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Article ; Online: The Different Faces of the TDP-43 Low-Complexity Domain: The Formation of Liquid Droplets and Amyloid Fibrils.

Chien, Hung-Ming / Lee, Chi-Chang / Huang, Joseph Jen-Tse

International journal of molecular sciences

2021 Volume 22, Issue 15

Abstract: Transactive response DNA-binding protein 43 (TDP-43) is a nucleic acid-binding protein that is involved in transcription and translation regulation, non-coding RNA processing, and stress granule assembly. Aside from its multiple functions, it is also ... ...

Abstract	Transactive response DNA-binding protein 43 (TDP-43) is a nucleic acid-binding protein that is involved in transcription and translation regulation, non-coding RNA processing, and stress granule assembly. Aside from its multiple functions, it is also known as the signature protein in the hallmark inclusions of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD) patients. TDP-43 is built of four domains, but its low-complexity domain (LCD) has become an intense research focus that brings to light its possible role in TDP-43 functions and involvement in the pathogenesis of these neurodegenerative diseases. Recent endeavors have further uncovered the distinct biophysical properties of TDP-43 under various circumstances. In this review, we summarize the multiple structural and biochemical properties of LCD in either promoting the liquid droplets or inducing fibrillar aggregates. We also revisit the roles of the LCD in paraspeckles, stress granules, and cytoplasmic inclusions to date.
MeSH term(s)	Amyloid/metabolism ; Animals ; Cytoplasmic Granules/metabolism ; DNA-Binding Proteins/chemistry ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Humans ; Lipid Droplets/metabolism ; Protein Domains ; TDP-43 Proteinopathies/metabolism
Chemical Substances	Amyloid ; DNA-Binding Proteins ; TARDBP protein, human
Language	English
Publishing date	2021-07-30
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22158213
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Article ; Online: Comparison of early surgical outcomes of robotic and laparoscopic colorectal cancer resection reported by a busy district general hospital in England.

Butnari, Valentin / Sultana, Momotaz / Mansuri, Ahmer / Rao, Christopher / Kaul, Sandeep / Boulton, Richard / Huang, Joseph / Rajendran, Nirooshun

Scientific reports

2024 Volume 14, Issue 1, Page(s) 9227

Abstract: Robotic platforms provide a stable tool with high-definition views and improved ergonomics compared to laparoscopic approaches. The aim of this retrospective study was to compare the intra- and short-term postoperative results of oncological resections ... ...

Abstract	Robotic platforms provide a stable tool with high-definition views and improved ergonomics compared to laparoscopic approaches. The aim of this retrospective study was to compare the intra- and short-term postoperative results of oncological resections performed robotically (RCR) and laparoscopically (LCR) at a single centre. Between February 2020 and October 2022, retrospective data on RCR were compared to LCR undertaken during the same period. Parameters compared include total operative time, length of stay (LOS), re-admission rates, 30-day morbidity. 100 RCR and 112 LCR satisfied inclusion criteria. There was no difference between the two group's demographic and tumour characteristics. Overall, median operative time was shorter in LCR group [200 vs. 247.5 min, p < 0.005], but this advantage was not observed with pelvic and muti-quadrant resections. There was no difference in the rate of conversion [5(5%) vs. 5(4.5%), p > 0.95]. With respect to perioperative outcomes, there was no difference in the overall morbidity, or mortality between RCR and LCR, in particular requirement for blood transfusion [3(3%) vs. 5(4.5%), p 0.72], prolonged ileus [9(9%) vs. 15(13.2%), p 0.38], surgical site infections [5(4%) vs. 5(4.4%), p > 0.95], anastomotic leak [7(7%) vs. 5(4.4%), p 0.55], and re-operation rate [9(9%) vs. 7(6.3%), p 0.6]. RCR had shorter LOS by one night, but this did not reach statistical significance. No difference was observed in completeness of resection but there was a statically significant increase in lymph node harvest in the robotic series. Robotic approach to oncological colorectal resections is safe, with comparable intra- and peri-operative morbidity and mortality to laparoscopic surgery.
MeSH term(s)	Humans ; Laparoscopy/methods ; Robotic Surgical Procedures/methods ; Robotic Surgical Procedures/adverse effects ; Male ; Female ; Colorectal Neoplasms/surgery ; Colorectal Neoplasms/mortality ; Aged ; England/epidemiology ; Middle Aged ; Retrospective Studies ; Length of Stay ; Operative Time ; Treatment Outcome ; Hospitals, General ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Aged, 80 and over ; Hospitals, District ; Adult
Language	English
Publishing date	2024-04-22
Publishing country	England
Document type	Journal Article ; Comparative Study
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-57110-1
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Article ; Online: Laparoscopic right hemicolectomy with complete mesocolic excision and D3 lymphadenectomy using the open book approach: a video vignette.

Butnari, Valentin / Mansuri, Ahmer / Momotaz, Sultana / Osilli, Dixon / Boulton, Richard / Huang, Joseph / Rajendran, Nirooshun / Kaul, Sandeep

Journal of minimally invasive surgery

2024 Volume 27, Issue 1, Page(s) 47–50

Abstract: According to the concept of total mesorectal excision for rectal cancer, Hohenberger translated this concept to colonic cancer by introducing complete mesocolic excision (CME). The concept of this surgical technique was further elucidated by Benz et al. ... ...

Abstract	According to the concept of total mesorectal excision for rectal cancer, Hohenberger translated this concept to colonic cancer by introducing complete mesocolic excision (CME). The concept of this surgical technique was further elucidated by Benz et al. in the form of an open book approach. This article presents and demonstrates in a video a case of laparoscopic right hemicolectomy with CME and D3 lymphadenectomy using open book approach in the treatment of a T3N1M0 distal ascending colonic adenocarcinoma. The final pathology report confirmed moderately differentiated adenocarcinoma with a maximum tumor size of 55 mm and 0/60 lymph nodes. The mesocolic fascia was intact and R0 was achieved. The final staging was pT3pN0pM0. However, D3 lymphadenectomy is not universally adopted due to concerns of higher morbidity we believe that with adequate training and supervision CME with D3 LDN is feasible and safe to be offered to all right-sided colorectal cancers with curative intent treatment.
Language	English
Publishing date	2024-03-16
Publishing country	Korea (South)
Document type	Journal Article
ISSN	2234-5248
ISSN (online)	2234-5248
DOI	10.7602/jmis.2024.27.1.47
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Article ; Online: Learning from the TDP-43 amyloidogenic sequences in neurodegenerative diseases.

Lee, Chi-Chang / He, Ruei-Yu / Huang, Joseph Jen-Tse

Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis

2019 Volume 26, Issue sup1, Page(s) 154–155

MeSH term(s)	Amino Acid Sequence ; Amyloidogenic Proteins/chemistry ; Amyloidogenic Proteins/genetics ; Amyloidogenic Proteins/metabolism ; DNA-Binding Proteins/chemistry ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Humans ; Mutation ; Neurodegenerative Diseases/genetics ; Neurodegenerative Diseases/metabolism ; Peptides/chemistry ; Peptides/genetics ; Peptides/metabolism ; Protein Domains
Chemical Substances	Amyloidogenic Proteins ; DNA-Binding Proteins ; Peptides ; TARDBP protein, human
Language	English
Publishing date	2019-07-25
Publishing country	England
Document type	Journal Article
ZDB-ID	1205246-2
ISSN	1744-2818 ; 1350-6129
ISSN (online)	1744-2818
ISSN	1350-6129
DOI	10.1080/13506129.2019.1582513
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Zs.A 4114: Show issues

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Article ; Online: Effectiveness of calcium channel blockers for Raynaud phenomenon.

Seehusen, Dean A / Huang, Joseph

American family physician

2014 Volume 90, Issue 3, Page(s) 143–144

MeSH term(s)	Calcium Channel Blockers/adverse effects ; Calcium Channel Blockers/therapeutic use ; Humans ; Raynaud Disease/drug therapy ; Treatment Outcome
Chemical Substances	Calcium Channel Blockers
Language	English
Publishing date	2014-08-01
Publishing country	United States
Document type	Journal Article ; Meta-Analysis
ZDB-ID	412694-4
ISSN	1532-0650 ; 0002-838X ; 0572-3612
ISSN (online)	1532-0650
ISSN	0002-838X ; 0572-3612
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Article ; Online: Degradation of neurodegenerative disease-associated TDP-43 aggregates and oligomers via a proteolysis-targeting chimera.

Tseng, Yu-Ling / Lu, Po-Chao / Lee, Chi-Chang / He, Ruei-Yu / Huang, Yung-An / Tseng, Yin-Chen / Cheng, Ting-Jen Rachel / Huang, Joseph Jen-Tse / Fang, Jim-Min

Journal of biomedical science

2023 Volume 30, Issue 1, Page(s) 27

Abstract: Background: Amyotrophic lateral sclerosis (ALS) associated with TAR DNA-binding protein 43 (TDP-43) aggregation has been considered as a lethal and progressive motor neuron disease. Recent studies have shown that both C-terminal TDP-43 (C-TDP-43) ... ...

Abstract	Background: Amyotrophic lateral sclerosis (ALS) associated with TAR DNA-binding protein 43 (TDP-43) aggregation has been considered as a lethal and progressive motor neuron disease. Recent studies have shown that both C-terminal TDP-43 (C-TDP-43) aggregates and oligomers were neurotoxic and pathologic agents in ALS and frontotemporal lobar degeneration (FTLD). However, misfolding protein has long been considered as an undruggable target by applying conventional inhibitors, agonists, or antagonists. To provide this unmet medical need, we aim to degrade these misfolding proteins by designing a series of proteolysis targeting chimeras (PROTACs) against C-TDP-43. Methods: By applying filter trap assay, western blotting, and microscopy imaging, the degradation efficiency of C-TDP-43 aggregates was studied in Neuro-2a cells overexpressing eGFP-C-TDP-43 or mCherry-C-TDP-43. The cell viability was characterized by alarmarBlue assay. The beneficial and disaggregating effects of TDP-43 PROTAC were examined with the YFP-C-TDP-43 transgenic C. elegans by motility assay and confocal microscopy. The impact of TDP-43 PROTAC on C-TDP-43 oligomeric intermediates was monitored by fluorescence lifetime imaging microscopy and size exclusion chromatography in the Neuro-2a cells co-expressing eGFP-C-TDP-43 and mCherry-C-TDP-43. Results: Four PROTACs with different linker lengths were synthesized and characterized. Among these chimeras, PROTAC 2 decreased C-TDP-43 aggregates and relieved C-TDP-43-induced cytotoxicity in Neuro-2a cells without affecting endogenous TDP-43. We showed that PROTAC 2 bound to C-TDP-43 aggregates and E3 ligase to initiate ubiquitination and proteolytic degradation. By applying advanced microscopy, it was further shown that PROTAC 2 decreased the compactness and population of C-TDP-43 oligomers. In addition to cellular model, PROTAC 2 also improved the motility of transgenic C. elegans by reducing the C-TDP-43 aggregates in the nervous system. Conclusions: Our study demonstrated the dual-targeting capacity of the newly-designed PROTAC 2 against both C-TDP-43 aggregates and oligomers to reduce their neurotoxicity, which shed light on the potential drug development for ALS as well as other neurodegenerative diseases.
MeSH term(s)	Animals ; Amyotrophic Lateral Sclerosis/metabolism ; Neurodegenerative Diseases/genetics ; Proteolysis ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; DNA-Binding Proteins/metabolism ; Animals, Genetically Modified
Chemical Substances	DNA-Binding Proteins
Language	English
Publishing date	2023-04-26
Publishing country	England
Document type	Journal Article
ZDB-ID	1193378-1
ISSN	1423-0127 ; 1021-7770
ISSN (online)	1423-0127
ISSN	1021-7770
DOI	10.1186/s12929-023-00921-7
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Zs.A 4037: Show issues

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Article ; Online: Single Port Laparoscopic Total and Subtotal Colectomies for Inflammatory Bowel Disease in a District General Hospital.

Zosimas, Dimitrios / Mansouri, Ahmer / Lykoudis, Panagis M / Wain, Mehmood / Huang, Joseph

Journal of laparoendoscopic & advanced surgical techniques. Part A

2019 Volume 29, Issue 11, Page(s) 1431–1435

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Colectomy/adverse effects ; Colectomy/methods ; Colitis, Ulcerative/surgery ; Conversion to Open Surgery ; Female ; Follow-Up Studies ; Hernia, Abdominal/etiology ; Hospitals, District ; Hospitals, General ; Humans ; Laparoscopy/adverse effects ; Laparoscopy/methods ; Length of Stay ; Male ; Middle Aged ; Operative Time ; Postoperative Complications/etiology ; Retrospective Studies ; Treatment Outcome ; United Kingdom ; Young Adult
Language	English
Publishing date	2019-09-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	1381909-4
ISSN	1557-9034 ; 1092-6429
ISSN (online)	1557-9034
ISSN	1092-6429
DOI	10.1089/lap.2019.0259
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Article ; Online: Biocompatible Inhibitor Based on Chitosan and Amphiphilic Peptide against Mutant Huntingtin Toxicity.

Wahyuningtyas, Devi / Chen, Wen-Hao / Huang, Chen-Han / He, Yu-Jung / Huang, Joseph Jen-Tse

Chembiochem : a European journal of chemical biology

2019 Volume 20, Issue 16, Page(s) 2133–2140

Abstract: Huntington's disease (HD) is classified as a protein-misfolding disease correlated with the mutant Huntingtin (mHtt) protein with abnormally expanded polyglutamine (polyQ) domains. Because no effective drugs have yet been reported, attempts to develop ... ...

Abstract	Huntington's disease (HD) is classified as a protein-misfolding disease correlated with the mutant Huntingtin (mHtt) protein with abnormally expanded polyglutamine (polyQ) domains. Because no effective drugs have yet been reported, attempts to develop better therapy to delay the age of onset are in urgent demand. In this study, an amphiphilic peptide consisting of negatively charged hexaglutamic acid and a stretch of decaglutamine (E
MeSH term(s)	Biocompatible Materials/chemical synthesis ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Chitosan/chemistry ; Chitosan/pharmacology ; Humans ; Huntingtin Protein/antagonists & inhibitors ; Huntingtin Protein/genetics ; Huntingtin Protein/metabolism ; Huntington Disease/drug therapy ; Huntington Disease/genetics ; Huntington Disease/metabolism ; Molecular Structure ; Mutation ; Particle Size ; Peptides/chemical synthesis ; Peptides/chemistry ; Peptides/pharmacology ; Protein Aggregates/drug effects ; Surface Properties ; Surface-Active Agents/chemical synthesis ; Surface-Active Agents/chemistry ; Surface-Active Agents/pharmacology
Chemical Substances	Biocompatible Materials ; HTT protein, human ; Huntingtin Protein ; Peptides ; Protein Aggregates ; Surface-Active Agents ; Chitosan (9012-76-4)
Language	English
Publishing date	2019-07-11
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020469-3
ISSN	1439-7633 ; 1439-4227
ISSN (online)	1439-7633
ISSN	1439-4227
DOI	10.1002/cbic.201900242
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Article ; Online: A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors

Uhde-Stone Claudia / Huang Joseph / Lu Biao

Biological Procedures Online, Vol 14, Iss 1, p

2012 Volume 8

Abstract: Abstract Background Transcription activator-like effectors (TALEs) are a class of naturally occurring transcription effectors that recognize specific DNA sequences and modulate gene expression. The modularity of TALEs DNA binding domain enables sequence- ... ...

Abstract	Abstract Background Transcription activator-like effectors (TALEs) are a class of naturally occurring transcription effectors that recognize specific DNA sequences and modulate gene expression. The modularity of TALEs DNA binding domain enables sequence-specific perturbation and offers broad applications in genetic and epigenetic studies. Although the efficient construction of TALEs has been established, robust functional tools to assess their functions remain lacking. Results We established a dual reporter system that was specifically designed for real-time monitoring and quantifying gene expression mediated by TALEs. We validated both sensitivity and specificity of this dual-reporter system in mammalian cells, and demonstrated that this dual reporter system is robust and potentially amenable to high throughput (HTP) applications. Conclusion We have designed, constructed and validated a novel dual reporter system for assessing TALE mediated gene regulations. This system offers a robust and easy-to- use tool for real-time monitoring and quantifying gene expression in mammalian cells.
Keywords	Dual reporter ; Gene editing ; Transcription activator-like effector ; Green fluorescent protein ; Firefly luciferase ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	612
Language	English
Publishing date	2012-08-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Painful, swollen, oozing right great toe.

Huang, Joseph C / Noss, Matthew R / DeMichiei, Mark E

The Journal of family practice

2016 Volume 65, Issue 5, Page(s) E5–E8

Abstract: We initially suspected septic arthritis or osteomyelitis in this patient. Further investigation proved it was neither. ...

Abstract	We initially suspected septic arthritis or osteomyelitis in this patient. Further investigation proved it was neither.
Language	English
Publishing date	2016-05
Publishing country	United States
Document type	Case Reports
ZDB-ID	197883-4
ISSN	1533-7294 ; 0094-3509
ISSN (online)	1533-7294
ISSN	0094-3509
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Zs.A 1274: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 444: Show issues

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