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  1. Article ; Online: Fluorescence Spectroscopy Based Identification of Pseudomonas Aeruginosa and Escherichia Coli Suspensions.

    Imtiaz, Sana / Saleem, Muhammad

    Journal of fluorescence

    2024  

    Abstract: In this article, Fluorescence spectroscopy has been employed for the identification of Pseudomonas aeruginosa (PA) and Escherichia coli (E. coli) in water suspension. Emission spectra of PA and E. coli suspensions have been acquired by using excitation ... ...

    Abstract In this article, Fluorescence spectroscopy has been employed for the identification of Pseudomonas aeruginosa (PA) and Escherichia coli (E. coli) in water suspension. Emission spectra of PA and E. coli suspensions have been acquired by using excitation wavelengths from 270 to 420 nm with steps of 10 nm to explore their spectral features. It has been found that the emission spectra of tryptophan, tyrosine, NADH and FAD, being the intracellular biomolecules present in both bacteria, can be used as fingerprints for their identification, differentiation and quantification. Both bacterial strains can clearly be differentiated from water and from each other by using λ
    Language English
    Publishing date 2024-02-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2016892-5
    ISSN 1573-4994 ; 1053-0509
    ISSN (online) 1573-4994
    ISSN 1053-0509
    DOI 10.1007/s10895-024-03608-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Fluorescence Spectroscopy Based Characterization of Pseudomonas Aeruginosa Suspension.

    Imtiaz, Sana / Saleem, Muhammad

    Journal of fluorescence

    2023  

    Abstract: In this article, optical characterization of Pseudomonas aeruginosa (PA) suspension has been performed by using Fluorescence spectroscopy. Optical density (OD) and plate count methods have been employed as a reference for the analysis of emission spectra ...

    Abstract In this article, optical characterization of Pseudomonas aeruginosa (PA) suspension has been performed by using Fluorescence spectroscopy. Optical density (OD) and plate count methods have been employed as a reference for the analysis of emission spectra of Pseudomonas aeruginosa in water suspension. Emission spectra of PA suspension has been acquired by using excitation wavelengths from 270 to 420 nm with step of 10 nm to explore its spectral behavior. It has been found that emission spectra of tryptophan, tyrosine, NADH and FAD, the intracellular biomolecules of bacteria, can be used as finger prints for the detection of Pseudomonas aeruginosa. Furthermore, the effect of water matrix on the spectral emission of Pseudomonas aeruginosa has been investigated that might be one of the limitation of Fluorescence spectroscopy for complex water matrices. Moreover, a calibration curve has been produced between ODs
    Language English
    Publishing date 2023-09-15
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2016892-5
    ISSN 1573-4994 ; 1053-0509
    ISSN (online) 1573-4994
    ISSN 1053-0509
    DOI 10.1007/s10895-023-03436-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: In vitro study: methylene blue-based antibacterial photodynamic inactivation of Pseudomonas aeruginosa.

    Zada, Laiq / Anwar, Shahzad / Imtiaz, Sana / Saleem, Muhammad / Shah, Aamer Ali

    Applied microbiology and biotechnology

    2024  Volume 108, Issue 1, Page(s) 169

    Abstract: Pseudomonas aeruginosa is one of the most antibiotic-resistant and opportunistic pathogens in immunocompromised and debilitated patients. It is considered the cause of most severe skin infections and is frequently found in hospital burn units. Due to its ...

    Abstract Pseudomonas aeruginosa is one of the most antibiotic-resistant and opportunistic pathogens in immunocompromised and debilitated patients. It is considered the cause of most severe skin infections and is frequently found in hospital burn units. Due to its high antibiotic resistance, eliminating P. aeruginosa from skin infections is quite challenging. Therefore, this study aims to assess the novel in vitro antibacterial activity of methylene blue using a 635-nm diode laser to determine the effective power and energy densities for inhibition of P. aeruginosa. The strain was treated with various concentrations of methylene blue and 635-nm diode laser at powers of 300 mW/cm
    MeSH term(s) Humans ; Pseudomonas aeruginosa ; Methylene Blue/pharmacology ; Pseudomonas Infections ; Anti-Bacterial Agents/pharmacology ; Immunocompromised Host
    Chemical Substances Methylene Blue (T42P99266K) ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-01-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-024-13009-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Effective Treatment of Solitary Rectal Ulcer Syndrome by Using Photobiomodulation Therapy: A Case Report.

    Imtiaz, Sana / Akhter, Tayyab Saeed / Bushra, Hamama Tul / Khan, Javeria Zahid / Niazi, Aasiya / Saleem, Muhammad / Umar, Muhammad

    Photobiomodulation, photomedicine, and laser surgery

    2024  Volume 42, Issue 2, Page(s) 182–185

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Female ; Humans ; Low-Level Light Therapy ; Rectal Diseases/therapy ; Rectal Diseases/diagnosis ; Rectal Diseases/pathology ; Syndrome ; Treatment Outcome ; Ulcer/radiotherapy ; Ulcer/diagnosis ; Adult
    Language English
    Publishing date 2024-02-02
    Publishing country United States
    Document type Case Reports ; Journal Article
    ISSN 2578-5478
    ISSN (online) 2578-5478
    DOI 10.1089/photob.2023.0117
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Fluorescence Spectroscopy for the Assessment of Microbial Load in UVC Treated Water.

    Imtiaz, Sana / Anwar, Shahzad / Zada, Laiq / Ali, Hina / Khurram, M Saeed / Saeed, Azhar / Saleem, Muhammad

    Journal of fluorescence

    2023  Volume 33, Issue 6, Page(s) 2339–2347

    Abstract: In this article, Fluorescence spectroscopy has been employed for the assessment of microbial load and it has been compared with the gold standard colony forming unit (CFU) and optical density (OD) methods. In order to develop a correlation between three ... ...

    Abstract In this article, Fluorescence spectroscopy has been employed for the assessment of microbial load and it has been compared with the gold standard colony forming unit (CFU) and optical density (OD) methods. In order to develop a correlation between three characterization techniques, water samples of different microbial loads have been prepared by UVC disinfection method through an indigenously developed NUVWater sterilizer, which operates in close cycle flow configuration. A UV dose of 58.9 mJ/cm
    MeSH term(s) Spectrometry, Fluorescence ; Ultraviolet Rays ; Bacteria ; Drinking Water ; Disinfection/methods
    Chemical Substances Drinking Water
    Language English
    Publishing date 2023-04-12
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2016892-5
    ISSN 1573-4994 ; 1053-0509
    ISSN (online) 1573-4994
    ISSN 1053-0509
    DOI 10.1007/s10895-023-03226-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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