LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 2 of total 2

Search options

  1. Article ; Online: Microscopic Detection of DNA Synthesis in Early Mitosis at Repetitive

    Yoshida, Kazumasa / Ishimoto, Riko / Fujita, Masatoshi

    Bio-protocol

    2022  Volume 12, Issue 17

    Abstract: In the human cell cycle, complete replication of DNA is a fundamental process for the maintenance of genome integrity. Replication stress interfering with the progression of replication forks causes difficult-to-replicate regions to remain under- ... ...

    Abstract In the human cell cycle, complete replication of DNA is a fundamental process for the maintenance of genome integrity. Replication stress interfering with the progression of replication forks causes difficult-to-replicate regions to remain under-replicated until the onset of mitosis. In early mitosis, a homology-directed repair DNA synthesis, called mitotic DNA synthesis (MiDAS), is triggered to complete DNA replication. Here, we present a method to detect MiDAS in human U2OS 40-2-6 cells, in which repetitive
    Language English
    Publishing date 2022-09-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4504
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: SLX4-XPF mediates DNA damage responses to replication stress induced by DNA-protein interactions.

    Ishimoto, Riko / Tsuzuki, Yota / Matsumura, Tomoki / Kurashige, Seiichiro / Enokitani, Kouki / Narimatsu, Koki / Higa, Mitsunori / Sugimoto, Nozomi / Yoshida, Kazumasa / Fujita, Masatoshi

    The Journal of cell biology

    2020  Volume 220, Issue 1

    Abstract: The DNA damage response (DDR) has a critical role in the maintenance of genomic integrity during chromosome replication. However, responses to replication stress evoked by tight DNA-protein complexes have not been fully elucidated. Here, we used ... ...

    Abstract The DNA damage response (DDR) has a critical role in the maintenance of genomic integrity during chromosome replication. However, responses to replication stress evoked by tight DNA-protein complexes have not been fully elucidated. Here, we used bacterial LacI protein binding to lacO arrays to make site-specific replication fork barriers on the human chromosome. These barriers induced the accumulation of single-stranded DNA (ssDNA) and various DDR proteins at the lacO site. SLX4-XPF functioned as an upstream factor for the accumulation of DDR proteins, and consequently, ATR and FANCD2 were interdependently recruited. Moreover, LacI binding in S phase caused underreplication and abnormal mitotic segregation of the lacO arrays. Finally, we show that the SLX4-ATR axis represses the anaphase abnormality induced by LacI binding. Our results outline a long-term process by which human cells manage nucleoprotein obstacles ahead of the replication fork to prevent chromosomal instability.
    MeSH term(s) Anaphase ; Ataxia Telangiectasia Mutated Proteins/metabolism ; Cell Line, Tumor ; Chromosome Segregation ; Chromosomes, Human/metabolism ; DNA/metabolism ; DNA Damage ; DNA Replication ; DNA-Binding Proteins/metabolism ; Fanconi Anemia Complementation Group D2 Protein/metabolism ; Humans ; Models, Biological ; Protein Binding ; Recombinases/metabolism ; S Phase ; Stress, Physiological
    Chemical Substances DNA-Binding Proteins ; Fanconi Anemia Complementation Group D2 Protein ; Recombinases ; xeroderma pigmentosum group F protein ; DNA (9007-49-2) ; ATR protein, human (EC 2.7.11.1) ; Ataxia Telangiectasia Mutated Proteins (EC 2.7.11.1) ; SLX4 protein, human (EC 3.1.-)
    Language English
    Publishing date 2020-12-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218154-x
    ISSN 1540-8140 ; 0021-9525
    ISSN (online) 1540-8140
    ISSN 0021-9525
    DOI 10.1083/jcb.202003148
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ub I Zs.190: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top