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  1. Article ; Online: Monitoring non-pharmaceutical public health interventions during the COVID-19 pandemic

    Yannan Shen / Guido Powell / Iris Ganser / Qulu Zheng / Chris Grundy / Anya Okhmatovskaia / David L. Buckeridge

    Scientific Data, Vol 8, Iss 1, Pp 1-

    2021  Volume 6

    Abstract: Measuring and monitoring non-pharmaceutical interventions is important yet challenging due to the need to clearly define and encode non-pharmaceutical interventions, to collect geographically and socially representative data, and to accurately document ... ...

    Abstract Measuring and monitoring non-pharmaceutical interventions is important yet challenging due to the need to clearly define and encode non-pharmaceutical interventions, to collect geographically and socially representative data, and to accurately document the timing at which interventions are initiated and changed. These challenges highlight the importance of integrating and triangulating across multiple databases and the need to expand and fund the mandate for public health organizations to track interventions systematically.
    Keywords Science ; Q
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Targeted transcript quantification in single disseminated cancer cells after whole transcriptome amplification.

    Franziska C Durst / Ana Grujovic / Iris Ganser / Martin Hoffmann / Peter Ugocsai / Christoph A Klein / Zbigniew T Czyż

    PLoS ONE, Vol 14, Iss 8, p e

    2019  Volume 0216442

    Abstract: Gene expression analysis of rare or heterogeneous cell populations such as disseminated cancer cells (DCCs) requires a sensitive method allowing reliable analysis of single cells. Therefore, we developed and explored the feasibility of a quantitative PCR ...

    Abstract Gene expression analysis of rare or heterogeneous cell populations such as disseminated cancer cells (DCCs) requires a sensitive method allowing reliable analysis of single cells. Therefore, we developed and explored the feasibility of a quantitative PCR (qPCR) assay to analyze single-cell cDNA pre-amplified using a previously established whole transcriptome amplification (WTA) protocol. We carefully selected and optimized multiple steps of the protocol, e.g. re-amplification of WTA products, quantification of amplified cDNA yields and final qPCR quantification, to identify the most reliable and accurate workflow for quantitation of gene expression of the ERBB2 gene in DCCs. We found that absolute quantification outperforms relative quantification. We then validated the performance of our method on single cells of established breast cancer cell lines displaying distinct levels of HER2 protein. The different protein levels were faithfully reflected by transcript expression across the tested cell lines thereby proving the accuracy of our approach. Finally, we applied our method to breast cancer DCCs of a patient undergoing anti-HER2-directed therapy. Here, we were able to measure ERBB2 expression levels in all HER2-protein-positive DCCs. In summary, we developed a reliable single-cell qPCR assay applicable to measure distinct levels of ERBB2 in DCCs.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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