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  1. Article ; Online: Droplet-Based Cytotoxicity Assay

    Silvia Antona / Ilia Platzman / Joachim P. Spatz

    ACS Omega, Vol 5, Iss 38, Pp 24674-

    Implementation of Time-Efficient Screening of Antitumor Activity of Natural Killer Cells

    2020  Volume 24683

    Keywords Chemistry ; QD1-999
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher American Chemical Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Bottom-up assembly of viral replication cycles

    Oskar Staufer / Gösta Gantner / Ilia Platzman / Klaus Tanner / Imre Berger / Joachim P. Spatz

    Nature Communications, Vol 13, Iss 1, Pp 1-

    2022  Volume 10

    Abstract: Synthetic biology encompasses engineering approaches to either redesign biological organisms with new molecular abilities or build up organism from the bottom to improve our understanding of life. Here, the authors summarize current approaches that can ... ...

    Abstract Synthetic biology encompasses engineering approaches to either redesign biological organisms with new molecular abilities or build up organism from the bottom to improve our understanding of life. Here, the authors summarize current approaches that can be applied for the synthetic engineering of viral replication cycles and discuss the potential DURC risk associated with such bottom-up approaches in infectious disease.
    Keywords Science ; Q
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Autonomous Directional Motion of Actin‐Containing Cell‐Sized Droplets

    Barbara Haller / Kevin Jahnke / Marian Weiss / Kerstin Göpfrich / Ilia Platzman / Joachim Pius Spatz

    Advanced Intelligent Systems, Vol 3, Iss 5, Pp n/a-n/a (2021)

    2021  

    Abstract: Cell motility is potentially the most apparent distinction of living matter, serving an essential purpose in single cells and multicellular organisms alike. Thus, the bottom‐up reconstitution of autonomous motion of cell‐sized compartments remains an ... ...

    Abstract Cell motility is potentially the most apparent distinction of living matter, serving an essential purpose in single cells and multicellular organisms alike. Thus, the bottom‐up reconstitution of autonomous motion of cell‐sized compartments remains an exciting but challenging goal. Herein, actin‐driven Marangoni flows are engineered to generate rotational and translational motility of surfactant‐stabilized emulsion droplets. The interaction between actin filaments and the negatively charged block‐copolymer Krytox is identified as the driving force for Marangoni flows at the droplet interface. Tuning the actin–Krytox interplay, sustained autonomous unidirectional droplet rotation with 1.7 rot h−1 is achieved. Ultimately, this rotational motion is transformed into a translational rolling motion by introducing interactions between the droplets and the surface of the observation chamber. Accordingly, translational motility of actin‐containing droplets at velocities of 0.061 ± 0.014 μm s−1 is reported herein and an overall displacement of several hundreds of micrometers within 30 min is observed. These self‐propelled systems with biologically active molecules demonstrate how motility could be implemented for synthetic cells.
    Keywords actin ; droplet-based microfluidics ; Marangoni effect ; motility ; surfactants ; synthetic biology ; Computer engineering. Computer hardware ; TK7885-7895 ; Control engineering systems. Automatic machinery (General) ; TJ212-225
    Subject code 629
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Vesicle Induced Receptor Sequestration

    Oskar Staufer / Jochen Estebano Hernandez Bücher / Julius Fichtler / Martin Schröter / Ilia Platzman / Joachim P. Spatz

    Advanced Science, Vol 9, Iss 13, Pp n/a-n/a (2022)

    Mechanisms behind Extracellular Vesicle‐Based Protein Signaling

    2022  

    Abstract: Abstract Extracellular vesicles (EVs) are fundamental for proper physiological functioning of multicellular organisms. By shuttling nucleic acids and proteins between cells, EVs regulate a plethora of cellular processes, especially those involved in ... ...

    Abstract Abstract Extracellular vesicles (EVs) are fundamental for proper physiological functioning of multicellular organisms. By shuttling nucleic acids and proteins between cells, EVs regulate a plethora of cellular processes, especially those involved in immune signalling. However, the mechanistic understanding concerning the biophysical principles underlying EV‐based communication is still incomplete. Towards holistic understanding, particular mechanisms explaining why and when cells apply EV‐based communication and how protein‐based signalling is promoted by EV surfaces are sought. Here, the authors study vesicle‐induced receptor sequestration (VIRS) as a universal mechanism augmenting the signalling potency of proteins presented on EV‐membranes. By bottom‐up reconstitution of synthetic EVs, the authors show that immobilization of the receptor ligands FasL and RANK on EV‐like vesicles, increases their signalling potential by more than 100‐fold compared to their soluble forms. Moreover, the authors perform diffusion simulations within immunological synapses to compare receptor activation between soluble and EV‐presented proteins. By this the authors propose vesicle‐triggered local clustering of membrane receptors as the principle structural mechanism underlying EV‐based protein presentation. The authors conclude that EVs act as extracellular templates promoting the local aggregation of membrane receptors at the EV contact site, thereby fostering inter‐protein interactions. The results uncover a potentially universal mechanism explaining the unique structural profit of EV‐based intercellular signalling.
    Keywords bottom‐up synthetic biology ; CD95 ; ectosomes ; Fas ; FasL ; immunological synapse ; Science ; Q
    Subject code 612
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Electrocoalescence of Water-in-Oil Droplets with a Continuous Aqueous Phase

    Christoph Frey / Kerstin Göpfrich / Sadaf Pashapour / Ilia Platzman / Joachim P. Spatz

    ACS Omega, Vol 5, Iss 13, Pp 7529-

    Implementation of Controlled Content Release

    2020  Volume 7536

    Keywords Chemistry ; QD1-999
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher American Chemical Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Synthetic cells with self-activating optogenetic proteins communicate with natural cells

    Omer Adir / Mia R. Albalak / Ravit Abel / Lucien E. Weiss / Gal Chen / Amit Gruber / Oskar Staufer / Yaniv Kurman / Ido Kaminer / Jeny Shklover / Janna Shainsky-Roitman / Ilia Platzman / Lior Gepstein / Yoav Shechtman / Benjamin A. Horwitz / Avi Schroeder

    Nature Communications, Vol 13, Iss 1, Pp 1-

    2022  Volume 13

    Abstract: Synthetic biology and engineering approaches are harnessed to incorporate new capabilities in synthetic cells. Here, the authors designed bioluminescent signaling mechanisms for intracellular and intercellular synthetic-to-natural cell communication. ...

    Abstract Synthetic biology and engineering approaches are harnessed to incorporate new capabilities in synthetic cells. Here, the authors designed bioluminescent signaling mechanisms for intracellular and intercellular synthetic-to-natural cell communication.
    Keywords Science ; Q
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoprotein

    Oskar Staufer / Kapil Gupta / Jochen Estebano Hernandez Bücher / Fabian Kohler / Christian Sigl / Gunjita Singh / Kate Vasileiou / Ana Yagüe Relimpio / Meline Macher / Sebastian Fabritz / Hendrik Dietz / Elisabetta Ada Cavalcanti Adam / Christiane Schaffitzel / Alessia Ruggieri / Ilia Platzman / Imre Berger / Joachim P. Spatz

    Nature Communications, Vol 13, Iss 1, Pp 1-

    2022  Volume 13

    Abstract: Staufer et al. provide a protocol for preparation of synthetic minimal virions (MiniV) of SARS-CoV-2, mimicking viral structure and allowing for precise investigation of receptor binding mechanism. They find that the highly conserved free fatty acid ... ...

    Abstract Staufer et al. provide a protocol for preparation of synthetic minimal virions (MiniV) of SARS-CoV-2, mimicking viral structure and allowing for precise investigation of receptor binding mechanism. They find that the highly conserved free fatty acid binding pocket (FABP) can function as an allosteric regulator, enabling adaptation of immunogenicity via binding of proinflammatory free fatty acids and mediating the spike open to-closed equilibrium.
    Keywords Science ; Q
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells

    Kevin Jahnke / Noah Ritzmann / Julius Fichtler / Anna Nitschke / Yannik Dreher / Tobias Abele / Götz Hofhaus / Ilia Platzman / Rasmus R. Schröder / Daniel J. Müller / Joachim P. Spatz / Kerstin Göpfrich

    Nature Communications, Vol 12, Iss 1, Pp 1-

    2021  Volume 9

    Abstract: Controlled actuation is an important aspect of synthetic cellular systems. Here, the authors combine pH responsive DNA origami structures with light triggered proton pump engineered E. coli to trigger a change in pH and control the deformation of giant ... ...

    Abstract Controlled actuation is an important aspect of synthetic cellular systems. Here, the authors combine pH responsive DNA origami structures with light triggered proton pump engineered E. coli to trigger a change in pH and control the deformation of giant unilamellar vesicles by simple illumination.
    Keywords Science ; Q
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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