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Article ; Online: mSphere of Influence: Tweaking Organellar Purification Approaches.

Huet, Diego

mSphere

2020 Volume 5, Issue 5

Abstract: Diego Huet studies the organelles involved in the metabolic adaptations of the apicomplexan ... ...

Abstract	Diego Huet studies the organelles involved in the metabolic adaptations of the apicomplexan parasite
MeSH term(s)	Cytological Techniques ; Humans ; Metabolome ; Mitochondria/metabolism ; Toxoplasma/metabolism
Language	English
Publishing date	2020-09-09
Publishing country	United States
Document type	Journal Article
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/mSphere.00690-20
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Article ; Online: Interorganellar Communication Through Membrane Contact Sites in

Huet, Diego / Moreno, Silvia N J

Contact (Thousand Oaks (Ventura County, Calif.))

2023 Volume 6, Page(s) 25152564231189064

Abstract: Apicomplexan parasites are a group of protists that cause disease in humans and include pathogens ... ...

Abstract	Apicomplexan parasites are a group of protists that cause disease in humans and include pathogens like
Language	English
Publishing date	2023-08-06
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2964312-0
ISSN	2515-2564 ; 2515-2564
ISSN (online)	2515-2564
ISSN	2515-2564
DOI	10.1177/25152564231189064
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Article ; Online: ATP synthase-associated coiled-coil-helix-coiled-coil-helix (CHCH) domain-containing proteins are critical for mitochondrial function in

Usey, Madelaine M / Huet, Diego

mBio

2023 Volume 14, Issue 5, Page(s) e0176923

Abstract: Importance: Members of the coiled-coil-helix-coiled-coil-helix (CHCH) domain protein family are transported into the mitochondrial intermembrane space, where they play important roles in the biogenesis and function of the organelle. Unexpectedly, the ... ...

Abstract	Importance: Members of the coiled-coil-helix-coiled-coil-helix (CHCH) domain protein family are transported into the mitochondrial intermembrane space, where they play important roles in the biogenesis and function of the organelle. Unexpectedly, the ATP synthase of the apicomplexan
MeSH term(s)	Animals ; Toxoplasma/genetics ; Toxoplasma/metabolism ; Mitochondria/metabolism ; Proteins/metabolism ; Parasites/metabolism ; Nitric Oxide Synthase/metabolism ; Adenosine Triphosphate/metabolism ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; Mitochondrial Proteins/metabolism
Chemical Substances	Proteins ; Nitric Oxide Synthase (EC 1.14.13.39) ; Adenosine Triphosphate (8L70Q75FXE) ; Protozoan Proteins ; Mitochondrial Proteins
Language	English
Publishing date	2023-10-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.01769-23
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Article ; Online: Parasite powerhouse: A review of the Toxoplasma gondii mitochondrion.

Usey, Madelaine M / Huet, Diego

The Journal of eukaryotic microbiology

2022 Volume 69, Issue 6, Page(s) e12906

Abstract: Toxoplasma gondii is a member of the apicomplexan phylum, a group of single-celled eukaryotic parasites that cause significant human morbidity and mortality around the world. T. gondii harbors two organelles of endosymbiotic origin: a non-photosynthetic ... ...

Abstract	Toxoplasma gondii is a member of the apicomplexan phylum, a group of single-celled eukaryotic parasites that cause significant human morbidity and mortality around the world. T. gondii harbors two organelles of endosymbiotic origin: a non-photosynthetic plastid, known as the apicoplast, and a single mitochondrion derived from the ancient engulfment of an α-proteobacterium. Due to excitement surrounding the novelty of the apicoplast, the T. gondii mitochondrion was, to a certain extent, overlooked for about two decades. However, recent work has illustrated that the mitochondrion is an essential hub of apicomplexan-specific biology. Development of novel techniques, such as cryo-electron microscopy, complexome profiling, and next-generation sequencing have led to a renaissance in mitochondrial studies. This review will cover what is currently known about key features of the T. gondii mitochondrion, ranging from its genome to protein import machinery and biochemical pathways. Particular focus will be given to mitochondrial features that diverge significantly from the mammalian host, along with discussion of this important organelle as a drug target.
MeSH term(s)	Animals ; Humans ; Toxoplasma/metabolism ; Parasites ; Cryoelectron Microscopy ; Apicoplasts/metabolism ; Mitochondria/metabolism ; Mammals
Language	English
Publishing date	2022-05-04
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1147218-2
ISSN	1550-7408 ; 1066-5234
ISSN (online)	1550-7408
ISSN	1066-5234
DOI	10.1111/jeu.12906
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Article ; Online: Editorial: Disruptive technologies for the study of host-pathogen interactions.

Quintana, Juan F / Harding, Clare / Huet, Diego / Sateriale, Adam / Bernabeu, Maria

Frontiers in cellular and infection microbiology

2022 Volume 12, Page(s) 1048342

MeSH term(s)	Disruptive Technology ; Host-Pathogen Interactions ; Host-Parasite Interactions
Language	English
Publishing date	2022-10-13
Publishing country	Switzerland
Document type	Editorial ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2022.1048342
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Article ; Online: The mystery of massive mitochondrial complexes: the apicomplexan respiratory chain.

Maclean, Andrew E / Hayward, Jenni A / Huet, Diego / van Dooren, Giel G / Sheiner, Lilach

Trends in parasitology

2022 Volume 38, Issue 12, Page(s) 1041–1052

Abstract: The mitochondrial respiratory chain is an essential pathway in most studied eukaryotes due to its roles in respiration and other pathways that depend on mitochondrial membrane potential. Apicomplexans are unicellular eukaryotes whose members have an ... ...

Abstract	The mitochondrial respiratory chain is an essential pathway in most studied eukaryotes due to its roles in respiration and other pathways that depend on mitochondrial membrane potential. Apicomplexans are unicellular eukaryotes whose members have an impact on global health. The respiratory chain is a drug target for some members of this group, notably the malaria-causing Plasmodium spp. This has motivated studies of the respiratory chain in apicomplexan parasites, primarily Toxoplasma gondii and Plasmodium spp. for which experimental tools are most advanced. Studies of the respiratory complexes in these organisms revealed numerous novel features, including expansion of complex size. The divergence of apicomplexan mitochondria from commonly studied models highlights the diversity of mitochondrial form and function across eukaryotic life.
MeSH term(s)	Humans ; Electron Transport ; Mitochondria/metabolism ; Toxoplasma ; Plasmodium/metabolism ; Malaria/parasitology ; Apicomplexa/metabolism
Language	English
Publishing date	2022-10-24
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2036227-4
ISSN	1471-5007 ; 1471-4922
ISSN (online)	1471-5007
ISSN	1471-4922
DOI	10.1016/j.pt.2022.09.008
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Article ; Online: IFT25 is required for the construction of the trypanosome flagellum.

Huet, Diego / Blisnick, Thierry / Perrot, Sylvie / Bastin, Philippe

Journal of cell science

2019 Volume 132, Issue 5

Abstract: Intraflagellar transport (IFT), the movement of protein complexes responsible for the assembly of cilia and flagella, is remarkably conserved from protists to humans. However, two IFT components (IFT25 and IFT27) are missing from multiple unrelated ... ...

Abstract	Intraflagellar transport (IFT), the movement of protein complexes responsible for the assembly of cilia and flagella, is remarkably conserved from protists to humans. However, two IFT components (IFT25 and IFT27) are missing from multiple unrelated eukaryotic species. In mouse, IFT25 (also known as HSPB11) and IFT27 are not required for assembly of several cilia with the noticeable exception of the flagellum of spermatozoa. Here, we show that the
MeSH term(s)	Animals ; Cilia/metabolism ; Flagella/genetics ; Flagella/metabolism ; Intracellular Signaling Peptides and Proteins/genetics ; Intracellular Signaling Peptides and Proteins/metabolism ; Male ; Mice ; Molecular Chaperones/genetics ; Molecular Chaperones/metabolism ; Protein Transport ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; Spermatozoa/metabolism ; Trypanosoma brucei brucei/physiology ; rab GTP-Binding Proteins/genetics ; rab GTP-Binding Proteins/metabolism
Chemical Substances	IFT25 protein, mouse ; Intracellular Signaling Peptides and Proteins ; Molecular Chaperones ; Protozoan Proteins ; intraflagellar transport 27 protein, mouse ; rab GTP-Binding Proteins (EC 3.6.5.2)
Language	English
Publishing date	2019-02-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2993-2
ISSN	1477-9137 ; 0021-9533
ISSN (online)	1477-9137
ISSN	0021-9533
DOI	10.1242/jcs.228296
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Article ; Online: CRISPR-Cas9-based genome-wide screening of Toxoplasma gondii.

Sidik, Saima M / Huet, Diego / Lourido, Sebastian

Nature protocols

2018 Volume 13, Issue 1, Page(s) 307–323

Abstract: Apicomplexan parasites, such as Toxoplasma gondii, cause extensive morbidity and mortality in humans and livestock, highlighting the need for a deeper understanding of their molecular biology. Although techniques for the generation of targeted gene ... ...

Abstract	Apicomplexan parasites, such as Toxoplasma gondii, cause extensive morbidity and mortality in humans and livestock, highlighting the need for a deeper understanding of their molecular biology. Although techniques for the generation of targeted gene disruptions have long been available for apicomplexans, such methods are not readily scalable to the entire genome. We recently used CRISPR-Cas9 to disrupt all nuclear protein-coding genes in T. gondii using a pooled format. The method relies on transfection of a guide RNA library into parasites constitutively expressing Cas9. Here, we present the complete workflow of such a screen, including preparation of the guide RNA library, growth and testing of the recipient strain, generation of the mutant population, culture conditions for the screen, preparation of genomic DNA libraries, next-generation sequencing of the guide RNA loci, and analysis to detect fitness-conferring genes. This method can be deployed to study how culture conditions affect the repertoire of genes needed by parasites, which will enable studies of their metabolic needs, host specificity, and drug-resistance mechanisms. In addition, by manipulating the background in which the screen is performed, researchers will be able to investigate genetic interactions, which may help uncover redundancy or epistasis in the parasite genome. Using this method, a genome-wide screen and its analysis can be completed in 3 weeks, after ∼1 month of preparation to generate the library and grow the cells needed, making it a powerful tool for uncovering functionally important genes in apicomplexan parasites.
MeSH term(s)	CRISPR-Cas Systems/physiology ; Chromosome Mapping/methods ; Clustered Regularly Interspaced Short Palindromic Repeats ; Gene Knockout Techniques/methods ; Genome, Protozoan ; Genomic Library ; Humans ; Nuclear Proteins/genetics ; Nuclear Proteins/physiology ; RNA, Guide, CRISPR-Cas Systems ; Toxoplasma/genetics ; Transfection
Chemical Substances	Nuclear Proteins ; RNA, Guide, CRISPR-Cas Systems
Language	English
Publishing date	2018-01-11
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2244966-8
ISSN	1750-2799 ; 1754-2189
ISSN (online)	1750-2799
ISSN	1754-2189
DOI	10.1038/nprot.2017.131
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Article ; Online: Review of basal-plus insulin regimen options for simpler insulin intensification in people with Type 2 diabetes mellitus.

Raccah, D / Huet, D / Dib, A / Joseph, F / Landers, B / Escalada, J / Schmitt, H

Diabetic medicine : a journal of the British Diabetic Association

2017 Volume 34, Issue 9, Page(s) 1193–1204

Abstract: Aims: To identify simple insulin regimens for people with Type 2 diabetes mellitus that can be accepted and implemented earlier in primary and specialist care, taking into consideration each individual's needs and capabilities.: Methods: Using ... ...

Abstract	Aims: To identify simple insulin regimens for people with Type 2 diabetes mellitus that can be accepted and implemented earlier in primary and specialist care, taking into consideration each individual's needs and capabilities. Methods: Using randomized clinical trials identified by a search of the PubMed database, as well as systematic reviews, meta-analyses and proof-of-concept studies, this review addresses topics of interest related to the progressive intensification of a basal insulin regimen to a basal-plus regimen (one basal insulin injection plus stepwise addition of one to three preprandial short-acting insulin injections/day) vs a basal-bolus regimen (basal insulin plus three short-acting insulin injections per day) in people with Type 2 diabetes. The review explores approaches that can be used to define the meal for first prandial injection with basal-plus regimens, differences among insulin titration algorithms, and the importance of self-motivation and autonomy in achieving optimum glycaemic control. Results: A basal-plus regimen can provide glycaemic control equivalent to that obtained with a full basal-bolus regimen, with fewer injections of prandial insulin. The first critical step is to optimize basal insulin dosing to reach a fasting glucose concentration of ~6.7 mmol/l; this allows ~40% of patients with baseline HbA Conclusions: Compared with a basal-bolus regimen, a basal-plus insulin regimen is as effective but more practical, and has the best chance of acceptance and success in the real world.
MeSH term(s)	Blood Glucose/analysis ; Blood Glucose/drug effects ; Blood Glucose/metabolism ; Diabetes Mellitus, Type 2/blood ; Diabetes Mellitus, Type 2/drug therapy ; Diabetes Mellitus, Type 2/epidemiology ; Dose-Response Relationship, Drug ; Drug Administration Schedule ; Drug Therapy, Combination/methods ; Glycated Hemoglobin A/analysis ; Glycated Hemoglobin A/metabolism ; Humans ; Hypoglycemic Agents/administration & dosage ; Hypoglycemic Agents/adverse effects ; Insulin/administration & dosage ; Insulin/adverse effects
Chemical Substances	Blood Glucose ; Glycated Hemoglobin A ; Hypoglycemic Agents ; Insulin
Language	English
Publishing date	2017-07-09
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	605769-x
ISSN	1464-5491 ; 0742-3071 ; 1466-5468
ISSN (online)	1464-5491
ISSN	0742-3071 ; 1466-5468
DOI	10.1111/dme.13390
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Article ; Online: Infection pulmonaire à Mycobacterium malmoense. Difficultés diagnostiques et de thérapeutiques.

Huet, D / Godbert, B / Hermann, J / Zordan, J-M / Chabot, F / Andréjak, C

Revue des maladies respiratoires

2017 Volume 34, Issue 3, Page(s) 257–261

Abstract: Introduction: Pulmonary infection due to Mycobacterium malmoense can be difficult to diagnose. These difficulties can be responsible for a delay in the implementation of optimal treatment. Moreover, the treatment is not standardized.: Observation: We ...

Title translation	Pulmonary infection with Mycobacterium malmoense. Difficulties in diagnosis and treatment.
Abstract	Introduction: Pulmonary infection due to Mycobacterium malmoense can be difficult to diagnose. These difficulties can be responsible for a delay in the implementation of optimal treatment. Moreover, the treatment is not standardized. Observation: We report the case of a 56-year-old patient who developed a Mycobacterium malmoense pulmonary infection whose diagnosis was delayed due to initial suspicion of pulmonary Mycobacterium tuberculosis infection. Once the diagnosis was confirmed, the patient was treated empirically with rifampicin, ethambutol, and clarithromycin for 12 months after culture conversion, giving a total of 15 months. The clinical and radiological outcomes were favorable. Discussion: This clinical case highlights the difficulties of diagnosing pulmonary atypical mycobacterial infection according to the American Thoracic Society criteria, particularly Mycobacterium malmoense, a non-tuberculous mycobacterium (NTM) quite uncommon in France. Currently, there are new diagnostic techniques such as GenoType Mycobacteria Direct Conclusion: A more systematic reporting strategy could allow cohort studies and therefore provide us with data on the most efficient drugs in the treatment of the rarest NTM infections.
MeSH term(s)	Clarithromycin/administration & dosage ; Diagnosis, Differential ; Ethambutol/administration & dosage ; Humans ; Lung Diseases/diagnosis ; Lung Diseases/drug therapy ; Lung Diseases/microbiology ; Male ; Middle Aged ; Mycobacterium Infections, Nontuberculous/diagnosis ; Mycobacterium Infections, Nontuberculous/drug therapy ; Mycobacterium Infections, Nontuberculous/microbiology ; Nontuberculous Mycobacteria/isolation & purification ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/drug therapy ; Rifampin/administration & dosage ; Tuberculosis, Pulmonary/diagnosis
Chemical Substances	Ethambutol (8G167061QZ) ; Clarithromycin (H1250JIK0A) ; Rifampin (VJT6J7R4TR)
Language	French
Publishing date	2017-03
Publishing country	France
Document type	Case Reports ; Journal Article
ZDB-ID	605743-3
ISSN	1776-2588 ; 0301-0279 ; 0761-8425
ISSN (online)	1776-2588
ISSN	0301-0279 ; 0761-8425
DOI	10.1016/j.rmr.2016.05.012
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