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  1. AU="Ishizaki, Kimihiro"
  2. AU="Sharma, Shuchita"
  3. AU="Mishra, Tanushree"
  4. AU=Chemes H E
  5. AU="Wang, Yuguang"
  6. AU="Wu, Sheng-Hua"
  7. AU="Irwin, Brian"
  8. AU="Vizcaíno-Velázquez, Pilar"
  9. AU="Cairncross, J Gregory"
  10. AU=Small Eusebius
  11. AU="Poppelaars, Marlou"
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  13. AU="Tapias, Carlos"
  14. AU=Kojima Shihoko
  15. AU="Alcahut-Rodríguez, Cristian"
  16. AU="Whiteside, Theresa L"
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  27. AU="Colón-Ramos, Daniel A" AU="Colón-Ramos, Daniel A"
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  35. AU="London, Kevin"
  36. AU=Pain Debkumar
  37. AU="Snider, Elizabeth"
  38. AU="Klein, J R"
  39. AU="Deslandes, K."
  40. AU="Xie, Dandan"
  41. AU=Ward Helen
  42. AU="Grace Deukmedjian"
  43. AU="Lindeman, Neal"
  44. AU="Okoli, Mary"
  45. AU="Mathew, Philip"
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  1. Artikel ; Online: Scanning ion conductance microscopy of isolated metaphase chromosomes in a liquid environment.

    Ushiki, Tatsuo / Ishizaki, Kimihiro / Mizutani, Yusuke / Nakajima, Masato / Iwata, Futoshi

    Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

    2021  Band 29, Heft 1, Seite(n) 95–106

    Abstract: Scanning probe microscopy (SPM) uses a probing tip which scans over a sample surface for obtaining information on the sample surface characteristics. Among various types of SPM, atomic force microscopy (AFM) has been widely applied to imaging of ... ...

    Abstract Scanning probe microscopy (SPM) uses a probing tip which scans over a sample surface for obtaining information on the sample surface characteristics. Among various types of SPM, atomic force microscopy (AFM) has been widely applied to imaging of biological samples including chromosomes. Scanning ion conductance microscopy (SICM) has been also introduced for visualizing the surface structure of biological samples because it can obtain "contact-free" topographic images in liquid conditions by detecting ion current flow through a pipette opening. However, we recently noticed that the consistent imaging of chromosomes is difficult by SICM. In this paper, the behaviors of the ion current on the sample surfaces were precisely investigated for obtaining SICM images of isolated muntjac metaphase chromosomes more consistently than at present. The present study revealed that application of positive potential to the pipette electrode was acceptable for obtaining the topographic image of chromosomes, while application of negative potential failed in imaging. The approach curves were then studied for analyzing the relationship between the ion current and the tip sample distance when the pipette is approaching chromosomes. The current-voltage (I-V) curve further provided us the accurate interpretation of the ion current behavior during chromosome imaging. These data were further compared with those for SICM imaging of HeLa cells. Our findings indicated that chromosomes are electrically charged and the net charge is strongly negative in normal Dulbecco's phosphate buffered saline. We finally showed that the ion concentration of the bath electrolyte is important for imaging chromosomes by SICM.
    Mesh-Begriff(e) Chromosomes ; HeLa Cells ; Humans ; Metaphase ; Microscopy
    Sprache Englisch
    Erscheinungsdatum 2021-03-11
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1161632-5
    ISSN 1573-6849 ; 0967-3849
    ISSN (online) 1573-6849
    ISSN 0967-3849
    DOI 10.1007/s10577-021-09659-0
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 3873: Hefte anzeigen Standort:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Artikel: Scanning ion conductance microscopy of isolated metaphase chromosomes in a liquid environment

    Ushiki, Tatsuo / Ishizaki, Kimihiro / Mizutani, Yusuke / Nakajima, Masato / Iwata, Futoshi

    Chromosome research. 2021 Mar., v. 29, no. 1

    2021  

    Abstract: Scanning probe microscopy (SPM) uses a probing tip which scans over a sample surface for obtaining information on the sample surface characteristics. Among various types of SPM, atomic force microscopy (AFM) has been widely applied to imaging of ... ...

    Abstract Scanning probe microscopy (SPM) uses a probing tip which scans over a sample surface for obtaining information on the sample surface characteristics. Among various types of SPM, atomic force microscopy (AFM) has been widely applied to imaging of biological samples including chromosomes. Scanning ion conductance microscopy (SICM) has been also introduced for visualizing the surface structure of biological samples because it can obtain “contact-free” topographic images in liquid conditions by detecting ion current flow through a pipette opening. However, we recently noticed that the consistent imaging of chromosomes is difficult by SICM. In this paper, the behaviors of the ion current on the sample surfaces were precisely investigated for obtaining SICM images of isolated muntjac metaphase chromosomes more consistently than at present. The present study revealed that application of positive potential to the pipette electrode was acceptable for obtaining the topographic image of chromosomes, while application of negative potential failed in imaging. The approach curves were then studied for analyzing the relationship between the ion current and the tip sample distance when the pipette is approaching chromosomes. The current-voltage (I-V) curve further provided us the accurate interpretation of the ion current behavior during chromosome imaging. These data were further compared with those for SICM imaging of HeLa cells. Our findings indicated that chromosomes are electrically charged and the net charge is strongly negative in normal Dulbecco’s phosphate buffered saline. We finally showed that the ion concentration of the bath electrolyte is important for imaging chromosomes by SICM.
    Schlagwörter atomic force microscopy ; chromosomes ; electrodes ; electrolytes ; liquids ; metaphase ; phosphates ; research
    Sprache Englisch
    Erscheinungsverlauf 2021-03
    Umfang p. 95-106.
    Erscheinungsort Springer Netherlands
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 1161632-5
    ISSN 1573-6849 ; 0967-3849
    ISSN (online) 1573-6849
    ISSN 0967-3849
    DOI 10.1007/s10577-021-09659-0
    Datenquelle NAL Katalog (AGRICOLA)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 3873: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

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