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  1. Article ; Online: Cattle Target Segmentation Method in Multi-Scenes Using Improved DeepLabV3+ Method

    Feng, Tao / Guo, Yangyang / Huang, Xiaoping / Qiao, Yongliang

    Animals. 2023 Aug. 04, v. 13, no. 15

    2023  

    Abstract: Obtaining animal regions and the relative position relationship of animals in the scene is conducive to further studying animal habits, which is of great significance for smart animal farming. However, the complex breeding environment still makes ... ...

    Abstract Obtaining animal regions and the relative position relationship of animals in the scene is conducive to further studying animal habits, which is of great significance for smart animal farming. However, the complex breeding environment still makes detection difficult. To address the problems of poor target segmentation effects and the weak generalization ability of existing semantic segmentation models in complex scenes, a semantic segmentation model based on an improved DeepLabV3+ network (Imp-DeepLabV3+) was proposed. Firstly, the backbone network of the DeepLabV3+ model was replaced by MobileNetV2 to enhance the feature extraction capability of the model. Then, the layer-by-layer feature fusion method was adopted in the Decoder stage to integrate high-level semantic feature information with low-level high-resolution feature information at multi-scale to achieve more precise up-sampling operation. Finally, the SENet module was further introduced into the network to enhance information interaction after feature fusion and improve the segmentation precision of the model under complex datasets. The experimental results demonstrate that the Imp-DeepLabV3+ model achieved a high pixel accuracy (PA) of 99.4%, a mean pixel accuracy (MPA) of 98.1%, and a mean intersection over union (MIoU) of 96.8%. Compared to the original DeepLabV3+ model, the segmentation performance of the improved model significantly improved. Moreover, the overall segmentation performance of the Imp-DeepLabV3+ model surpassed that of other commonly used semantic segmentation models, such as Fully Convolutional Networks (FCNs), Lite Reduced Atrous Spatial Pyramid Pooling (LR-ASPP), and U-Net. Therefore, this study can be applied to the field of scene segmentation and is conducive to further analyzing individual information and promoting the development of intelligent animal farming.
    Keywords cattle ; data collection ; models
    Language English
    Dates of publication 2023-0804
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13152521
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Cattle Target Segmentation Method in Multi-Scenes Using Improved DeepLabV3+ Method.

    Feng, Tao / Guo, Yangyang / Huang, Xiaoping / Qiao, Yongliang

    Animals : an open access journal from MDPI

    2023  Volume 13, Issue 15

    Abstract: Obtaining animal regions and the relative position relationship of animals in the scene is conducive to further studying animal habits, which is of great significance for smart animal farming. However, the complex breeding environment still makes ... ...

    Abstract Obtaining animal regions and the relative position relationship of animals in the scene is conducive to further studying animal habits, which is of great significance for smart animal farming. However, the complex breeding environment still makes detection difficult. To address the problems of poor target segmentation effects and the weak generalization ability of existing semantic segmentation models in complex scenes, a semantic segmentation model based on an improved DeepLabV3+ network (Imp-DeepLabV3+) was proposed. Firstly, the backbone network of the DeepLabV3+ model was replaced by MobileNetV2 to enhance the feature extraction capability of the model. Then, the layer-by-layer feature fusion method was adopted in the Decoder stage to integrate high-level semantic feature information with low-level high-resolution feature information at multi-scale to achieve more precise up-sampling operation. Finally, the SENet module was further introduced into the network to enhance information interaction after feature fusion and improve the segmentation precision of the model under complex datasets. The experimental results demonstrate that the Imp-DeepLabV3+ model achieved a high pixel accuracy (
    Language English
    Publishing date 2023-08-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13152521
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Comparing the immune response and protective effect of COVID-19 vaccine under different vaccination strategies.

    Zhao, Tianyi / Huang, Xiaoping / Shu, Yuelong

    Human vaccines & immunotherapeutics

    2023  Volume 19, Issue 3, Page(s) 2273155

    Abstract: Although highly infectious respiratory viral infections spread rapidly, humans have evolved a precise and complex immune mechanism to deal with respiratory viruses, with strong intrinsic, highly adaptive and specific humoral and cellular immunity. At the ...

    Abstract Although highly infectious respiratory viral infections spread rapidly, humans have evolved a precise and complex immune mechanism to deal with respiratory viruses, with strong intrinsic, highly adaptive and specific humoral and cellular immunity. At the same time, vaccination against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is one of the most cost-effective and efficient means of preventing morbidity, severe illness, and death from Coronavirus disease 2019 (COVID-19). As the global epidemic of COVID-19 continues to evolve and vaccines are being developed, it is important to conduct studies on immunization strategies to optimize vaccination strategies when appropriate. This review was conducted to investigate the relationship between the immune response and the protective effect of different vaccination scenarios (including booster, sequential and hybrid immunity), and to provide a basis for the optimization of vaccination strategies and the development of new vaccines in the future.
    MeSH term(s) Humans ; COVID-19 Vaccines ; COVID-19/prevention & control ; SARS-CoV-2 ; Viral Vaccines ; Vaccination ; Immunity, Cellular ; Antibodies, Viral
    Chemical Substances COVID-19 Vaccines ; Viral Vaccines ; Antibodies, Viral
    Language English
    Publishing date 2023-12-19
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2664176-8
    ISSN 2164-554X ; 2164-5515
    ISSN (online) 2164-554X
    ISSN 2164-5515
    DOI 10.1080/21645515.2023.2273155
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: miR-940 modulates CD47 to suppress biological functions of lung adenocarcinoma cells.

    Long, Shuzi / Long, Xizi / Guo, Jing / Fu, Liping / Huang, Xiaoping / Liu, Huawen

    American journal of cancer research

    2024  Volume 14, Issue 3, Page(s) 1157–1173

    Abstract: Objective: mir-940 and CD47 play regulatory and immunoregulatory roles in lung cancer. While previous study found that the expression of mir-940 decreased, associated with the increasing of CD47 in lung adenocarcinoma. However, their inherent ... ...

    Abstract Objective: mir-940 and CD47 play regulatory and immunoregulatory roles in lung cancer. While previous study found that the expression of mir-940 decreased, associated with the increasing of CD47 in lung adenocarcinoma. However, their inherent correlations remain elusive. Herein, this experiment intends to search for the relevant molecular mechanisms regulating the biological function of non-small cell lung cancer.
    Methods: The cancer and adjacent tissue samples were collected from 20 pairs of newly diagnosed non-small cell lung cancer patients without applying radiotherapy and chemotherapy. We performed immunohistochemistry containing 45 lung adenocarcinoma tissues to investigate the relationship between the clinicopathological features and CD47 expression. The expressions of
    Results: CD47 expression was not associated with clinicopathologic factors in lung adenocarcinoma. The proliferation, migration, invasion, and cloning abilities of lung adenocarcinoma cells were weakened after transfection with mir-940 mimic and siRNA-CD47. Overexpression of CD47 could promote proliferation, migration, invasion, cloning abilities, reduce apoptosis rate and attenuate the antitumor effect of mir-940 on lung adenocarcinoma. Dual luciferase experiments confirmed that mir-940 can target CD47 molecules.
    Conclusion: mir-940 can inhibit the biological function of lung adenocarcinoma cells by targeting CD47.
    Language English
    Publishing date 2024-03-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2589522-9
    ISSN 2156-6976
    ISSN 2156-6976
    DOI 10.62347/YYJN3674
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Estuarine hydrodynamic processes driving the molecular changes of terrestrial dissolved organic nitrogen: From mixing to biological modification.

    Li, Jinlong / Wu, Yunchao / Yang, Jia / Li, Pengju / Jiang, Zhijian / Liu, Songlin / Huang, Xiaoping

    The Science of the total environment

    2024  Volume 917, Page(s) 170489

    Abstract: Estuaries receive substantial amounts of terrestrial dissolved organic nitrogen (tDON), which will be transported from the freshwater to the oceanic terminus through vigorous exchange processes. However, the intricate migration and transformation ... ...

    Abstract Estuaries receive substantial amounts of terrestrial dissolved organic nitrogen (tDON), which will be transported from the freshwater to the oceanic terminus through vigorous exchange processes. However, the intricate migration and transformation dynamics of tDON during this transportation, particularly at a molecular level, remain constrained. To address this knowledge gap, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) was used for the analysis of DON molecular composition in the Pearl River Estuary (PRE), a river-dominated estuarine system influenced by intensified anthropogenic activities in southern China. The results showed a pronounced spatial-temporal variation in DON concentration in the study area. At the molecular level, tDON exhibited reduced unsaturation and aromaticity, coupled with an elevated abundance of DON compounds containing one‑nitrogen atom (1 N-DON, 53.17 %) and compounds containing carbon, hydrogen, oxygen, nitrogen, and sulfur (CHONS) (27.46 %). It was evident that lignin was depleted while more oxygenated tannin compounds were generated in the freshwater-seawater mixing zone. This transformation is attributed to heightened biological activities, likely influenced by the priming effect of terrestrial nutrient inputs. In summer, the prevailing plume combined with biological activities in the strong mixing area and outer estuary increased the abundance of 3 N-DON molecules and a concurrent rise in the abundance of DON compounds containing only carbon, hydrogen, oxygen, and nitrogen (CHON), DON compounds containing carbon, hydrogen, oxygen, nitrogen, sulfur, and phosphorus (CHONSP), and CHONS. This trend also underscores the expanding role of marine plankton and microbes in the utilization of DON compounds containing carbon, hydrogen, oxygen, nitrogen, and phosphorus (CHONP). These findings provide details of tDON transformation processes at the molecular level in a river-dominated estuary and underline the estuarine hydrodynamics involved in transporting and altering DON within the estuary.
    MeSH term(s) Dissolved Organic Matter ; Hydrodynamics ; Nitrogen/analysis ; Rivers ; Estuaries ; Carbon/analysis ; Oxygen/analysis ; Sulfur/analysis ; Hydrogen/analysis ; Phosphorus/analysis
    Chemical Substances Dissolved Organic Matter ; Nitrogen (N762921K75) ; Carbon (7440-44-0) ; Oxygen (S88TT14065) ; Sulfur (70FD1KFU70) ; Hydrogen (7YNJ3PO35Z) ; Phosphorus (27YLU75U4W)
    Language English
    Publishing date 2024-02-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2024.170489
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Hydrodynamics and dissolved organic matter components shaped the fate of dissolved heavy metals in an intensely anthropogenically disturbed estuary.

    Zhang, Ling / Wu, Yunchao / Li, Jinlong / Ni, Zhixin / Ren, Yuzheng / Lin, Jizhen / Huang, Xiaoping

    The Science of the total environment

    2024  Volume 934, Page(s) 173293

    Abstract: Anthropogenic activities and natural erosion caused abundant influx of heavy metals (HMs) and organic matter (OM) into estuaries characterized by the dynamic environments governed by tidal action and river flow. Similarities and differences in the fate ... ...

    Abstract Anthropogenic activities and natural erosion caused abundant influx of heavy metals (HMs) and organic matter (OM) into estuaries characterized by the dynamic environments governed by tidal action and river flow. Similarities and differences in the fate of HM and OM as well as the influences of OM on HMs remain incomplete in estuaries with seasonal human activity and hydrodynamic force. To address this gap, dissolved HMs (dHMs) and fluorescence dissolved OM (FDOM) were investigated in the Pearl River Estuary, a highly seasonally anthropogenic and dynamic estuary. It aimed to elucidate the effects of hydrodynamic conditions and DOM on the seasonal fate of dHMs via the multivariate statistical methods. Our findings indicated dHMs and FDOM exhibited consistently higher levels in the upper estuarine and coastal waters in both seasons, predominantly controlled by the terrestrial/anthropogenic discharge. In the wet season, dHMs and humic-like substances (HULIS) were positively correlated, showing that dHMs readily combined with HULIS. This association led to a synchronous decrease offshore along the axis of the estuary and the transport following the river plume in the surface affected by the salt wedge. Contrarily, dHMs were prone to complex with protein-like components impacted by the hydrodynamics during the dry season. Principal component analysis (PCA) results revealed the terrestrial/anthropogenic inputs and the fresh-seawater mixing process were the most crucial factors responsible for the fate of dHM in wet and dry seasons, respectively, with DOM identified as a secondary but significant influencing factor in both seasons. This study holds significance in providing valuable insights into the migration, transformation, the ultimate fate of dHMs in anthropogenically influenced estuaries, as well as the intricate dynamics governing coastal ecosystems.
    Language English
    Publishing date 2024-05-15
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2024.173293
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Psychological Burden of Patients with Head and Neck Cancer Undergoing Radiotherapy and Their Family Caregivers: A Cross-Sectional Survey.

    Tang, Yuanyuan / Hua, Yuchen / Huang, Xiaoping / Cao, Yuandong / Sun, Xinchen

    Journal of multidisciplinary healthcare

    2023  Volume 16, Page(s) 927–935

    Abstract: Background: The consequences of Head and neck cancer (HNC) affect both the person who receives the diagnosis and their family caregivers (FCs).: Objective: To investigate the psychological status of patients and their FCs, and the burden of the FCs ... ...

    Abstract Background: The consequences of Head and neck cancer (HNC) affect both the person who receives the diagnosis and their family caregivers (FCs).
    Objective: To investigate the psychological status of patients and their FCs, and the burden of the FCs during radiotherapy.
    Methods: This cross-sectional study was conducted with a questionnaire survey by convenience sampling method. Patients with HNC and their caregivers (both N = 85) from the radiotherapy department of our hospital were recruited between March 2021 and March 2022. The Hospital Anxiety and Depression Scale (HADS), the Chinese version of the Connor and Davidson Resilience Scale (CD-RISC), and the Zarit Burden Interview (ZBI) were used to assess the symptoms of anxiety and depression, psychological resilience, and the impact of care work, emotions and social lives of participants. Pearson's correlation analysis and a Mann-Whitney test were used to analyse the association between the HADS and the CD-RISC scores of the patients.
    Results: About half of the patients (56.47%) and the caregivers (62.35%) have had anxiety. The average HADS-Anxiety scores, HADS-Depression scores, and CD-RISC scores of the patients with HNC were 7.4±1.9, 6.4±2.2, and 56.8±12.6. The "Strength" and "Resilience" scores of the patients were inversely related to their HADS anxiety scores (
    Conclusion: More than half of patients with HNC undergoing radiotherapy have anxiety, and about a third have depression. The anxiety and depression status of the FCs of patients with HNC undergoing radiotherapy is related to caregiver burden, deserving the attention of clinical medical staff.
    Language English
    Publishing date 2023-04-05
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2453343-9
    ISSN 1178-2390
    ISSN 1178-2390
    DOI 10.2147/JMDH.S398064
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: An analysis of sintilimab combined with ruxolitinib as compassionate therapy for 12 adults with EBV-associated hemophagocytic lymphohistiocytosis.

    Xu, Ying / Li, Wenting / Gan, Jianhe / He, Xuefeng / Huang, Xiaoping

    Annals of hematology

    2023  Volume 102, Issue 12, Page(s) 3325–3333

    Abstract: Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH) is a severe hyperinflammatory illness that affects adults and is caused by an EBV infection. Without allogeneic hematopoietic stem cell transplantation (allo-HSCT), the overall ... ...

    Abstract Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH) is a severe hyperinflammatory illness that affects adults and is caused by an EBV infection. Without allogeneic hematopoietic stem cell transplantation (allo-HSCT), the overall survival of adult patients with EBV-HLH is unsatisfactory, necessitating the development of innovative therapeutic approaches. The clinical records of twelve EBV-HLH patients who received sintilimab therapy combined with ruxolitinib on a compassionate basis at the First Affiliated Hospital of Soochow University were retrospectively examined in this investigation. All the patients responded without fever, but three patients relapsed within a week. Among the nine patients achieving complete response (CR), 55.6% (5/9) maintained CR for >4.5 months, and 33.3% (3/9) relapsed following CR. Neither patients with no response (NR) nor relapsed patients were fit for allo-HSCT, and all died soon after discharge. Six patients had clinical CR with a median follow-up of 5 (4.4-14.7) months. There were no documented severe negative effects. Additional information on this innovative treatment for adult EBV-HLH is provided in our report.
    MeSH term(s) Humans ; Adult ; Lymphohistiocytosis, Hemophagocytic/complications ; Lymphohistiocytosis, Hemophagocytic/drug therapy ; Herpesvirus 4, Human ; Epstein-Barr Virus Infections/complications ; Epstein-Barr Virus Infections/drug therapy ; Retrospective Studies
    Chemical Substances ruxolitinib (82S8X8XX8H) ; sintilimab (8FU7FQ8UPK)
    Language English
    Publishing date 2023-10-03
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1064950-5
    ISSN 1432-0584 ; 0939-5555 ; 0945-8077
    ISSN (online) 1432-0584
    ISSN 0939-5555 ; 0945-8077
    DOI 10.1007/s00277-023-05476-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: [Advances in the plant multicellular network analysis].

    Shi, Bore / Huang, Xiaoping / Fu, Xiurong / Wang, Bangjun

    Sheng wu gong cheng xue bao = Chinese journal of biotechnology

    2022  Volume 38, Issue 8, Page(s) 2798–2810

    Abstract: Multicellular network analysis is a method for topological properties analysis of cells. The functions of organs are determined by their inner cells. The arrangement of cells within organs endows higher-order functionality through a structure-function ... ...

    Abstract Multicellular network analysis is a method for topological properties analysis of cells. The functions of organs are determined by their inner cells. The arrangement of cells within organs endows higher-order functionality through a structure-function relationship, though the organizational properties of these multicellular configurations remain poorly understood. Multicellular network analysis with multicellular models established by 3D scanning of plants, will further discover the plant development mechanism, and provide clues for synthesizing plant multicellular systems. In this paper, we review the development of multicellular models, summarize the process of multicellular network analysis, and describe the development and application of multicellular network analysis in plants. In addition, this review also provides perspective on future development of plant multicellular network analysis.
    MeSH term(s) Plants
    Language Chinese
    Publishing date 2022-08-25
    Publishing country China
    Document type Journal Article ; Review
    ZDB-ID 1042206-7
    ISSN 1872-2075 ; 1042-749X
    ISSN (online) 1872-2075
    ISSN 1042-749X
    DOI 10.13345/j.cjb.220127
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Reactive oxygen species enhance rAAV transduction by promoting its escape from late endosomes

    Huang, Xiaoping / Wang, Xiao / Ren, Yanxuan / Gao, Pingzhang / Xu, Wentao / Xie, Xiaolan / Diao, Yong

    Virol J. 2023 Dec., v. 20, no. 1 p.2-2

    2023  

    Abstract: BACKGROUND: Recent seminal studies have revealed that endosomal reactive oxygen species (ROS) promote rather than inhibit viral infection. Some ROS generators, including shikonin and H₂O₂, have the potential to enhance recombinant adeno-associated virus ( ...

    Abstract BACKGROUND: Recent seminal studies have revealed that endosomal reactive oxygen species (ROS) promote rather than inhibit viral infection. Some ROS generators, including shikonin and H₂O₂, have the potential to enhance recombinant adeno-associated virus (rAAV) transduction. However, the impact of ROS on rAAV intracellular trafficking remains unclear. METHODS: To understand the effects of ROS on the transduction of rAAV vectors, especially the rAAV subcellular distribution profiles, this study systematically explored the effect of ROS on each step of rAAV intracellular trafficking pathway using fluorescently-labeled rAAV and qPCR quantification determination. RESULTS: The results showed promoted in-vivo and in-vitro rAAV transduction by ROS exposure, regardless of vector serotype or cell type. ROS treatment directed rAAV intracellular trafficking towards a more productive pathway by upregulating the expression of cathepsins B and L, accelerating the rAAV transit in late endosomes, and increasing the rAAV nucleus entry. CONCLUSIONS: These data support that ROS generative drugs, such as shikonin, have the potential to promote rAAV vector transduction by promoting rAAV’s escape from late endosomes, and enhancing its productive trafficking to the nucleus.
    Keywords Dependoparvovirus ; cathepsins ; endosomes ; reactive oxygen species ; serotypes ; shikonin
    Language English
    Dates of publication 2023-12
    Size p. 2.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2160640-7
    ISSN 1743-422X
    ISSN 1743-422X
    DOI 10.1186/s12985-023-01964-w
    Database NAL-Catalogue (AGRICOLA)

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