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  1. Article ; Online: Correction: Fang et al. In Vivo Rodent Models of Type 2 Diabetes and Their Usefulness for Evaluating Flavonoid Bioactivity.

    Fang, Jia-You / Lin, Chih-Hung / Huang, Tse-Hung / Chuang, Shih-Yi

    Nutrients

    2023  Volume 15, Issue 13

    Abstract: ... Missing ... ...

    Abstract Missing Citation
    Language English
    Publishing date 2023-06-26
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu15132881
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Antrodia cinnamomea extract alleviates bleomycin-induced pulmonary fibrosis in mice by inhibiting the mTOR pathway.

    Lan, Ying-Wei / Chen, Chia-En / Huang, Tsung-Teng / Huang, Tse-Hung / Chen, Chuan-Mu / Chong, Kowit-Yu

    Biomedical journal

    2024  , Page(s) 100720

    Abstract: Background: Pulmonary fibrosis is a progressive diffuse parenchymal lung disorder with a high mortality rate. Studies have indicated that injured lung tissues release various pro-inflammatory factors, and produce a large amount of nitric oxide. There is ...

    Abstract Background: Pulmonary fibrosis is a progressive diffuse parenchymal lung disorder with a high mortality rate. Studies have indicated that injured lung tissues release various pro-inflammatory factors, and produce a large amount of nitric oxide. There is also accumulation of collagen and oxidative stress-induced injury, collectively leading to pulmonary fibrosis. Antrodia cinnamomea is an endemic fungal growth in Taiwan, and its fermented extracts exert anti-inflammatory effects to alleviate liver damages. Hence, we hypothesized and tested the feasibility of using A. cinnamomea extracts for treatment of pulmonary fibrosis.
    Methods: The TGF-β1-induced human lung fibroblast cells (MRC-5) in vitro cell assay were used to evaluate the effects of A. cinnamomea extracts on the collagen production in MRC-5. Eight-week-old ICR mice were intratracheally administered bleomycin and then fed with an A. cinnamomea extract on day 3 post-administration of bleomycin. At day 21 post-bleomycin administration, the pulmonary functional test, the expression level of inflammation- and fibrosis-related genes in the lung tissue, and the histopathological change were examined.
    Results: The A. cinnamomea extract significantly attenuated the expression level of collagen in the TGF-β1-induced MRC-5 cells. In the A. cinnamome-treated bleomycin-induced lung fibrotic mice, the bodyweight increased, pulmonary functions improved, the lung tissues expression level of inflammatory factor and the fibrotic indicator were decreased, and the histopathological results showed the reduction of thickening of the inter-alveolar septa.
    Conclusions: The Antrodia cinnamomea extract significant protects mice against bleomycin-induced lung injuries through improvement of body weight gain and lung functions, and attenuation of expression of inflammatory and fibrotic indicators.
    Language English
    Publishing date 2024-04-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2698541-X
    ISSN 2320-2890 ; 2320-2890
    ISSN (online) 2320-2890
    ISSN 2320-2890
    DOI 10.1016/j.bj.2024.100720
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  3. Article ; Online: Small interfering RNA-based nanotherapeutics for treating skin-related diseases.

    Chang, Yen-Tzu / Huang, Tse-Hung / Alalaiwe, Ahmed / Hwang, Erica / Fang, Jia-You

    Expert opinion on drug delivery

    2023  Volume 20, Issue 6, Page(s) 757–772

    Abstract: Introduction: RNA interference (RNAi) has demonstrated great potential in treating skin-related diseases, as small interfering RNA (siRNA) can efficiently silence specific genes. The design of skin delivery systems for siRNA is important to protect the ... ...

    Abstract Introduction: RNA interference (RNAi) has demonstrated great potential in treating skin-related diseases, as small interfering RNA (siRNA) can efficiently silence specific genes. The design of skin delivery systems for siRNA is important to protect the nucleic acid while facilitating both skin targeting and cellular ingestion. Entrapment of siRNA into nanocarriers can accomplish these aims, contributing to improved targeting, controlled release, and increased transfection.
    Areas covered: The siRNA-based nanotherapeutics for treating skin disorders are summarized. First, the mechanisms of RNAi are presented, followed by the introduction of challenges for skin therapy. Then, the different nanoparticle types used for siRNA skin delivery are described. Subsequently, we introduce the mechanisms of how nanoparticles enhance siRNA skin penetration. Finally, the current investigations associated with nanoparticulate siRNA application in skin disease management are reviewed.
    Expert opinion: The potential application of nanotherapeutic RNAi allows for a novel skin application strategy. Further clinical studies are required to confirm the findings in the cell-based or animal experiments. The capability of large-scale production and reproducibility of nanoparticle products are also critical for translation to commercialization. siRNA delivery by nanocarriers should be optimized to attain cutaneous targeting without the risk of toxicity.
    MeSH term(s) Animals ; RNA, Small Interfering ; Reproducibility of Results ; RNA Interference ; Skin Diseases/drug therapy ; Transfection ; Nanoparticles
    Chemical Substances RNA, Small Interfering
    Language English
    Publishing date 2023-04-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2167286-6
    ISSN 1744-7593 ; 1742-5247
    ISSN (online) 1744-7593
    ISSN 1742-5247
    DOI 10.1080/17425247.2023.2206646
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  4. Article ; Online: Protective effects of myricetin on airway inflammation and oxidative stress in ovalbumin-induced asthma mice.

    Huang, Wen-Chung / Wu, Shu-Ju / Yeh, Kuo-Wei / Huang, Tse-Hung / Liou, Chian-Jiun

    The Journal of nutritional biochemistry

    2023  Volume 123, Page(s) 109485

    Abstract: Myricetin, a flavonoid isolated from many edible vegetables and fruits, has multiple biological effects, including anti-inflammatory and anti-tumor effects. Myricetin could inhibit mast cell degranulation in vitro, and it reduced the eosinophil content ... ...

    Abstract Myricetin, a flavonoid isolated from many edible vegetables and fruits, has multiple biological effects, including anti-inflammatory and anti-tumor effects. Myricetin could inhibit mast cell degranulation in vitro, and it reduced the eosinophil content in bronchoalveolar lavage fluid (BALF) of ovalbumin (OVA)-sensitized mice. However, it remains unclear whether myricetin alleviates airway hyperresponsiveness (AHR), airway inflammation, and oxidative stress in asthma. Here, we investigated whether myricetin attenuated AHR, airway inflammation, and eosinophil infiltration in lungs of asthmatic mice. Mice were sensitized with OVA, then injected intraperitoneally with myricetin to investigate anti-inflammatory and antioxidant effects of myricetin. Moreover, we examined its effects on human bronchial epithelial BEAS-2B cells stimulated with TNF-α and IL-4, in vitro. Myricetin effectively mitigated eosinophil infiltration, AHR, and goblet cell hyperplasia in lung, and it reduced Th2 cytokine expression in BALF from asthmatic mice. Myricetin effectively promoted glutathione and superoxide dismutase productions and mitigated malondialdehyde expressions in mice by promoting Nrf2/HO-1 expression. Myricetin also reduced the production of proinflammatory cytokines, eotaxins, and reactive oxygen species in BEAS-2B cells. Myricetin effectively suppressed ICAM-1 expression in inflammatory BEAS-2B cells, which suppressed monocyte cell adherence. These results suggested that myricetin could effectively improve asthma symptoms, mainly through blocking Th2-cell activation, which reduced oxidative stress, AHR, and airway inflammation.
    MeSH term(s) Humans ; Animals ; Mice ; Ovalbumin/toxicity ; Asthma/chemically induced ; Asthma/drug therapy ; Asthma/pathology ; Lung ; Flavonoids/pharmacology ; Flavonoids/therapeutic use ; Flavonoids/metabolism ; Cytokines/metabolism ; Inflammation/metabolism ; Bronchoalveolar Lavage Fluid ; Oxidative Stress ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Mice, Inbred BALB C ; Disease Models, Animal
    Chemical Substances Ovalbumin (9006-59-1) ; myricetin (76XC01FTOJ) ; Flavonoids ; Cytokines ; Anti-Inflammatory Agents
    Language English
    Publishing date 2023-10-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1014929-6
    ISSN 1873-4847 ; 0955-2863
    ISSN (online) 1873-4847
    ISSN 0955-2863
    DOI 10.1016/j.jnutbio.2023.109485
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  5. Article: Hypoglycemic peptide‐enriched hydrolysates of Corbicula fluminea and Chlorella sorokiniana possess synergistic hypoglycemic activity through inhibiting α‐glucosidase and dipeptidyl peptidase‐4 activity

    Huang, Tse‐Hung / Liu, Pi‐Yu / Lin, Yu‐Ling / Tsai, Jenn‐Shou

    Journal of the science of food and agriculture. 2022 Jan. 30, v. 102, no. 2

    2022  

    Abstract: BACKGROUND: The prevalence of diabetes mellitus worldwide has increased in recent decades. Maintaining the level of blood glucose is the most basic and important issue for diabetics. This study aimed to investigate the hypoglycemic activity of a ... ...

    Abstract BACKGROUND: The prevalence of diabetes mellitus worldwide has increased in recent decades. Maintaining the level of blood glucose is the most basic and important issue for diabetics. This study aimed to investigate the hypoglycemic activity of a combination of hypoglycemic peptide‐enriched hydrolysates of Corbicula fluminea (ACH) and Chlorella sorokiniana (PCH). RESULTS: Combined supplementation of ACH and PCH synergistically inhibited α‐glucosidase and DPP4 activities in vitro. After 4 weeks of treatment with ACH and/or PCH, the plasma glucose concentration and insulin, homeostasis model assessment‐estimated insulin resistance (HOMA‐IR), total cholesterol (TC) and triglyceride (TG) levels significantly decreased. The hypoglycemic peptides in ACH and PCH were purified and assayed for α‐glucosidase and DPP4 activity. The hypoglycemic peptides in ACH and PCH effectively decreased α‐glucosidase and DPP4 activities. In silico assays showed that these two peptide types have different docking poses, which determined their inhibitory effect against α‐glucosidase and DPP4 activity. CONCLUSION: Combined treatment with hypoglycemic peptide‐enriched ACH and PCH could modulate blood glucose by synergistically inhibiting α‐glucosidase and DPP4 activities. © 2021 Society of Chemical Industry.
    Keywords Chlorella sorokiniana ; Corbicula fluminea ; agriculture ; blood glucose ; cholesterol ; computer simulation ; diabetes mellitus ; glycemic effect ; homeostasis ; insulin ; insulin resistance ; peptides ; triacylglycerols
    Language English
    Dates of publication 2022-0130
    Size p. 716-723.
    Publishing place John Wiley & Sons, Ltd.
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 184116-6
    ISSN 1097-0010 ; 0022-5142
    ISSN (online) 1097-0010
    ISSN 0022-5142
    DOI 10.1002/jsfa.11402
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  6. Article: Positive Effects of Laser Acupuncture in Methamphetamine Users Undergoing Group Cognitive Behavioral Therapy: A Pilot Study.

    Shiao, Yi-Hsien / Chen, Yi-Chih / Yeh, Yuan-Chieh / Huang, Tse-Hung

    Evidence-based complementary and alternative medicine : eCAM

    2021  Volume 2021, Page(s) 5514873

    Abstract: Background: Methamphetamine (MA) addiction has become a crucial public health concern because of its adverse consequences to individuals and the society.: Objective: To investigate the clinical efficacy of laser acupuncture combined with group ... ...

    Abstract Background: Methamphetamine (MA) addiction has become a crucial public health concern because of its adverse consequences to individuals and the society.
    Objective: To investigate the clinical efficacy of laser acupuncture combined with group cognitive behavioral therapy for MA addiction treatment.
    Materials and methods: MA users who participated in group cognitive behavioral therapy and met the inclusion criteria were referred from psychiatrists to participate. The participants received laser acupuncture treatment once a week for 2 months (total eight treatments) on selected acupoints (PC6, HT7, LI4, ST36, SP6, and LR3). Laboratory assessment included urinalysis for MA and liver function tests aspartate aminotransferase, alanine aminotransferase, and
    Result: Fifteen participants were enrolled, of whom seven completed the trial. Urinalysis for MA revealed a decrease in drug use from 57.1% to 28.6%. Compared with those before treatment, PSQI scores were significantly lower at 1 and 2 months after treatment (-3.73 and -4.10, respectively; both
    Conclusion: Laser acupuncture combined with group cognitive behavioral therapy may improve sleep quality, alleviate depression, and reduce MA use. Additional large-scale studies confirming the effectiveness of this modality are warranted.
    Language English
    Publishing date 2021-05-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2021/5514873
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Hypoglycemic peptide-enriched hydrolysates of Corbicula fluminea and Chlorella sorokiniana possess synergistic hypoglycemic activity through inhibiting α-glucosidase and dipeptidyl peptidase-4 activity.

    Huang, Tse-Hung / Liu, Pi-Yu / Lin, Yu-Ling / Tsai, Jenn-Shou

    Journal of the science of food and agriculture

    2021  Volume 102, Issue 2, Page(s) 716–723

    Abstract: Background: The prevalence of diabetes mellitus worldwide has increased in recent decades. Maintaining the level of blood glucose is the most basic and important issue for diabetics. This study aimed to investigate the hypoglycemic activity of a ... ...

    Abstract Background: The prevalence of diabetes mellitus worldwide has increased in recent decades. Maintaining the level of blood glucose is the most basic and important issue for diabetics. This study aimed to investigate the hypoglycemic activity of a combination of hypoglycemic peptide-enriched hydrolysates of Corbicula fluminea (ACH) and Chlorella sorokiniana (PCH).
    Results: Combined supplementation of ACH and PCH synergistically inhibited α-glucosidase and DPP4 activities in vitro. After 4 weeks of treatment with ACH and/or PCH, the plasma glucose concentration and insulin, homeostasis model assessment-estimated insulin resistance (HOMA-IR), total cholesterol (TC) and triglyceride (TG) levels significantly decreased. The hypoglycemic peptides in ACH and PCH were purified and assayed for α-glucosidase and DPP4 activity. The hypoglycemic peptides in ACH and PCH effectively decreased α-glucosidase and DPP4 activities. In silico assays showed that these two peptide types have different docking poses, which determined their inhibitory effect against α-glucosidase and DPP4 activity.
    Conclusion: Combined treatment with hypoglycemic peptide-enriched ACH and PCH could modulate blood glucose by synergistically inhibiting α-glucosidase and DPP4 activities. © 2021 Society of Chemical Industry.
    MeSH term(s) Animals ; Blood Glucose/metabolism ; Chlorella/chemistry ; Corbicula/chemistry ; Diabetes Mellitus, Type 2/drug therapy ; Diabetes Mellitus, Type 2/enzymology ; Diabetes Mellitus, Type 2/metabolism ; Dipeptidyl Peptidase 4/chemistry ; Dipeptidyl Peptidase 4/metabolism ; Dipeptidyl-Peptidase IV Inhibitors/administration & dosage ; Dipeptidyl-Peptidase IV Inhibitors/chemistry ; Drug Synergism ; Glycoside Hydrolase Inhibitors/administration & dosage ; Glycoside Hydrolase Inhibitors/chemistry ; Humans ; Hypoglycemic Agents/administration & dosage ; Hypoglycemic Agents/chemistry ; Male ; Peptides/administration & dosage ; Plant Extracts/administration & dosage ; Plant Extracts/chemistry ; Rats ; Rats, Sprague-Dawley ; alpha-Glucosidases/chemistry ; alpha-Glucosidases/metabolism
    Chemical Substances Blood Glucose ; Dipeptidyl-Peptidase IV Inhibitors ; Glycoside Hydrolase Inhibitors ; Hypoglycemic Agents ; Peptides ; Plant Extracts ; alpha-Glucosidases (EC 3.2.1.20) ; Dipeptidyl Peptidase 4 (EC 3.4.14.5)
    Language English
    Publishing date 2021-07-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 184116-6
    ISSN 1097-0010 ; 0022-5142
    ISSN (online) 1097-0010
    ISSN 0022-5142
    DOI 10.1002/jsfa.11402
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  8. Article: Melastoma malabathricum

    Huang, Tse-Hung / Hsieh, Pei-Wen / Chen, Tsu-Jung / Tsai, Hui-Ju / Cheng, Ju-Chien / Liao, Hsiang-Ruei / Kuo, Shun-Li / Tseng, Ching-Ping

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 2

    Abstract: Platelet hyper-reactivity and neutrophil extracellular trap (NET) formation contribute to the development of thromboembolic diseases for patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study investigated the ... ...

    Abstract Platelet hyper-reactivity and neutrophil extracellular trap (NET) formation contribute to the development of thromboembolic diseases for patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study investigated the pathophysiological effects of SARS-CoV-2 surface protein components and the viral double-stranded RNA (dsRNA) on platelet aggregation and NET formation. Traditional Chinese medicine (TCM) with anti-viral effects was also delineated. The treatment of human washed platelets with SARS-CoV-2 spike protein S1 or the ectodomain S1 + S2 regions neither caused platelet aggregation nor enhanced agonists-stimulated platelet aggregation. Moreover, NET formation can be induced by polyinosinic-polycytidylic acid (poly(I:C)), a synthetic analog of viral dsRNA, but not by the pseudovirus composed of SARS-CoV-2 spike, envelope, and membrane proteins. To search for TCM with anti-NET activity, the plant
    Language English
    Publishing date 2023-02-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12020341
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  9. Article: The Synergistic Effects of

    Huang, Tse-Hung / Liu, Bang-Hung / Hsu, Chia-Hui / Wu, Chang-Jer / Liao, Kuang-Wen / Lin, Chen-Si / Chan, Yi-Lin

    Antioxidants (Basel, Switzerland)

    2023  Volume 12, Issue 12

    Abstract: Osteoarthritis (OA) is a progressive disease that causes pain, stiffness, and inflammation in the affected joints. Currently, there are no effective treatments for preventing the worst outcomes, such as synovitis or cartilage degradation. ...

    Abstract Osteoarthritis (OA) is a progressive disease that causes pain, stiffness, and inflammation in the affected joints. Currently, there are no effective treatments for preventing the worst outcomes, such as synovitis or cartilage degradation.
    Language English
    Publishing date 2023-12-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox12122068
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  10. Article: Gamma-mangostin isolated from garcinia mangostana suppresses colon carcinogenesis and stemness by downregulating the GSK3β/β-catenin/CDK6 cancer stem pathway

    Wu, Alexander TH / Yeh, Yuan-Chieh / Huang, Yan-Jiun / Mokgautsi, Ntlotlang / Lawal, Bashir / Huang, Tse-Hung

    Phytomedicine. 2022 Jan., v. 95

    2022  

    Abstract: Despite advances in chemotherapies and targeted drugs, colorectal cancer (CRC) remains challenging to treat due to drug resistance. Emerging evidence indicates that cancer-associated fibroblasts (CAFs) facilitate the generation of cancer stem-like cells ( ...

    Abstract Despite advances in chemotherapies and targeted drugs, colorectal cancer (CRC) remains challenging to treat due to drug resistance. Emerging evidence indicates that cancer-associated fibroblasts (CAFs) facilitate the generation of cancer stem-like cells (CSCs) and drug resistance. Glycogen synthase kinase-3 (GSK) associated signaling pathways have been implicated in the generation of CSCs and represent a target for therapeutics development.Gamma-mangostin (gMG) isolated from Garcinia mangostana was evaluated for its ability to downregulate GSK3β-associated signaling in CRC cells and overcome CAF-induced 5-fluorouracil resistance and CSC generation.Bioinformatics analysis, in silico molecular docking, in vitro assays, including cell viability tests, colony- and tumor sphere-formation assays, transwell migration assays, ELISA, SDS-PAGE, Western blotting, miR expression, qPCR, and flow cytometry, as well as in vivo mouse xenograft models were used to evaluate the antitumor effects of gMG.Bioinformatics analyses indicated that GSK3β/CDK6/β-catenin mRNA signature was significantly higher in colon cancer patients. Additional algorithms predicted a higher miR-26b level was associated with significantly higher survival in CRC patients and GSK3β and CDK6 as targets of miR-26b-5p. To validate these findings in vitro, we showed that CAF-cocultured CRC cells expressed an increased expression of GSK3β, β-catenin, CDK6, and NF-κB. Therapeutically, we demonstrated that gMG treatment suppressed GSK3β-associated signaling pathways while concomitantly increased the miR-26b-5p level. Using a xenograft mouse model of CAFs cocultured HCT116 tumorspheres, we showed that gMG treatment reduced tumor growth and overcame CAF-induced 5-fluorouracil resistance.Pharmacological intervention with gMG suppressed CRC carcinogenesis and stemness via downregulating GSK3/β-catenin/CDK6 and upregulating the miR-26b-5p tumor suppressor. Thus, gMG represents a potential new CRC therapeutic agent and warrants further investigation.
    Keywords Garcinia mangostana ; carcinogenesis ; cell viability ; colon ; colorectal neoplasms ; computer simulation ; drug resistance ; fibroblasts ; flow cytometry ; fluorouracil ; glycogen (starch) synthase ; mice ; polyacrylamide gel electrophoresis ; xenotransplantation
    Language English
    Dates of publication 2022-01
    Publishing place Elsevier GmbH
    Document type Article
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2021.153797
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