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  1. Article ; Online: Prognostic Value of Circulating Cell-Free DNA Concentration and Neutrophil-to-Lymphocyte Ratio in Patients with Pancreatic Ductal Adenocarcinoma: A Prospective Cohort Study.

    Varzaru, Bianca / Iacob, Razvan Andrei / Bunduc, Stefania / Manea, Ioana / Sorop, Andrei / Spiridon, Andreea / Chelaru, Raluca / Croitoru, Adina / Topala, Mihaela / Becheanu, Gabriel / Dumbrava, Mona / Dima, Simona / Popescu, Irinel / Gheorghe, Cristian

    International journal of molecular sciences

    2024  Volume 25, Issue 5

    Abstract: Circulating cell-free DNA (ccfDNA) quantity correlates with the clinical characteristics and prognosis of various cancer types. We investigated whether ccfDNA levels and the neutrophil-to-lymphocyte ratio (NLR) have prognostic value in patients with ... ...

    Abstract Circulating cell-free DNA (ccfDNA) quantity correlates with the clinical characteristics and prognosis of various cancer types. We investigated whether ccfDNA levels and the neutrophil-to-lymphocyte ratio (NLR) have prognostic value in patients with pancreatic ductal adenocarcinoma (PDAC). Peripheral blood was collected from 82 patients with PDAC prior to any diagnostic procedure or the administration of chemotherapy. Plasma DNA was isolated, and ccfDNA concentration and NLR were determined. We found that ccfDNA levels were correlated with age and tumor burden. Moreover, higher values of NLR (≥3.31) were linked with worse overall survival (OS) (4 vs. 10 months; log rank
    MeSH term(s) Humans ; Neutrophils/pathology ; Prognosis ; Prospective Studies ; Lymphocyte Count ; Lymphocytes/pathology ; Pancreatic Neoplasms/pathology ; Carcinoma, Pancreatic Ductal/pathology ; Cell-Free Nucleic Acids ; Retrospective Studies
    Chemical Substances Cell-Free Nucleic Acids
    Language English
    Publishing date 2024-03-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25052854
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Endoscopic ultrasound-guided fine-needle aspiration pancreatic adenocarcinoma samples yield adequate DNA for next-generation sequencing: A cohort analysis.

    Bunduc, Stefania / Varzaru, Bianca / Iacob, Razvan Andrei / Sorop, Andrei / Manea, Ioana / Spiridon, Andreea / Chelaru, Raluca / Croitoru, Adina Emilia / Becheanu, Gabriel / Dumbrava, Mona / Dima, Simona / Popescu, Irinel / Gheorghe, Cristian

    World journal of gastroenterology

    2023  Volume 29, Issue 18, Page(s) 2864–2874

    Abstract: Background: Genetic tests are increasingly performed for the management of unresectable pancreatic cancer. For genotyping aimed samples current guidelines recommend using core specimens, although based on moderate quality evidence. However, in clinical ... ...

    Abstract Background: Genetic tests are increasingly performed for the management of unresectable pancreatic cancer. For genotyping aimed samples current guidelines recommend using core specimens, although based on moderate quality evidence. However, in clinical practice among the endoscopic ultrasound (EUS) guided tissue acquisition methods, fine needle aspiration (FNA) is the most widely performed.
    Aim: To assess the adequacy for next generation sequencing (NGS) of the DNA yielded from EUS-FNA pancreatic adenocarcinoma (PDAC) samples.
    Methods: Between November 2018 and December 2021, 105 patients with PDAC confirmed by EUS-FNA were included in the study at our tertiary gastroenterology center. Either 22 gauge (G) or 19G FNA needles were used. One pass was dedicated to DNA extraction. DNA concentration and purity (A260/280, A260/230) were assessed by spectrophotometry. We assessed the differences in DNA parameters according to needle size and tumor characteristics (size, location) and the adequacy of the extracted DNA for NGS (defined as A260/280 ≥ 1.7, and DNA yield: ≥ 10 ng for amplicon based NGS, ≥ 50 ng for whole exome sequencing [WES], ≥ 100 ng for whole genome sequencing [WGS]) by analysis of variance and
    Results: Our cohort included 49% male patients, aged 67.02 ± 8.38 years. The 22G needle was used in 71% of the cases. The DNA parameters across our samples varied as follows: DNA yield: 1289 ng (inter quartile range: 534.75-3101), A260/280 = 1.85 (1.79-1.86), A260/230 = 2.2 (1.72-2.36). DNA yield was > 10 ng in all samples and > 100 ng in 93% of them (one sample < 50 ng). There were no significant differences in the concentration and A260/280 between samples by needle size. Needle size was the only independent predictor of A260/230 which was higher in the 22G samples (
    Conclusion: EUS-FNA PDAC samples yield DNA adequate for subsequent NGS. DNA amount was similar between 22G and 19G FNA needles. DNA purity parameters may vary indirectly with needle size.
    MeSH term(s) Humans ; Male ; Female ; Pancreatic Neoplasms/diagnostic imaging ; Pancreatic Neoplasms/genetics ; Pancreas/pathology ; Endoscopic Ultrasound-Guided Fine Needle Aspiration/methods ; Adenocarcinoma/diagnostic imaging ; Adenocarcinoma/genetics ; Cohort Studies ; High-Throughput Nucleotide Sequencing ; Pancreatic Neoplasms
    Language English
    Publishing date 2023-06-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2185929-2
    ISSN 2219-2840 ; 1007-9327
    ISSN (online) 2219-2840
    ISSN 1007-9327
    DOI 10.3748/wjg.v29.i18.2864
    Database MEDical Literature Analysis and Retrieval System OnLINE

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