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  1. Article ; Online: Optimizing chili production in drought stress: combining Zn-quantum dot biochar and proline for improved growth and yield.

    Hareem, Misbah / Danish, Subhan / Pervez, Mahnoor / Irshad, Usman / Fahad, Shah / Dawar, Khadim / Alharbi, Sulaiman Ali / Ansari, Mohammad Javed / Datta, Rahul

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 6627

    Abstract: The reduction in crop productivity due to drought stress, is a major concern in agriculture. Drought stress usually disrupts photosynthesis by triggering oxidative stress and generating reactive oxygen species (ROS). The use of zinc-quantum dot biochar ( ... ...

    Abstract The reduction in crop productivity due to drought stress, is a major concern in agriculture. Drought stress usually disrupts photosynthesis by triggering oxidative stress and generating reactive oxygen species (ROS). The use of zinc-quantum dot biochar (ZQDB) and proline (Pro) can be effective techniques to overcome this issue. Biochar has the potential to improve the water use efficiency while proline can play an imperative role in minimization of adverse impacts of ROS Proline, functioning as an osmotic protector, efficiently mitigates the adverse effects of heavy metals on plants by maintaining cellular structure, scavenging free radicals, and ensuring the stability of cellular integrity. That's why current study explored the impact of ZQDB and proline on chili growth under drought stress. Four treatments, i.e., control, 0.4%ZQDB, 0.1 mM Pro, and 0.4%ZQDB + Pro, were applied in 4 replications following the complete randomized design. Results exhibited that 0.4%ZQDB + Pro caused an increases in chili plant dry weight (29.28%), plant height (28.12%), fruit length (29.20%), fruit girth (59.81%), and fruit yield (55.78%) over control under drought stress. A significant increment in chlorophyll a (18.97%), chlorophyll b (49.02%), and total chlorophyll (26.67%), compared to control under drought stress, confirmed the effectiveness of 0.4%ZQDB + Pro. Furthermore, improvement in leaves N, P, and K concentration over control validated the efficacy of 0.4%ZQDB + Pro against drought stress. In conclusion, 0.4%ZQDB + Pro can mitigate drought stress in chili. More investigations are suggested to declare 0.4%ZQDB + Pro as promising amendment for mitigation of drought stress in other crops as well under changing climatic situations.
    MeSH term(s) Chlorophyll A ; Reactive Oxygen Species ; Droughts ; Proline ; Quantum Dots ; Zinc ; Charcoal
    Chemical Substances Chlorophyll A (YF5Q9EJC8Y) ; biochar ; Reactive Oxygen Species ; Proline (9DLQ4CIU6V) ; Zinc (J41CSQ7QDS) ; Charcoal (16291-96-6)
    Language English
    Publishing date 2024-03-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-57204-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Author Correction: Optimizing chili production in drought stress: combining Zn-quantum dot biochar and proline for improved growth and yield.

    Hareem, Misbah / Danish, Subhan / Pervez, Mahnoor / Irshad, Usman / Fahad, Shah / Dawar, Khadim / Alharbi, Sulaiman Ali / Ansari, Mohammad Javed / Datta, Rahul

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 8333

    Language English
    Publishing date 2024-04-09
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-59029-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Ectomycorrhiza and ethylenediurea reduced the impact of high nitrogen and ozone stresses and increased the growth of

    Jamil, Hafiz Muhammad Ansab / Gatasheh, Mansour K / Ahmad, Rafiq / Ibrahim, Khalid Elfaki / Khan, Sabaz Ali / Irshad, Usman / Shahzad, Muhammad / Abbasi, Arshad Mehmood

    Heliyon

    2024  Volume 10, Issue 7, Page(s) e28635

    Abstract: ... Cedrus ... ...

    Abstract Cedrus deodara
    Language English
    Publishing date 2024-03-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e28635
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Bacterial Subspecies Variation and Nematode Grazing Change P Dynamics in the Wheat Rhizosphere.

    Irshad, Usman / Yergeau, Etienne

    Frontiers in microbiology

    2018  Volume 9, Page(s) 1990

    Abstract: Low phosphorus soils are thought to constitute the majority of soils worldwide and cannot support intensive agriculture without high fertilizer inputs. Rhizobacteria are well-known to modify P dynamics and an increased bacterial diversity normally has a ... ...

    Abstract Low phosphorus soils are thought to constitute the majority of soils worldwide and cannot support intensive agriculture without high fertilizer inputs. Rhizobacteria are well-known to modify P dynamics and an increased bacterial diversity normally has a positive impact on various process rates. However, it is not known how variation in bacterial diversity at the subspecies level could influence trophic interactions in the rhizosphere and its consequences on plant P nutrition. We therefore hypothesized that the interactions between closely related P solubilizing bacteria and their grazing nematodes could improve plant P dynamics from an unavailable P source. We isolated four
    Language English
    Publishing date 2018-09-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2018.01990
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Organic amendment plus inoculum drivers: Who drives more P nutrition for wheat plant fitness in small duration soil experiment.

    Ahmed, Saba / Iqbal, Nadeem / Tang, Xiaoyan / Ahmad, Rafiq / Irshad, Muhammad / Irshad, Usman

    PloS one

    2022  Volume 17, Issue 4, Page(s) e0266279

    Abstract: Functioning of ecosystems depends on the nutrient dynamics across trophic levels, largely mediated by microbial interactions in the soil food web. The present study investigated the use of phosphate solubilizing bacteria (PSB) and poultry manure (PM) for ...

    Abstract Functioning of ecosystems depends on the nutrient dynamics across trophic levels, largely mediated by microbial interactions in the soil food web. The present study investigated the use of phosphate solubilizing bacteria (PSB) and poultry manure (PM) for maintaining labile P in the soil for an extensive fertility enhancement and as a substitution of chemical fertilizers. Based on the different P solubilizing capabilities of Bacillus and Pseudomonas, a quadruple consortium of Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis and Pseudomonas fluorescens, and their grazer nematodes (soil free living) supplemented with PM were studied. This study was carried out on the trophic levels of soil communities to assess the growth and availability of P to the wheat plants. Experiment was performed for 90 days. Comparing the unamended and amended predator results showed that nematode addition beyond bacterial treatment substantially increased the net available P by ≈2 times, and alkaline phosphatase (ALP) activity by 3.3 times. These results demonstrated the nematodes association with increasing nutrient availability or P mineralization. The interactive effect of PM as substrate and biological drivers was more noticeable on plant dry biomass (1.6 times) and plant P concentration (3.5times) compared to the similar unamended treatment. It is concluded that the biological drivers significantly enhanced the soil ALP and available P while the substrate and biological drivers enhanced dry biomass and plant P concentration. Bacterivore nematodes enhanced the effect of PSB for P mineralization via microbial loop and could be used for the enhancement of wheat production.
    MeSH term(s) Ecosystem ; Phosphates ; Pseudomonas fluorescens ; Soil ; Soil Microbiology ; Triticum
    Chemical Substances Phosphates ; Soil
    Language English
    Publishing date 2022-04-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0266279
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Richness of Rhizosphere Organisms Affects Plant P Nutrition According to P Source and Mobility

    Trap, Jean / Ranoarisoa, Patricia Mahafaka / Irshad, Usman / Plassard, Claude

    Agriculture (Basel). 2021 Feb. 16, v. 11, no. 2

    2021  

    Abstract: Plants evolve complex interactions with diverse soil mutualist organisms to enhance P mobilization from the soil. These strategies are particularly important when P is poorly available. It is still unclear how the soil P source (e.g., mineral P versus ... ...

    Abstract Plants evolve complex interactions with diverse soil mutualist organisms to enhance P mobilization from the soil. These strategies are particularly important when P is poorly available. It is still unclear how the soil P source (e.g., mineral P versus recalcitrant organic P) and its mobility in the soil (high or low) affect soil mutualist biological (ectomycorrhizal fungi, bacteria and bacterial-feeding nematodes) richness—plant P acquisition relationships. Using a set of six microcosm experiments conducted in growth chamber across contrasting P situations, we tested the hypothesis that the relationship between the increasing addition of soil mutualist organisms in the rhizosphere of the plant and plant P acquisition depends on P source and mobility. The highest correlation (R² = 0.70) between plant P acquisition with soil rhizosphere biological richness was found in a high P-sorbing soil amended with an organic P source. In the five other situations, the relationships became significant either in soil conditions, with or without mineral P addition, or when the P source was supplied as organic P in the absence of soil, although with a low correlation coefficient (0.09 < R² < 0.15). We thus encourage the systematic and careful consideration of the form and mobility of P in the experimental trials that aim to assess the role of biological complexity on plant P nutrition.
    Keywords Nematoda ; agriculture ; ectomycorrhizae ; growth chambers ; mutualism ; nutrition ; rhizosphere ; soil
    Language English
    Dates of publication 2021-0216
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2651678-0
    ISSN 2077-0472
    ISSN 2077-0472
    DOI 10.3390/agriculture11020157
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Proficient Lignocellulolytic Novel Bacterial Isolates from Diversified Galiyat Forests of Lower Himalaya

    Awan, Malik Owais Ullah / Ak̲h̲tar, Iqbāl / Rashid, Muhammad Imtiaz / Irshad, Usman / Hafeez, Farhan / Ullah, Farid / Irashāda, Muhammada / Ondrasek, Gabrijel / Mustac, Ivan / Nazir, Rashid

    Forests. 2023 June 07, v. 14, no. 6

    2023  

    Abstract: Lignocellulosic biomass needs attention as an alternative energy source to overcome the adverse impacts of fossil fuels. Diversified Galiyat forests of Lower Himalaya may represent the potential source of lignocellulose degrading microbiota, particularly ...

    Abstract Lignocellulosic biomass needs attention as an alternative energy source to overcome the adverse impacts of fossil fuels. Diversified Galiyat forests of Lower Himalaya may represent the potential source of lignocellulose degrading microbiota, particularly the lytic bacteria. Therefore, soil and wood samples were collected from different sites of Nathiagali and Thandiani of Galiyat forests. The soil samples collected were clayey, with a pH between 6.7 and 7.0, and with an organic matter of 2.8%–2.9% in Nathiagali and 2.1%–2.2% in Thandiani. The soils were enriched with more diversified cultivable bacteria (9 Log CFU/g) than the respective wood samples (7.4–8.6 Log CFU/g). Out of 90 bacteria, 22 isolates were efficient for cellulose degradation, 14 for xylanase activity, and 10 for laccase production. Cluster analysis showed that lignocellulolytic bacteria were grouped based on the sample medium (soil–wood) rather than the sampling site (Thandiani–Nathiagali). Efficient bacteria were also sequenced, and we found that cellulase production was prevalent in Pseudomonas spp. while laccase activity was diverse among taxonomically varied bacteria. Moreover, Stenotrophomonas sp. TS2B1 performed the best for corncob xylose degradation. Overall, the results suggest that Galiyat forests represent diverse lignocellulolytic microbial populations which should be further evaluated for applications in lignocellulosic waste management and for potential consequent fuel production.
    Keywords Pseudomonas ; Stenotrophomonas ; biomass ; cellulose ; cluster analysis ; corn cobs ; endo-1,4-beta-glucanase ; fuel production ; laccase ; lignocellulases ; lignocellulose ; lignocellulosic wastes ; microorganisms ; organic matter ; pH ; renewable energy sources ; soil ; waste management ; wood ; xylanases ; xylose ; Himalayan region
    Language English
    Dates of publication 2023-0607
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2527081-3
    ISSN 1999-4907
    ISSN 1999-4907
    DOI 10.3390/f14061180
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Combination of melatonin and plant growth promoting rhizobacteria improved the growth of Spinacia oleracea L. under the arsenic and cadmium stresses

    Asif, Mahnoor / Ahmad, Rafiq / Pervez, Arshid / Al Farraj, Dunia A. / Elshikh, Mohamed S. / Shahzād, Muḥammad / Irshad, Usman / Abbasi, Arshad Mehmood

    Physiological and Molecular Plant Pathology. 2023 Sept., v. 127 p.102097-

    2023  

    Abstract: Arsenic and cadmium are among highly toxic elements that causes detrimental effects to the plants, animals as well as human beings. Melatonin is a natural molecule that works as a strong antioxidant by forming antioxidant cascade reaction by scavenging ... ...

    Abstract Arsenic and cadmium are among highly toxic elements that causes detrimental effects to the plants, animals as well as human beings. Melatonin is a natural molecule that works as a strong antioxidant by forming antioxidant cascade reaction by scavenging free radicals of reactive oxygen species (ROS). In the same way, plant growth promoting rhizobacteria (PGPR) colonize plant roots and enhance plant growth by number of mechanisms. The objective of the current research was to study the impact of melatonin and Bacillus licheniformis in combination on Spinacia oleracea L. in the presence of arsenic (As) and cadmium (Cd) stress. Spinacia oleracea L. seeds were germinated in pots consisting of control and three different doses of arsenic and cadmium irrigated with combination of melatonin and Bacillus licheniformis. The results of the study showed that the combination of melatonin and Bacillus licheniformis has the potential to alleviate the toxic effects of As and Cd. Plant morphological and physiological parameters like plant height, root length, fresh weight, dry weight, chlorophyll content were enhanced because of combine application of melatonin and Bacillus licheniformis. Superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activity increased because of combine application of melatonin and Bacillus licheniformis while malondialdehyde (MDA) content is decreased. In response to different stress conditions, expression of genes encoding the superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) is increased in different As and Cd treatments. Results show that highest translocation factor (TF) 0.44 was observed at 125 mg/kg Cd and highest bioconcentration factor (BCF) 0.46 at 75 mg/kg Cd. All three treatments have TF and BCF less than one thus making Spinacia oleracea L. useful for uptake by human beings. Additionally, it was found the melatonin content in Spinacia oleracea L. was increased under As and Cd stress and exogenous melatonin application.
    Keywords Bacillus licheniformis ; Spinacia oleracea ; arsenic ; bioaccumulation factor ; cadmium ; catalase ; chlorophyll ; humans ; malondialdehyde ; melatonin ; peroxidase ; plant growth ; plant height ; plant pathology ; reactive oxygen species ; rhizosphere bacteria ; superoxide dismutase ; toxicity ; Antioxidants enzymes ; Bioconcentration factor ; Lipid peroxidation ; melatonin quantification ; Spinacia oleracea L., toxic metals ; Translocation factor ; SOD ; POD ; CAT ; MDA ; PGPR ; Cd ; ROS ; H2O2 ; ACC ; TF ; BCF
    Language English
    Dates of publication 2023-09
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 742470-x
    ISSN 0885-5765
    ISSN 0885-5765
    DOI 10.1016/j.pmpp.2023.102097
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Novel microalgae strains from selected lower Himalayan aquatic habitats as potential sources of green products.

    Inuwa, Abdullahi B / Zeb, Iftikhar / Mahmood, Qaisar / Irshad, Usman / Irshad, Muhammad / Hafeez, Farhan / Iqbal, Akhtar / Pervez, Arshid / Nazir, Rashid

    publication RETRACTED

    PloS one

    2022  Volume 17, Issue 5, Page(s) e0267788

    Abstract: Microalgal biomass provides a renewable source of biofuels and other green products. However, in order to realize economically viable microalgal biorefinery, strategic identification and utilization of suitable microalgal feedstock is fundamental. Here, ... ...

    Abstract Microalgal biomass provides a renewable source of biofuels and other green products. However, in order to realize economically viable microalgal biorefinery, strategic identification and utilization of suitable microalgal feedstock is fundamental. Here, a multi-step suboptimal screening strategy was used to target promising microalgae strains from selected freshwaters of the study area. The resulting strains were found to be affiliated to seven closely-related genera of the family Scenedesmaceae, as revealed by both morphologic and molecular characterization. Following initial screening under upper psychrophilic to optimum mesophilic (irregular temperature of 14.1 to 35.9°C) cultivation conditions, superior strains were chosen for further studies. Further cultivation of the selected strains under moderate to extreme mesophilic cultivation conditions (irregular temperature of 25.7 to 42.2°C), yielded up to 74.12 mgL-1day-1, 19.96 mgL-1day-1, 48.56%, 3.34 μg/mL and 1.20 μg/mL, for biomass productivity, lipid productivity, carbohydrate content, pigments content and carotenoids content respectively. These performances were deemed promising compared with some previous, optimum conditions-based reports. Interestingly, the fatty acids profile and the high carotenoids content of the studied strains revealed possible tolerance to the stress caused by the changing suboptimal cultivation conditions. Overall, strains AY1, CM6, LY2 and KL10 were exceptional and may present sustainable, promising feedstock for utilization in large-scale generation of green products, including biodiesel, bioethanol, pigments and dietary supplements. The findings of this study, which exposed promising, eurythermal strains, would expand the current knowledge on the search for promising microalgae strains capable of performing under the largely uncontrolled large-scale cultivation settings.
    MeSH term(s) Biofuels ; Biomass ; Carotenoids ; Chlorophyceae ; Microalgae
    Chemical Substances Biofuels ; Carotenoids (36-88-4)
    Language English
    Publishing date 2022-05-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Retracted Publication
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0267788
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Melatonin and plant growth-promoting rhizobacteria alleviate the cadmium and arsenic stresses and increase the growth of Spinacia oleracea L

    Asif, Mahnoor / Pervez, Arshid / Irshad, Usman / Mehmood, Qaisar / Ahmad, Rafiq

    Plant, Soil and Environment. , v. 66, no. 5

    2020  

    Abstract: Melatonin (N-acetyl-5-methoxytryptamine) is a recently discovered natural product that helps the plant to cope with environmental stresses. In the same way, plant growth-promoting rhizobacteria colonise plant roots and enhance plant stress tolerance. To ... ...

    Abstract Melatonin (N-acetyl-5-methoxytryptamine) is a recently discovered natural product that helps the plant to cope with environmental stresses. In the same way, plant growth-promoting rhizobacteria colonise plant roots and enhance plant stress tolerance. To study the impact of exogenous melatonin and Bacillus licheniformis on the growth of Spinacia oleracea L. seedlings were treated with 100 µmol exogenous melatonin and B. licheniformis under cadmium (Cd) and arsenic (As) stresses by a pot experiment. Different plant growth parameters, antioxidant enzymes, and lipid peroxidation were studied. The results showed that melatonin application and B. licheniformis inoculation alleviated As and Cd toxicity by significantly reducing the negative impacts of stresses and increasing the fresh and dry weight as well as preventing the damage to the chlorophyll content of S. oleracea L. Moreover, supplementation of melatonin, and B. licheniformis, enhanced activities of antioxidant enzymes superoxide dismutase, peroxidase, catalase, thus acting as a line of defense against As and Cd stresses. Similarly, lipid peroxidation was also inhibited by exogenous melatonin and B. licheniformis inoculation. Exogenous application of melatonin and inoculating roots of S. oleracea L. with B. licheniformis found to ameliorate the harmful effects of As and Cd contamination.
    Keywords Bacillus licheniformis ; Spinacia oleracea ; arsenic ; cadmium ; catalase ; chlorophyll ; environment ; lipid peroxidation ; melatonin ; peroxidase ; plant growth ; plant growth-promoting rhizobacteria ; plant stress ; soil ; stress tolerance ; superoxide dismutase ; toxicity
    Language English
    Dates of publication 2020-0525
    Size p. 234-241.
    Publishing place Czech Academy of Agricultural Sciences
    Document type Article
    ZDB-ID 2104321-8
    ISSN 1214-1178
    ISSN 1214-1178
    DOI 10.17221/135/2020-pse
    Database NAL-Catalogue (AGRICOLA)

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