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Article ; Online: Instrumentation development, improvement, simplification, and miniaturization: The multifunctional plate source for use in mass spectrometry.

Trimpin, Sarah / Inutan, Ellen / Coffinberger, Hope / Hoang, Khoa / Yenchick, Frank / Wager-Miller, James / Pophristic, Milan / Mackie, Ken / McEwen, Charles N

European journal of mass spectrometry (Chichester, England)

2023 Volume 29, Issue 5-6, Page(s) 276–291

Abstract: In remembrance of Prof. Dr Przybylski, we are presenting a vision towards his beloved mass spectrometry (MS) and its far-reaching promises outside of the academic laboratory. Sub-atmospheric pressure (AP) ionization MS is well positioned to make a step- ... ...

Abstract	In remembrance of Prof. Dr Przybylski, we are presenting a vision towards his beloved mass spectrometry (MS) and its far-reaching promises outside of the academic laboratory. Sub-atmospheric pressure (AP) ionization MS is well positioned to make a step-change in direct ionization, a concept that allows
Language	English
Publishing date	2023-11-24
Publishing country	England
Document type	Journal Article
ISSN	1751-6838
ISSN (online)	1751-6838
DOI	10.1177/14690667231211486
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Article: Expression and In Vivo Characterization of the Antimicrobial Peptide Oncocin and Variants Binding to Ribosomes

Muthunayake, Nisansala S / Islam, Rabiul / Inutan, Ellen D / Colangelo, Wesley / Trimpin, Sarah / Cunningham, Philip R / Chow, Christine S

Biochemistry. 2020 Aug. 25, v. 59, no. 36

2020

Abstract: Peptides have important biomedical applications, but poor correlation between in vitro and in vivo activities can limit their development for clinical use. The ability to generate peptides and monitor their expression with new mass spectrometric methods ... ...

Abstract	Peptides have important biomedical applications, but poor correlation between in vitro and in vivo activities can limit their development for clinical use. The ability to generate peptides and monitor their expression with new mass spectrometric methods and biological activities in vivo would be an advantage for the discovery and improvement of peptide-based drugs. In this study, a plasmid-based system was used to express the ribosome-targeting peptide oncocin (19 amino acids, VDKPPYLPRPRPPRRIYNR) and to determine its direct antibacterial effects on Escherichia coli. Previous biochemical and structure studies showed that oncocin targets the bacterial ribosome. The oncocin peptide generated in vivo strongly inhibits bacterial growth. In vivo dimethyl sulfate footprinting of oncocin on the rRNA gives results that are consistent with those of in vitro studies but reveals additional binding interactions with E. coli ribosomes. Furthermore, expression of truncated or mutated peptides reveals which amino acids are important for antimicrobial activity. Overall, the in vivo peptide expression system can be used to investigate biological activities and interactions of peptides with their targets within the cellular environment and to separate contributions of the sequence to cellular transport. This strategy has future applications for improving the effectiveness of existing peptides and developing new peptide-based drugs.
Keywords	Escherichia coli ; amino acids ; antibacterial properties ; antimicrobial peptides ; bacterial growth ; correlation ; dimethyl sulfate ; drugs ; in vitro studies ; mass spectrometry ; ribosomes
Language	English
Dates of publication	2020-0825
Size	p. 3380-3391.
Publishing place	American Chemical Society
Document type	Article
Note	NAL-light
ZDB-ID	1108-3
ISSN	1520-4995 ; 0006-2960
ISSN (online)	1520-4995
ISSN	0006-2960
DOI	10.1021/acs.biochem.0c00600
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Article ; Online: Matrix assisted ionization in vacuum, a sensitive and widely applicable ionization method for mass spectrometry.

Trimpin, Sarah / Inutan, Ellen D

Journal of the American Society for Mass Spectrometry

2013 Volume 24, Issue 5, Page(s) 722–732

Abstract: An astonishingly simple new method to produce gas-phase ions of small molecules as well as proteins from the solid state under cold vacuum conditions is described. This matrix assisted ionization vacuum (MAIV) mass spectrometry (MS) method produces ... ...

Abstract	An astonishingly simple new method to produce gas-phase ions of small molecules as well as proteins from the solid state under cold vacuum conditions is described. This matrix assisted ionization vacuum (MAIV) mass spectrometry (MS) method produces multiply charged ions similar to those that typify electrospray ionization (ESI) and uses sample preparation methods that are nearly identical to matrix-assisted laser desorption/ionization (MALDI). Unlike these established methods, MAIV does not require a laser or voltage for ionization, and unlike the recently introduced matrix assisted ionization inlet method, does not require added heat. MAIV-MS requires only introduction of a crystalline mixture of the analyte incorporated with a suitable small molecule matrix compound such as 3-nitrobenzonitrile directly to the vacuum of the mass spectrometer. Vacuum intermediate pressure MALDI sources and modified ESI sources successfully produce ions for analysis by MS with this method. As in ESI-MS, ion formation is continuous and, without a laser, little chemical background is observed. MAIV, operating from a surface offers the possibility of significantly improved sensitivity relative to atmospheric pressure ionization because ions are produced in the vacuum region of the mass spectrometer eliminating losses associated with ion transfer from atmospheric pressure to vacuum. Mechanistic aspects and potential applications for this new ionization method are discussed.
MeSH term(s)	Animals ; Gases/chemistry ; Horses ; Pharmaceutical Preparations/analysis ; Pharmaceutical Preparations/chemistry ; Proteins/analysis ; Proteins/chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Vacuum
Chemical Substances	Gases ; Pharmaceutical Preparations ; Proteins
Language	English
Publishing date	2013-03-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1073671-2
ISSN	1879-1123 ; 1044-0305
ISSN (online)	1879-1123
ISSN	1044-0305
DOI	10.1007/s13361-012-0571-z
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Article ; Online: New ionization method for analysis on atmospheric pressure ionization mass spectrometers requiring only vacuum and matrix assistance.

Trimpin, Sarah / Inutan, Ellen D

Analytical chemistry

2013 Volume 85, Issue 4, Page(s) 2005–2009

Abstract: Matrix assisted ionization vacuum (MAIV) is a new ionization method that does not require high voltages, a laser beam, or applied heat and depends only the proper matrix, 3-nitrobenzonitrile (3-NBN), and the vacuum of the mass spectrometer to initiate ... ...

Abstract	Matrix assisted ionization vacuum (MAIV) is a new ionization method that does not require high voltages, a laser beam, or applied heat and depends only the proper matrix, 3-nitrobenzonitrile (3-NBN), and the vacuum of the mass spectrometer to initiate ionization. Analyte ions of volatile as well as nonvolatile compounds are formed by simply exposing the matrix-analyte to the vacuum of a mass spectrometer. The reduced pressure at the inlet of an atmospheric pressure ionization mass spectrometer suffices to produce analyte ions, but unlike the previously reported matrix assisted ionization inlet method, with MAIV, heating the inlet is not necessary. Singly and multiply charged ions are formed similar to electrospray ionization but from a surface. Mass spectrometers in which a heated inlet tube is not available can be used for ionization using the 3-NBN matrix. We demonstrate rapid, high-sensitivity analyses of drugs, peptides, and proteins in the low femtomole range. The potential for high-throughput analyses is shown using multiwell plates and paper strips.
Language	English
Publishing date	2013-02-19
Publishing country	United States
Document type	Letter
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/ac303717j
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Article: New Ionization Method for Analysis on Atmospheric Pressure Ionization Mass Spectrometers Requiring Only Vacuum and Matrix Assistance

Trimpin, Sarah / Inutan Ellen D

Analytical chemistry. 2013 Feb. 19, v. 85, no. 4

2013

Abstract	Matrix assisted ionization vacuum (MAIV) is a new ionization method that does not require high voltages, a laser beam, or applied heat and depends only the proper matrix, 3-nitrobenzonitrile (3-NBN), and the vacuum of the mass spectrometer to initiate ionization. Analyte ions of volatile as well as nonvolatile compounds are formed by simply exposing the matrixâanalyte to the vacuum of a mass spectrometer. The reduced pressure at the inlet of an atmospheric pressure ionization mass spectrometer suffices to produce analyte ions, but unlike the previously reported matrix assisted ionization inlet method, with MAIV, heating the inlet is not necessary. Singly and multiply charged ions are formed similar to electrospray ionization but from a surface. Mass spectrometers in which a heated inlet tube is not available can be used for ionization using the 3-NBN matrix. We demonstrate rapid, high-sensitivity analyses of drugs, peptides, and proteins in the low femtomole range. The potential for high-throughput analyses is shown using multiwell plates and paper strips.
Keywords	atmospheric pressure ; drugs ; electric power ; heat ; ionization ; ions ; paper ; peptides ; proteins ; spectrometers
Language	English
Dates of publication	2013-0219
Size	p. 2005-2009.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021%2Fac303717j
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Matrix assisted ionization vacuum (MAIV), a new ionization method for biological materials analysis using mass spectrometry.

Inutan, Ellen D / Trimpin, Sarah

Molecular & cellular proteomics : MCP

2012 Volume 12, Issue 3, Page(s) 792–796

Abstract: The introduction of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) for the mass spectrometric analysis of peptides and proteins had a dramatic impact on biological science. We now report that a wide variety of ... ...

Abstract	The introduction of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) for the mass spectrometric analysis of peptides and proteins had a dramatic impact on biological science. We now report that a wide variety of compounds, including peptides, proteins, and protein complexes, are transported directly from a solid-state small molecule matrix to gas-phase ions when placed into the vacuum of a mass spectrometer without the use of high voltage, a laser, or added heat. This ionization process produces ions having charge states similar to ESI, making the method applicable for high performance mass spectrometers designed for atmospheric pressure ionization. We demonstrate highly sensitive ionization using intermediate pressure MALDI and modified ESI sources. This matrix and vacuum assisted soft ionization method is suitable for the direct surface analysis of biological materials, including tissue, via mass spectrometry.
MeSH term(s)	Gases/analysis ; Gases/chemistry ; Ions/analysis ; Ions/chemistry ; Nitriles/chemistry ; Nitrobenzenes/chemistry ; Peptides/analysis ; Peptides/chemistry ; Proteins/analysis ; Proteins/chemistry ; Reproducibility of Results ; Spectrometry, Mass, Electrospray Ionization/methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Vacuum
Chemical Substances	Gases ; Ions ; Nitriles ; Nitrobenzenes ; Peptides ; Proteins ; 3-nitrobenzonitrile (619-24-9)
Language	English
Publishing date	2012-12-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2075924-1
ISSN	1535-9484 ; 1535-9476
ISSN (online)	1535-9484
ISSN	1535-9476
DOI	10.1074/mcp.M112.023663
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Zs.A 5599: Show issues

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Article ; Online: Expression and

Muthunayake, Nisansala S / Islam, Rabiul / Inutan, Ellen D / Colangelo, Wesley / Trimpin, Sarah / Cunningham, Philip R / Chow, Christine S

Biochemistry

2020 Volume 59, Issue 36, Page(s) 3380–3391

Abstract: Peptides have important biomedical applications, but poor correlation ... ...

Abstract	Peptides have important biomedical applications, but poor correlation between
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Antimicrobial Cationic Peptides/chemistry ; Antimicrobial Cationic Peptides/genetics ; Antimicrobial Cationic Peptides/metabolism ; Base Sequence ; Escherichia coli/drug effects ; Escherichia coli/growth & development ; Escherichia coli/metabolism ; Microbial Sensitivity Tests ; Mutation ; Pore Forming Cytotoxic Proteins/pharmacology ; Ribosomes/chemistry ; Ribosomes/metabolism ; Sequence Homology
Chemical Substances	Anti-Bacterial Agents ; Antimicrobial Cationic Peptides ; Pore Forming Cytotoxic Proteins ; oncocin
Language	English
Publishing date	2020-09-01
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1108-3
ISSN	1520-4995 ; 0006-2960
ISSN (online)	1520-4995
ISSN	0006-2960
DOI	10.1021/acs.biochem.0c00600
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Article ; Online: Sublimation Driven Ionization for Use in Mass Spectrometry: Mechanistic Implications.

McEwen, Charles N / Inutan, Ellen D / Moreno-Pedraza, Abigail / Lu, I-Chung / Hoang, Khoa / Pophristic, Milan / Trimpin, Sarah

Journal of the American Society for Mass Spectrometry

2020 Volume 32, Issue 1, Page(s) 114–123

Abstract: Sublimation has been known at least since the middle ages. This process is frequently taught in schools through the use of phase diagrams. Astonishingly, such a well-known process appears to still harbor secrets. Under conditions in which compound ... ...

Abstract	Sublimation has been known at least since the middle ages. This process is frequently taught in schools through the use of phase diagrams. Astonishingly, such a well-known process appears to still harbor secrets. Under conditions in which compound sublimation occurs, gas-phase ions are frequently detected using mass spectrometry. This was exploited in matrix-assisted ionization in vacuum (vMAI) by adding analyte to subliming compounds used as matrices. Good vMAI matrices were those that ionize the added analyte with high sensitivity, but even matrices that fail this test often produce ions of likely matrix impurities suggesting that they may be good matrices for some compound types. We also show that binary matrices may be manipulated to provide desired properties such as fast analyses and improved sensitivity. These results imply that sublimation in some cases is more complicated than just molecules leaving a surface and that understanding the physical force responsible, and how the nonvolatile compound becomes charged, could lead to improved ionization efficiency for mass spectrometry. Here we provide insights into this process and an explanation of why this unexpected phenomenon has not previously been reported.
Language	English
Publishing date	2020-12-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	1073671-2
ISSN	1879-1123 ; 1044-0305
ISSN (online)	1879-1123
ISSN	1044-0305
DOI	10.1021/jasms.0c00297
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Article ; Online: Laserspray ionization (LSI) ion mobility spectrometry (IMS) mass spectrometry.

Inutan, Ellen / Trimpin, Sarah

Journal of the American Society for Mass Spectrometry

2010 Volume 21, Issue 7, Page(s) 1260–1264

Abstract: A simple device is described for desolvation of highly charged matrix/analyte clusters produced by laser ablation leading to multiply charged ions that are analyzed by ion mobility spectrometry-mass spectrometry. Thus, for example, highly charged ions of ...

Abstract	A simple device is described for desolvation of highly charged matrix/analyte clusters produced by laser ablation leading to multiply charged ions that are analyzed by ion mobility spectrometry-mass spectrometry. Thus, for example, highly charged ions of ubiquitin and lysozyme are cleanly separated in the gas phase according to size and mass (shape and molecular weight) as well as charge using Tri-Wave ion mobility technology coupled to mass spectrometry. This contribution confirms the mechanistic argument that desolvation is necessary to produce multiply charged matrix-assisted laser desorption/ionization (MALDI) ions and points to how these ions can be routinely formed on any atmospheric pressure mass spectrometer.
Language	English
Publishing date	2010-04-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	1073671-2
ISSN	1879-1123 ; 1044-0305
ISSN (online)	1879-1123
ISSN	1044-0305
DOI	10.1016/j.jasms.2010.03.039
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Article ; Online: Laserspray ionization-ion mobility spectrometry-mass spectrometry: baseline separation of isomeric amyloids without the use of solvents desorbed and Ionized directly from a surface.

Inutan, Ellen D / Trimpin, Sarah

Journal of proteome research

2010 Volume 9, Issue 11, Page(s) 6077–6081

Abstract: The ability of laserspray ionization (LSI) to produce multiply charged ions by laser ablation from the solid state, directly from a surface, and at atmospheric pressure allows protein analysis on an ion mobility spectrometry (IMS)-mass spectrometry (MS) ... ...

Abstract	The ability of laserspray ionization (LSI) to produce multiply charged ions by laser ablation from the solid state, directly from a surface, and at atmospheric pressure allows protein analysis on an ion mobility spectrometry (IMS)-mass spectrometry (MS) instrument (SYNAPT G2) having a mass-to-charge limit of 8000. The matrix, 2,5-dihydroxyacetophenone, lowers the thermal requirements for desolvation of matrix/analyte clusters to produce the highly charged LSI ions under gentle conditions to retain structural integrity of the proteins. Examples include cytochrome C and lysozyme. The solvent-free IMS gas-phase separation is used to baseline separate in the drift time dimension the isomeric solubility restricted β-amyloid (1-42) from the reversed (42-1). The LSI process is shown to be sufficiently soft to preserve structural integrity and permit separation according to the different shapes. These results suggest that LSI-IMS-MS potentially combines speed of analysis and imaging capability common to matrix-assisted laser desorption/ionization and multiple charging with the potential for structural analysis common to electrospray ionization.
MeSH term(s)	Amyloid/analysis ; Amyloid/chemistry ; Amyloid beta-Peptides/analysis ; Amyloid beta-Peptides/chemistry ; Amyloid beta-Peptides/classification ; Humans ; Isomerism ; Peptide Fragments/analysis ; Peptide Fragments/chemistry ; Peptide Fragments/classification ; Protein Conformation ; Spectrometry, Mass, Electrospray Ionization/methods
Chemical Substances	Amyloid ; Amyloid beta-Peptides ; Peptide Fragments ; amyloid beta-protein (1-42)
Language	English
Publishing date	2010-11-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/pr1005923
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