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Book ; Thesis: Realtime simulation of stiff threads for microsurgery training simulation

Hüsken, Nathan

2014

Author's details	[by Nathan Hüsken]
Language	English
Size	XI, 138 S., Ill., graph. Darst.
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., Diss.--Heidelberg, 2014
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Book ; Online ; Thesis: Ferrocene-PNA recognition layers

Hüsken, Nina

probe design, interfacial and electron transfer studies and DNA detection strategies

2012

Author's details	presented by Nina Hüsken
Language	English
Size	Online-Ressource (PDF-Datei: 278 S., 8009 KB)
Publisher	Univ.-Bibliothek
Publishing place	Bochum
Document type	Book ; Online ; Thesis
Thesis / German Habilitation thesis	Univ., Diss.--Bochum, 2010
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article: MicroSim - a microsurgical training simulator.

Hüsken, Nathan / Schuppe, Oliver / Sismanidis, Evangelos / Beier, Florian

Studies in health technology and informatics

2013 Volume 184, Page(s) 205–209

Abstract: MicroSim is a training simulator for microsurgical tasks. It is based on a virtual reality environment including a realistic interface and a real-time simulation model. The interface consists of real instruments which are tracked by an optical tracking ... ...

Abstract	MicroSim is a training simulator for microsurgical tasks. It is based on a virtual reality environment including a realistic interface and a real-time simulation model. The interface consists of real instruments which are tracked by an optical tracking system. The surgical scene is presented to the user through a stereo display which is similar to the view a surgeon has through a microscope. Abstract training modules, which are used to train basic fine motor skills and the prototype of a microvascular anastomosis have been implemented in cooperation with VRmagic GmbH.
MeSH term(s)	Computer-Assisted Instruction/instrumentation ; Equipment Design ; Equipment Failure Analysis ; Humans ; Imaging, Three-Dimensional/instrumentation ; Microsurgery/education ; Microsurgery/instrumentation ; Surgery, Computer-Assisted/instrumentation ; User-Computer Interface
Language	English
Publishing date	2013
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	0926-9630
ISSN	0926-9630
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A single-electrode, dual-potential ferrocene-PNA biosensor for the detection of DNA.

Hüsken, Nina / Gebala, Magdalena / Schuhmann, Wolfgang / Metzler-Nolte, Nils

Chembiochem : a European journal of chemical biology

2010 Volume 11, Issue 12, Page(s) 1754–1761

Abstract: A Fc-PNA biosensor (Fc: ferrocenyl, C(10)H(9)Fe) was designed by using two electrochemically distinguishable recognition elements with different molecular information at a single electrode. Two Fc-PNA capture probes were therefore synthesized by N- ... ...

Abstract	A Fc-PNA biosensor (Fc: ferrocenyl, C(10)H(9)Fe) was designed by using two electrochemically distinguishable recognition elements with different molecular information at a single electrode. Two Fc-PNA capture probes were therefore synthesized by N-terminal labeling different dodecamer PNA sequences with different ferrocene derivatives by click chemistry. Each of the two strands was thereby tethered with one specific ferrocene derivative. The two capture probes revealed quasi-reversible redox processes of the Fc(0/+) redox couple with a significant difference in their electrochemical half-wave potentials of Delta E(1/2)=160 mV. A carefully designed biosensor interface, consisting of a ternary self-assembled monolayer (SAM) of the two C-terminal cysteine-tethered Fc-PNA capture probes and 6-mercaptohexanol, was electrochemically investigated by square wave (SWV) and cyclic voltammetry (CV). The biosensor properties of this interface were analyzed by studying the interaction with DNA sequences that were complementary to either of the two capture probes by SWV. Based on distinct changes in both peak current and potential, a parallel identification of these two DNA sequences was successful with one interface design. Moreover, the primary electrochemical response could be converted by a simple mathematical analysis into a clear-cut electrochemical signal about the hybridization event. The discrimination of single-nucleotide polymorphism (SNP) was proven with a chosen single-mismatch DNA sequence. Furthermore, experiments with crude bacterial RNA confirm the principal suitability of this dual-potential sensor under real-life conditions.
MeSH term(s)	Biosensing Techniques/methods ; Click Chemistry/methods ; DNA/analysis ; Ferrous Compounds/chemistry ; Metallocenes ; Peptide Nucleic Acids/chemistry ; Polymorphism, Single Nucleotide ; Potentiometry/methods
Chemical Substances	Ferrous Compounds ; Metallocenes ; Peptide Nucleic Acids ; DNA (9007-49-2) ; ferrocene (U96PKG90JQ)
Language	English
Publishing date	2010-08-16
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2020469-3
ISSN	1439-7633 ; 1439-4227
ISSN (online)	1439-7633
ISSN	1439-4227
DOI	10.1002/cbic.200900748
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Article ; Online: Impact of single basepair mismatches on electron-transfer processes at Fc-PNA⋅DNA modified gold surfaces.

Hüsken, Nina / Gębala, Magdalena / Battistel, Alberto / La Mantia, Fabio / Schuhmann, Wolfgang / Metzler-Nolte, Nils

Chemphyschem : a European journal of chemical physics and physical chemistry

2012 Volume 13, Issue 1, Page(s) 131–139

Abstract: Gold-surface grafted peptide nucleic acid (PNA) strands, which carry a redox-active ferrocene tag, present unique tools to electrochemically investigate their mechanical bending elasticity based on the kinetics of electron-transfer (ET) processes. A ... ...

Abstract	Gold-surface grafted peptide nucleic acid (PNA) strands, which carry a redox-active ferrocene tag, present unique tools to electrochemically investigate their mechanical bending elasticity based on the kinetics of electron-transfer (ET) processes. A comparative study of the mechanical bending properties and the thermodynamic stability of a series of 12-mer Fc-PNA⋅DNA duplexes was carried out. A single basepair mismatch was integrated at all possible strand positions to provide nanoscopic insights into the physicochemical changes provoked by the presence of a single basepair mismatch with regard to its position within the strand. The ET processes at single mismatch Fc-PNA⋅DNA modified surfaces were found to proceed with increasing diffusion limitation and decreasing standard ET rate constants k(0) when the single basepair mismatch was dislocated along the strand towards its free-dangling Fc-modified end. The observed ET characteristics are considered to be due to a punctual increase in the strand elasticity at the mismatch position. The kinetic mismatch discrimination with respect to the fully-complementary duplex presents a basis for an electrochemical DNA sensing strategy based on the Fc-PNA⋅DNA bending dynamics for loosely packed monolayers. In a general sense, the strand elasticity presents a further physicochemical property which is affected by a single basepair mismatch which may possibly be used as a basis for future DNA sensing concepts for the specific detection of single basepair mismatches.
MeSH term(s)	Base Pair Mismatch ; Base Sequence ; Biosensing Techniques ; DNA/chemistry ; Electrochemical Techniques ; Electrodes ; Electron Transport ; Gold/chemistry ; Kinetics ; Nucleic Acid Hybridization ; Oxidation-Reduction ; Peptide Nucleic Acids/chemistry ; Phase Transition ; Surface Properties
Chemical Substances	Peptide Nucleic Acids ; Gold (7440-57-5) ; DNA (9007-49-2)
Language	English
Publishing date	2012-01-16
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1439-7641
ISSN (online)	1439-7641
DOI	10.1002/cphc.201100578
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Article ; Online: A ruthenocene-PNA bioconjugate--synthesis, characterization, cytotoxicity, and AAS-detected cellular uptake.

Gross, Annika / Hüsken, Nina / Schur, Julia / Raszeja, Łukasz / Ott, Ingo / Metzler-Nolte, Nils

Bioconjugate chemistry

2012 Volume 23, Issue 9, Page(s) 1764–1774

Abstract: Labeling of peptide nucleic acids (PNA) with metallocene complexes is explored herein for the modulation of the analytical characteristics, as well as biological properties of PNA. The synthesis of the first ruthenocene-PNA conjugate with a dodecamer, ... ...

Abstract	Labeling of peptide nucleic acids (PNA) with metallocene complexes is explored herein for the modulation of the analytical characteristics, as well as biological properties of PNA. The synthesis of the first ruthenocene-PNA conjugate with a dodecamer, mixed-sequence PNA is described, and its properties are compared to a ferrocene-labeled analogue as well as an acetylated, metal-free derivative. The synthetic characteristics, chemical stability, analytical and thermodynamic properties, and the interaction with cDNA were investigated. Furthermore, the cytotoxicity of the PNA conjugates is determined on HeLa, HepG2, and PT45 cell lines. Finally, the cellular uptake of the metal-containing PNAs was quantified by high-resolution continuum source atomic absorption spectrometry (HR-CS AAS). An unexpectedly high cellular uptake to final concentrations of 4.2 mM was observed upon incubation with 50 μM solutions of the ruthenocene-PNA conjugate. The ruthenocene label was shown to be an excellent label in all respects, which is also more stable than its ferrocene analogue. Because of its high stability, low toxicity, and the lack of a natural background of ruthenium, it is an ideal choice for bioanalytical purposes and possible medicinal and biological applications like, e.g., the development of gene-targeted drugs.
MeSH term(s)	Cell Line, Tumor ; Chromatography, High Pressure Liquid ; Humans ; Organometallic Compounds/chemical synthesis ; Organometallic Compounds/chemistry ; Peptide Nucleic Acids/chemical synthesis ; Peptide Nucleic Acids/chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Spectrophotometry, Atomic/methods
Chemical Substances	Organometallic Compounds ; Peptide Nucleic Acids ; ruthenocene (1287-13-4)
Language	English
Publishing date	2012-09-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1024041-x
ISSN	1520-4812 ; 1043-1802
ISSN (online)	1520-4812
ISSN	1043-1802
DOI	10.1021/bc200692g
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Article ; Online: "Four-potential" ferrocene labeling of PNA oligomers via click chemistry.

Hüsken, Nina / Gasser, Gilles / Köster, S David / Metzler-Nolte, Nils

Bioconjugate chemistry

2009 Volume 20, Issue 8, Page(s) 1578–1586

Abstract: The scope of the Cu(I)-catalyzed [2 + 3] azide/alkyne cycloaddition (CuAAC, click chemistry) as a key reaction for the conjugation of ferrocene derivatives to N-terminal functionalized PNA oligomers is explored herein (PNA: peptide nucleic acid). The ... ...

Abstract	The scope of the Cu(I)-catalyzed [2 + 3] azide/alkyne cycloaddition (CuAAC, click chemistry) as a key reaction for the conjugation of ferrocene derivatives to N-terminal functionalized PNA oligomers is explored herein (PNA: peptide nucleic acid). The facile solid-phase synthesis of N-terminal azide or alkyne-functionalized PNA oligomer precursors and their cycloaddition with azidoferrocene, ethynylferrocene, and N-(3-ethylpent-1-yn-3-yl)ferrocene-carboxamide (DEPA-ferrocene) on the solid phase are presented. While the click reaction with azidomethylferrocene worked equally well, the ferrocenylmethyl group is lost from the conjugate upon acid cleavage. However, the desired product was obtained via a post-SPPS conversion of the alkyne-PNA oligomer with azidomethylferrocene in solution. The synthesis of all ferrocene-PNA conjugates (trimer t(3)-PNA, 3, 4, 5, 6; 12mer PNA, 10 - t c t a c a a g a c t c, 11 - t c t a c c g t a c t c) succeeded with excellent yields and purities, as determined by mass spectrometry and HPLC. Electrochemical studies of the trimer Fc-PNA conjugates 3, 4, 5, and 6 with four different ferrocene moieties revealed quasi-reversible redox processes of the ferrocenyl redox couple Fc(0/+) and electrochemical half-wave potentials in a range of E(1/2) = -20 mV to +270 mV vs FcH(0/+) (Fc: ferrocenyl, C(10)H(9)Fe). The observed potential differences ΔE(1/2)(min) are always greater than 60 mV for any given pair of Fc-PNA conjugates, thus allowing a reliable differentiation with sensitive electrochemical methods like e.g. square wave voltammetry (SWV). This is the electrochemical equivalent of "four-color" detection and is hence denoted "four-potential" labeling. Preparation and electrochemical investigation of the set of four structurally different and electrochemically distinguishable ferrocenyl groups conjugated to PNA oligomers, as exemplified by the conjugates 3, 4, 5, and 6, demonstrates the scope of the azide/alkyne cycloaddition for the labeling of PNA with electrochemically active ferrocenyl groups. Furthermore, it provides a PNA-based system for the electrochemical detection of single-nucleotide polymorphism (SNP) in DNA/RNA.
MeSH term(s)	Catalysis ; Click Chemistry/methods ; Copper/chemistry ; Cyclization ; Electrochemistry ; Ferrous Compounds/chemistry ; Metallocenes ; Molecular Structure ; Peptide Nucleic Acids/chemistry ; Staining and Labeling/methods ; Stereoisomerism
Chemical Substances	Ferrous Compounds ; Metallocenes ; Peptide Nucleic Acids ; Copper (789U1901C5) ; ferrocene (U96PKG90JQ)
Language	English
Publishing date	2009-08-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1024041-x
ISSN	1520-4812 ; 1043-1802
ISSN (online)	1520-4812
ISSN	1043-1802
DOI	10.1021/bc9001272
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Article ; Online: Mechanistic studies of Fc-PNA(⋅DNA) surface dynamics based on the kinetics of electron-transfer processes.

Hüsken, Nina / Gębala, Magdalena / La Mantia, Fabio / Schuhmann, Wolfgang / Metzler-Nolte, Nils

Chemistry (Weinheim an der Bergstrasse, Germany)

2011 Volume 17, Issue 35, Page(s) 9678–9690

Abstract: N-Terminally ferrocenylated and C-terminally gold-surface-grafted peptide nucleic acid (PNA) strands were exploited as unique tools for the electrochemical investigation of the strand dynamics of short PNA(⋅DNA) duplexes. On the basis of the quantitative ...

Abstract	N-Terminally ferrocenylated and C-terminally gold-surface-grafted peptide nucleic acid (PNA) strands were exploited as unique tools for the electrochemical investigation of the strand dynamics of short PNA(⋅DNA) duplexes. On the basis of the quantitative analysis of the kinetics and the diffusional characteristics of the electron-transfer process, a nanoscopic view of the Fc-PNA(⋅DNA) surface dynamics was obtained. Loosely packed, surface-confined Fc-PNA single strands were found to render the charge-transfer process of the tethered Fc moiety diffusion-limited, whereas surfaces modified with Fc-PNA⋅DNA duplexes exhibited a charge-transfer process with characteristics between the two extremes of diffusion and surface limitation. The interplay between the inherent strand elasticity and effects exerted by the electric field are supposed to dictate the probability of a sufficient approach of the Fc head group to the electrode surface, as reflected in the measured values of the electron-transfer rate constant, k(0). An in-depth understanding of the dynamics of surface-bound PNA and PNA⋅DNA strands is of utmost importance for the development of DNA biosensors using (Fc-)PNA recognition layers.
MeSH term(s)	Base Sequence ; Biosensing Techniques ; DNA/analysis ; DNA/chemistry ; DNA/metabolism ; DNA, Single-Stranded/chemistry ; Diffusion ; Electrochemistry ; Electron Transport ; Ferrous Compounds/chemistry ; Gold/chemistry ; Kinetics ; Models, Chemical ; Nucleic Acid Conformation ; Peptide Nucleic Acids/chemistry ; Peptide Nucleic Acids/metabolism ; Thermodynamics
Chemical Substances	DNA, Single-Stranded ; Ferrous Compounds ; Peptide Nucleic Acids ; Gold (7440-57-5) ; DNA (9007-49-2)
Language	English
Publishing date	2011-08-22
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1478547-x
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.201003764
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Article ; Online: Bulk protonic conductivity in a cephalopod structural protein.

Ordinario, David D / Phan, Long / Walkup, Ward G / Jocson, Jonah-Micah / Karshalev, Emil / Hüsken, Nina / Gorodetsky, Alon A

Nature chemistry

2014 Volume 6, Issue 7, Page(s) 596–602

Abstract: Proton-conducting materials play a central role in many renewable energy and bioelectronics technologies, including fuel cells, batteries and sensors. Thus, much research effort has been expended to develop improved proton-conducting materials, such as ... ...

Abstract	Proton-conducting materials play a central role in many renewable energy and bioelectronics technologies, including fuel cells, batteries and sensors. Thus, much research effort has been expended to develop improved proton-conducting materials, such as ceramic oxides, solid acids, polymers and metal-organic frameworks. Within this context, bulk proton conductors from naturally occurring proteins have received somewhat less attention than other materials, which is surprising given the potential modularity, tunability and processability of protein-based materials. Here, we report proton conductivity for thin films composed of reflectin, a cephalopod structural protein. Bulk reflectin has a proton conductivity of ~2.6 × 10(-3) S cm(-1) at 65 °C, a proton transport activation energy of ~0.2 eV and a proton mobility of ~7 × 10(-3) cm(2) V(-1) s(-1). These figures of merit are similar to those reported for state-of-the-art artificial proton conductors and make it possible to use reflectin in protein-based protonic transistors. Our findings may hold implications for the next generation of biocompatible proton-conducting materials and protonic devices.
MeSH term(s)	Animals ; Cephalopoda/chemistry ; Polymers/chemistry ; Proton Therapy
Chemical Substances	Polymers
Language	English
Publishing date	2014-06-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2464596-5
ISSN	1755-4349 ; 1755-4330
ISSN (online)	1755-4349
ISSN	1755-4330
DOI	10.1038/nchem.1960
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Article ; Online: Synthesis of organometallic PNA oligomers by click chemistry.

Gasser, Gilles / Hüsken, Nina / Köster, S David / Metzler-Nolte, Nils

Chemical communications (Cambridge, England)

2008 , Issue 31, Page(s) 3675–3677

Abstract: The facile side-specific insertion, on the solid phase, of one or two ferrocene moieties into peptide nucleic acid (PNA) oligomers by click chemistry is presented. ...

Abstract	The facile side-specific insertion, on the solid phase, of one or two ferrocene moieties into peptide nucleic acid (PNA) oligomers by click chemistry is presented.
MeSH term(s)	Base Sequence ; Ferrous Compounds/chemistry ; Metallocenes ; Organometallic Compounds/chemical synthesis ; Organometallic Compounds/chemistry ; Peptide Nucleic Acids/chemical synthesis ; Peptide Nucleic Acids/chemistry
Chemical Substances	Ferrous Compounds ; Metallocenes ; Organometallic Compounds ; Peptide Nucleic Acids ; ferrocene (U96PKG90JQ)
Language	English
Publishing date	2008-08-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/b805369c
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