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  1. Book: GLYCOPROTEIN ANTIGENS IN MULTIPLE SCLEROSIS

    HUKKANEN, VEIJO

    1982  

    Size GETR. ZAEHLUNG
    Document type Book
    Note TURKU, UNIV., DISS., 1982
    HBZ-ID HT002691229
    ISBN 951-99426-9-6 ; 978-951-99426-9-8
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    83 E 1553 order for loan Magazin

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  2. Article: Herpes Simplex Virus Seroprevalence among Pregnant Finnish Women and Their Spouses—A Six-Year Follow-Up Cohort Study

    Laakso, Johanna / Vuorinen, Tytti / Rautava, Jaana / Kero, Katja / Syrjänen, Stina / Hukkanen, Veijo

    Microorganisms. 2022 July 26, v. 10, no. 8

    2022  

    Abstract: The aim was to evaluate the herpes simplex virus (HSV) seroprevalence and seroconversion among 285 pregnant women and their 120 male spouses in Finland during a six-year follow-up (FU) between 1998–2008. We also studied the effect of sexual habits, ... ...

    Abstract The aim was to evaluate the herpes simplex virus (HSV) seroprevalence and seroconversion among 285 pregnant women and their 120 male spouses in Finland during a six-year follow-up (FU) between 1998–2008. We also studied the effect of sexual habits, pregnancy, and other demographic factors on the acquisition of HSV infection. Combined HSV-1 and HSV-2-IgG antibodies were assessed in the first baseline serum samples with an indirect enzyme immunoassay method. The individuals with seronegative or borderline HSV serology at baseline were additionally tested using their latest FU serum sample available. The overall HSV seroprevalence during the FU was 58.9% (168/285) among the women and 53.3% (64/120) among their spouses. The seroconversion rate was 11.4% (15/132) and 12.5% (8/64) among women and their spouses, respectively. Both spouses were HSV seropositive in 39.2% (47/120). To determine the HSV-2 seroprevalence, we also tested all HSV-seropositive participants using HSV-2-specific antigen. HSV-2 seropositivity was detected in 10.9% (44/405) of the participants. The age (p = 0.006) and history of genital warts (p = 0.006) of the women were associated with combined HSV-1 and/or HSV-2 seropositivity, while a younger age was related to HSV seroconversion (p = 0.023). Among the male spouses, HSV seropositivity was associated with the practice of oral sex (p = 0.033). To conclude, women of childbearing age acquire primary HSV infections and the presence of HSV in oral epithelium is common among HSV-seropositive individuals.
    Keywords antigens ; blood serum ; cohort studies ; enzyme immunoassays ; epithelium ; herpes simplex ; males ; pregnancy ; seroconversion ; serology ; seroprevalence ; viruses ; Finland
    Language English
    Dates of publication 2022-0726
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10081506
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Herpes Simplex Virus Seroprevalence among Pregnant Finnish Women and Their Spouses-A Six-Year Follow-Up Cohort Study.

    Laakso, Johanna / Vuorinen, Tytti / Rautava, Jaana / Kero, Katja / Syrjänen, Stina / Hukkanen, Veijo

    Microorganisms

    2022  Volume 10, Issue 8

    Abstract: The aim was to evaluate the herpes simplex virus (HSV) seroprevalence and seroconversion among 285 pregnant women and their 120 male spouses in Finland during a six-year follow-up (FU) between 1998-2008. We also studied the effect of sexual habits, ... ...

    Abstract The aim was to evaluate the herpes simplex virus (HSV) seroprevalence and seroconversion among 285 pregnant women and their 120 male spouses in Finland during a six-year follow-up (FU) between 1998-2008. We also studied the effect of sexual habits, pregnancy, and other demographic factors on the acquisition of HSV infection. Combined HSV-1 and HSV-2-IgG antibodies were assessed in the first baseline serum samples with an indirect enzyme immunoassay method. The individuals with seronegative or borderline HSV serology at baseline were additionally tested using their latest FU serum sample available. The overall HSV seroprevalence during the FU was 58.9% (168/285) among the women and 53.3% (64/120) among their spouses. The seroconversion rate was 11.4% (15/132) and 12.5% (8/64) among women and their spouses, respectively. Both spouses were HSV seropositive in 39.2% (47/120). To determine the HSV-2 seroprevalence, we also tested all HSV-seropositive participants using HSV-2-specific antigen. HSV-2 seropositivity was detected in 10.9% (44/405) of the participants. The age (
    Language English
    Publishing date 2022-07-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10081506
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  4. Article: Native RNA Purification Method for Small RNA Molecules Based on Asymmetrical Flow Field-Flow Fractionation.

    Levanova, Alesia A / Lampi, Mirka / Kalke, Kiira / Hukkanen, Veijo / Poranen, Minna M / Eskelin, Katri

    Pharmaceuticals (Basel, Switzerland)

    2022  Volume 15, Issue 2

    Abstract: RNA molecules provide promising new possibilities for the prevention and treatment of viral infections and diseases. The rapid development of RNA biology and medicine requires advanced methods for the purification of RNA molecules, which allow fast and ... ...

    Abstract RNA molecules provide promising new possibilities for the prevention and treatment of viral infections and diseases. The rapid development of RNA biology and medicine requires advanced methods for the purification of RNA molecules, which allow fast and efficient RNA processing, preferably under non-denaturing conditions. Asymmetrical flow field-flow fractionation (AF4) enables gentle separation and purification of macromolecules based on their diffusion coefficients. The aim of the study was to develop an AF4 method for efficient purification of enzymatically produced antiviral small interfering (si)RNA molecules and to evaluate the overall potential of AF4 in the separation of short single-stranded (ss) and double-stranded (ds) RNA molecules. We show that AF4 separates monomeric ssRNA from dsRNA molecules of the same size and monomeric ssRNA from multimeric forms of the same ssRNA. The developed AF4 method enabled the separation of enzymatically produced 27-nt siRNAs from partially digested substrate dsRNA, which is potentially toxic for mammalian cells. The recovery of AF4-purified enzymatically produced siRNA molecules was about 70%, which is about 20% higher than obtained using anion-exchange chromatography. The AF4-purified siRNAs were not toxic for mammalian cells and fully retained their biological activity as confirmed by efficient inhibition of herpes simplex virus 1 replication in cell culture. Our work is the first to develop AF4 methods for the separation of short RNA molecules.
    Language English
    Publishing date 2022-02-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph15020261
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Herpesvirus vectors in gene therapy.

    Hukkanen, Veijo

    The open virology journal

    2010  Volume 4, Page(s) 94–95

    Language English
    Publishing date 2010-06-18
    Publishing country United Arab Emirates
    Document type Journal Article
    ZDB-ID 2396020-6
    ISSN 1874-3579 ; 1874-3579
    ISSN (online) 1874-3579
    ISSN 1874-3579
    DOI 10.2174/1874357901004030094
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Attenuated Replication-Competent Herpes Simplex Virus Expressing an ECM-Modifying Transgene Hyaluronan Synthase 2 of Naked Mole Rat in Oncolytic Gene Therapy.

    Palomäki, Jussi / Kalke, Kiira / Orpana, Julius / Lund, Liisa / Frejborg, Fanny / Paavilainen, Henrik / Järveläinen, Hannu / Hukkanen, Veijo

    Microorganisms

    2023  Volume 11, Issue 11

    Abstract: Herpes simplex virus (HSV) has proven successful in treating human cancer. Since the approval of talimogene laherparepvec (T-VEC) in 2015, HSV has been thoroughly researched to discover novel mechanisms to combat cancer and treat other diseases. Another ... ...

    Abstract Herpes simplex virus (HSV) has proven successful in treating human cancer. Since the approval of talimogene laherparepvec (T-VEC) in 2015, HSV has been thoroughly researched to discover novel mechanisms to combat cancer and treat other diseases. Another HSV-based drug, beremagene geperpavec (B-VEC), received approval in 2023 to treat the rare genetic disease dystrophic epidermolysis bullosa, and was also the first clinically approved HSV vector carrying an extracellular matrix (ECM)-modifying transgene. The ECM is a network of macromolecules surrounding cells, which provides support and regulates cell growth and differentiation, the disruption of which is common in cancer. The naked mole rat (NMR) has a thick ECM and a unique mutation in the
    Language English
    Publishing date 2023-10-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11112657
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Swarms of chemically modified antiviral siRNA targeting herpes simplex virus infection in human corneal epithelial cells.

    Kalke, Kiira / Lund, Liisa M / Nyman, Marie C / Levanova, Alesia A / Urtti, Arto / Poranen, Minna M / Hukkanen, Veijo / Paavilainen, Henrik

    PLoS pathogens

    2022  Volume 18, Issue 7, Page(s) e1010688

    Abstract: Herpes simplex virus type 1 (HSV-1) is a common virus of mankind and HSV-1 infections are a significant cause of blindness. The current antiviral treatment of herpes infection relies on acyclovir and related compounds. However, acyclovir resistance ... ...

    Abstract Herpes simplex virus type 1 (HSV-1) is a common virus of mankind and HSV-1 infections are a significant cause of blindness. The current antiviral treatment of herpes infection relies on acyclovir and related compounds. However, acyclovir resistance emerges especially in the long term prophylactic treatment that is required for prevention of recurrent herpes keratitis. Earlier we have established antiviral siRNA swarms, targeting sequences of essential genes of HSV, as effective means of silencing the replication of HSV in vitro or in vivo. In this study, we show the antiviral efficacy of 2´-fluoro modified antiviral siRNA swarms against HSV-1 in human corneal epithelial cells (HCE). We studied HCE for innate immunity responses to HSV-1, to immunostimulatory cytotoxic double stranded RNA, and to the antiviral siRNA swarms, with or without a viral challenge. The panel of studied innate responses included interferon beta, lambda 1, interferon stimulated gene 54, human myxovirus resistance protein A, human myxovirus resistance protein B, toll-like receptor 3 and interferon kappa. Our results demonstrated that HCE cells are a suitable model to study antiviral RNAi efficacy and safety in vitro. In HCE cells, the antiviral siRNA swarms targeting the HSV UL29 gene and harboring 2´-fluoro modifications, were well tolerated, induced only modest innate immunity responses, and were highly antiviral with more than 99% inhibition of viral release. The antiviral effect of the 2'-fluoro modified swarm was more apparent than that of the unmodified antiviral siRNA swarm. Our results encourage further research in vitro and in vivo on antiviral siRNA swarm therapy of corneal HSV infection, especially with modified siRNA swarms.
    MeSH term(s) Acyclovir/metabolism ; Acyclovir/pharmacology ; Antiviral Agents/metabolism ; Antiviral Agents/pharmacology ; Epithelial Cells/metabolism ; Herpes Simplex/genetics ; Herpes Simplex/therapy ; Herpesvirus 1, Human/physiology ; Humans ; RNA, Double-Stranded/metabolism ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; Virus Replication/genetics
    Chemical Substances Antiviral Agents ; RNA, Double-Stranded ; RNA, Small Interfering ; Acyclovir (X4HES1O11F)
    Language English
    Publishing date 2022-07-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1010688
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The In Vitro Replication, Spread, and Oncolytic Potential of Finnish Circulating Strains of Herpes Simplex Virus Type 1.

    Kalke, Kiira / Orpana, Julius / Lasanen, Tuomas / Esparta, Olaya / Lund, Liisa M / Frejborg, Fanny / Vuorinen, Tytti / Paavilainen, Henrik / Hukkanen, Veijo

    Viruses

    2022  Volume 14, Issue 6

    Abstract: Herpes simplex virus type 1 (HSV-1) is the only FDA- and EMA- approved oncolytic virus, and accordingly, many potential oncolytic HSVs (oHSV) are in clinical development. The utilized oHSV parental strains are, however, mostly based on laboratory ... ...

    Abstract Herpes simplex virus type 1 (HSV-1) is the only FDA- and EMA- approved oncolytic virus, and accordingly, many potential oncolytic HSVs (oHSV) are in clinical development. The utilized oHSV parental strains are, however, mostly based on laboratory reference strains, which may possess a compromised cytolytic capacity in contrast to circulating strains of HSV-1. Here, we assess the phenotype of thirty-six circulating HSV-1 strains from Finland to uncover their potential as oHSV backbones. First, we determined their capacity for cell-to-cell versus extracellular spread, to find strains with replication profiles favorable for each application. Second, to unfold the differences, we studied the genetic diversity of two relevant viral glycoproteins (gB/UL27, gI/US7). Third, we examined the oncolytic potential of the strains in cells representing glioma, lymphoma, and colorectal adenocarcinoma. Our results suggest that the phenotype of a circulating isolate, including the oncolytic potential, is highly related to the host cell type. Nevertheless, we identified isolates with increased oncolytic potential in comparison with the reference viruses across many or all of the studied cancer cell types. Our research emphasizes the need for careful selection of the backbone virus in early vector design, and it highlights the potential of clinical isolates as backbones in oHSV development.
    MeSH term(s) Finland ; Herpes Simplex/genetics ; Herpesvirus 1, Human/genetics ; Herpesvirus 1, Human/metabolism ; Humans ; Oncolytic Virotherapy/methods ; Oncolytic Viruses/genetics
    Language English
    Publishing date 2022-06-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061290
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: The In Vitro Replication, Spread, and Oncolytic Potential of Finnish Circulating Strains of Herpes Simplex Virus Type 1

    Kalke, Kiira / Orpana, Julius / Lasanen, Tuomas / Esparta, Olaya / Lund, Liisa M. / Frejborg, Fanny / Vuorinen, Tytti / Paavilainen, Henrik / Hukkanen, Veijo

    Viruses. 2022 June 13, v. 14, no. 6

    2022  

    Abstract: Herpes simplex virus type 1 (HSV-1) is the only FDA- and EMA- approved oncolytic virus, and accordingly, many potential oncolytic HSVs (oHSV) are in clinical development. The utilized oHSV parental strains are, however, mostly based on laboratory ... ...

    Abstract Herpes simplex virus type 1 (HSV-1) is the only FDA- and EMA- approved oncolytic virus, and accordingly, many potential oncolytic HSVs (oHSV) are in clinical development. The utilized oHSV parental strains are, however, mostly based on laboratory reference strains, which may possess a compromised cytolytic capacity in contrast to circulating strains of HSV-1. Here, we assess the phenotype of thirty-six circulating HSV-1 strains from Finland to uncover their potential as oHSV backbones. First, we determined their capacity for cell-to-cell versus extracellular spread, to find strains with replication profiles favorable for each application. Second, to unfold the differences, we studied the genetic diversity of two relevant viral glycoproteins (gB/UL27, gI/US7). Third, we examined the oncolytic potential of the strains in cells representing glioma, lymphoma, and colorectal adenocarcinoma. Our results suggest that the phenotype of a circulating isolate, including the oncolytic potential, is highly related to the host cell type. Nevertheless, we identified isolates with increased oncolytic potential in comparison with the reference viruses across many or all of the studied cancer cell types. Our research emphasizes the need for careful selection of the backbone virus in early vector design, and it highlights the potential of clinical isolates as backbones in oHSV development.
    Keywords Human alphaherpesvirus 1 ; adenocarcinoma ; genetic variation ; glioma ; glycoproteins ; lymphoma ; neoplasm cells ; phenotype ; viruses ; Finland
    Language English
    Dates of publication 2022-0613
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061290
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: The virtues and vices of harnessing HSV vectors for CNS autoimmunity modulation.

    Hukkanen, Veijo / Nygårdas, Michaela

    Immunotherapy

    2013  Volume 5, Issue 9, Page(s) 911–913

    MeSH term(s) Animals ; Autoimmunity ; Central Nervous System/immunology ; Central Nervous System/pathology ; Genetic Therapy/methods ; Genetic Vectors/genetics ; Genetic Vectors/therapeutic use ; Herpesvirus 1, Human ; Humans ; Multiple Sclerosis/genetics ; Multiple Sclerosis/immunology ; Multiple Sclerosis/pathology ; Multiple Sclerosis/therapy ; Portraits as Topic ; Transgenes/immunology
    Language English
    Publishing date 2013-09
    Publishing country England
    Document type Editorial ; Research Support, Non-U.S. Gov't ; Review
    ISSN 1750-7448
    ISSN (online) 1750-7448
    DOI 10.2217/imt.13.100
    Database MEDical Literature Analysis and Retrieval System OnLINE

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