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Article ; Online: Spindle‐E is essential for gametogenesis in the silkworm, Bombyx mori

Chen, Kai / Yang, Xu / Yang, Dehong / Huang, Yongping

Insect Science. 2023 Apr., v. 30, no. 2 p.293-304

2023

Abstract: As a defense mechanism against transposable elements, the PIWI‐interacting RNA (piRNA) pathway maintains genomic integrity and ensures proper gametogenesis in gonads. Numerous factors are orchestrated to ensure normal operation of the piRNA pathway. ... ...

Abstract	As a defense mechanism against transposable elements, the PIWI‐interacting RNA (piRNA) pathway maintains genomic integrity and ensures proper gametogenesis in gonads. Numerous factors are orchestrated to ensure normal operation of the piRNA pathway. Spindle‐E (Spn‐E) gene was one of the first genes shown to participate in the piRNA pathway. In this study, we performed functional analysis of Spn‐E in the model lepidopteran insect, Bombyx mori. Unlike the germline‐specific expression pattern observed in Drosophila and mouse, BmSpn‐E was ubiquitously expressed in all tissues tested, and it was highly expressed in gonads. Immunofluorescent staining showed that BmSpn‐E was localized in both germ cells and somatic cells in ovary and was expressed in spermatocytes in testis. We used a binary transgenic CRISPR/Cas9 system to construct BmSpn‐E mutants. Loss of BmSpn‐E expression caused derepression of transposons in gonads. We also found that mutant gonads were much smaller than wild‐type gonads and that the number of germ cells was considerably lower in mutant gonads. Quantitative real‐time PCR analysis and TUNEL staining revealed that apoptosis was greatly enhanced in mutant gonads. Further, we found that the BmSpn‐E mutation impacted gonadal development and gametogenesis at the early larval stage. In summary, our data provided the first evidence that BmSpn‐E plays vital roles in gonadal development and gametogenesis in B. mori.
Keywords	Bombyx mori ; CRISPR-Cas systems ; Drosophila ; apoptosis ; gametogenesis ; genes ; genetically modified organisms ; genomics ; larvae ; mice ; mutants ; mutation ; quantitative polymerase chain reaction ; silkworms ; small interfering RNA ; spermatocytes ; testes ; transposons
Language	English
Dates of publication	2023-04
Size	p. 293-304.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2179775-4
ISSN	1744-7917 ; 1672-9609
ISSN (online)	1744-7917
ISSN	1672-9609
DOI	10.1111/1744-7917.13096
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: WISCO’s Low-carbon Transformation Based On LEAP And Scenario Analysis

Huang Yongping / Wang Ben / Yang Wu / Sun Lushi

E3S Web of Conferences, Vol 350, p

2022 Volume 03004

Abstract: The paper takes Wuhan Iron and Steel Co., Ltd. (hereinafter referred to as “ WISCO “) energy consumption and carbon emissions as the research object, and analyzes WISCO’s carbon emissions. Through the construction of WISCO’s Long range Energy ... ...

Abstract	The paper takes Wuhan Iron and Steel Co., Ltd. (hereinafter referred to as “ WISCO “) energy consumption and carbon emissions as the research object, and analyzes WISCO’s carbon emissions. Through the construction of WISCO’s Long range Energy Alternatives Planning system (hereinafter referred to as LEAP) prediction model for energy consumption and carbon emissions, three scenarios have been set up: a baseline scenario, an energy efficiency improvement scenario, and an energy structure change scenario to measure WISCO’s carbon emissions. Based on the results of the LEAP model, this article puts forward the following recommendations for WISCO’s low-carbon transformation. WISCO must strictly control steel output.WISCO should introduce electric furnaces to increase the proportion of electric furnace steel. While ensuring the supply of scrap steel resources, WISCO ought to use scrap steel efficiently. In addition, introduction of hydrogen direct reduction iron technology is also crucial to WISCO in a planned way.
Keywords	Environmental sciences ; GE1-350
Subject code	690
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Charging and discharging enhancement of a vertical latent heat storage unit by fractal tree-shaped fins

Huang, Yongping / Liu, Xiangdong

Renewable energy. 2021 Aug., v. 174

2021

Abstract: The popular application of latent heat storage (LHS) units using phase change materials is limited in converting and utilizing renewable energy due to their poor thermal efficiency. To address this deficiency, a tree-shaped fin inspired by nature is ... ...

Abstract	The popular application of latent heat storage (LHS) units using phase change materials is limited in converting and utilizing renewable energy due to their poor thermal efficiency. To address this deficiency, a tree-shaped fin inspired by nature is employed for the thermal enhancement of vertical LHS units. A three-dimensional mathematical model using the enthalpy-porosity method is developed to comprehensively evaluate the heat charging/discharging process of the new and traditional LHS units, focusing on the role of heat transfer fluid (HTF) direction. The results indicate that tree-shaped fins improve the temperature uniformity and facilitate the melting/solidification rate, which exhibits a preferable advantage that the full melting/solidification duration shortens by 34.5% and 49.2%, and the time-averaged heat storage/release rate augments by 49.4% and 96.4%. Interestingly, natural convection significantly improves the heat charging performance, while the role of natural convection can be considered negligible during discharging processes. Moreover, the HTF temperature seriously affects the performance of LHS units, while the role of HTF flow rate is less prominent. It is found that the upward flow of HTF is beneficial to the heat charging improvement while a downward flow mode facilitates the discharging performance, providing useful advice for practical applications of vertical LHS units.
Keywords	convection ; latent heat ; mathematical models ; renewable energy sources ; solidification ; temperature
Language	English
Dates of publication	2021-08
Size	p. 199-217.
Publishing place	Elsevier Ltd
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2001449-1
ISSN	1879-0682 ; 0960-1481
ISSN (online)	1879-0682
ISSN	0960-1481
DOI	10.1016/j.renene.2021.04.066
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Super amplification enabled by orbital angular momentum in weak measurement.

Xie, Shiwei / Zhu, Junfan / Wang, An / Wang, Yue / Huang, Yongping / Zhang, Zhiyou

Optics express

2024 Volume 32, Issue 7, Page(s) 11794–11800

Abstract: Weak measurement, which can amplify a weak signal, has shown great significance in precision measurements. The amplification is usually realized through the weak value and the propagation factor. We show that the orbital angular momentum (OAM) can ... ...

Abstract	Weak measurement, which can amplify a weak signal, has shown great significance in precision measurements. The amplification is usually realized through the weak value and the propagation factor. We show that the orbital angular momentum (OAM) can provide another dimension for amplification that is linearly proportional to the OAM number. We employ OAM to measure the spin Hall effect of light and demonstrate that the OAM-enabled amplification is compatible with the weak value amplification and the propagation amplification. This work is probable to promote the application of OAM in precision measurements.
Language	English
Publishing date	2024-04-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.517987
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Spindle-E is essential for gametogenesis in the silkworm, Bombyx mori.

Chen, Kai / Yang, Xu / Yang, Dehong / Huang, Yongping

Insect science

2022 Volume 30, Issue 2, Page(s) 293–304

Abstract: As a defense mechanism against transposable elements, the PIWI-interacting RNA (piRNA) pathway maintains genomic integrity and ensures proper gametogenesis in gonads. Numerous factors are orchestrated to ensure normal operation of the piRNA pathway. ... ...

Abstract	As a defense mechanism against transposable elements, the PIWI-interacting RNA (piRNA) pathway maintains genomic integrity and ensures proper gametogenesis in gonads. Numerous factors are orchestrated to ensure normal operation of the piRNA pathway. Spindle-E (Spn-E) gene was one of the first genes shown to participate in the piRNA pathway. In this study, we performed functional analysis of Spn-E in the model lepidopteran insect, Bombyx mori. Unlike the germline-specific expression pattern observed in Drosophila and mouse, BmSpn-E was ubiquitously expressed in all tissues tested, and it was highly expressed in gonads. Immunofluorescent staining showed that BmSpn-E was localized in both germ cells and somatic cells in ovary and was expressed in spermatocytes in testis. We used a binary transgenic CRISPR/Cas9 system to construct BmSpn-E mutants. Loss of BmSpn-E expression caused derepression of transposons in gonads. We also found that mutant gonads were much smaller than wild-type gonads and that the number of germ cells was considerably lower in mutant gonads. Quantitative real-time PCR analysis and TUNEL staining revealed that apoptosis was greatly enhanced in mutant gonads. Further, we found that the BmSpn-E mutation impacted gonadal development and gametogenesis at the early larval stage. In summary, our data provided the first evidence that BmSpn-E plays vital roles in gonadal development and gametogenesis in B. mori.
MeSH term(s)	Male ; Female ; Animals ; Mice ; Bombyx/genetics ; Bombyx/metabolism ; RNA, Small Interfering/metabolism ; Gonads/metabolism ; Gametogenesis ; Ovary/metabolism ; Drosophila/genetics ; Piwi-Interacting RNA
Chemical Substances	RNA, Small Interfering ; Piwi-Interacting RNA
Language	English
Publishing date	2022-08-11
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2179775-4
ISSN	1744-7917 ; 1672-9609
ISSN (online)	1744-7917
ISSN	1672-9609
DOI	10.1111/1744-7917.13096
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Numerical study on the thermal enhancement of horizontal latent heat storage units with hierarchical fins

Huang, Yongping / Yao, Feng / Liu, Xiangdong

Renewable energy. 2021 Dec., v. 180

2021

Abstract: The poor thermal performance of shell and tube latent heat storage (LHS) units using phase change materials restricts large-scale renewable energy utilisation. To address this issue, an innovative hierarchical fin is designed here to improve the charging ...

Abstract	The poor thermal performance of shell and tube latent heat storage (LHS) units using phase change materials restricts large-scale renewable energy utilisation. To address this issue, an innovative hierarchical fin is designed here to improve the charging performance of horizontal LHS units. The charging characteristics of innovative LHS units are explored and compared with available LHS units by a numerical model using the enthalpy-porosity scheme. Moreover, the response surface method (RSM) is applied to optimise the hierarchical fin by maximising its charging rate. The results indicate that hierarchical fins significantly improve the charging rate and temperature uniformity of horizontal LHS units. Except for the early heat conduction stage, melting convection develops as the dominant mode in the middle stage but decays in the later stage. Compared to tree-shaped fins, the hierarchical fin dramatically enhances heat conduction performance in the late melting stage, leading to a maximum reduction of 41.1% in the whole melting time of horizontal LHS units. To maximise the charging performance, the RSM results suggest the thickness gradient of the primary bifurcation, thickness fractal dimension, and length ratio of hierarchical fins should be 3.98, 1.34, and 1.11, respectively, providing constructive guidance for engineering applications of horizontal LHS units.
Keywords	convection ; fractal dimensions ; latent heat ; mathematical models ; renewable energy sources ; response surface methodology ; temperature
Language	English
Dates of publication	2021-12
Size	p. 383-397.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2001449-1
ISSN	1879-0682 ; 0960-1481
ISSN (online)	1879-0682
ISSN	0960-1481
DOI	10.1016/j.renene.2021.08.100
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Structure and characteristics of the plant-frugivore bird network from the Guilin Botanical Garden.

Wang, Guohai / Huang, Yang / Yao, Wei / Huang, Qiuchan / Huang, Yongping / Wei, Lijuan / Zhou, Qihai

PeerJ

2023 Volume 11, Page(s) e15028

Abstract: The interaction between plants and frugivores is crucial to ecosystem function and community diversity. However, little is known about the interaction between plants and frugivorous bird species in urban green spaces. We observed interactions between ... ...

Abstract	The interaction between plants and frugivores is crucial to ecosystem function and community diversity. However, little is known about the interaction between plants and frugivorous bird species in urban green spaces. We observed interactions between plants and frugivorous birds in the Guilin Botanical Garden for one year and determined the structure and characteristics of the interaction network. We also analyzed the impact of species traits on their network roles. Interactions between 14 frugivorous birds and 13 fruit plant species were recorded in the study area. Autumn interactions comprised 38.79% of the overall network, and winter interactions comprised 33.15%. The modularity (
MeSH term(s)	Animals ; Ecosystem ; Feeding Behavior ; Fruit ; Plants ; Birds
Language	English
Publishing date	2023-03-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2703241-3
ISSN	2167-8359 ; 2167-8359
ISSN (online)	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.15028
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A Supramolecular Reinforced Gel Fracturing Fluid with Low Permeability Damage Applied in Deep Reservoir Hydraulic Fracturing.

Huang, Yongping / Yao, Xinlong / Dai, Caili / Wu, Yining / Li, Lin / Yuan, Bin

Gels (Basel, Switzerland)

2023 Volume 10, Issue 1

Abstract: Gel fracturing fluid is the optimum fracturing fluid for proppant suspension, which is commonly applied in deep reservoir hydraulic fracturing. The content of polymers and crosslinkers in gel fracturing fluid is usually high to meet the needs of high- ... ...

Abstract	Gel fracturing fluid is the optimum fracturing fluid for proppant suspension, which is commonly applied in deep reservoir hydraulic fracturing. The content of polymers and crosslinkers in gel fracturing fluid is usually high to meet the needs of high-temperature resistance, leading to high costs and reservoir permeability damage caused by incomplete gel-breaking. In this paper, a supramolecular reinforced gel (SRG) fracturing fluid was constructed by strengthening the supramolecular force between polymers. Compared with single network gel (SNG) fracturing fluid, SRG fracturing fluid could possess high elasticity modulus (G' = 12.20 Pa) at lower polymer (0.4 wt%) and crosslinker (0.1 wt%) concentrations. The final viscosity of SRG fracturing fluid was 72.35 mPa·s, meeting the temperature resistance requirement of gel fracturing fluid at 200 °C. The gel-breaking time could be extended to 90-120 min using an encapsulated gel breaker. Gel particles are formed after the gel fracturing fluid is broken. The median particle size of gel particles in the SRG-breaking solution was 126 nm, which was much smaller than that in the industrial gel (IDG) breaking fluid (587 nm). The damage of the SRG-breaking solution to the core permeability was much less than the IDG-breaking solution. The permeability damage of cores caused by the SRG-breaking solutions was only about half that of IDG-breaking solutions at 1 mD.
Language	English
Publishing date	2023-12-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2813982-3
ISSN	2310-2861 ; 2310-2861
ISSN (online)	2310-2861
ISSN	2310-2861
DOI	10.3390/gels10010002
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Article: Comparison of physicochemical properties of different tissues from China climbing perch

Luo, Qiulan / Zha, Guangcai / Lin, Liyun / Huang, Yongping / Zou, Xianghui

Food science & nutrition

2022 Volume 10, Issue 3, Page(s) 936–944

Abstract: This study aimed to investigate nutrition in climbing ... ...

Abstract	This study aimed to investigate nutrition in climbing perch
Language	English
Publishing date	2022-01-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703010-6
ISSN	2048-7177
ISSN	2048-7177
DOI	10.1002/fsn3.2727
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Article ; Online: Genome-wide identification of yellow gene family in Hermetia illucens and functional analysis of yellow-y by CRISPR/Cas9.

Dong, Yongcheng / Xu, Xiaomiao / Qian, Lansa / Kou, Zongqing / Andongma, Awawing A / Zhou, Lijun / Huang, Yongping / Wang, Yaohui

Insect science

2024

Abstract: The yellow gene family plays a crucial role in insect pigmentation. It has potential for use as a visible marker gene in genetic manipulation and transgenic engineering in several model and non-model insects. Sadly, yellow genes have rarely been ... ...

Abstract	The yellow gene family plays a crucial role in insect pigmentation. It has potential for use as a visible marker gene in genetic manipulation and transgenic engineering in several model and non-model insects. Sadly, yellow genes have rarely been identified in Stratiomyidae species and the functions of yellow genes are relatively unknown. In the present study, we first manually annotated and curated 10 yellow genes in the black soldier fly (BSF), Hermetia illucens (Stratiomyidae). Then, the conserved amino acids in the major royal jelly proteins (MRJPs) domain, structural architecture and phylogenetic relationship of yellow genes in BSF were analyzed. We found that the BSF yellow-y, yellow-c and yellow-f genes are expressed at all developmental stages, especially in the prepupal stage. Using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, we successfully disrupted yellow-y, yellow-c and yellow-f in the BSF. Consequently, the mutation of yellow-y clearly resulted in a pale-yellow body color in prepupae, pupae and adults, instead of the typical black body color of the wild type. However, the mutation of yellow-c or yellow-f genes did not result in any change in color of the insects, when compared with the wild type. Our study indicates that the BSF yellow-y gene plays a role in body pigmentation, providing an optimal marker gene for the genetic manipulation of BSF.
Language	English
Publishing date	2024-04-29
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2179775-4
ISSN	1744-7917 ; 1672-9609
ISSN (online)	1744-7917
ISSN	1672-9609
DOI	10.1111/1744-7917.13371
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