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Article ; Online: DNA damage repair-related gene signature for identifying the immune status and predicting the prognosis of hepatocellular carcinoma

Yongpan Lu / Sen Wang / Tingting Chi / Yuli Zhao / Huimin Guo / Haizheng Wang / Li Feng

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 18

Abstract: Abstract The heterogeneity of hepatocellular carcinoma (HCC) poses a challenge for accurate prognosis prediction. DNA damage repair genes (DDRGs) have an impact on a wide range of malignancies. However, the relevance of these genes in HCC prognosis has ... ...

Abstract	Abstract The heterogeneity of hepatocellular carcinoma (HCC) poses a challenge for accurate prognosis prediction. DNA damage repair genes (DDRGs) have an impact on a wide range of malignancies. However, the relevance of these genes in HCC prognosis has received little attention. In this study, we aimed to develop a prognostic signature to identify novel therapy options for HCC. We acquired mRNA expression profiles and clinical data for HCC patients from The Cancer Genome Atlas (TCGA) database. A polygenic prognostic model for HCC was constructed using selection operator Cox analysis and least absolute shrinkage. The model was validated using International Cancer Genome Consortium (ICGC) data. Overall survival (OS) between the high-risk and low-risk groups was compared using Kaplan‒Meier analysis. Independent predictors of OS were identified through both univariate and multivariate Cox analyses. To determine immune cell infiltration scores and activity in immune-related pathways, a single-sample gene set enrichment analysis was performed. The protein and mRNA expression levels of the prognostic genes between HCC and normal liver tissues were also examined by immunohistochemistry (IHC), immunofluorescence (IF) and quantitative real-time PCR (qRT-PCR). A novel ten-gene signature (CHD1L, HDAC1, KPNA2, MUTYH, PPP2R5B, NEIL3, POLR2L, RAD54B, RUVBL1 and SPP1) was established for HCC prognosis prediction. Patients in the high-risk group had worse OS than those in the low-risk group. Receiver operating characteristic curve analysis confirmed the predictive ability of this prognostic gene signature. Multivariate Cox analysis showed that the risk score was an independent predictor of OS. Functional analysis revealed a strong association with cell cycle and antigen binding pathways, and the risk score was highly correlated with tumor grade, tumor stage, and types of immune infiltrate. High expression levels of the prognostic genes were significantly correlated with increased sensitivity of cancer cells to antitumor drugs. ...
Keywords	Medicine ; R ; Science ; Q
Subject code	610 ; 616
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Music Therapy Combined with Free Position Delivery on Labor Pain and Birth Outcomes

Huimin Guo / Mochun Que / Jie Shen / Qiaole Nie / Youguo Chen / Qin Huang / Aiying Jin

Applied Bionics and Biomechanics, Vol

2022 Volume 2022

Abstract: Objective. At present, the clinical effect of music therapy combined with free position to assist delivery is rarely reported. Based on evidence-based nursing, this study is aimed at exploring the effect of the combination of music therapy and free ... ...

Abstract	Objective. At present, the clinical effect of music therapy combined with free position to assist delivery is rarely reported. Based on evidence-based nursing, this study is aimed at exploring the effect of the combination of music therapy and free position delivery on labor pain and maternal and fetal outcomes. Methods. A total of 440 primiparas with vaginal delivery in the Department of Obstetrics and Gynecology of the First Affiliated Hospital of Soochow University between July 2018 and July 2019 were selected. They were divided into music therapy, free position delivery group (n=201) and traditional delivery group (n=239). Subsequently, the two groups were compared in terms of the Chinese Perception of Labor Pain Questionnaire (PLPQ) score, bleeding amount at 2 hours after birth, perineal injury, labor stage duration, and 1 minute Apgar score. Results. The combination group had better results of the Chinese PLPQ score, postpartum hemorrhage, and perineum condition. However, compared with traditional delivery, a longer duration of the first stage and total stage of labor was found in the combination group. Music therapy combined with free position delivery required less medical intervention during delivery, and there was no significant difference in 1 minute Apgar score between the two groups. Conclusions. Music therapy combined with free position delivery, an intervention based on evidence-based nursing, can effectively reduce maternal labor pain, postpartum hemorrhage, soft birth canal injury, and medical intervention during labor. It is, therefore, a safe intervention to assist delivery.
Keywords	Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5
Subject code	780
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Additional mutations based on Omicron BA.2.75 mediate its further evasion from broadly neutralizing antibodies

Huimin Guo / Jie Jiang / Senlin Shen / Xiangyang Ge / Qing Fan / Bing Zhou / Lin Cheng / Bin Ju / Zheng Zhang

iScience, Vol 26, Iss 4, Pp 106283- (2023)

2023

Abstract: Summary: SARS-CoV-2 Omicron BA.2.75 subvariant has evolved to a series of progeny variants carrying several additional mutations in the receptor-binding domain (RBD). Here, we investigated whether and how these single mutations based on BA.2.75 affect ... ...

Abstract	Summary: SARS-CoV-2 Omicron BA.2.75 subvariant has evolved to a series of progeny variants carrying several additional mutations in the receptor-binding domain (RBD). Here, we investigated whether and how these single mutations based on BA.2.75 affect the neutralization of currently available anti-RBD monoclonal antibodies (mAbs) with well-defined structural information. Approximately 34% of mAbs maintained effective neutralizing activities against BA.2.75, consistent with those against BA.2, BA.4/5, and BA.2.12.1. Single additional R346T, K356T, L452R, or F486S mutations further facilitated BA.2.75-related progeny variants to escape from broadly neutralizing antibodies (bnAbs) at different degree. Only LY-CoV1404 (bebtelovimab) displayed a first-class neutralization potency and breadth against all tested Omicron subvariants. Overall, these data make a clear connection between virus escape and antibody recognizing antigenic epitopes, which facilitate to develop next-generation universal bnAbs against emerging SARS-CoV-2 variants.
Keywords	Immunology ; Virology ; Science ; Q
Subject code	616
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Exploring the psychometric properties of the professional issues in maternal mental health scale (PIMMHS) in a Chinese population

Lu Lin / Huimin Guo / Qian Fang / Colin R. Martin / Aiying Jin / Congyan Xie / Li Tian / Julie Jomeen

Health and Quality of Life Outcomes, Vol 21, Iss 1, Pp 1-

2023 Volume 9

Abstract: Abstract Background The public health and economic implications of perinatal mental health problems are well documented. Maternity clinicians are ideally placed to effectively identify women at risk and facilitate early intervention. However, in China as ...

Abstract	Abstract Background The public health and economic implications of perinatal mental health problems are well documented. Maternity clinicians are ideally placed to effectively identify women at risk and facilitate early intervention. However, in China as globally a number of issues are implicated in a failure to recognise and treat. Aim The present study sought to develop and evaluate the Chinese version ‘professional issues in maternal mental health’ scale (PIMMHS), explore its psychometric properties and potential application. Methods A cross-sectional design and instrument translation and evaluation approach was taken to investigate the psychometric properties of the PIMMHS in a Chinese population. A total of 598 obstetricians, obstetric nurses, and midwives participated in this study from 26 hospitals across China. Findings The Chinese PIMMHS was not a good fit to the original two factor model. The emotion/communication subscale yielded an excellent fit to the data according to all fit indices, offering compelling evidence for a single factor solution. The training (PIMMHS: Training), proved problematic throughout the analysis with divergent validity for the training subscale also being poor with a concomitant impact on the total scale performance. The performance of this subscale may be related to the nature of medical training and PMH. Conclusion The Chinese PIMMHS comprises a unidimensional scale of emotion/ communication, which is simple and may provide insight into the emotional burden of providing PMH care, with the potential to mitigate that burden. Further development and investigation of the training sub-scale could be of value.
Keywords	Perinatal ; Mental health ; Scale ; Psychometric ; Practitioner ; Computer applications to medicine. Medical informatics ; R858-859.7
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Role and Mechanism of lncRNA-pvt1 in the Pathogenesis of Acute Lymphoblastic Leukemia in Children

Yanli Cheng / Guoping Hao / Xiaohuan Wang / Haiyan Lu / Jing Wang / Taoli Suo / Zhaohui Hou / Huimin Guo / Shixun Zhang

Computational and Mathematical Methods in Medicine, Vol

2022 Volume 2022

Abstract: Objective. To investigate the role and mechanism of lncRNA-pvt1 in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). Methods. The expression of lncRNA-pvt1 in bone marrow tissues of ALL patients after initial diagnosis and complete ... ...

Abstract	Objective. To investigate the role and mechanism of lncRNA-pvt1 in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). Methods. The expression of lncRNA-pvt1 in bone marrow tissues of ALL patients after initial diagnosis and complete remission was detected by RT-PCR to explore its possible involvement in the pathogenesis of ALL. The proliferation and apoptosis of Jurkat cells transfected with lncRNA-pvt1 were observed by MTT and flow cytometry. Results. lncRNA-pvt1 expression was upregulated in bone marrow of ALL patients. Knockdown of lncRNA-pvt1 inhibited Jurkat cell proliferation and increased its apoptosis rate. Conclusion. Silencing lncRNA-pvt1 expression can inhibit the development of ALL.
Keywords	Computer applications to medicine. Medical informatics ; R858-859.7
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Multifunctional Waterborne Polyurethane Microreactor-Based Approach to Fluorocarbon Composite Latex Coatings with Double Self-Healing and Excellent Synergistic Performances

Chao Li / Huimin Guo / Ning Zhang / Yao Jin / Kai Han / Jinfeng Yuan / Zhicheng Pan / Mingwang Pan

Nanomaterials, Vol 12, Iss 4216, p

2022 Volume 4216

Abstract: In this article, chlorotrifluoroethylene (CTFE)-based fluorocarbon composite latexes and their coatings are successfully fabricated by an environmentally friendly preparation method based on a new multifunctional waterborne polyurethane (MFWPU) ... ...

Abstract	In this article, chlorotrifluoroethylene (CTFE)-based fluorocarbon composite latexes and their coatings are successfully fabricated by an environmentally friendly preparation method based on a new multifunctional waterborne polyurethane (MFWPU) dispersion. It is worth noting that the MFWPU acts as the sole system stabilizer as well as microreactor and simultaneously endows the composite coating with excellent double self-healing performance and adhesion. Moreover, the introduction of a dynamic disulfide bond in the polyurethane dispersion entrusts the coating with excellent scratch self-healing performance. Simultaneously, carbon–carbon double bonds in the polyurethane dispersion increase the compatibility between the core polymer and shell polymer. The fluorine-containing chain segments can be distributed in the coating evenly during the self-assembly film-forming process of composite particles so that the original element composition of the worn coating surface can restore the original element composition after heating, and the coating presents a regeneration ability, which further and verifies the usefulness of the double self-healing model of the coating. Afterward, efficient recovery and durability, which are two contradictory properties of scratch self-healing polymers, are optimized to obtain a composite coating with excellent comprehensive performance. The research results regarding the composite system may provide a valuable reference for the structural design and application of waterborne fluorocarbon functional coatings in the future.
Keywords	double self-healing model ; multifunctional waterborne polyurethane ; soap-free emulsion polymerization ; Chemistry ; QD1-999
Subject code	620
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The Role of the Respiratory Therapy Team in the Treatment of Patients With Acquired Immunodeficiency Syndrome Complicated With Pneumocystis Pneumonia Undergoing Mechanical Ventilation

Yanmei Gu / Shuangmei Xi / Boxun Jin / Huimin Guo / Jiankun Yang / Xin Liu / Xiulian Wu / Lili Zhang / Guangming Li

Frontiers in Public Health, Vol

2022 Volume 10

Abstract: ObjectiveTo explore the role of the respiratory therapy team in the treatment of patients with acquired immunodeficiency syndrome (AIDS) complicated with pneumocystis pneumonia (PCP) undergoing mechanical ventilation.MethodsA retrospective cross- ... ...

Abstract	ObjectiveTo explore the role of the respiratory therapy team in the treatment of patients with acquired immunodeficiency syndrome (AIDS) complicated with pneumocystis pneumonia (PCP) undergoing mechanical ventilation.MethodsA retrospective cross-sectional study was conducted, including 60 patients with AIDS complicated with PCP undergoing mechanical ventilation in our hospital from June 2019 to July 2020. In the process of patient respiratory monitoring, hospital transport, ventilator withdrawal, airway management, various aerosol treatments and controlled oxygen therapy, patients were divided into the control group and the case group according to whether the respiratory therapy team was involved or not (30 in the control group, 25 males and five females; 30 in the case group, 24 males and six females). The baseline data, mechanical ventilation time, hospitalization time and hospitalization expenses of the two groups were compared.ResultsThere was no statistically significant difference in baseline data between the case and control groups (P > 0.05). Compared with the control group, the case group had significantly shorter mechanical ventilation times and average hospitalization lengths and the average expenses decreased, and the difference was statistically significant (P < 0.05).ConclusionThe participation of the respiratory therapy team in the mechanical ventilation treatment of patients with AIDS and PCP helps to shorten the mechanical ventilation time and the average length of hospitalization and reduce the hospitalization expenses of patients. It is expected to increase the cure rate of such patients and improve their prognosis.
Keywords	respiratory care team ; aids ; PCP ; nursing ; mechanically ventilated patients ; Public aspects of medicine ; RA1-1270
Subject code	610
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Efficient Photooxidation of Sulfides with Amidated Alloxazines as Heavy-atom-free Photosensitizers

Huimin Guo / Hongyu Xia / Xiaolin Ma / Kepeng Chen / Can Dang / Jianzhang Zhao / Bernhard Dick

ACS Omega, Vol 5, Iss 18, Pp 10586-

2020 Volume 10595

Keywords	Chemistry ; QD1-999
Language	English
Publishing date	2020-04-01T00:00:00Z
Publisher	American Chemical Society
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Overexpression of Soybean-Derived Lunasin in Wheat and Assessment of Its Anti-Proliferative Activity in Colorectal Cancer HT-29 Cells

Xin Fan / Peiyou Qin / Yuqiong Hao / Huimin Guo / Christophe Blecker / Nadia Everaert / Guixing Ren

International Journal of Molecular Sciences, Vol 21, Iss 9594, p

2020 Volume 9594

Abstract: Lunasin is a soybean-derived peptide that exhibits anticancer bioactivity in different cancer cells and has been identified in different plants. However, recent studies revealed through molecular and chemical analyses that lunasin was absent in wheat and ...

Abstract	Lunasin is a soybean-derived peptide that exhibits anticancer bioactivity in different cancer cells and has been identified in different plants. However, recent studies revealed through molecular and chemical analyses that lunasin was absent in wheat and other cereals. In this study, the soybean-derived lunasin was cloned into pCAMBIA3300 and we transferred the expression vector into wheat via an Agrobacterium -mediated transformation. The identification of transgenic wheat was detected by polymerase chain reaction, Western blot analysis, and ultra-performance liquid chromatography with tandem mass spectrometry. An enzyme-linked immunosorbent assay showed that lunasin content in transgenic wheat L32-3, L32-6, and L33-1 was 308.63, 436.78, and 349.07 µg/g, respectively, while lunasin was not detected in wild-type wheat. Lunasin enrichment from transgenic wheat displayed an increased anti-proliferative activity compared with peptide enrichment from wild-type wheat in HT-29 cells. Moreover, the results of a real-time quantitative polymerase chain reaction showed a significant elevation in p21, Bax, and caspase-3 expression, while Bcl-2 was significantly downregulated. In conclusion, soybean-derived lunasin was successfully expressed in wheat via Agrobacterium -mediated transformation and may exert anti-proliferative activity by regulating the apoptosis pathway in HT-29 cells, which provides an effective approach to compensate for the absence of lunasin in wheat.
Keywords	transformation ; transgenic ; UPLC-MS/MS ; cytotoxic ; apoptosis ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	571
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Pathogenicity Detection and Genome Analysis of Two Different Geographic Strains of BmNPV

Huimin Guo / Benzheng Zhang / Xin Zheng / Juan Sun / Huiduo Guo / Gang Li / Guodong Zhao / Anying Xu / Heying Qian

Insects, Vol 12, Iss 890, p

2021 Volume 890

Abstract: The pathogenicity of different concentrations of Bombyx mori nuclear polyhedrosis virus- Zhenjiang strain (BmNPV ZJ) and Yunnan strain (BmNPV YN) was assessed in Baiyu larvae. The structures of the two viral strains were observed by negative-staining ... ...

Abstract	The pathogenicity of different concentrations of Bombyx mori nuclear polyhedrosis virus- Zhenjiang strain (BmNPV ZJ) and Yunnan strain (BmNPV YN) was assessed in Baiyu larvae. The structures of the two viral strains were observed by negative-staining electron microscopy, and their proliferation was examined by quantitative polymerase chain reaction (qPCR). The genomic sequences of these two viruses were obtained to investigate the differences in their pathogenicity. The lethal concentration 50 (LC 50 ) of BmNPV ZJ against Baiyu larvae was higher than that of BmNPV YN, indicating a relatively more robust pathogenicity in BmNPV YN. Electron microscopic images showed that the edges of BmNPV YN were clearer than those of BmNPV ZJ. The qPCR analysis demonstrated significantly higher relative expressions of immediately early 1 gene ( ie-1 ), p143 , vp39 , and polyhedrin genes ( polh ) in BmNPV ZJ than in BmNPV YN at 12–96 h. The complete genomes of BmNPV ZJ and BmNPV YN were, respectively, 135,895 bp and 143,180 bp long, with 141 and 145 coding sequences and 40.93% and 39.71% GC content. Considering the BmNPV ZJ genome as a reference, 893 SNP loci and 132 InDel mutations were observed in the BmNPV YN genome, resulting in 106 differential gene sequences. Among these differential genes, 76 (including 22 hub genes and 35 non-hub genes) possessed amino acid mutations. Thirty genes may have been related to viral genome replication and transcription and five genes may have been associated with the viral oral infection. These results can help in understanding the mechanisms of pathogenicity of different strains of BmNPV in silkworms.
Keywords	Bombyx mori nuclear polyhedrosis virus ; sequencing analysis ; qPCR ; pathogenicity difference ; Science ; Q
Subject code	570
Language	English
Publishing date	2021-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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