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  1. Article ; Online: Effect of Lactate Export Inhibition on Anaplastic Thyroid Cancer Growth and Metabolism.

    Zhao, Bixiao / Aggarwal, Abha / Im, Soo-Yeon / Viswanathan, Keya / Landa, Iñigo / Nehs, Matthew A

    Journal of the American College of Surgeons

    2022  Volume 234, Issue 6, Page(s) 1044–1050

    Abstract: Background: Anaplastic thyroid cancer (ATC) is an aggressive malignancy without effective treatments. ATC cells demonstrate upregulated glycolysis (Warburg effect), generating lactate that is subsequently exported by monocarboxylate transporter 4 (MCT4). ...

    Abstract Background: Anaplastic thyroid cancer (ATC) is an aggressive malignancy without effective treatments. ATC cells demonstrate upregulated glycolysis (Warburg effect), generating lactate that is subsequently exported by monocarboxylate transporter 4 (MCT4). This study aims to determine whether MCT4 inhibition can suppress ATC growth.
    Study design: ATC cell lines 8505C, JL30, and TCO1 were grown in low (3 mmol/L; LG) or high (25 mmol/L; HG) glucose medium containing the lactate shuttle inhibitors acriflavine (10-25 μmol/L; ACF), syrosingopine (100 µmol/L; SYR), or AZD3965 (20 µmol/L; AZD). Lactate level and cell proliferation were measured with standard assays. Seahorse analysis was performed to determine glycolytic response.
    Results: Compared with HG, addition of ACF to LG decreased lactate secretion for both 8505C (p < 10-5) and JL30 (p < 10-4) cells, whereas proliferation was also reduced (p < 10-4 and 10-5, respectively). During Seahorse analysis, addition of oligomycin increased acidification by 84 mpH/min in HG vs 10 mpH/min in LG containing ACF (p < 10-5). Treatment with LG and SYR drastically diminished 8505C and TCO1 growth vs HG (p < 0.01 for both). LG and AZD treatment also led to reduced proliferation in tested cell lines (p ≤ 0.01 for all) that was further decreased by addition of ACF (p < 10-4 vs HG, p ≤ 0.01 vs LG and AZD).
    Conclusion: Inhibition of lactate shuttles significantly reduced proliferation and glycolytic capacity of ATC cells in a low-glucose environment. Targeting suppression of glycolytic and lactate processing pathways may represent an effective treatment strategy for ATC.
    MeSH term(s) Cell Line, Tumor ; Glucose/metabolism ; Humans ; Lactic Acid/metabolism ; Thyroid Carcinoma, Anaplastic/drug therapy ; Thyroid Carcinoma, Anaplastic/metabolism ; Thyroid Carcinoma, Anaplastic/pathology ; Thyroid Neoplasms/drug therapy ; Thyroid Neoplasms/pathology
    Chemical Substances Lactic Acid (33X04XA5AT) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2022-04-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1181115-8
    ISSN 1879-1190 ; 1072-7515
    ISSN (online) 1879-1190
    ISSN 1072-7515
    DOI 10.1097/XCS.0000000000000226
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Clove oil as an anaesthetic for common octopus (Octopus minor, Sasaki)

    Seol, Dong-Won / Lee, Jinhwan / Im, Soo-Yeon / Park, In-Seok

    Aquaculture research. 2007 Jan., v. 38, no. 1

    2007  

    Abstract: We evaluated the anaesthetic effect of clove oil [2-methoxy-4-2-(2-propenyl)-phenol] on the common octopus (Octopus minor), in terms of the time required to become anaesthetized ('anaesthetic time') and recovery time. We used a factorial experimental ... ...

    Abstract We evaluated the anaesthetic effect of clove oil [2-methoxy-4-2-(2-propenyl)-phenol] on the common octopus (Octopus minor), in terms of the time required to become anaesthetized ('anaesthetic time') and recovery time. We used a factorial experimental design and administered clove oil at different temperatures (15, 20 and 25°C) and concentrations (50, 100, 150, 200, 250 and 300 mg L⁻¹). We observed a significant (P<0.05) relationship between concentration and temperature, and each variable was effective (P<0.05). Anaesthetic time linearly decreased as the concentration and temperature increased. However, recovery time increased as the concentration increased and temperature decreased. There was no mortality. A concentration of 200 mg L⁻¹ clove oil showed rapid anaesthetic and recovery times in the common octopus, indicating its suitability for this species.
    Keywords Octopodidae ; anesthetics ; cloves ; essential oils ; experimental design ; mortality ; oils ; temperature
    Language English
    Dates of publication 2007-01
    Size p. 45-49.
    Publisher Blackwell Publishing Ltd
    Publishing place Oxford, UK
    Document type Article
    ZDB-ID 1227359-4
    ISSN 1355-557X
    ISSN 1355-557X
    DOI 10.1111/j.1365-2109.2006.01622.x
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  3. Article: Anaesthetic efficacy and physiological responses to clove oil-anaesthetized kelp grouper Epinephelus bruneus

    Park, Min Ouk / Hur, Woo June / Im, Soo-Yeon / Seol, Dong-Won / Lee, Jinhwan / Park, In-Seok

    Aquaculture research. 2008 June, v. 39, no. 8

    2008  

    Abstract: The efficacy of clove oil as an anaesthetic and at producing a physiological response (plasma cortisol and glucose) was evaluated in the kelp grouper, Epinephelus bruneus. To acquire complete anaesthesia in less than 3 min and recovery in <10 min, three ... ...

    Abstract The efficacy of clove oil as an anaesthetic and at producing a physiological response (plasma cortisol and glucose) was evaluated in the kelp grouper, Epinephelus bruneus. To acquire complete anaesthesia in less than 3 min and recovery in <10 min, three doses of clove oil were tested at 18, 22 and 26 °C. Although higher anaesthetic doses resulted in shorter induction times and longer recovery times, and a lower temperature resulted in longer anaesthesia induction and slower recovery, we found the optimal dose and administering temperature of clove oil to be 250-300 mg L⁻¹ at water temperature of 18 °C, 150-200 mg L⁻¹ at water temperature of 22 °C and 50-100 mg L⁻¹ at water temperature of 26 °C respectively. Following the administration of 150 mg L⁻¹ of clove oil at 22 °C, the plasma cortisol level was highest (4.24 ± 1.571 μg dL⁻¹) after 12 h and the plasma glucose was highest (92.7 ± 9.61 mg dL⁻¹) after 2 h. These results should be useful to the aquaculture industry, where anaesthesia is necessary for a range of activities.
    Keywords anesthesia
    Language English
    Dates of publication 2008-06
    Size p. 877-884.
    Publisher Blackwell Publishing Ltd
    Publishing place Oxford, UK
    Document type Article
    ZDB-ID 1227359-4
    ISSN 1355-557X
    ISSN 1355-557X
    DOI 10.1111/j.1365-2109.2008.01941.x
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  4. Article ; Online: Preclinical efficacy of daratumumab in T-cell acute lymphoblastic leukemia.

    Bride, Karen L / Vincent, Tiffaney L / Im, Soo-Yeon / Aplenc, Richard / Barrett, David M / Carroll, William L / Carson, Robin / Dai, Yunfeng / Devidas, Meenakshi / Dunsmore, Kimberly P / Fuller, Tori / Glisovic-Aplenc, Tina / Horton, Terzah M / Hunger, Stephen P / Loh, Mignon L / Maude, Shannon L / Raetz, Elizabeth A / Winter, Stuart S / Grupp, Stephan A /
    Hermiston, Michelle L / Wood, Brent L / Teachey, David T

    Blood

    2018  Volume 131, Issue 9, Page(s) 995–999

    Abstract: As a consequence of acquired or intrinsic disease resistance, the prognosis for patients with relapsed or refractory T-cell acute lymphoblastic leukemia (T-ALL) is dismal. Novel, less toxic drugs are clearly needed. One of the most promising emerging ... ...

    Abstract As a consequence of acquired or intrinsic disease resistance, the prognosis for patients with relapsed or refractory T-cell acute lymphoblastic leukemia (T-ALL) is dismal. Novel, less toxic drugs are clearly needed. One of the most promising emerging therapeutic strategies for cancer treatment is targeted immunotherapy. Immune therapies have improved outcomes for patients with other hematologic malignancies including B-cell ALL; however no immune therapy has been successfully developed for T-ALL. We hypothesize targeting CD38 will be effective against T-ALL. We demonstrate that blasts from patients with T-ALL have robust surface CD38 surface expression and that this expression remains stable after exposure to multiagent chemotherapy. CD38 is expressed at very low levels on normal lymphoid and myeloid cells and on a few tissues of nonhematopoietic origin, suggesting that CD38 may be an ideal target. Daratumumab is a human immunoglobulin G1κ monoclonal antibody that binds CD38, and has been demonstrated to be safe and effective in patients with refractory multiple myeloma. We tested daratumumab in a large panel of T-ALL patient-derived xenografts (PDX) and found striking efficacy in 14 of 15 different PDX. These data suggest that daratumumab is a promising novel therapy for pediatric T-ALL patients.
    MeSH term(s) ADP-ribosyl Cyclase 1/antagonists & inhibitors ; ADP-ribosyl Cyclase 1/metabolism ; Adolescent ; Adult ; Animals ; Antibodies, Monoclonal/adverse effects ; Antibodies, Monoclonal/pharmacology ; Child ; Child, Preschool ; Female ; Gene Expression Regulation, Leukemic/drug effects ; Humans ; Male ; Membrane Glycoproteins/antagonists & inhibitors ; Membrane Glycoproteins/metabolism ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Neoplasm Proteins/antagonists & inhibitors ; Neoplasm Proteins/metabolism ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/drug therapy ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/metabolism ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/pathology ; Xenograft Model Antitumor Assays
    Chemical Substances Antibodies, Monoclonal ; Membrane Glycoproteins ; Neoplasm Proteins ; daratumumab (4Z63YK6E0E) ; CD38 protein, human (EC 3.2.2.5) ; ADP-ribosyl Cyclase 1 (EC 3.2.2.6)
    Language English
    Publishing date 2018-01-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80069-7
    ISSN 1528-0020 ; 0006-4971
    ISSN (online) 1528-0020
    ISSN 0006-4971
    DOI 10.1182/blood-2017-07-794214
    Database MEDical Literature Analysis and Retrieval System OnLINE

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