracellular senile plaques formed by β-amyloid (Aβ) peptides in the patient's brain. Previous studies have shown that the plaques in the AD brains are colocalized with the advanced glycation ... ...

    Abstract Alzheimer's disease (AD) is characterized by the presence of extracellular senile plaques formed by β-amyloid (Aβ) peptides in the patient's brain. Previous studies have shown that the plaques in the AD brains are colocalized with the advanced glycation end products, which is mainly formed from a series of nonenzymatic reactions of proteins with reducing sugars or reactive dicarbonyls. Glycation was also demonstrated to increase the neurotoxicity of the Aβ peptides. To clarify the impact of glycation on Aβ aggregation, we synthesized two glycated Aβ42 peptides by replacing Lys16 and Lys28 with N
    MeSH term(s) Alzheimer Disease ; Amyloid beta-Peptides/metabolism ; Brain ; Glycosylation ; Glyoxal ; Humans ; Peptide Fragments/metabolism
    Chemical Substances Amyloid beta-Peptides ; Peptide Fragments ; Glyoxal (50NP6JJ975)
    Language English
    Publishing date 2021-05-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1520-5207
    ISSN (online) 1520-5207
    DOI 10.1021/acs.jpcb.1c02797
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: The association between oral health status and the clinical outcome of cirrhotic patients on the waiting list for liver transplantation.

    Wu, Ju-Hui / Lee, Chen-Yi / Chang, Wen-Tsan / Wu, Po-Hsuan / Chen, Lin-Ann / Huang, Jian-Wei / Su, Wen-Lung / Kuo, Kung-Kai

    The Kaohsiung journal of medical sciences

    2021  Volume 37, Issue 10, Page(s) 910–917

    Abstract: Liver transplantation (LT) candidates often present with poor oral hygiene, which could potentially lead to systemic infections and sepsis owing to their cirrhotic state. In this study, we investigated the oral health status of LT candidates and propose ... ...

    Abstract Liver transplantation (LT) candidates often present with poor oral hygiene, which could potentially lead to systemic infections and sepsis owing to their cirrhotic state. In this study, we investigated the oral health status of LT candidates and propose guidance for the detection and treatment of encountered oral lesions among these patients. The decayed, missing, and filled teeth (DMFT) index was determined through oral examination. The presence of dental calculus was detected using panoramic radiography and defined by the radiopaque dental calculus (RDC). From January 2011 to August 2018, 56 LT candidates were enrolled with a median follow-up of 39 months. The overall mean numbers of decayed, missing, and filled teeth among these patients were 2.7 ± 2.8, 10.9 ± 8.3, and 5.4 ± 4.5, respectively. Eighteen patients (32.1%) had RDC. The 5-year survival rates of all 56 patients was 57.7%, while that of those who either received LT (23 patients) or not were 82.1% and 39.8%, respectively. A Cox regression model revealed better overall survival of patients after LT (adjusted hazard ratio [aHR] = 0.067, p = 0.001), worse survival among patients with RDC (aHR = 3.468, p = 0.010), at Child-Pugh stages B and C (aHR for stage B = 11.889, p = 0.028; aHR for stage C = 19.257, p = 0.013) compared to patients at Child-Pugh stage A, and those with a model for end-stage liver disease (MELD) score ≥25 (aHR = 13.721, p = 0.018). This study demonstrates that RDC was associated with worse prognosis in LT candidates. We therefore recommend that interprofessional collaboration should be a routine preoperative procedure for the evaluation of oral hygiene among LT candidates.
    MeSH term(s) Female ; Humans ; Liver Cirrhosis/surgery ; Liver Transplantation ; Male ; Middle Aged ; Oral Health ; Treatment Outcome ; Waiting Lists
    Language English
    Publishing date 2021-07-19
    Publishing country China (Republic : 1949- )
    Document type Journal Article
    ZDB-ID 639302-0
    ISSN 2410-8650 ; 0257-5655
    ISSN (online) 2410-8650
    ISSN 0257-5655
    DOI 10.1002/kjm2.12406
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  10. Article ; Online: Loss of Glycine

    Huang, Jian-Wei / Chen, Chao-Ju / Yen, Chia-Hung / Chen, Yi-Ming Arthur / Liu, Yu-Peng

    International journal of molecular sciences

    2019  Volume 20, Issue 17

    Abstract: Imbalance of lipid metabolism is a main cause of metabolic syndrome leading to life-threatening metabolic diseases. Angiopoietin-like protein 8 (Angptl8) was recently identified as a liver and adipose tissue-released hormone that is one of the molecules ... ...

    Abstract Imbalance of lipid metabolism is a main cause of metabolic syndrome leading to life-threatening metabolic diseases. Angiopoietin-like protein 8 (Angptl8) was recently identified as a liver and adipose tissue-released hormone that is one of the molecules involved in triglyceride metabolism. However, the regulatory mechanism of Angptl8 is largely unknown. A high fat diet (HFD)-fed mouse model, which showed high cholesterol, high triglyceride, and high insulin in the blood, revealed the upregulation of hepatic and plasma Angptl8 and the downregulation of hepatic glycine
    MeSH term(s) Angiopoietin-like Proteins/blood ; Angiopoietin-like Proteins/genetics ; Angiopoietin-like Proteins/metabolism ; Animals ; Cells, Cultured ; Diet, High-Fat/adverse effects ; Gene Expression Regulation/drug effects ; Gene Expression Regulation/genetics ; Glycine N-Methyltransferase/blood ; Glycine N-Methyltransferase/genetics ; Glycine N-Methyltransferase/metabolism ; Hepatocytes/metabolism ; Insulin/pharmacology ; Lipids/blood ; Liver/enzymology ; Liver/metabolism ; Male ; Metabolic Syndrome/etiology ; Metabolic Syndrome/genetics ; Metabolic Syndrome/metabolism ; Mice, Inbred C57BL ; Mice, Knockout ; Signal Transduction/genetics
    Chemical Substances ANGPTL8 protein, mouse ; Angiopoietin-like Proteins ; Insulin ; Lipids ; Glycine N-Methyltransferase (EC 2.1.1.20)
    Language English
    Publishing date 2019-08-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms20174223
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