Article ; Online: Exploring the resistome, virulome, and mobilome of multidrug-resistant Klebsiella pneumoniae isolates: deciphering the molecular basis of carbapenem resistance.
2024 Volume 25, Issue 1, Page(s) 408
Abstract: Background: Klebsiella pneumoniae, a notorious pathogen for causing nosocomial infections has become a major cause of neonatal septicemia, leading to high morbidity and mortality worldwide. This opportunistic bacterium has become highly resistant to ... ...
| Abstract | Background: Klebsiella pneumoniae, a notorious pathogen for causing nosocomial infections has become a major cause of neonatal septicemia, leading to high morbidity and mortality worldwide. This opportunistic bacterium has become highly resistant to antibiotics due to the widespread acquisition of genes encoding a variety of enzymes such as extended-spectrum beta-lactamases (ESBLs) and carbapenemases. We collected Klebsiella pneumoniae isolates from a local tertiary care hospital from February 2019-February 2021. To gain molecular insight into the resistome, virulome, and genetic environment of significant genes of multidrug-resistant K. pneumoniae isolates, we performed the short-read whole-genome sequencing of 10 K. pneumoniae isolates recovered from adult patients, neonates, and hospital tap water samples. Results: The draft genomes of the isolates varied in size, ranging from 5.48 to 5.96 Mbp suggesting the genome plasticity of this pathogen. Various genes conferring resistance to different classes of antibiotics e.g., aminoglycosides, quinolones, sulfonamides, tetracycline, and trimethoprim were identified in all sequenced isolates. The highest resistance was observed towards carbapenems, which has been putatively linked to the presence of both class B and class D carbapenemases, bla Conclusions: This study provides valuable insight into the presence of multiple mechanisms of carbapenem resistance in K. pneumoniae strains including the acquisition of multiple antibiotic-resistance genes through mobile genetic elements. Identification of rich mobilome yielded insightful information regarding the crucial role of insertion sequences, transposons, and integrons in shaping the genome of bacteria for the transmission of various resistance-associated genes. Multi-drug resistant isolates that had the fewest resistance genes exhibited a significant number of mutations. K. pneumoniae isolate from water source displayed comparable antibiotic resistance determinants to clinical isolates and the highest number of virulence-associated genes suggesting the possible interplay of ARGs amongst bacteria from different sources. |
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| MeSH term(s) | Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/isolation & purification ; Drug Resistance, Multiple, Bacterial/genetics ; Carbapenems/pharmacology ; Humans ; Whole Genome Sequencing ; Genome, Bacterial ; beta-Lactamases/genetics ; Anti-Bacterial Agents/pharmacology ; Phylogeny ; Klebsiella Infections/microbiology ; Klebsiella Infections/drug therapy ; Microbial Sensitivity Tests ; Bacterial Proteins |
| Chemical Substances | Carbapenems ; beta-Lactamases (EC 3.5.2.6) ; Anti-Bacterial Agents ; carbapenemase (EC 3.5.2.6) ; Bacterial Proteins |
| Language | English |
| Publishing date | 2024-04-25 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2041499-7 |
| ISSN | 1471-2164 ; 1471-2164 |
| ISSN (online) | 1471-2164 |
| ISSN | 1471-2164 |
| DOI | 10.1186/s12864-024-10139-y |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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