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  1. Article: Immunisation with Transgenic

    Topuz Ata, Derya / Hussain, Muattaz / Jones, Michael / Best, Jonathan / Wiese, Martin / Carter, Katharine Christine

    Microorganisms

    2023  Volume 11, Issue 5

    Abstract: Leishmaniasis is a protozoan disease responsible for significant morbidity and mortality. There is no recommended vaccine to protect against infection. In this study, ... ...

    Abstract Leishmaniasis is a protozoan disease responsible for significant morbidity and mortality. There is no recommended vaccine to protect against infection. In this study, transgenic
    Language English
    Publishing date 2023-05-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11051322
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Nanoparticle Isolation from Biological Media for Protein Corona Analysis: The Impact of Incubation and Recovery Protocols on Nanoparticle Properties.

    Daramy, Karim / Punnabhum, Panida / Hussain, Muattaz / Minelli, Caterina / Pei, Yiwen / Rattray, Nicholas J W / Perrie, Yvonne / Rattray, Zahra

    Journal of pharmaceutical sciences

    2023  

    Abstract: Nanoparticles are increasingly implemented in biomedical applications, including the diagnosis and treatment of disease. When exposed to complex biological media, nanoparticles spontaneously interact with their surrounding environment, leading to the ... ...

    Abstract Nanoparticles are increasingly implemented in biomedical applications, including the diagnosis and treatment of disease. When exposed to complex biological media, nanoparticles spontaneously interact with their surrounding environment, leading to the surface-adsorption of small and bio- macromolecules- termed the "corona". Corona composition is governed by nanoparticle properties and incubation parameters. While the focus of most studies is on the protein signature of the nanoparticle corona, the impact of experimental protocols on nanoparticle size in the presence of complex biological media, and the impact of nanoparticle recovery from biological media has not yet been reported. Here using a non-degradable robust model, we show how centrifugation-resuspension protocols used for the isolation of nanoparticles from incubation media, incubation duration and shear flow conditions alter nanoparticle parameters including particle size, zeta potential and total protein content. Our results show significant changes in nanoparticle size following exposure to media containing protein under different flow conditions, which also altered the composition of surface-adsorbed proteins profiled by SDS-PAGE. Our in situ analysis of nanoparticle size in media containing protein using particle tracking analysis highlights that centrifugation-resuspension is disruptive to agglomerates that are spontaneously formed in protein containing media, highlighting the need for in situ analytical methods that do not alter the intermediates formed following nanoparticle exposure to biological media. Nanomedicines are mostly intended for parenteral administration, and our findings show that parameters such as shear flow can significantly alter nanoparticle physicochemical parameters. Overall, we show that the centrifugation-resuspension isolation of nanoparticles from media significantly alters particle parameters in addition to the overall protein composition of surface-adsorbed proteins. We recommend that nanoparticle characterization pipelines studying bio-nano interactions during early nanomedicine development consider biologically-relevant shear flow conditions and media composition that can significantly alter particle physical parameters and subsequent conclusions from these studies.
    Language English
    Publishing date 2023-12-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3151-3
    ISSN 1520-6017 ; 0022-3549
    ISSN (online) 1520-6017
    ISSN 0022-3549
    DOI 10.1016/j.xphs.2023.12.021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.50: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Examination of the effect of niosome preparation methods in encapsulating model antigens on the vesicle characteristics and their ability to induce immune responses.

    Obeid, Mohammad A / Teeravatcharoenchai, Teeranun / Connell, David / Niwasabutra, Kanidta / Hussain, Muattaz / Carter, Katharine / Ferro, Valerie A

    Journal of liposome research

    2020  Volume 31, Issue 2, Page(s) 195–202

    Abstract: Niosome nanoparticles can be prepared using different methods, each of which can affect the size and homogeneity of the prepared particles. The aim of this study was to establish if the method of preparation impacted on the prepared vesicles when loaded ... ...

    Abstract Niosome nanoparticles can be prepared using different methods, each of which can affect the size and homogeneity of the prepared particles. The aim of this study was to establish if the method of preparation impacted on the prepared vesicles when loaded with a model protein and the type of immune responses induced to the vaccine antigen. Niosomes were prepared using both the traditional thin film hydration (TFH) technique and the microfluidic mixing (MM) technique. Influenza antigen was then entrapped in the niosomes and formulations tested for their ability to induce in
    MeSH term(s) Animals ; Drug Delivery Systems ; Immunity ; Liposomes ; Mice ; Mice, Inbred BALB C ; Particle Size ; Surface-Active Agents
    Chemical Substances Liposomes ; Surface-Active Agents
    Language English
    Publishing date 2020-05-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 645173-1
    ISSN 1532-2394 ; 0898-2104
    ISSN (online) 1532-2394
    ISSN 0898-2104
    DOI 10.1080/08982104.2020.1768110
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2539: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Chemical and Antimicrobial Profiling of Propolis from Different Regions within Libya.

    Siheri, Weam / Zhang, Tong / Ebiloma, Godwin Unekwuojo / Biddau, Marco / Woods, Nicola / Hussain, Muattaz Yassein / Clements, Carol J / Fearnley, James / Ebel, RuAngelie Edrada / Paget, Timothy / Muller, Sylke / Carter, Katharine C / Ferro, Valerie A / De Koning, Harry P / Watson, David G

    PloS one

    2016  Volume 11, Issue 5, Page(s) e0155355

    Abstract: Extracts from twelve samples of propolis collected from different regions of Libya were tested for their activity against Trypanosoma brucei, Leishmania donovani, Plasmodium falciparum, Crithidia fasciculata and Mycobacterium marinum and the cytotoxicity ...

    Abstract Extracts from twelve samples of propolis collected from different regions of Libya were tested for their activity against Trypanosoma brucei, Leishmania donovani, Plasmodium falciparum, Crithidia fasciculata and Mycobacterium marinum and the cytotoxicity of the extracts was tested against mammalian cells. All the extracts were active to some degree against all of the protozoa and the mycobacterium, exhibiting a range of EC50 values between 1.65 and 53.6 μg/ml. The toxicity against mammalian cell lines was only moderate; the most active extract against the protozoan species, P2, displayed an IC50 value of 53.2 μg/ml. The extracts were profiled by using liquid chromatography coupled to high resolution mass spectrometry. The data sets were extracted using m/z Mine and the accurate masses of the features extracted were searched against the Dictionary of Natural Products (DNP). A principal component analysis (PCA) model was constructed which, in combination with hierarchical cluster analysis (HCA), divided the samples into five groups. The outlying groups had different sets of dominant compounds in the extracts, which could be characterised by their elemental composition. Orthogonal partial least squares (OPLS) analysis was used to link the activity of each extract against the different micro-organisms to particular components in the extracts.
    MeSH term(s) Animals ; Anti-Infective Agents/chemistry ; Anti-Infective Agents/pharmacology ; Antiprotozoal Agents/chemistry ; Antiprotozoal Agents/pharmacology ; Biological Products/chemistry ; Chromatography, Liquid ; Cluster Analysis ; Crithidia fasciculata/drug effects ; Female ; Geography ; Humans ; Inhibitory Concentration 50 ; Least-Squares Analysis ; Leishmania donovani/drug effects ; Libya ; Mass Spectrometry ; Mice ; Mice, Inbred BALB C ; Microbial Sensitivity Tests ; Mycobacterium marinum/drug effects ; Plant Extracts/chemistry ; Plasmodium falciparum/drug effects ; Principal Component Analysis ; Propolis/chemistry ; Propolis/pharmacology ; Software ; Trypanosoma brucei brucei/drug effects ; U937 Cells
    Chemical Substances Anti-Infective Agents ; Antiprotozoal Agents ; Biological Products ; Plant Extracts ; Propolis (9009-62-5)
    Language English
    Publishing date 2016-05-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0155355
    Database MEDical Literature Analysis and Retrieval System OnLINE

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