LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 35

Search options

  1. Article ; Online: Long Exposure to Salt Stress in Jatropha curcas Leads to Stronger Damage to the Chloroplast Ultrastructure and Its Functionality Than the Stomatal Function

    Huijuan Cao / Yongguang Han / Ziyi Cheng / Qian Lv / Marcelo F. Pompelli / Jaqueline Dias Pereira / Wagner L. Araújo

    Forests, Vol 14, Iss 1868, p

    2023  Volume 1868

    Abstract: As sessile organisms, plants face a wide range of abiotic stresses, with salinity being a significant condition affecting their growth, development, and productivity, particularly in arid and semi-arid regions. This study focused on understanding how ... ...

    Abstract As sessile organisms, plants face a wide range of abiotic stresses, with salinity being a significant condition affecting their growth, development, and productivity, particularly in arid and semi-arid regions. This study focused on understanding how salinity impacts Jatropha curcas , an important oilseed plant for the production of biodiesel. By examining the anatomy and ultrastructure of stomata and chloroplasts, we investigated the effects of prolonged salinity stress on J. curcas . This stress led to changes in the stomatal density, stomatal index, and ostiole aperture, which can cause an imbalance of water conductivity in the xylem. Through transmission electron microscopy, we explored the subcellular organization of J. curcas chloroplasts and their contribution to plant photosynthetic efficiency, providing insights into their role in this process. Notably, increases in salinity resulted in a significant increase in starch granule accumulation, leading to impaired granal and stromal grana lamellae, destroying this ultrastructure. Our findings indicate that the anatomy and ultrastructure of chloroplasts play a crucial role in influencing photosynthetic efficiency. Moreover, impaired hydraulic conductivity due to salinity and a lesser osmotic potential in vessels may cause a reduced source-to-sink relationship, which increases starch accumulation in the chloroplast and influences the ultrastructure of the chloroplast. This study offers a new perspective on the structure and function of chloroplasts in J. curcas , presenting innovative opportunities to develop strategies that enhance the production of biofuel in areas with high soil salinity.
    Keywords purging nut ; scanning electron microscopy ; transmission electron microscopy ; chloroplast ultrastructure ; NaCl long exposition time ; hydraulic conductivity ; Plant ecology ; QK900-989
    Subject code 580
    Language English
    Publishing date 2023-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  2. Article ; Online: Effects of acupuncture treatment on postoperative gastrointestinal dysfunction in colorectal cancer

    Xueyan Liu / Zhijie Wang / Hao Yao / Yanrong Yang / Huijuan Cao / Zhanhao Toh / Ruwen Zheng / Yi Ren

    Trials, Vol 23, Iss 1, Pp 1-

    study protocol for randomized controlled trials

    2022  Volume 12

    Abstract: Abstract Background Postoperative gastrointestinal dysfunction (PGID) is a common complication arising from colorectal cancer surgery. Attributing factors, such as anesthesia, surgical retraction, and early intake of water, can inhibit gastrointestinal ... ...

    Abstract Abstract Background Postoperative gastrointestinal dysfunction (PGID) is a common complication arising from colorectal cancer surgery. Attributing factors, such as anesthesia, surgical retraction, and early intake of water, can inhibit gastrointestinal motility, causing constipation, reduction or absence of bowel sounds, nausea, vomiting, and other symptoms. Delayed recovery in gastrointestinal function can lead to intestinal obstructions or paralysis, anastomotic leaks, and other complications, affecting the patient’s recovery and quality of life negatively. Due to its complex pathophysiology, treatment for PGID in colorectal patients has remained a challenge. Acupuncture is an alternative therapy commonly used for postoperative recovery. This study aims to evaluate the therapeutic efficacy and safety of acupuncture on PGID. Through the complementation of acupuncture and enhanced recovery after surgery (ERAS) protocols, the advantages of acupuncture treatments could be demonstrated to promote its application in future clinical practice. Methods The study design is a prospective randomized controlled trial (RCT). One hundred sixty postoperative colorectal cancer patients will be recruited from Cancer Hospital Chinese Academy of Medical Sciences (CICAMS). Subjects who fulfill inclusion criteria will be randomly assigned into the acupuncture group (AG) (n = 80) or control group (CG) (n = 80). AG will receive acupuncture treatment and perioperative care guided by ERAS protocols, and CG will only receive perioperative care guided by ERAS protocols. The intervention will begin on the first day post-surgery, continuing for 4 days, with a follow-up assessment in a month. Time of first postoperative flatus would be the primary outcome measure. Secondary outcome measures include the time of first postoperative defecation, time of first fluid intake, time of first ambulation, postoperative hospital stay, gastrointestinal reaction score, acupuncture sensation evaluation scale, laboratory tests, postoperative quality of life, readmission rate, and postoperative complications. All results are evaluated from baseline, post-treatment, and upon follow-up. Discussion The results of the study would help elucidate evidence of the therapeutic effects of acupuncture on the recovery of postoperative gastrointestinal function. The objective of the study aims for the eventual inclusion of acupuncture in the ERAS protocol, allowing for wider application in clinical practice. Trial registration ClinicalTrials.gov ChiCTR2000036351. Registered on August 22, 2020
    Keywords Colorectal cancer ; Postoperative ; Acupuncture ; Gastrointestinal function ; Enhanced recovery after surgery ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  3. Article ; Online: Does tai chi improve psychological well-being and quality of life in patients with cardiovascular disease and/or cardiovascular risk factors? A systematic review

    Guoyan Yang / Wenyuan Li / Nerida Klupp / Huijuan Cao / Jianping Liu / Alan Bensoussan / Hosen Kiat / Diana Karamacoska / Dennis Chang

    BMC Complementary Medicine and Therapies, Vol 22, Iss 1, Pp 1-

    2022  Volume 19

    Abstract: Abstract Background Psychological risk factors have been recognised as potential, modifiable risk factors in the development and progression of cardiovascular disease (CVD). Tai Chi, a mind-body exercise, has the potential to improve psychological well- ... ...

    Abstract Abstract Background Psychological risk factors have been recognised as potential, modifiable risk factors in the development and progression of cardiovascular disease (CVD). Tai Chi, a mind-body exercise, has the potential to improve psychological well-being and quality of life. We aim to assess the effects and safety of Tai Chi on psychological well-being and quality of life in people with CVD and/or cardiovascular risk factors. Methods We searched for randomised controlled trials evaluating Tai Chi for psychological well-being and quality of life in people with CVD and cardiovascular risk factors, from major English and Chinese databases until 30 July 2021. Two authors independently conducted study selection and data extraction. Methodological quality was evaluated using the Cochrane Risk of Bias tool. Review Manager software was used for meta-analysis. Results We included 37 studies (38 reports) involving 3525 participants in this review. The methodological quality of the included studies was generally poor. Positive effects of Tai Chi on stress, self-efficacy, and mood were found in several individual studies. Meta-analyses demonstrated favourable effects of Tai Chi plus usual care in reducing anxiety (SMD − 2.13, 95% confidence interval (CI): − 2.55, − 1.70, 3 studies, I 2 = 60%) and depression (SMD -0.86, 95% CI: − 1.35, − 0.37, 6 studies, I 2 = 88%), and improving mental health (MD 7.86, 95% CI: 5.20, 10.52, 11 studies, I 2 = 71%) and bodily pain (MD 6.76, 95% CI: 4.13, 9.39, 11 studies, I 2 = 75%) domains of the 36-Item Short Form Survey (scale from 0 to 100), compared with usual care alone. Tai Chi did not increase adverse events (RR 0.50, 95% CI: 0.21, 1.20, 5 RCTs, I 2 = 0%), compared with control group. However, less than 30% of included studies reported safety information. Conclusions Tai Chi seems to be beneficial in the management of anxiety, depression, and quality of life, and safe to practice in people with CVD and/or cardiovascular risk factors. Monitoring and reporting of safety information ...
    Keywords Tai Chi ; Stress ; Depression ; Anxiety ; Quality of life ; Cardiovascular disease ; Other systems of medicine ; RZ201-999
    Subject code 150
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  4. Article ; Online: The Velvet Protein UvVEA Regulates Conidiation and Chlamydospore Formation in Ustilaginoidea virens

    Mina Yu / Junjie Yu / Huijuan Cao / Xiayan Pan / Tianqiao Song / Zhongqiang Qi / Yan Du / Shiwen Huang / Yongfeng Liu

    Journal of Fungi, Vol 8, Iss 479, p

    2022  Volume 479

    Abstract: Rice false smut, caused by Ustilaginoidea virens , is a serious disease of rice worldwide, severely reducing the quantity and quality of rice production. The conserved fungal velvet proteins are global regulators of diverse cellular processes. We ... ...

    Abstract Rice false smut, caused by Ustilaginoidea virens , is a serious disease of rice worldwide, severely reducing the quantity and quality of rice production. The conserved fungal velvet proteins are global regulators of diverse cellular processes. We identified and functionally characterized two velvet genes, UvVEA and UvVELB , in U. virens . The deletion of these genes affected the conidiation of U. virens but had no effect on the virulence of this pathogen. Interestingly, the Δ UvVEA mutants appeared in the form of smaller false smut balls with a reduced number of chlamydospores compared with the wide-type strains. In addition, the deletion of UvVEA affected the expression of some transmembrane transport genes during chlamydospore formation and rice false smut balls development. Furthermore, the Δ UvVEA mutants were shown to be defective in the utilization of glucose. These findings proved the regulatory mechanism underlying the formation of rice false smut balls and chlamydospores and provided a basis for the further exploration of the mechanism of these processes.
    Keywords rice false smut ; Ustilaginoidea virens ; velvet ; conidia ; chlamydospores ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  5. Article ; Online: Oral herbal medicine for women with intrahepatic cholestasis in pregnancy

    Ruiting Wang / Nuo Cheng / Rongyan Peng / Zeyu Yu / Mengdie Nan / Huijuan Cao

    BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-

    a systematic review of randomized controlled trials

    2020  Volume 24

    Abstract: Abstract Background Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy complication whose range has been calculated to be between 0.01 and 15.6% all around the world. We wanted to systematically evaluate the effect and safety of oral herbal ... ...

    Abstract Abstract Background Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy complication whose range has been calculated to be between 0.01 and 15.6% all around the world. We wanted to systematically evaluate the effect and safety of oral herbal medicine on treatment for ICP. Methods Details of the methods could be found in the registered protocol on PROSPERO (CRD42018096013). Trials assessing the effectiveness of herbal medicine for ICP were searched from seven electronic databases from inception to 28th February 2020. RevMan 5.3 software was used to perform all statistical analysis. Meta-analysis, additional analysis, Trial Sequential Analysis (TSA) and Grading of Recommendations Assessment, Development and Evaluation (GRADE) were conducted if data permitted. Results Totally 43 randomized controlled trials with 3556 patients were included. Meta-analysis showed potential good adjunctive effect of herbal medicine on decreasing the pruritus scores (MD -0.58, 95% CI − 0.79 to − 0.36), the serum TBA scores (MD − 3.99 μmol/L, 95% CI − 4.24 to − 3.74) on the basis with Ursodesoxycholic acid. Compared to the medicine alone, significantly lower incidence of fetal distress (RR 0.41, 95% CI 0.32 to 0.51), asphyxia neonatorum (RR 0.35, 95%CI 0.25 to 0.49), cesarean section (RR 0.73, 95% CI 0.63 to 0.85), postpartum hemorrhage (RR 0.45, 95% CI 0.28 to 0.72) were observed in the combination group. But the comparison between herbal medicine and medicine showed inconsistent results among trials. Insufficient information could be used to evaluate the safety of herbal medicine for ICP. Conclusion This review found the current evidence may support the effectiveness of combination of herbal medicine and conventional medicine for decreasing the maternal pruritus scores, the serum TBA, and the number of fetal distress, or asphyxia neonatorum events related to this condition (which was supported by TSA results). Since there were obvious statistical and clinical heterogeneity among trials, and the methodological quality of the ...
    Keywords Herbal medicine ; Intrahepatic cholestasis ; Meta-analysis ; Pregnancy ; Systematic review ; Other systems of medicine ; RZ201-999
    Subject code 610
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  6. Article ; Online: The impact of recipient age on the effects of umbilical cord mesenchymal stem cells on HBV-related acute-on-chronic liver failure and liver cirrhosis

    Ka Zhang / Haixia Sun / Huijuan Cao / Yifan Jia / Xin Shu / Hong Cao / Yufeng Zhang / Xiaoan Yang

    Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-

    2021  Volume 10

    Abstract: Abstract Background The results of a previous study verified that umbilical cord mesenchymal stem cells (UCMSCs) have good therapeutic effects for the treatment of HBV-related acute-on-chronic liver failure (ACLF) and liver cirrhosis (LC). Nevertheless, ... ...

    Abstract Abstract Background The results of a previous study verified that umbilical cord mesenchymal stem cells (UCMSCs) have good therapeutic effects for the treatment of HBV-related acute-on-chronic liver failure (ACLF) and liver cirrhosis (LC). Nevertheless, it is still unknown whether the effects of UCMSCs are affected by recipient age. Methods Patients treated with UCMSCs who met the criteria of HBV-related ACLF and liver cirrhosis were identified in this retrospective observational study. Patients were divided into subgroups according to the World Health Organization (WHO) age criteria (< 45 vs. ≥ 45 years). Group A included young ACLF patients (< 45 y), and group B included older ACLF patients (≥ 45 y). Young LC patients (< 45 y) were assigned to group C, and group D included older LC patients (≥ 45 y). Patients’ clinical characteristics, demographics, biochemical factors, and model for end-stage liver disease (MELD) scores were compared for 24 weeks. Results Sixty-four ACLF patients and 59 LC patients were enrolled in this study. Compared with patients in groups B and C, patients in group A did not show significant superiority in terms of the levels of ALT, AST, TBIL, AFP, and PTA and MELD scores. However, the median decrease and cumulative decrease in the TBIL and ALT levels of patients in group C were larger than those of patients in group D after four weeks of UCMSC transfusions. For older patients (≥ 45 y), the cumulative decrease and the median decrease in the TBIL of ACLF patients were significantly greater than those of LC patients after UCMSC treatment. However, the median decrease in ALT levels of ACLF patients was significantly greater than that of LC patients during UCMSC treatment, and the cumulative decrease in ALT levels of ACLF patients was significantly greater than that of LC patients at all time points. Conclusion The therapeutic effects of UCMSCs for HBV-related acute-on-chronic liver failure and liver cirrhosis varied partly by patient age. Assessing patient age is necessary prior to ...
    Keywords Umbilical cord mesenchymal stem cell transplantation ; Liver failure ; Liver cirrhosis ; Hepatitis B virus ; Age factors ; Medicine (General) ; R5-920 ; Biochemistry ; QD415-436
    Subject code 610
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  7. Article ; Online: Dynamics of Pyricularia oryzae Population Race Structures from 2003 to 2017 in Jiangsu Province, China

    Zhongqiang Qi / Yan Du / Muxing Liu / Junjie Yu / Rongsheng Zhang / Mingna Yu / Huijuan Cao / Tianqiao Song / Xiayan Pan / Dong Liang / Yongfeng Liu

    Agronomy, Vol 12, Iss 956, p

    2022  Volume 956

    Abstract: Rice blast, caused by Pyricularia oryzae , is one of the most destructive rice diseases worldwide. It is essential to understand the population structure and race distribution of P. oryzae for the prevention and control of rice blast. This study ... ...

    Abstract Rice blast, caused by Pyricularia oryzae , is one of the most destructive rice diseases worldwide. It is essential to understand the population structure and race distribution of P. oryzae for the prevention and control of rice blast. This study collected 1584 isolates across Jiangsu province from 2003 to 2017 to determine race diversity, common and dominant race structures, and resistance profiles using eight parameters from the Chinese differential cultivars (CDC) entries. Race diversity analysis indicated that the race diversity of 1584 isolates in Jiangsu province increased from 2003 to 2006 and fluctuated steadily from 2007 to 2017. Common race structure analysis showed that the common race structure of the isolates fluctuated wildly on an annual basis. Moreover, the race ZG1 was the dominant race, suggesting that the most commonly grown rice varieties in Jiangsu are highly adaptive to race ZG1. In addition, due to a higher level (>85%) of resistance to the population of isolates from 2003 to 2017, Tetep may be conformed as the most promising donor of blast resistance for resistance breeding in Jiangsu province. In summary, it is beneficial to control rice blast by continuous monitoring of the population structure and distribution of P. oryzae with the monogenic germplasm entries and by maintaining a diversity of rice varieties.
    Keywords Pyricularia oryzae ; CDC entries ; race diversity ; race ZG1 ; resistance ; Agriculture ; S
    Subject code 950
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  8. Article: Structural elucidation and anti-osteoporosis activities of polysaccharides obtained from Curculigo orchioides

    Wang, Xueqian / Chunyan Yan / Dawei Zhang / Huijuan Cao / Mengliu Zhang / Qian Zhang / Xinluan Wang

    Carbohydrate polymers. 2019 Jan. 01, v. 203

    2019  

    Abstract: Curculigo orchioides, is a traditional Chinese medicine, is used in strengthening tendons and bones. We evaluated the anti-osteoporosis activity of the crude polysaccharide (CO70) isolated from the rhizomes of C. orchioides in ovariectomized rats. CO70 ... ...

    Abstract Curculigo orchioides, is a traditional Chinese medicine, is used in strengthening tendons and bones. We evaluated the anti-osteoporosis activity of the crude polysaccharide (CO70) isolated from the rhizomes of C. orchioides in ovariectomized rats. CO70 showed excellent anti-osteoporosis activity comparable to that of 17β-estradiol. To explore the constituents responsible for the anti-osteoporosis activity of CO70, a novel homogeneous heteropolysaccharide, COP70-3, was isolated and purified from CO70. COP70-3 has a main backbone chain of (1→5)-linked α-L-Araf, (1,3→5)-linked α-L-Araf, (1→6)-linked β-D-Galp, (1→4)-linked β-D-Manp, (1,2→5)-linked α-L-Araf, (1→3)-linked β-L-Rhap, (1, 3→6)-linked β-D-Manp, (1→3)-linked α-D-GalpA, (1,3→6)-linked β-D-Galp and (1→6)-linked α-D-Glcp residues. Furthermore, 1.87 nM COP70-3 obviously promoted the differentiation of MC3T3-E1 cells, while 0.94 and 1.87 nM COP70-3 significantly improved the osteogenic mineralization rate. These data indicate that COP70-3 has favorable anti-osteoporosis activity in vitro.
    Keywords bone formation ; bones ; Curculigo orchioides ; estradiol ; mineralization ; Oriental traditional medicine ; ovariectomy ; polysaccharides ; rats ; rhizomes ; tendons
    Language English
    Dates of publication 2019-0101
    Size p. 292-301.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2018.09.059
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

  9. Article ; Online: Enhanced therapeutic effects of umbilical cord mesenchymal stem cells after prolonged treatment for HBV-related liver failure and liver cirrhosis

    Yifan Jia / Xin Shu / Xiaoan Yang / Haixia Sun / Huijuan Cao / Hong Cao / Ka Zhang / Qihuan Xu / Gang Li / Yang Yang

    Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-

    2020  Volume 10

    Abstract: Abstract Background Umbilical cord mesenchymal stem cells (UCMSCs) have been demonstrated to have good therapeutic effects in the treatment of HBV-related liver diseases. However, the therapeutic effect of UCMSCs on HBV-related liver failure and liver ... ...

    Abstract Abstract Background Umbilical cord mesenchymal stem cells (UCMSCs) have been demonstrated to have good therapeutic effects in the treatment of HBV-related liver diseases. However, the therapeutic effect of UCMSCs on HBV-related liver failure and liver cirrhosis and the variations in the efficacy of UCMSCs after different treatment courses remain poorly understood. Therefore, this study was designed to answer these two questions. Methods This was an observational study that retrospectively considered a 3-year period during which 513 patients who received stem cell infusion and met the criteria of hepatic failure and liver cirrhosis were identified from the databases of the Third Affiliated Hospital of Sun Yat-sen University. The eligible patients were categorized into the liver failure group and liver cirrhosis group. The two groups were divided into different subgroups according to the duration of stem cell therapy. In the liver failure group, group A received more than 4 weeks and group B received less than 4 weeks of stem cell therapy. In the liver cirrhosis group, patients who received more than 4 weeks of stem cell therapy belonged to group C, and the patients in group D received less than 4 weeks of stem cell therapy. The patients were followed up for 24 weeks. The demographics, clinical characteristics, biochemical factors, and model for end-stage liver disease (MELD) scores were recorded and compared among different groups. Results A total of 64 patients met the criteria for liver failure, and 59 patients met the criteria for liver cirrhosis. After UCMSC treatment, the levels of alanine aminotransferase (ALT), glutamic-oxaloacetic transaminase (AST), and total bilirubin (TBIL) at all postbaseline time points were significantly lower than those at baseline in the liver failure group and liver cirrhosis group; the prothrombin activity (PTA) and MELD scores gradually improved in only the liver failure group. Four weeks after UCMSC treatment, patients who received prolonged treatment with UCMSCs had a larger ...
    Keywords Umbilical cord mesenchymal stem cell transplantation ; Hepatitis B virus ; Liver failure ; Liver cirrhosis ; Therapeutic effects ; Medicine (General) ; R5-920 ; Biochemistry ; QD415-436
    Subject code 610
    Language English
    Publishing date 2020-07-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  10. Article: UvSMEK1, a Suppressor of MEK Null, Regulates Pathogenicity, Conidiation and Conidial Germination in Rice False Smut Fungus Ustilaginoidea virens

    Junjie, Yu / Mina, Yu / Tianqiao, Song / Huijuan, Cao / Mingli, Yong / Xiayan, Pan / Zhongqiang, Qi / Yan, Du / Rongsheng, Zhang / Xiaole, Yin / Dong, Liang / Yongfeng, Liu

    Rice science. 2021 Sept., v. 28, no. 5

    2021  

    Abstract: Rice false smut, which is caused by Ustilaginoidea virens, is an emerging disease of rice spikelets in rice-growing areas worldwide. However, the infection mechanism of U. virens on rice spikelets is still unclear. Here, we characterized a suppressor of ... ...

    Abstract Rice false smut, which is caused by Ustilaginoidea virens, is an emerging disease of rice spikelets in rice-growing areas worldwide. However, the infection mechanism of U. virens on rice spikelets is still unclear. Here, we characterized a suppressor of mitogen-activated protein kinase kinase or ERK kinase (MEK) null (UvSMEK1) in U. virens that is conserved among filamentous fungi. Compared with wild type U. virens strain P-1, UvSMEK1 deletion mutants were defective in pathogenicity and conidial germination. In addition, conidiation of UvSMEK1 deletion mutants was significantly reduced on yeast extract tryptone (YT) plates, but increased in YT broth compared with the wild type. Compared with UvSMEK1 expression level during the vegetative mycelia and conidiation stages, UvSMEK1 dramatically increased during infection of rice florets. Surprisingly, the UvSMEK1 deletion mutants exhibited higher tolerance to H₂O₂ and NaCl. In summary, presented evidence suggested that UvSMEK1 positively regulated pathogenicity, conidial germination and conidiation in YT broth, and negatively regulated conidiation on YT medium and tolerance to oxidative and osmotic stresses. The results enhance our understanding of the regulatory mechanism of pathogenicity of U. virens, and present a potential molecular target for blocking rice infection by U. virens.
    Keywords Ustilaginoidea virens ; conidia ; conidiation ; florets ; germination ; mitogen-activated protein kinase kinase ; mycelium ; pathogenicity ; rice ; rice false smut ; spikelets ; tryptones ; yeast extract
    Language English
    Dates of publication 2021-09
    Size p. 457-465.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2406776-3
    ISSN 1876-4762 ; 1672-6308
    ISSN (online) 1876-4762
    ISSN 1672-6308
    DOI 10.1016/j.rsci.2021.07.006
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

To top