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Article ; Online: Multimodal neural probes for combined optogenetics and electrophysiology

Huihui Tian / Ke Xu / Liang Zou / Ying Fang

iScience, Vol 25, Iss 1, Pp 103612- (2022)

2022

Abstract: Summary: To understand how brain functions arise from interconnected neural networks, it is necessary to develop tools that can allow simultaneous manipulation and recording of neural activities. Multimodal neural probes, especially those that combine ... ...

Abstract	Summary: To understand how brain functions arise from interconnected neural networks, it is necessary to develop tools that can allow simultaneous manipulation and recording of neural activities. Multimodal neural probes, especially those that combine optogenetics with electrophysiology, provide a powerful tool for the dissection of neural circuit functions and understanding of brain diseases. In this review, we provide an overview of recent developments in multimodal neural probes. We will focus on materials and integration strategies of multimodal neural probes to achieve combined optogenetic stimulation and electrical recordings with high spatiotemporal precision and low invasiveness. In addition, we will also discuss future opportunities of multimodal neural interfaces in basic and translational neuroscience.
Keywords	Neuroscience ; Biotechnology ; Materials science ; Science ; Q
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The Viral Protein Poly(A) Polymerase Catalytic Subunit Interacts with Guanylate-Binding Proteins 2 to Antagonize the Antiviral Ability of Targeting Ectromelia Virus

Zhenzhen Gao / Xiaobing He / Guohua Chen / Yongxiang Fang / Zejing Meng / Huihui Tian / Hui Zhang / Zhizhong Jing

International Journal of Molecular Sciences, Vol 24, Iss 21, p

2023 Volume 15750

Abstract: The recent spread of the monkeypox virus among humans has heightened concerns regarding orthopoxvirus infections. Consequently, conducting a comprehensive study on the immunobiology of the monkeypox virus is imperative for the development of effective ... ...

Abstract	The recent spread of the monkeypox virus among humans has heightened concerns regarding orthopoxvirus infections. Consequently, conducting a comprehensive study on the immunobiology of the monkeypox virus is imperative for the development of effective therapeutics. Ectromelia virus (ECTV) closely resembles the genetic and disease characteristics of monkeypox virus, making it a valuable research tool for studying orthopoxvirus–host interactions. Guanylate-binding proteins (GBPs), highly expressed interferon-stimulated genes (ISGs), have antagonistic effects against various intracellular pathogenic microorganisms. Our previous research has shown that GBP2 has a mild but statistically significant inhibitory effect on ECTV infection. The presence of a significant number of molecules in the poxvirus genome that encode the host immune response raises questions about whether it also includes proteins that counteract the antiviral activity of GBP2. Using IP/MS and co-IP technology, we discovered that the poly(A) polymerase catalytic subunit (PAPL) protein of ECTV is a viral regulatory molecule that interacts with GBP2. Further studies have shown that PAPL antagonizes the antiviral activity of GBP2 by reducing its protein levels. Knocking out the PAPL gene of ECTV with the CRISPR/Cas9 system significantly diminishes the replication ability of the virus, indicating the indispensable role of PAPL in the replication process of ECTV. In conclusion, our study presents preliminary evidence supporting the significance of PAPL as a virulence factor that can interact with GBP2.
Keywords	ectromelia virus (ECTV) ; poly(A) polymerase catalytic subunit (PAPL) ; guanylate-binding protein (GBP) ; ISG ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	570
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Guanylate-Binding Protein 2 Exerts GTPase-Dependent Anti-Ectromelia Virus Effect

Zhenzhen Gao / Zejing Meng / Xiaobing He / Guohua Chen / Yongxiang Fang / Huihui Tian / Hui Zhang / Zhizhong Jing

Microorganisms, Vol 11, Iss 2258, p

2023 Volume 2258

Abstract: Guanylate-binding proteins (GBPs) are highly expressed interferon-stimulated genes (ISGs) that play significant roles in protecting against invading pathogens. Although their functions in response to RNA viruses have been extensively investigated, there ... ...

Abstract	Guanylate-binding proteins (GBPs) are highly expressed interferon-stimulated genes (ISGs) that play significant roles in protecting against invading pathogens. Although their functions in response to RNA viruses have been extensively investigated, there is limited information available regarding their role in DNA viruses, particularly poxviruses. Ectromelia virus (ECTV), a member of the orthopoxvirus genus, is a large double-stranded DNA virus closely related to the monkeypox virus and variola virus. It has been intensively studied as a highly effective model virus. According to the study, GBP2 overexpression suppresses ECTV replication in a dose-dependent manner, while GBP2 knockdown promotes ECTV infection. Additionally, it was discovered that GBP2 primarily functions through its N-terminal GTPase activity, and the inhibitory effect of GBP2 was disrupted in the GTP-binding-impaired mutant GBP2 K51A . This study is the first to demonstrate the inhibitory effect of GBP2 on ECTV, and it offers insights into innovative antiviral strategies.
Keywords	guanylate binding protein (GBP) ; IFN-stimulated genes (ISGs) ; ectromelia virus (ECTV) ; GTPase ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2023-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology

Liang Zou / Huihui Tian / Shouliang Guan / Jianfei Ding / Lei Gao / Jinfen Wang / Ying Fang

Nature Communications, Vol 12, Iss 1, Pp 1-

2021 Volume 9

Abstract: Abstract Optogenetics combined with electrical recording has emerged as a powerful tool for investigating causal relationships between neural circuit activity and function. However, the size of optogenetically manipulated tissue is typically 1-2 orders ... ...

Abstract	Abstract Optogenetics combined with electrical recording has emerged as a powerful tool for investigating causal relationships between neural circuit activity and function. However, the size of optogenetically manipulated tissue is typically 1-2 orders of magnitude larger than that can be electrically recorded, rendering difficulty for assigning functional roles of recorded neurons. Here we report a viral vector-delivery optrode (VVD-optrode) system for precise integration of optogenetics and electrophysiology in the brain. Our system consists of flexible microelectrode filaments and fiber optics that are simultaneously self-assembled in a nanoliter-scale, viral vector-delivery polymer carrier. The highly localized delivery and neuronal expression of opsin genes at microelectrode-tissue interfaces ensure high spatial congruence between optogenetically manipulated and electrically recorded neuronal populations. We demonstrate that this multifunctional system is capable of optogenetic manipulation and electrical recording of spatially defined neuronal populations for three months, allowing precise and long-term studies of neural circuit functions.
Keywords	Science ; Q
Subject code	612
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Quantification of Water Content during Freeze–Thaw Cycles

Huihui Tian / Changfu Wei / Yuanming Lai / Pan Chen

Vadose Zone Journal, Vol 17, Iss

A Nuclear Magnetic Resonance Based Method

2018 Volume 1

Abstract: In analyzing the phase transition and water migration processes in multiphase porous systems, it is helpful to properly evaluate the unfrozen capillary water content and adsorptive water content, respectively. In this study, an innovative procedure was ... ...

Abstract	In analyzing the phase transition and water migration processes in multiphase porous systems, it is helpful to properly evaluate the unfrozen capillary water content and adsorptive water content, respectively. In this study, an innovative procedure was developed to determine the total, adsorptive, and capillary water contents in frozen soils based on the nuclear magnetic resonance (NMR) technique. We found a threshold value of the proton spin–spin relaxation time, , that separates the unfrozen pore water into two distinct regimes: the adsorptive regime for small values and the capillary regime for large values. We showed that the relationship between adsorptive (or capillary) water content and temperature is hysteretic for all three tested soils experiencing a freeze–thaw cycle. The hysteresis behavior of capillary or adsorptive water content during a freeze–thaw cycle is attributable to capillary effect, metastable nucleation, pore blocking, and variation of microstructure. In all the tested soils, the adsorptive water content was significantly larger than the capillary water content and was supported by the experimental evidence inferred from previous pore water potential measurements of various soils.
Keywords	Environmental sciences ; GE1-350 ; Geology ; QE1-996.5
Language	English
Publishing date	2018-03-01T00:00:00Z
Publisher	Wiley
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Binary Thiolate DNA/Ferrocenyl Self-Assembled Monolayers on Gold: A Versatile Platform for Probing Biosensing Interfaces

Qi, Lin / Hua-Zhong Yu / Huihui Tian

Analytical chemistry. 2018 June 25, v. 90, no. 15

2018

Abstract: The properties of DNA self-assembled monolayers (SAMs) have strong influences on the interfacial DNA–analyte binding behavior, which further affect the performance of biosensors built upon. In this work, we prepared binary thiolate DNA/6-ferrocenyl-1- ... ...

Abstract	The properties of DNA self-assembled monolayers (SAMs) have strong influences on the interfacial DNA–analyte binding behavior, which further affect the performance of biosensors built upon. In this work, we prepared binary thiolate DNA/6-ferrocenyl-1-hexanethiol (FcC6SH) SAMs on gold (DNA/FcC6S-Au) for convenient electrochemical characterization and subsequent data analysis. Our cyclic voltammetric (CV) studies confirmed that the redox responses of surface-tethered Fc and electrostatically bound [Ru(NH3)6]3+ are capable of providing quantitative information regarding the DNA film properties, including the surface density, structural heterogeneity, and molecular orientation under different preparation and measurement conditions. With the binary thiolate DNA/FcC6S-Au SAM prepared in the conventional post-assembly exchange protocol as a trial system, we are demonstrating the capability of introducing redox-active thiols as passivating and labeling reagents for preparing many other DNA-based biosensing interfaces via varied assembly steps and under different measurement conditions.
Keywords	ammonia ; biosensors ; DNA ; electrochemistry ; gold ; thiols
Language	English
Dates of publication	2018-0625
Size	p. 9174-9181.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.8b01655
Database	NAL-Catalogue (AGRICOLA)

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Article: Straw strips mulch on furrows improves water use efficiency and yield of potato in a rainfed semiarid area

Chen, Yuzhang / Shouxi Chai / Huihui Tian / Yuwei Chai / Yawei Li / Lei Chang / Hongbo Cheng

Agricultural water management. 2019 Jan. 01, v. 211

2019

Abstract: Potato (Solanum tuberosum L.) production in semiarid regions is always constrained by water availability. This study was to evaluate the effects of straw strips mulch to potato growth, yield and economic benefits. The field experiment was conducted in ... ...

Abstract	Potato (Solanum tuberosum L.) production in semiarid regions is always constrained by water availability. This study was to evaluate the effects of straw strips mulch to potato growth, yield and economic benefits. The field experiment was conducted in northwest China in 2016 and 2017, including four treatments: (1), alternating narrow and wide ridges both mulched by black polyethylene film (PM); (2) alternating large ridges and small furrows with maize straw strips mulched only on the furrows (RS); (3) alternating the strips mulched with maize straw and bare plots with no ridges (PS); (4) traditional flat planting without mulching (CK). Results indicated that mulched treatments significantly (P < 0.05) increased tuber yield and WUE of potato compared with CK. The magnitude of yield in mulched treatments increase were 36.9–61.2% in 2016 and 38.7–45.5% in 2017, respectively, compared with CK. PM and RS treatments produced the highest tuber yield and enhanced water use efficiency by up to 74.8%, compared with CK. Soil water storage (0–2 m depth) was 21.5–39.9 mm in 2016 and 16.7–41.2 mm in 2017, respectively, greater in the mulched treatments, which significantly (P < 0.05) reduced evapotranspiration from sowing to seedling stages, compared with CK. Greater water availability was associated with greater biomass accumulation in above- and below-ground components, and plant height gain, during the growing season. PM significantly (P < 0.05) increased soil temperature (5–25 cm depth), which advanced the duration whole growth period by 6 days on average. RS and PM treatments experienced lower soil temperature (5–25 cm depth), which prolonged the duration whole growth period by 10 days on average, and increased the mass and percentage of Grade 1 tubers and the commodity rate (P < 0.05). As straw strips mulch application has lower input and labor costs than plastic film mulch, it produced the greatest economic benefits during this study, and it should be considered as an environmentally-friendly alternative to plastic film mulch for potato production in semiarid areas.
Keywords	Solanum tuberosum ; biomass production ; corn straw ; crop yield ; evapotranspiration ; field experimentation ; financial economics ; furrows ; growing season ; mulching ; plant height ; planting ; plastic film mulches ; polyethylene film ; potatoes ; seedlings ; semiarid zones ; soil temperature ; soil water ; soil water storage ; sowing ; tubers ; wages and remuneration ; water use efficiency ; China
Language	English
Dates of publication	2019-0101
Size	p. 142-151.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	751144-9
ISSN	1873-2283 ; 0378-3774
ISSN (online)	1873-2283
ISSN	0378-3774
DOI	10.1016/j.agwat.2018.09.048
Database	NAL-Catalogue (AGRICOLA)

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Article: Host–Guest Interaction at Molecular Interfaces: Cucurbit[7]uril as a Sensitive Probe of Structural Heterogeneity in Ferrocenyl Self-Assembled Monolayers on Gold

Qi, Lin / Hua-Zhong Yu / Huibo Shao / Huihui Tian

Journal of physical chemistry. 2018 Mar. 02, v. 122, no. 28

2018

Abstract: Herein, we combine host–guest recognition chemistry and electrochemical analysis to demonstrate that the nanometer-size, supramolecular hosts can be adapted as sensitive probes for structural heterogeneity in organized molecular assemblies on a surface. ... ...

Abstract	Herein, we combine host–guest recognition chemistry and electrochemical analysis to demonstrate that the nanometer-size, supramolecular hosts can be adapted as sensitive probes for structural heterogeneity in organized molecular assemblies on a surface. In particular, we carried out thorough cyclic voltammetric (CV) studies to evaluate the binding of cucurbit[7]uril on mixed ferrocenylundecanethiolate/n-alkanethiolate self-assembled monolayers (SAMs) on gold (FcC11S-/CmS-Au) prepared with different methods (coadsorption vs postassembly exchanges) and with varied diluting n-alkanethiols. On the basis of the distinct CV responses of CB[7]@Fc complex and free Fc on the SAM surfaces, we were able to determine the conversion ratio from Fc to CB[7]@Fc, a direct indication of its overall density and uniformity. We have shown that the FcC11S-/C8S-Au prepared by coadsorption in a binary solution with low mole fraction of FcC11SH (5%) and by exchanging preassembled C8S-Au SAM with FcC11SH for a short time (1 min) has the “ideal” structure with isolated and uniformly distributed Fc groups on the surface. In contrast, with similar Fc surface coverage, the FcC11S-/C8S-Au prepared by exchanging FcC11S-Au with C8SH for a prolonged time (20 h) has clustered and nonuniformly distributed Fc groups at the surface. While consistent with previous observations based on conventional electrochemical or microscopic studies, the present finding expands the capability of host–guest chemistry as a new tool to probe the structures of organized molecular assemblies at the nanometer scale.
Keywords	chemical bonding ; electrochemistry ; gold
Language	English
Dates of publication	2018-0302
Size	p. 15986-15995.
Publishing place	American Chemical Society
Document type	Article
ISSN	1932-7455
DOI	10.1021/acs.jpcc.8b01067
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Recurrence characteristics and clinicopathological results of borderline ovarian tumors

Lina Niu / Huihui Tian / Yongjun Xu / Jieqiong Cao / Xu Zhang / Junli Zhang / Jiajia Hou / Weiqin Lv / Junxia Wang / Li Xin / XuFeng Dong / Tao Xu / Yuan Nan / Hua Wei / Xinting Chai / Na Li / Yan Ni / Yun Shang / Lizhen Zhang /

Ye Zhao

BMC Women's Health, Vol 21, Iss 1, Pp 1-

2021 Volume 7

Abstract: Abstract Background This study aimed to investigate the clinical and pathological characteristics, and the recurrence and prognostic factors of borderline ovarian tumors (BOTs). Methods The data of 286 patients admitted to hospital and followed up for ... ...

Abstract	Abstract Background This study aimed to investigate the clinical and pathological characteristics, and the recurrence and prognostic factors of borderline ovarian tumors (BOTs). Methods The data of 286 patients admitted to hospital and followed up for more than ten months were analyzed retrospectively to study the clinicopathological characteristics and related factors of recurrence. Results The median age of the patients was 42.06 ± 14.97 years, and the duration of the follow-up ranged from 10–109 months. During the follow-up period, 40 patients had a recurrence. Of these patients, 36 were ≤ 40 years, and patients with premenopausal recurrence accounted for 20.5% (36/176). In patients undergoing conservative treatment or radical operations, the recurrence rates were 21.3% and 1.8%, respectively, and they were 13.4% (36/268) in patients at Federation International of Gynecology and Obstetrics (FIGO) stage I, and 22.2% (4/18) in patients at an advanced stage. Postoperative pathology revealed that 40 patients had micropapillary tumors, among whom ten patients (25%) had a recurrence, and 19 patients had complications with interstitial infiltration. Of these 19 patients, six had a recurrence (31.5%). Another 22 patients had complications with calcified sand bodies; among these, eight patients (36.4%) had a recurrence. All the differences were statistically significant (P < 0.05). There were four cancer-related deaths during the follow-up period. Late FIGO stage, conservative operation, and a high level of carbohydrate antigen 125 (CA125) were independent risk factors for the recurrence of BOTs. Conclusion BOTs usually occur in women under 40 years, have an occult onset, and half of the patients have no obvious clinical manifestations. Serum CA125 level can be used as a tumor marker to detect BOTs and the risk of its recurrence. Operation mode and FIGO stage are important independent factors for the recurrence of BOTs.
Keywords	Ovarian borderline tumor ; Recurrence ; FIGO staging ; Conservative surgery ; CA125 ; Gynecology and obstetrics ; RG1-991 ; Public aspects of medicine ; RA1-1270
Subject code	610 ; 616
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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