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Article ; Online: Towards a Canary Islands barcode database for soil biodiversity

Irene Santos-Perdomo / Daniel Suárez / María L. Moraza / Paula Arribas / Carmelo Andújar

Biodiversity Data Journal, Vol 12, Iss , Pp 1-

revealing cryptic and unrecorded mite species diversity within insular soils

2024 Volume 41

Abstract: Soil arthropod diversity contributes to a high proportion of the total biodiversity on Earth. However, most soil arthropods are still undescribed, hindering our understanding of soil functioning and global biodiversity estimations. Inventorying soil ... ...

Abstract	Soil arthropod diversity contributes to a high proportion of the total biodiversity on Earth. However, most soil arthropods are still undescribed, hindering our understanding of soil functioning and global biodiversity estimations. Inventorying soil arthropods using conventional taxonomical approaches is particularly difficult and costly due to the great species richness, abundance and local-scale heterogeneity of mesofauna communities and the poor taxonomic background knowledge of most lineages. To alleviate this situation, we have designed and implemented a molecular barcoding framework adapted to soil fauna. This pipeline includes different steps, starting with a morphology-based selection of specimens which are imaged. Then, DNA is extracted non-destructively. Both images and voucher specimens are used to assign a taxonomic identification, based on morphology that is further checked for consistency with molecular information. Using this procedure, we studied 239 specimens of mites from the Canary Islands including representatives of Mesostigmata, Sarcoptiformes and Trombidiformes, of which we recovered barcode sequences for 168 specimens that were morphologically identified to 49 species, with nine specimens that could only be identified at the genus or family levels. Multiple species delimitation analyses were run to compare molecular delimitations with morphological identifications, including ASAP, mlPTP, BINs and 3% and 8% genetic distance thresholds. Additionally, a species-level search was carried out at the Biodiversity Databank of the Canary Islands (BIOTA) to evaluate the number of species in our dataset that were not previously recorded in the archipelago. In parallel, a sequence-level search of our sequences was performed against BOLD Systems. Our results reveal that multiple morphologically identified species correspond to different molecular lineages, which points to significant levels of unknown cryptic diversity within the archipelago. In addition, we evidenced that multiple species in our ...
Keywords	Acari ; COI ; barcoding ; soil mesofauna ; biotic fron ; Biology (General) ; QH301-705.5
Subject code	590
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Pensoft Publishers
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Off-the-shelf proximity biotinylation for interaction proteomics

Irene Santos-Barriopedro / Guido van Mierlo / Michiel Vermeulen

Nature Communications, Vol 12, Iss 1, Pp 1-

2021 Volume 12

Abstract: Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling ... ...

Abstract	Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling interactome profiling of endogenous proteins or PTMs.
Keywords	Science ; Q
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Cuidadores formais e suas necessidades de educação para a saúde

Helena Falcão / Irene Santos / Isabel Fonseca / Rui Coelho

Cadernos de Saúde, Vol 12, Iss Especial (2021)

conhecer no presente para atuar no futuro

2021

Keywords	Medicine (General) ; R5-920 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	Universidade Católica Portuguesa, Instituto de Ciências da Saúde
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Proceso de análisis del dominio ágil de sistemas integrados de salud en el contexto venezolano

Francisca Losavio / Oscar Ordaz / Irene Santos

Enl@ce, Vol 12, Iss 1, Pp 101-

2015 Volume 134

Abstract: El diseño de arquitecturas de sistemas integrados (SI) es un reto de la Ingeniería del Software, más aún en un contexto de producción industrial. Este trabajo, propone un proceso de análisis del dominio ágil (ADA) de Sistemas de Información Integrados de ...

Abstract	El diseño de arquitecturas de sistemas integrados (SI) es un reto de la Ingeniería del Software, más aún en un contexto de producción industrial. Este trabajo, propone un proceso de análisis del dominio ágil (ADA) de Sistemas de Información Integrados de Salud (SIS) considerando reglas del negocio, para modelar requisitos no funcionales (RNF) globales y relativos a estilos arquitectónicos, y la identificación de requisitos funcionales (RF), puede ser utilizado como entrada a procesos ascendentes o “bottom-up” para construir la arquitectura de referencia (AR), donde no se contemplan etapas de ADA porque se centran en refactorización de productos existentes. En ADA, los RNF son especificados mediante el modelo de calidad estándar ISO/IEC 25010, que representa la calidad del producto de software y facilita así la comunicación, se estudian componentes de productos existentes y no el modelado global de características, como ocurre en enfoques clásicos descendentes o “top-down”. Para el desarrollo del estudio se considera el contexto venezolano, donde se analizan proyectos en Venezuela y un estudio de campo sobre una muestra de institutos de salud del país, para obtener un panorama de la situación actual. Se aprecia la necesidad de la pronta implantación de SIS con historias clínicas electrónicas interoperables y servicios generales de telemedicina. ADA, puede ser reutilizado en otros dominios y es usado para “validar” una AR-SIS, obtenida por un proceso ascendente definido en trabajos anteriores, el cual solo consideraba el estudio de productos existentes
Keywords	ingeniería del software ; requisitos no funcionales ; sistema de información integrado de salud ; telemedicina ; ISO/IEC 25010 ; Bibliography. Library science. Information resources ; Z
Language	Spanish
Publishing date	2015-04-01T00:00:00Z
Publisher	Universidad del Zulia
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A database of functional traits for spiders from native forests of the Iberian Peninsula and Macaronesia

Nuria Macías-Hernández / Cândida Ramos / Marc Domènech / Sara Febles / Irene Santos / Miquel Arnedo / Paulo Borges / Brent Emerson / Pedro Cardoso

Biodiversity Data Journal, Vol 8, Iss , Pp 1-

2020 Volume 12

Keywords	Araneae ; ecology ; forest ; morphology ; Portugal ; Sp ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2020-04-01T00:00:00Z
Publisher	Pensoft Publishers
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: (–)-Epigallocatechin-3-gallate induces apoptosis and differentiation in leukaemia by targeting reactive oxygen species and PIN1

Fernanda Isabel Della Via / Rodrigo Naoto Shiraishi / Irene Santos / Karla Priscila Ferro / Myriam Janeth Salazar-Terreros / Gilberto Carlos Franchi Junior / Eduardo Magalhães Rego / Sara Teresinha Olalla Saad / Cristiane Okuda Torello

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 11

Abstract: Abstract (–)-Epigallocatechin-3-gallate (EGCG), the major active polyphenol extracted from green tea, has been shown to induce apoptosis and inhibit cell proliferation, cell invasion, angiogenesis and metastasis. Herein, we evaluated the in vivo effects ... ...

Abstract	Abstract (–)-Epigallocatechin-3-gallate (EGCG), the major active polyphenol extracted from green tea, has been shown to induce apoptosis and inhibit cell proliferation, cell invasion, angiogenesis and metastasis. Herein, we evaluated the in vivo effects of EGCG in acute myeloid leukaemia (AML) using an acute promyelocytic leukaemia (APL) experimental model (PML/RARα). Haematological analysis revealed that EGCG treatment reversed leucocytosis, anaemia and thrombocytopenia, and prolonged survival of PML/RARα mice. Notably, EGCG reduced leukaemia immature cells and promyelocytes in the bone marrow while increasing mature myeloid cells, possibly due to apoptosis increase and cell differentiation. The reduction of promyelocytes and neutrophils/monocytes increase detected in the peripheral blood, in addition to the increased percentage of bone marrow cells with aggregated promyelocytic leukaemia (PML) bodies staining and decreased expression of PML-RAR oncoprotein corroborates our results. In addition, EGCG increased expression of neutrophil differentiation markers such as CD11b, CD14, CD15 and CD66 in NB4 cells; and the combination of all-trans retinoic acid (ATRA) plus EGCG yield higher increase the expression of CD15 marker. These findings could be explained by a decrease of peptidyl-prolyl isomerase NIMA-interacting 1 (PIN1) expression and reactive oxygen species (ROS) increase. EGCG also decreased expression of substrate oncoproteins for PIN1 (including cyclin D1, NF-κB p65, c-MYC, and AKT) and 67 kDa laminin receptor (67LR) in the bone marrow cells. Moreover, EGCG showed inhibition of ROS production in NB4 cells in the presence of N-acetyl-L-cysteine (NAC), as well as a partial blockage of neutrophil differentiation and apoptosis, indicating that EGCG-activities involve/or are in response of oxidative stress. Furthermore, apoptosis of spleen cells was supported by increasing expression of BAD and BAX, parallel to BCL-2 and c-MYC decrease. The reduction of spleen weights of PML/RARα mice, as well as apoptosis induced by EGCG in NB4 cells in a dose-dependent manner confirms this assumption. Our results support further evaluation of EGCG in clinical trials for AML, since EGCG could represent a promising option for AML patient ineligible for current mainstay treatments.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Arhgap21 Deficiency Results in Increase of Osteoblastic Lineage Cells in the Murine Bone Marrow Microenvironment

Mariana Ferreira Pissarra / Cristiane Okuda Torello / Rafael Gonçalves Barbosa Gomes / Rodrigo Naoto Shiraishi / Irene Santos / Karla Priscila Vieira Ferro / Matheus Rodrigues Lopes / Patricia Maria Bergamo Favaro / Sara Teresinha Olalla Saad / Mariana Lazarini

Frontiers in Cell and Developmental Biology, Vol

2021 Volume 9

Abstract: ARHGAP21 is a member of the RhoGAP family of proteins involved in cell growth, differentiation, and adhesion. We have previously shown that the heterozygous Arhgap21 knockout mouse model (Arhgap21+/−) presents several alterations in the hematopoietic ... ...

Abstract	ARHGAP21 is a member of the RhoGAP family of proteins involved in cell growth, differentiation, and adhesion. We have previously shown that the heterozygous Arhgap21 knockout mouse model (Arhgap21+/−) presents several alterations in the hematopoietic compartment, including increased frequency of hematopoietic stem and progenitor cells (HSPC) with impaired adhesion in vitro, increased mobilization to peripheral blood, and decreased engraftment after bone marrow transplantation. Although these HSPC functions strongly depend on their interactions with the components of the bone marrow (BM) niche, the role of ARHGAP21 in the marrow microenvironment has not yet been explored. In this study, we investigated the composition and function of the BM microenvironment in Arhgap21+/− mice. The BM of Arhgap21+/− mice presented a significant increase in the frequency of phenotypic osteoblastic lineage cells, with no differences in the frequencies of multipotent stromal cells or endothelial cells when compared to the BM of wild type mice. Arhgap21+/− BM cells had increased capacity of generating osteogenic colony-forming units (CFU-OB) in vitro and higher levels of osteocalcin were detected in the Arhgap21+/− BM supernatant. Increased expression of Col1a1, Ocn and decreased expression of Trap1 were observed after osteogenic differentiation of Arhgap21+/− BM cells. In addition, Arhgap21+/− mice recipients of normal BM cells showed decreased leucocyte numbers during transplantation recovery. Our data suggest participation of ARHGAP21 in the balanced composition of the BM microenvironment through the regulation of osteogenic differentiation.
Keywords	osteocalcin ; myelodysplastic syndromes ; acute myeloid leukemia ; RhoGAP ; Rho GTPase ; RhoA ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2021-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
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Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Retinal Ganglion Cell Loss and Microglial Activation in a SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis

Pilar Rojas / Ana I. Ramírez / Manuel Cadena / José A. Fernández-Albarral / Elena Salobrar-García / Inés López-Cuenca / Irene Santos-García / Eva de Lago / José L. Urcelay-Segura / José M. Ramírez / Rosa de Hoz / Juan J. Salazar

International Journal of Molecular Sciences, Vol 22, Iss 4, p

2021 Volume 1663

Abstract: The neurodegenerative disease amyotrophic lateral sclerosis (ALS) affects the spinal cord, brain stem, and cerebral cortex. In this pathology, both neurons and glial cells are affected. However, few studies have analyzed retinal microglia in ALS models. ... ...

Abstract	The neurodegenerative disease amyotrophic lateral sclerosis (ALS) affects the spinal cord, brain stem, and cerebral cortex. In this pathology, both neurons and glial cells are affected. However, few studies have analyzed retinal microglia in ALS models. In this study, we quantified the signs of microglial activation and the number of retinal ganglion cells (RGCs) in an SOD1G93A transgenic mouse model at 120 days (advanced stage of the disease) in retinal whole-mounts. For SOD1G93A animals (compared to the wild-type), we found, in microglial cells, (i) a significant increase in the area occupied by each microglial cell in the total area of the retina; (ii) a significant increase in the arbor area in the outer plexiform layer (OPL) inferior sector; (iii) the presence of cells with retracted processes; (iv) areas of cell groupings in some sectors; (v) no significant increase in the number of microglial cells; (vi) the expression of IFN-γ and IL-1β; and (vii) the non-expression of IL-10 and arginase-I. For the RGCs, we found a decrease in their number. In conclusion, in the SOD1G93A model (at 120 days), retinal microglial activation occurred, taking a pro-inflammatory phenotype M1, which affected the OPL and inner retinal layers and could be related to RGC loss.
Keywords	microglia ; retina ; SOD1G93A mouse model ; ALS ; retinal whole-mount ; microglial activation ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	610
Language	English
Publishing date	2021-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Comparison of different methods to overexpress large genes

Aline Lisie Ramos / Fernanda Soares Niemann / Adriana Silva Santos Duarte / Karla Priscila Ferro / Irene Santos / Carolina Louzão Bigarella / Antonio Filareto / Sara Teresinha Olalla Saad

Journal of Biological Research, Vol 91, Iss

2018 Volume 2

Abstract: Gain-of-function of very large transgene constructs can lead to genetic perturbations, providing researchers with the alternative of a powerful tool to identify pathway components which remain undetected when using traditional loss-of-function analysis. ... ...

Abstract	Gain-of-function of very large transgene constructs can lead to genetic perturbations, providing researchers with the alternative of a powerful tool to identify pathway components which remain undetected when using traditional loss-of-function analysis. To promote longer-term expression, various systems for transgene integration have been developed, however large cDNA sequences are often difficult to clone into size-limited expression vectors. We attempted to overexpress ARHGAP21, a 5.874 kb gene, using different methodologies as plasmid, lentiviral and Sleeping Beauty (SB) transposon based gene transfer. Using lentiviral based transduction; an enormous amount of lentiviral supernatant was produced to obtain a satisfactory titration after double ultracentrifugation. However, U937 transduced cells showed only 50% of gene expression increase, which vanished after 5 days. SB transposon system application to overexpress ARHGAP21 was a complete success. Nucleofecting SB-based vector plus SB100x transposase vector resulted in an expressive increase of gene and protein expression. Furthermore, the overexpression was maintained even after freezing and thawing processes. In conclusion, our work shows that the SB transposon system is the best choice for those seeking a stable and high gene expression. Once the overexpression is achieved, freezing cells and using them for a long time becomes possible.
Keywords	Gene Overexpression ; Large Genes ; Transgene Integration ; Transposon System ; Lentiviral System ; DNA Transfer Systems ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2018-10-01T00:00:00Z
Publisher	PAGEPress Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: SIRT6-dependent cysteine monoubiquitination in the PRE-SET domain of Suv39h1 regulates the NF-κB pathway

Irene Santos-Barriopedro / Laia Bosch-Presegué / Anna Marazuela-Duque / Carolina de la Torre / Carlota Colomer / Berta N. Vazquez / Thomas Fuhrmann / Bárbara Martínez-Pastor / Wenfu Lu / Thomas Braun / Eva Bober / Thomas Jenuwein / Lourdes Serrano / Manel Esteller / Zhenbang Chen / Silvia Barceló-Batllori / Raúl Mostoslavsky / Lluis Espinosa / Alejandro Vaquero

Nature Communications, Vol 9, Iss 1, Pp 1-

2018 Volume 15

Abstract: Sirtuins are involved in the regulation of responses to diverse types of cellular stress. Here the authors describe the SirT6-dependent cysteine monoubiquitination of the histone methyltransferase Suv39h1 as part of a regulatory circuit for the NF-κB ... ...

Abstract	Sirtuins are involved in the regulation of responses to diverse types of cellular stress. Here the authors describe the SirT6-dependent cysteine monoubiquitination of the histone methyltransferase Suv39h1 as part of a regulatory circuit for the NF-κB pathway.
Keywords	Science ; Q
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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