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Article: [Award for excellent poster presentation in the 60th Annual JSV Meeting].

Ikuta, Kazuyoshi

2014 Volume 63, Issue 1, Page(s) 87–88

MeSH term(s)	Awards and Prizes ; Humans ; Japan ; Posters as Topic ; Societies, Scientific/organization & administration ; Virology/organization & administration
Language	Japanese
Publishing date	2014-02-12
Publishing country	Japan
Document type	Journal Article
ZDB-ID	603272-2
ISSN	0042-6857
ISSN	0042-6857
DOI	10.2222/jsv.63.87
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Article: Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells.

Choa, John Benson D / Sasaki, Tadahiro / Kajiura, Hiroyuki / Ikuta, Kazuyoshi / Fujiyama, Kazuhito / Misaki, Ryo

Cytotechnology

2023 Volume 75, Issue 3, Page(s) 219–229

Abstract: Immunoglobulin A (IgA) has been showing potential as a new therapeutic antibody. However, recombinant IgA suffers from low yield. Supplementation of the medium is an effective approach to improving the production and quality of recombinant proteins. In ... ...

Abstract	Immunoglobulin A (IgA) has been showing potential as a new therapeutic antibody. However, recombinant IgA suffers from low yield. Supplementation of the medium is an effective approach to improving the production and quality of recombinant proteins. In this study, we adapted IgA1-producing CHO-K1 suspension cells to a high concentration (150 mM) of different disaccharides, namely sucrose, maltose, lactose, and trehalose, to improve the production and quality of recombinant IgA1. The disaccharide-adapted cell lines had slower cell growth rates, but their cell viability was extended compared to the nonadapted IgA1-producing cell line. Glucose consumption was exhausted in all cell lines except for the maltose-adapted one, which still contained glucose even after the 9th day of culturing. Lactate production was higher among the disaccharide-adapted cell lines. The specific productivity of the maltose-adapted IgA1-producing line was 4.5-fold that of the nonadapted line. In addition, this specific productivity was higher than in previous productions of recombinant IgA1 with a lambda chain. Lastly, secreted IgA1 aggregated in all cell lines, which may have been caused by self-aggregation. This aggregation was also found to begin inside the cells for maltose-adapted cell line. These results suggest that a high concentration of disaccharide-supplemented induced hyperosmolarity in the IgA1-producing CHO-K1 cell lines. In addition, the maltose-adapted CHO-K1 cell line benefited from having an additional source of carbohydrate. Supplementary information: The online version contains supplementary material available at 10.1007/s10616-023-00571-5.
Language	English
Publishing date	2023-03-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	1035772-5
ISSN	0920-9069
ISSN	0920-9069
DOI	10.1007/s10616-023-00571-5
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Article: Virus Neutralization by Human Intravenous Immunoglobulin Against Influenza Virus Subtypes A/H5 and A/H7.

Kubota-Koketsu, Ritsuko / Yunoki, Mikihiro / Okuno, Yoshinobu / Ikuta, Kazuyoshi

Biologics : targets & therapy

2021 Volume 15, Page(s) 87–94

Abstract: Purpose: Highly pathogenic avian influenza viruses are a threat to human health. Although donor populations have not experienced pandemic, they have been immunized by natural infections and/or vaccinations of influenza viruses such as A/H1N1, A/H3N2, ... ...

Abstract	Purpose: Highly pathogenic avian influenza viruses are a threat to human health. Although donor populations have not experienced pandemic, they have been immunized by natural infections and/or vaccinations of influenza viruses such as A/H1N1, A/H3N2, and B. Therefore, it is considered that human intravenous immunoglobulin (IVIG) derived from healthy donors does not include IgG against avian influenza viruses. However, cross-reactivity has not been evaluated yet. In this study, cross-reactivity against the avian influenza virus A/H5N1, A/H7N1, A/H7N2, A/H7N7, A/H7N9, and A/H10N9 was evaluated. Materials and methods: Several lots of IVIG derived from healthy donors in Japan were tested for virus neutralization using single- or multi-cycle virus neutralizing (S-VN or M-VN) assays that evaluate the infection-step associated with HA or the infection and propagation steps associated with HA and NA, respectively. In addition, anti-NA activities were evaluated by inhibiting the enzymatic activity in NAI assays. Results: IVIG lots showed high neutralizing activities against three A/H5N1 strains in M-VN assays, whereas activities in S-VN assays were unstable. In addition, A/H7N2 was also neutralized in S-VN and M-VN assays, with higher activity in M-VN than in S-VN assays. A/H7N1 was neutralized in S-VN and M-VN assays. In contrast, weak or no activity against A/H7N7, A/H7N9, and A/H10N9 was observed in S-VN and M-VN assays. NAI assay results show that IVIG lots had inhibitory activities against N1 and N2; however, N2 activities differed depending on the strain. In contrast, no activities were observed against N7 and N9. Conclusion: These results suggest that IVIG lots have neutralizing activity against avian influenza viruses during the virus propagation step, except for one strain, although no or weak activity was observed during the infection step.
Language	English
Publishing date	2021-04-13
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2415708-9
ISSN	1177-5491 ; 1177-5475
ISSN (online)	1177-5491
ISSN	1177-5475
DOI	10.2147/BTT.S291808
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Article: Next generation pneumococcal vaccine.

Akeda, Yukihiro / Ikuta, Kazuyoshi

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine

2017 Volume 104, Issue 11, Page(s) 2351–2356

MeSH term(s)	Humans ; Pneumococcal Vaccines
Chemical Substances	Pneumococcal Vaccines
Language	Japanese
Publishing date	2017-05-18
Publishing country	Japan
Document type	Journal Article
ZDB-ID	952816-7
ISSN	1883-2083 ; 0021-5384
ISSN (online)	1883-2083
ISSN	0021-5384
DOI	10.2169/naika.104.2351
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Article ; Online: Chimeric flavivirus causes vascular leakage and bone marrow suppression in a mouse model.

Kurosu, Takeshi / Hanabara, Keiko / Asai, Azusa / Pambudi, Sabar / Phanthanawiboon, Supranee / Omokoko, Magot Diata / Sakai, Yusuke / Suzuki, Tadaki / Ikuta, Kazuyoshi

Biochemical and biophysical research communications

2023 Volume 659, Page(s) 54–61

Abstract: Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the ... ...

Abstract	Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β-γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases.
MeSH term(s)	Mice ; Humans ; Animals ; Flavivirus ; Dengue Virus ; Dengue ; Bone Marrow/pathology ; Mice, Knockout ; Antibodies, Viral
Chemical Substances	Antibodies, Viral
Language	English
Publishing date	2023-04-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2023.04.003
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Article ; Online: Chimeric flavivirus causes vascular leakage and bone marrow suppression in a mouse model

Kurosu, Takeshi / Hanabara, Keiko / Asai, Azusa / Pambudi, Sabar / Phanthanawiboon, Supranee / Omokoko, Magot Diata / Sakai, Yusuke / Suzuki, Tadaki / Ikuta, Kazuyoshi

Biochemical and Biophysical Research Communications. 2023 June, v. 659 p.54-61

2023

Abstract	Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β–γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases.
Keywords	Japanese encephalitis virus ; bone marrow ; dengue ; humans ; intestines ; liver ; megakaryocytes ; mice ; models ; research ; viruses ; Dengue virus ; Flavivirus ; Mouse model ; Bone marrow suppression ; Vascular leakage ; Hemorrhagic fever
Language	English
Dates of publication	2023-06
Size	p. 54-61.
Publishing place	Elsevier Inc.
Document type	Article ; Online
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2023.04.003
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Article ; Online: Two Different Strains of Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) in North and South Osaka by Phylogenetic Analysis of Evolutionary Lineage: Evidence for Independent SFTSV Transmission.

Ikemori, Ryo / Aoyama, Ikuko / Sasaki, Tadahiro / Takabayashi, Hirono / Morisada, Kazutoshi / Kinoshita, Masaru / Ikuta, Kazuyoshi / Yumisashi, Takahiro / Motomura, Kazushi

Viruses

2021 Volume 13, Issue 2

Abstract: Severe fever with thrombocytopenia syndrome (SFTS) is a novel tick-borne infectious disease, therefore, the information on the whole genome of the SFTS virus (SFTSV) is still limited. This study demonstrates a nearly whole genome of the SFTSV identified ... ...

Abstract	Severe fever with thrombocytopenia syndrome (SFTS) is a novel tick-borne infectious disease, therefore, the information on the whole genome of the SFTS virus (SFTSV) is still limited. This study demonstrates a nearly whole genome of the SFTSV identified in Osaka in 2017 and 2018 by next-generation sequencing (NGS). The evolutionary lineage of two genotypes, C5 and J1, was identified in Osaka. The first case in Osaka belongs to suspect reassortment (L:C5, M:C5, S:C4), the other is genotype J1 (L: J1, M: J1, S: J1) according to the classification by a Japanese group. C5 was identified in China, indicating that C5 identified in this study may be transmitted by birds between China and Japan. This study revealed that different SFTSV genotypes were distributed in two local areas, suggesting the separate or focal transmission patterns in Osaka.
MeSH term(s)	Evolution, Molecular ; Genome, Viral/genetics ; Genotype ; Humans ; Japan ; Phlebovirus/classification ; Phlebovirus/genetics ; Phlebovirus/isolation & purification ; Phylogeny ; RNA, Viral/genetics ; Severe Fever with Thrombocytopenia Syndrome/virology
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-01-25
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v13020177
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Article ; Online: Polyethyleneimine-modified resins effectively remove porcine circovirus and cellular prion protein.

Yunoki, Mikihiro / Urayama, Takeru / Aoyama, Shigeyuki / Okaniwa, Natsuki / Sakai, Kaoru / Uchida, Eriko / Ikuta, Kazuyoshi / Yamaguchi, Teruhide

Journal of virological methods

2021 Volume 294, Page(s) 114181

Abstract: Polyethyleneimine (PEI) possesses various molecular weights (MWs), structures, and virus capture capacities. However, whether PEI can capture porcine circovirus (PCV) and animal cell-derived prion protein ( ... ...

Abstract	Polyethyleneimine (PEI) possesses various molecular weights (MWs), structures, and virus capture capacities. However, whether PEI can capture porcine circovirus (PCV) and animal cell-derived prion protein (PrP
MeSH term(s)	Animals ; Circovirus ; Polyethyleneimine ; Prion Proteins ; Swine
Chemical Substances	Prion Proteins ; Polyethyleneimine (9002-98-6)
Language	English
Publishing date	2021-05-10
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2021.114181
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Article ; Online: Capturing and concentrating adenovirus using magnetic anionic nanobeads.

Sakudo, Akikazu / Baba, Koichi / Ikuta, Kazuyoshi

International journal of nanomedicine

2016 Volume 11, Page(s) 1847–1857

Abstract: We recently demonstrated how various enveloped viruses can be efficiently concentrated using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). However, the exact mechanism of interaction between the virus particles ...

Abstract	We recently demonstrated how various enveloped viruses can be efficiently concentrated using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). However, the exact mechanism of interaction between the virus particles and anionic beads remains unclear. To further investigate whether these magnetic anionic beads specifically bind to the viral envelope, we examined their potential interaction with a nonenveloped virus (adenovirus). The beads were incubated with either adenovirus-infected cell culture medium or nasal aspirates from adenovirus-infected individuals and then separated from the supernatant by applying a magnetic field. After thoroughly washing the beads, adsorption of adenovirus was confirmed by a variety of techniques, including immunochromatography, polymerase chain reaction, Western blotting, and cell culture infection assays. These detection methods positively identified the hexon and penton capsid proteins of adenovirus along with the viral genome on the magnetic beads. Furthermore, various types of adenovirus including Types 5, 6, 11, 19, and 41 were captured using the magnetic bead procedure. Our bead capture method was also found to increase the sensitivity of viral detection. Adenovirus below the detectable limit for immunochromatography was efficiently concentrated using the magnetic bead procedure, allowing the virus to be successfully detected using this methodology. Moreover, these findings clearly demonstrate that a viral envelope is not required for binding to the anionic magnetic beads. Taken together, our results show that this capture procedure increases the sensitivity of detection of adenovirus and would, therefore, be a valuable tool for analyzing both clinical and experimental samples.
MeSH term(s)	Adenoviridae/genetics ; Adenoviridae/isolation & purification ; Anions ; Blotting, Western ; Humans ; Immunomagnetic Separation/methods ; Limit of Detection ; Magnetics ; Maleates/chemistry ; Nanostructures ; Nose/virology ; Polyethylenes/chemistry ; Polymerase Chain Reaction ; Polymers/chemistry
Chemical Substances	Anions ; Maleates ; Polyethylenes ; Polymers ; poly(methyl vinyl ether-co-maleic anhydride) (9011-16-9)
Language	English
Publishing date	2016-05-09
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2364941-0
ISSN	1178-2013 ; 1176-9114
ISSN (online)	1178-2013
ISSN	1176-9114
DOI	10.2147/IJN.S104926
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Article ; Online: A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads.

Sakudo, Akikazu / Ikuta, Kazuyoshi

International journal of molecular medicine

2012 Volume 30, Issue 2, Page(s) 437–442

Abstract: Magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate) [poly(MVE-MA)], were used in a method to capture human immunodeficiency virus type-1 (HIV-1). The beads were incubated with either HIV-1- ... ...

Abstract	Magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate) [poly(MVE-MA)], were used in a method to capture human immunodeficiency virus type-1 (HIV-1). The beads were incubated with either HIV-1-infected cell culture medium or plasma from HIV-1 infected individuals and separated from the supernatant by applying a magnetic field. After thorough washing, adsorption of HIV-1 by the beads was confirmed by reverse transcription (RT)-polymerase chain reaction (PCR), real-time PCR, enzyme-linked immunosorbent assay and western blotting. The results confirmed the presence of envelope, polymerase, Nef and the viral genome of HIV-1. Furthermore, various subtypes and circulating recombinant forms (CRFs) of HIV-1 including subtype B, C and CRF01_AE and the immature form of subtype B HIV-1 could be captured. Preincubation with neutralizing antibody against HIV-1 envelope gp41 decreased the capture efficiently, suggesting that poly(MVE-MA) binds HIV-1 via gp41. We believe that this capture procedure will be a valuable tool for detecting various types of HIV-1 in both clinical and experimental samples.
MeSH term(s)	Anions ; Cell Line ; Enzyme-Linked Immunosorbent Assay/methods ; HIV Infections/diagnosis ; HIV Infections/virology ; HIV-1/genetics ; HIV-1/isolation & purification ; Humans ; Magnetics ; Polymers ; Reassortant Viruses/genetics ; Reassortant Viruses/isolation & purification ; Recombination, Genetic ; Reverse Transcriptase Polymerase Chain Reaction
Chemical Substances	Anions ; Polymers
Language	English
Publishing date	2012-08
Publishing country	Greece
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1444428-8
ISSN	1791-244X ; 1107-3756
ISSN (online)	1791-244X
ISSN	1107-3756
DOI	10.3892/ijmm.2012.1009
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