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Article: Neuroprotective effects of trigonelline in kainic acid-induced epilepsy: Behavioral, biochemical, and functional insights.

Faizan, Mohammad / Jahan, Iram / Ishaq, Mohd / Alhalmi, Abdulsalam / Khan, Rahmuddin / Noman, Omar M / Hasson, Sidgi / Mothana, Ramzi A

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

2023 Volume 31, Issue 12, Page(s) 101843

Abstract: Trigonelline, an alkaloid found in the seeds ... ...

Abstract	Trigonelline, an alkaloid found in the seeds of
Language	English
Publishing date	2023-10-21
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	1378024-4
ISSN	1319-0164
ISSN	1319-0164
DOI	10.1016/j.jsps.2023.101843
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Article ; Online: Autophagy in cancer: Recent advances and future directions.

Ishaq, Mohd / Ojha, Rani / Sharma, Aditya P / Singh, Shrawan K

Seminars in cancer biology

2020 Volume 66, Page(s) 171–181

Abstract: Autophagy is being explored as a potential therapeutic target for enhancing the cytotoxic effects of chemotherapeutic regimens in various malignancies. Autophagy plays a very important role in cancer pathogenesis. Here, we discuss the updates on the ... ...

Abstract	Autophagy is being explored as a potential therapeutic target for enhancing the cytotoxic effects of chemotherapeutic regimens in various malignancies. Autophagy plays a very important role in cancer pathogenesis. Here, we discuss the updates on the modulation of autophagy via dynamic interactions with different organelles and the exploitation of selective autophagy for exploring therapeutic strategies. We further discuss the role of autophagy inhibitors in cancer preclinical and clinical trials, novel autophagy inhibitors, and challenges likely to be faced by clinicians while inducting autophagy modulators in clinical practice.
MeSH term(s)	Animals ; Antineoplastic Agents/pharmacology ; Autophagy/drug effects ; Autophagy/physiology ; Clinical Trials as Topic ; Drug Evaluation, Preclinical/methods ; Humans ; Molecular Targeted Therapy/methods ; Neoplasms/drug therapy ; Neoplasms/pathology
Chemical Substances	Antineoplastic Agents
Language	English
Publishing date	2020-03-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1033980-2
ISSN	1096-3650 ; 1044-579X
ISSN (online)	1096-3650
ISSN	1044-579X
DOI	10.1016/j.semcancer.2020.03.010
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Article ; Online: Trigonelline Demonstrated Ameliorative Effects in Dexamethasone Induced Osteoporotic Rats.

Rathi, Akshoo / Ishaq, Mohd / Najmi, Abul Kalam / Akhtar, Mohd

Drug research

2020 Volume 70, Issue 6, Page(s) 257–264

Abstract: Aim: Trigonelline is a potent phytochemical present in fenugreek, which has strong anti-oxidant and phytoestrogenic activities. This study was carried out to investigate this estrogenic activity as a possible mechanisms involved in preventing the ... ...

Abstract	Aim: Trigonelline is a potent phytochemical present in fenugreek, which has strong anti-oxidant and phytoestrogenic activities. This study was carried out to investigate this estrogenic activity as a possible mechanisms involved in preventing the symptoms of osteoporosis in dexamethasone induced osteoporosis in rats. Materials and methods: Wistar rats were randomly divided into eight groups, six animals in each group. Osteoporosis was induced using dexamethasone 0.1mg/kg subcutaneously in rats for three times per week for 8 consecutive weeks and treatment with drugs up to 12 weeks as per the treatment schedule described. After 12 weeks, rats were sacrificed; blood samples were collected from each rat and the clear, non hemolysed supernatant sera was used for biochemical examinations. Femurs were used for Bone Mineral Density (BMD), microcomputed tomography (Micro CT), histology and biochemical examinations. Results: BMD, bone micro structure, serum calcium, phosphorus level and serum estradiol levels were decreased while serum PTH levels, SAP and acid phosphatase (ACP) were elevated in dexamethasone treated rats as compared to control (p<0.01). Dexmethasone treated animals showed loss of marrow at multifocal area, cartilage and trabeculae and thinning of trabeculae (bone resorption), zone of cartilage was poorly seen and fat cells in marrow. Trigonelline showed significant improvement and prevent the progression of osteoporosis by enhancing the BMD, restoring bone physiology. Conclusion: Our results confirm the estrogenic activity of triogonelline, which is responsible for its effects; still, it needs further evaluation in other animal models to provide a more conclusive view for its therapeutic usefulness in osteoporosis.
MeSH term(s)	Alkaloids/pharmacology ; Alkaloids/therapeutic use ; Animals ; Bone Density/drug effects ; Dexamethasone/adverse effects ; Disease Models, Animal ; Femur/diagnostic imaging ; Femur/drug effects ; Femur/pathology ; Humans ; Osteoporosis/chemically induced ; Osteoporosis/pathology ; Osteoporosis/prevention & control ; Phytoestrogens/pharmacology ; Phytoestrogens/therapeutic use ; Rats ; Rats, Wistar ; Trigonella/chemistry ; X-Ray Microtomography
Chemical Substances	Alkaloids ; Phytoestrogens ; trigonelline (3NQ9N60I00) ; Dexamethasone (7S5I7G3JQL)
Language	English
Publishing date	2020-04-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2703847-6
ISSN	2194-9387 ; 2194-9379
ISSN (online)	2194-9387
ISSN	2194-9379
DOI	10.1055/a-1147-5724
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Article ; Online: Curcumin Nanoemulsion: Unveiling Cardioprotective Effects via ACE Inhibition and Antioxidant Properties in Hypertensive Rats.

Ishaq, Mohd / Khan, Mohemmed Faraz / Verma, Garima / Rathi, Akshoo / Adil, Mohammad / Faizan, Mohammad / Najmi, Abul Kalam / Akhtar, Mohd / Al Kamaly, Omkulthom / Alshawwa, Samar Zuhair / Shahat, Abdelaaty A / Alhalmi, Abdulsalam

Medicina (Kaunas, Lithuania)

2023 Volume 59, Issue 10

Abstract: Background and ... ...

Abstract	Background and Objectives
MeSH term(s)	Rats ; Animals ; Antioxidants/pharmacology ; Antioxidants/therapeutic use ; Curcumin/pharmacology ; Curcumin/therapeutic use ; Desoxycorticosterone Acetate/adverse effects ; Angiotensin II/adverse effects ; Molecular Docking Simulation ; Rats, Wistar ; Hypertension ; Antihypertensive Agents/therapeutic use ; Blood Pressure
Chemical Substances	Antioxidants ; Curcumin (IT942ZTH98) ; Desoxycorticosterone Acetate (6E0A168OB8) ; Angiotensin II (11128-99-7) ; Antihypertensive Agents
Language	English
Publishing date	2023-09-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2188113-3
ISSN	1648-9144 ; 1010-660X
ISSN (online)	1648-9144
ISSN	1010-660X
DOI	10.3390/medicina59101748
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Article: [No title information]

Rathi, Akshoo / Ishaq, Mohd / Najmi, Abul Kalam / Akhtar, Mohd

Drug Research

2020 Volume 70, Issue 06, Page(s) 257–264

Abstract	Aim: Trigonelline is a potent phytochemical present in fenugreek, which has strong anti-oxidant and phytoestrogenic activities. This study was carried out to investigate this estrogenic activity as a possible mechanisms involved in preventing the symptoms of osteoporosis in dexamethasone induced osteoporosis in rats. Materials and Methods: Wistar rats were randomly divided into eight groups, six animals in each group. Osteoporosis was induced using dexamethasone 0.1mg/kg subcutaneously in rats for three times per week for 8 consecutive weeks and treatment with drugs up to 12 weeks as per the treatment schedule described. After 12 weeks, rats were sacrificed; blood samples were collected from each rat and the clear, non hemolysed supernatant sera was used for biochemical examinations. Femurs were used for Bone Mineral Density (BMD), microcomputed tomography (Micro CT), histology and biochemical examinations. Results: BMD, bone micro structure, serum calcium, phosphorus level and serum estradiol levels were decreased while serum PTH levels, SAP and acid phosphatase (ACP) were elevated in dexamethasone treated rats as compared to control (p<0.01). Dexmethasone treated animals showed loss of marrow at multifocal area, cartilage and trabeculae and thinning of trabeculae (bone resorption), zone of cartilage was poorly seen and fat cells in marrow. Trigonelline showed significant improvement and prevent the progression of osteoporosis by enhancing the BMD, restoring bone physiology. Conclusion: Our results confirm the estrogenic activity of triogonelline, which is responsible for its effects; still, it needs further evaluation in other animal models to provide a more conclusive view for its therapeutic usefulness in osteoporosis.
Keywords	trogonelline ; osteoporosis ; dexamethasone ; bone mineral density ; micro CT
Language	English
Publishing date	2020-04-14
Publisher	© Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	2703847-6
ISSN	2194-9387 ; 2194-9379
ISSN (online)	2194-9387
ISSN	2194-9379
DOI	10.1055/a-1147-5724
Database	Thieme publisher's database

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Article ; Online: Targeting malaria and leishmaniasis: Synthesis and pharmacological evaluation of novel pyrazole-1,3,4-oxadiazole hybrids. Part II.

Verma, Garima / Khan, Mohemmed Faraz / Mohan Nainwal, Lalit / Ishaq, Mohd / Akhter, Mymoona / Bakht, Afroz / Anwer, Tariq / Afrin, Farhat / Islamuddin, Mohammad / Husain, Ibraheem / Alam, Mohammad Mumtaz / Shaquiquzzaman, Mohammad

Bioorganic chemistry

2019 Volume 89, Page(s) 102986

Abstract: In continuance with earlier reported work, an extension has been carried out by the same research group. Mulling over the ongoing condition of resistance to existing antimalarial agents, we had reported synthesis and antimalarial activity of certain ... ...

Abstract	In continuance with earlier reported work, an extension has been carried out by the same research group. Mulling over the ongoing condition of resistance to existing antimalarial agents, we had reported synthesis and antimalarial activity of certain pyrazole-1,3,4-oxadiazole hybrid compounds. Bearing previous results in mind, our research group ideated to design and synthesize some more derivatives with varied substitutions of acetophenone and hydrazide. Following this, derivatives 5a-r were synthesized and tested for antimalarial efficacy by schizont maturation inhibition assay. Further, depending on the literature support and results of our previous series, certain potent compounds (5f, 5n and 5r) were subjected to Falcipain-2 inhibitory assay. Results obtained for these particular compounds further strengthened our hypothesis. Here, in this series, compound 5f having unsubstituted acetophenone part and a furan moiety linked to oxadiazole ring emerged as the most potent compound and results were found to be comparable to that of the most potent compound (indole bearing) of previous series. Additionally, depending on the available literature, compounds (5a-r) were tested for their antileishmanial potential. Compounds 5a, 5c and 5r demonstrated dose-dependent killing of the promastigotes. Their IC
MeSH term(s)	Animals ; Antimalarials/chemistry ; Antimalarials/metabolism ; Antimalarials/pharmacology ; Antiprotozoal Agents/chemical synthesis ; Antiprotozoal Agents/chemistry ; Antiprotozoal Agents/pharmacology ; Binding Sites ; Cell Survival/drug effects ; Cysteine Endopeptidases/chemistry ; Cysteine Endopeptidases/metabolism ; Drug Design ; Inhibitory Concentration 50 ; Leishmania/drug effects ; Leishmania/physiology ; Macrophages/cytology ; Macrophages/metabolism ; Macrophages/parasitology ; Mice ; Molecular Docking Simulation ; Oxadiazoles/chemical synthesis ; Oxadiazoles/chemistry ; Oxadiazoles/pharmacology ; Plasmodium falciparum/drug effects ; Protein Structure, Tertiary ; Pyrazoles/chemistry ; RAW 264.7 Cells ; Rats ; Rats, Wistar ; Structure-Activity Relationship
Chemical Substances	Antimalarials ; Antiprotozoal Agents ; Oxadiazoles ; Pyrazoles ; 1,3,4-oxadiazole (20O2F20OUR) ; pyrazole (3QD5KJZ7ZJ) ; Cysteine Endopeptidases (EC 3.4.22.-) ; falcipain 2 (EC 3.4.22.-)
Language	English
Publishing date	2019-05-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120080-x
ISSN	1090-2120 ; 0045-2068
ISSN (online)	1090-2120
ISSN	0045-2068
DOI	10.1016/j.bioorg.2019.102986
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Article: Resource potential and scope of utilization of renewable energy in Jammu and Kashmir, India

Lohan, Shiv Kumar / Dixit, Jagvir / Modasir, Sheikh / Ishaq, Mohd

Renewable energy. 2012 Mar., v. 39, no. 1

2012

Abstract: Jammu and Kashmir the northern most state of India is blessed with immense potential for utilization of renewable energy. The state at present is fairly untapped in terms of energy utilization and thus venturing into this region would definitely prove to ...

Abstract	Jammu and Kashmir the northern most state of India is blessed with immense potential for utilization of renewable energy. The state at present is fairly untapped in terms of energy utilization and thus venturing into this region would definitely prove to be profitable. The natural energy sources like sunshine, wind, vegetation, water flow, biomass and other biological wastes though abundantly available in the state yet are not being potentially harnessed resulting in very low per capita energy availability, deforestation and poor health. Renewable energy acquires a promising option not only for energy availability next view and of environment protection but also the socio-economic conditions of the people residing in these areas can be improved to a great extent. The hydroelectric power has tremendous potential for generation of electricity in the state because the topography of the state provides extensive network of canals and streams. This paper describes the resource potential and opportunity to enter the market and bring more renewable energy projects in the form of solar, biomass derived fuels, biogas and hydroelectric in the state.
Keywords	biogas ; biomass ; deforestation ; electricity ; energy ; hydroelectric power ; markets ; people ; solar radiation ; streams ; topography ; vegetation ; wastes ; water flow ; wind ; India
Language	English
Dates of publication	2012-03
Size	p. 24-29.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2001449-1
ISSN	0960-1481
ISSN	0960-1481
DOI	10.1016/j.renene.2011.08.033
Database	NAL-Catalogue (AGRICOLA)

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Article: Higher events rate in patients with a normal gated myocardial perfusion imaging with dipyridamole than exercise: "Run for reliability".

Fatima, Nosheen / Uz Zaman, Maseeh / Ishaq, Mohd / Rasheed, S Zahed / Baloch, Dad J / Wali, Asif / Bano, Javeria / Rehman, Kawish

Indian journal of nuclear medicine : IJNM : the official journal of the Society of Nuclear Medicine, India

2013 Volume 27, Issue 3, Page(s) 172–175

Abstract: Objective: The aim of this is to evaluate the negative predictive value (NPV) of a normal gated myocardial perfusion imaging (NGMPI) with exercise and dipyridamole in a propensity matched population.: Materials and methods: This is a prospective ... ...

Abstract	Objective: The aim of this is to evaluate the negative predictive value (NPV) of a normal gated myocardial perfusion imaging (NGMPI) with exercise and dipyridamole in a propensity matched population. Materials and methods: This is a prospective study conducted at Nuclear Cardiology Department of Karachi Institute of Heart Diseases, Karachi from December 2008 until June 2010. A total of 809 patients with a NGMPI with adequate exercise (558/809) or dipyridamole (251/809) stress were included and followed-up for 12-30 months (mean 24 ± 3 months) for fatal or non-fatal myocardial infarctions (MI). Results: Mean ejection fraction (%), end diastolic volume (ml), and end systolic volume (ml) in exercise and dipyridamole cohorts were (72 ± 08, 66 ± 11), (68 ± 13, 81 ± 17), and (19 ± 11, 26 ± 12) respectively. On follow-up, in dipyridamole cohort 2 fatal and 6 non-fatal MIs were reported. While in exercise cohort only 2 non-fatal MIs were reported. The NPV of a NGMPI with exercise was 99.7% (95% confidence interval [CI] 98.93-99.96%) with an event rate of 0.3% (95% CI; 0.03-0.6%) and annualized event rate of 0.15%. The NPV of NGMPI with dipyridamole was 96.80% (95% CI; 2.2-4.3%) with an event rate of 3.2% (95% CI; 1.39-3.83%) and annualized event rate of approximately 1.6%. Event free survival for dipyridamole group was significantly lower than exercise analyzed by Log-rank test (14.509, P < 0.001). Conclusion: A NGMPI with dipyridamole stress has higher event rate (low-NPV) as compared with exercise and this raises concern over its credibility to label these patients into low-risk group.
Language	English
Publishing date	2013-06-28
Publishing country	India
Document type	Journal Article
ZDB-ID	2181722-4
ISSN	0974-0244 ; 0972-3919
ISSN (online)	0974-0244
ISSN	0972-3919
DOI	10.4103/0972-3919.112722
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Article: Interferon-gamma Low producer genotype +874 overrepresented in Bacillus Calmette-Guerin nonresponding children.

Anuradha, Bandaru / Rakh, Shilpa S / Ishaq, Mohd / Murthy, K J R / Valluri, Vijaya Lakshmi

The Pediatric infectious disease journal

2008 Volume 27, Issue 4, Page(s) 325–329

Abstract: Background: There are limited reports on the influence of the immune regulatory genotypes on the efficacy of Bacillus Calmette-Guerin (BCG) in man. This study was designed to evaluate the influence of the cytokine genotype interferon (IFN)-gamma +874T/A ...

Abstract	Background: There are limited reports on the influence of the immune regulatory genotypes on the efficacy of Bacillus Calmette-Guerin (BCG) in man. This study was designed to evaluate the influence of the cytokine genotype interferon (IFN)-gamma +874T/A on T cell in vitro assays in BCG nonresponders (negative to either in vivo or in vitro test with purified protein derivative or both). Methods: Ninety healthy children who were without any clinical evidence of the disease, 45 with a BCG-scar and the remaining 45 without scar were assessed for in vitro T cell responses. CD4+ and CD8+ cell counts were measured by flow cytometry. r32kDaBCG (Ag85A-BCG) protein was used to stimulate T cells and IFN-gamma cytokine concentration in the cultures were measured by enzyme-linked immunosorbent assay. Polymorphism in IFN-gamma (+874T/A) region was detected by amplification refractory mutation system-polymerase chain reaction. Results: T cell subsets were within the normal range in all subjects. Children with TT genotype showed significantly higher antigen-induced IFN-gamma (P < 0.001) as compared with those with AT/AA genotype. The highest values were observed in children with TT genotype combined with positive antigen-specific peripheral blood mononuclear cells proliferation. Seventy-five percent of the vaccinated children with TT genotype showed high amounts of stimulated IFN-gamma compared with 66% of scar negative and 16% of scar positive but with AA genotype. Conclusions: IFN-gamma (+874T/A) polymorphism seemed to be a strong and independent predictor for clinical outcome of both scar-positive and scar-negative children. These results may help in planning future vaccination strategies. The ability to mount in vitro lymphoproliferation did not distinguish the success or failure of BCG vaccination nor predict susceptibility to the disease.
MeSH term(s)	Antigens, Bacterial/immunology ; Cells, Cultured ; Child ; Child, Preschool ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Gene Frequency ; Genotype ; Humans ; Infant ; Infant, Newborn ; Interferon-gamma/biosynthesis ; Interferon-gamma/genetics ; Lymphocyte Activation ; Lymphocyte Count ; Mycobacterium bovis/immunology ; Polymerase Chain Reaction/methods ; Polymorphism, Genetic ; T-Lymphocyte Subsets/immunology ; T-Lymphocytes/immunology ; Tuberculosis/genetics ; Tuberculosis/immunology ; Tuberculosis/prevention & control ; Tuberculosis Vaccines/immunology
Chemical Substances	Antigens, Bacterial ; Tuberculosis Vaccines ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2008-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	392481-6
ISSN	1532-0987 ; 0891-3668
ISSN (online)	1532-0987
ISSN	0891-3668
DOI	10.1097/INF.0b013e31816099e6
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Article: DNA damage in leukocytes of mice treated with copper sulfate.

Saleha Banu, B / Ishaq, Mohd / Danadevi, K / Padmavathi, P / Ahuja, Y R

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

2004 Volume 42, Issue 12, Page(s) 1931–1936

Abstract: Single stranded DNA breaks induced by copper sulfate (CuSO(4)) in mice has been studied in vivo using Alkaline Single Cell Gel Electrophoresis (Comet assay). Mice were administered orally with doses of 0, 1.25, 2.50, 5.00, 7.50, 10.00 and 12.50 mg/kg ... ...

Abstract	Single stranded DNA breaks induced by copper sulfate (CuSO(4)) in mice has been studied in vivo using Alkaline Single Cell Gel Electrophoresis (Comet assay). Mice were administered orally with doses of 0, 1.25, 2.50, 5.00, 7.50, 10.00 and 12.50 mg/kg body weight (b.wt.) of CuSO4 respectively. The samples of whole blood were collected at 24, 48, 72 h, first week and second week post-treatment and the assay was carried out to determine single strand DNA breaks as represented by comet tail-length. In addition, the sample was used to study the repair efficiency by incubating the samples with RPMI medium for 2 h. Results indicated a significant DNA damage at all the doses after treatment with CuSO4 when compared to controls showing a clear dose-dependent response (p < 0.05). A gradual decrease in the tail-lengths from 48 h post-treatment was observed and by second week, the values returned to control levels at all doses. The study on the repair efficiency indicated that mice treated with all the doses of CuSO4 showed decrease in mean comet tail-length indicating repair efficiency capacity but less when compared to those of controls. The study also reveals that comet assay is a sensitive and rapid method for detecting DNA damage caused by trace metals such as copper (Cu).
MeSH term(s)	Animals ; Cell Survival/drug effects ; Cells, Cultured ; Comet Assay ; Copper Sulfate/toxicity ; DNA Damage/drug effects ; Fungicides, Industrial/toxicity ; Leukocytes/drug effects ; Male ; Mice ; Sepharose ; Trypan Blue
Chemical Substances	Fungicides, Industrial ; Sepharose (9012-36-6) ; Trypan Blue (I2ZWO3LS3M) ; Copper Sulfate (LRX7AJ16DT)
Language	English
Publishing date	2004-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2004.07.007
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