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  1. AU="Ismail, Mohd Iswadi"
  2. AU=Bian Jin-Song
  3. AU="Perez-Gonzaga, Sergio"
  4. AU=Sathiyamurthy Sundar
  5. AU="Ananth, Kirtana"
  6. AU="Amigó, Universidad Católica Luis"
  7. AU="Algahtani, Norah"
  8. AU=Hidayat Rakhmad
  9. AU="Siebler, Margot"
  10. AU=Schubert Katharina AU=Schubert Katharina
  11. AU="Barakat, Sarah"
  12. AU="Turchetti, Giovanni"
  13. AU="Floramo, Emily" AU="Floramo, Emily"
  14. AU="Wallrabenstein, H"
  15. AU="Ho, Carmen Lok Tung"
  16. AU=Wertheim Jason A
  17. AU="Steele, Joshua A."
  18. AU="Behnampour, Naser"
  19. AU="Stucki, Brenton"
  20. AU="J. Lohse"
  21. AU="Lopez-Plaza, Ileana"
  22. AU="Degorce, Sebastien L"
  23. AU="Wodchis, Walter"
  24. AU="Charles DeCarli"
  25. AU=Glied Sherry
  26. AU="Kesarwani, Manoj"
  27. AU="Cubas-Atienza, Ana I"
  28. AU="Thickens, Anna S"
  29. AU="Anderson, Emilie"
  30. AU=Starace Fabrizio
  31. AU="Ochin, Evelina"
  32. AU="Gijzen, Linda"
  33. AU="van Groeningen, Kees Jan"
  34. AU="Pakserian, Diana"
  35. AU="Joly-Chevrier, Maxine"
  36. AU="Genovesi, Piero"
  37. AU="Ersöz, Gülden"
  38. AU="Jeffrey J Babon"
  39. AU="Rehana Masood"
  40. AU="Sandra Heskamp"
  41. AU="Omid Sadeghi"
  42. AU="Antaya, Richard"
  43. AU="Papadopoulos, G"
  44. AU="Boughen, Santiago"
  45. AU="Brink, P. L."

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  1. Artikel: Characterization of S1 gene sequence variations of attenuated QX-like and variant infectious bronchitis virus strains and the pathogenicity of the viruses in specific-pathogen-free chickens

    Ismail, Mohd Iswadi / Wei, Tan Sheau / Hair-Bejo, Mohd / Omar, Abdul Rahman

    Archives of virology. 2020 Dec., v. 165, no. 12

    2020  

    Abstract: Besides the vaccine strains, the Malaysian variant (MV) and QX-like are the predominant IBVs detected on commercial poultry farms. These two virus strains are distinct based on genomic and pathogenicity studies. In this study, we determined the sequence ... ...

    Abstract Besides the vaccine strains, the Malaysian variant (MV) and QX-like are the predominant IBVs detected on commercial poultry farms. These two virus strains are distinct based on genomic and pathogenicity studies. In this study, we determined the sequence of the S1 gene and compared the pathogenicity of serial passage 70 (P70) of Malaysian QX-like (QX/P70) and MV (MV/P70) strains with that of their respective wild-type viruses. The nucleotide and amino acid sequences of the complete S1 genes of QX/P70 and MV/P70 showed 1.4 to 1.6% and 3.0 to 3.3% variation, respectively, when compared to the wild-type virus. Most of the mutations were insertions and substitutions in the hypervariable regions (HVRs), primarily in HVR 3. Furthermore, selection pressure analysis showed that both viruses are under purifying selection. A pathogenicity study in specific-pathogen-free (SPF) chickens showed a reduction in respiratory and kidney lesions in chickens inoculated with MV/P70, but not with QX/P70, when compared to the respective wild-type viruses. However, MV/P70 is still pathogenic and can cause ciliary damage. In conclusion, the MV IBV strain is more responsive than the QX-like IBV strain following the attenuation process used for the development of a live attenuated IBV vaccine.
    Schlagwörter Infectious bronchitis virus ; chickens ; farms ; genes ; genomics ; kidneys ; live vaccines ; mutation ; nucleotide sequences ; pathogenicity ; selection pressure ; specific pathogen-free animals ; strains ; virology ; viruses
    Sprache Englisch
    Erscheinungsverlauf 2020-12
    Umfang p. 2777-2788.
    Erscheinungsort Springer Vienna
    Dokumenttyp Artikel
    Anmerkung NAL-light
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-020-04812-2
    Datenquelle NAL Katalog (AGRICOLA)

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    Verfügbar in ZB MED Bonn

    Z 70/128: Hefte anzeigen

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  2. Artikel ; Online: Characterization of S1 gene sequence variations of attenuated QX-like and variant infectious bronchitis virus strains and the pathogenicity of the viruses in specific-pathogen-free chickens.

    Ismail, Mohd Iswadi / Wei, Tan Sheau / Hair-Bejo, Mohd / Omar, Abdul Rahman

    Archives of virology

    2020  Band 165, Heft 12, Seite(n) 2777–2788

    Abstract: Besides the vaccine strains, the Malaysian variant (MV) and QX-like are the predominant IBVs detected on commercial poultry farms. These two virus strains are distinct based on genomic and pathogenicity studies. In this study, we determined the sequence ... ...

    Abstract Besides the vaccine strains, the Malaysian variant (MV) and QX-like are the predominant IBVs detected on commercial poultry farms. These two virus strains are distinct based on genomic and pathogenicity studies. In this study, we determined the sequence of the S1 gene and compared the pathogenicity of serial passage 70 (P70) of Malaysian QX-like (QX/P70) and MV (MV/P70) strains with that of their respective wild-type viruses. The nucleotide and amino acid sequences of the complete S1 genes of QX/P70 and MV/P70 showed 1.4 to 1.6% and 3.0 to 3.3% variation, respectively, when compared to the wild-type virus. Most of the mutations were insertions and substitutions in the hypervariable regions (HVRs), primarily in HVR 3. Furthermore, selection pressure analysis showed that both viruses are under purifying selection. A pathogenicity study in specific-pathogen-free (SPF) chickens showed a reduction in respiratory and kidney lesions in chickens inoculated with MV/P70, but not with QX/P70, when compared to the respective wild-type viruses. However, MV/P70 is still pathogenic and can cause ciliary damage. In conclusion, the MV IBV strain is more responsive than the QX-like IBV strain following the attenuation process used for the development of a live attenuated IBV vaccine.
    Mesh-Begriff(e) Animals ; Chickens ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Infectious bronchitis virus/genetics ; Infectious bronchitis virus/pathogenicity ; Poultry Diseases/pathology ; Poultry Diseases/prevention & control ; Poultry Diseases/virology ; Sequence Analysis, Protein ; Serial Passage ; Specific Pathogen-Free Organisms ; Vaccines, Attenuated/immunology
    Chemische Substanzen Vaccines, Attenuated
    Sprache Englisch
    Erscheinungsdatum 2020-09-22
    Erscheinungsland Austria
    Dokumenttyp Journal Article
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-020-04812-2
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Ud II Zs.110: Hefte anzeigen Standort:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Artikel ; Online: Evaluation of the antigen relatedness and efficacy of a single vaccination with different infectious bronchitis virus strains against a challenge with Malaysian variant and QX-like IBV strains.

    Ismail, Mohd Iswadi / Tan, Sheau Wei / Hair-Bejo, Mohd / Omar, Abdul Rahman

    Journal of veterinary science

    2020  Band 21, Heft 6, Seite(n) e76

    Abstract: Background: The predominant infectious bronchitis virus (IBV) strains detected in chickens in Malaysia are the Malaysian variant (MV) and QX-like, which are associated with respiratory distress, nephropathy, and high mortality. On the other hand, the ... ...

    Abstract Background: The predominant infectious bronchitis virus (IBV) strains detected in chickens in Malaysia are the Malaysian variant (MV) and QX-like, which are associated with respiratory distress, nephropathy, and high mortality. On the other hand, the antigenic relatedness and efficacy of IBV vaccines against these 2 field IBV strains are not well characterized.
    Objectives: This study aimed to determine the antigen relatedness and efficacy of different IB vaccine strains against a challenge with MV and QX-like strains.
    Methods: The antigen relatedness and the ability of different IB vaccine strains in conferring protection against MV and QX-like were assessed based on the clinical signs, macroscopic lesions, and ciliary activity.
    Results: The MV strain IBS037A/2014 showed minor antigenic subtype differences with the vaccine virus Mass H120 and 4/91 strains but showed major antigenic subtype differences with the K2 strain. The Malaysian QX-like strain IBS130/2015 showed major antigenic subtype differences with the MV strain IBS037A/2014 and the vaccine strains except for K2. Chickens vaccinated once with Mass (H120) or with non-Mass (4/91 and K2) developed antibody responses with the highest antibody titer detected in the groups vaccinated with H120 and 4/91. The mean ciliary activities of the vaccinated chickens were between 56 to 59% and 48 to 52% in chickens challenged with IBS037A/2014 and IBS130/2015, respectively. The vaccinated and challenged birds showed mild to severe lesions in the lungs and kidneys.
    Conclusions: Despite the minor antigenic subtype differences, a single inoculation with Mass or non-Mass vaccines could not protect against the MV IBS037A/2014 and QX-like IBS130/2015.
    Mesh-Begriff(e) Animals ; Chickens ; Coronavirus Infections/immunology ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Infectious bronchitis virus/immunology ; Malaysia ; Poultry Diseases/immunology ; Poultry Diseases/virology ; Specific Pathogen-Free Organisms ; Vaccination/veterinary ; Vaccines, Attenuated/administration & dosage ; Viral Vaccines/administration & dosage
    Chemische Substanzen Vaccines, Attenuated ; Viral Vaccines
    Sprache Englisch
    Erscheinungsdatum 2020-12-02
    Erscheinungsland Korea (South)
    Dokumenttyp Journal Article ; Randomized Controlled Trial, Veterinary
    ZDB-ID 2125308-0
    ISSN 1976-555X ; 1976-555X
    ISSN (online) 1976-555X
    ISSN 1976-555X
    DOI 10.4142/jvs.2020.21.e76
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Prevalence of Muscular Sarcosporidiosis in Slaughtered Domestic Pigs in Perak, Peninsular Malaysia.

    Zainalabidin, Fazly Ann / Noorazmi, Muhamad Syamsul Naim / Bakri, Wan Normaziah Wan Omar / Sathaya, Geethamalar / Ismail, Mohd Iswadi

    Tropical life sciences research

    2016  Band 28, Heft 1, Seite(n) 161–166

    Abstract: Sarcosporidiosis is a disease caused by intracellular protozoan parasites, namely, ...

    Abstract Sarcosporidiosis is a disease caused by intracellular protozoan parasites, namely,
    Sprache Englisch
    Erscheinungsdatum 2016-12-27
    Erscheinungsland Malaysia
    Dokumenttyp Journal Article
    ZDB-ID 2607038-8
    ISSN 2180-4249 ; 1985-3718
    ISSN (online) 2180-4249
    ISSN 1985-3718
    DOI 10.21315/tlsr2017.28.1.12
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: Screening for Zoonotic Fascioliasis in Slaughtered Large Ruminants in Abattoirs in Perak, Malaysia.

    Zainalabidin, Fazly Ann / Azmi, Muhamad Syamsul Naim Noor / Bakri, Wan Normaziah Wan Omar / Sathaya, Geethamalar / Ismail, Mohd Iswadi

    Tropical life sciences research

    2016  Band 26, Heft 2, Seite(n) 121–124

    Abstract: Fascioliasis, or trematode infestation, is an important disease caused by Fasciola hepatica and Fasciola gigantica. Both species are hepatic parasites that affect humans. We have examined the zoonotic aspects of fascioliasis. A total of 80 fresh liver ... ...

    Abstract Fascioliasis, or trematode infestation, is an important disease caused by Fasciola hepatica and Fasciola gigantica. Both species are hepatic parasites that affect humans. We have examined the zoonotic aspects of fascioliasis. A total of 80 fresh liver samples were collected from 67 Kedah-Kelantan crossbred cattle and 13 Murrah buffalo at 4 local abattoirs in Perak, Malaysia. The samples were examined macroscopically to detect the presence of Fasciola spp. The results show 7.50% (6 of 80) of the animals were diagnosed with fascioliasis. Overall, 7.46% (5 of 67) and 7.69% (1 of 13) of cattle and buffalo samples were positive, respectively. There were only F. gigantica species identified in the samples. Our findings suggest that precautions should be taken because the disease has a zoonotic impact on public health.
    Sprache Englisch
    Erscheinungsdatum 2016-02-12
    Erscheinungsland Malaysia
    Dokumenttyp Journal Article
    ZDB-ID 2607038-8
    ISSN 2180-4249 ; 1985-3718
    ISSN (online) 2180-4249
    ISSN 1985-3718
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: The prevalence of parasitic infestation of small ruminant farms in perak, malaysia.

    Zainalabidin, Fazly Ann / Raimy, Nurulaini / Yaacob, Muhamad Hazmi / Musbah, Adnan / Bathmanaban, Premalaatha / Ismail, Erwanas Asmar / Mamat, Zaini Che / Zahari, Zawida / Ismail, Mohd Iswadi / Panchadcharam, Chandrawathani

    Tropical life sciences research

    2014  Band 26, Heft 1, Seite(n) 1–8

    Abstract: Helminthiasis due to strongyles such as Haemonchus contortus, coccidiosis caused by Eimeria sp. and blood parasite diseases such as theileriosis by Theileria sp. have been reported to cause severe morbidity and mortality annually in small ruminants in ... ...

    Abstract Helminthiasis due to strongyles such as Haemonchus contortus, coccidiosis caused by Eimeria sp. and blood parasite diseases such as theileriosis by Theileria sp. have been reported to cause severe morbidity and mortality annually in small ruminants in Malaysia. The aims of this study were to investigate the prevalence of helminthiasis, coccidiosis and theileriosis and to determine the packed cell volume (PCV) value of small ruminants in Perak, Malaysia. Blood and faecal samples were obtained from a total of 175 animals from 7 small ruminant farms in Kampar, Larut Matang and Selama, Kuala Kangsar and Manjung districts in Perak; the samples were examined for parasitic infestations from April to July 2011. The results of this study show that H. contortus was found in 152 (86.86%) animals, Eimeria sp. was found in 162 (92.57%) animals and the blood protozoa Theileria sp. was found in 25 (14.30%) animals. The PCV values of all of these animals were recorded between 7% and 44%. A total of 42 (24%) animals were anaemic, with a PCV of less than 21%. Continuous monitoring of small ruminant farms will provide important information for assisting farmers with managing the spread of parasitic infections and maintaining the productivity of animals.
    Sprache Englisch
    Erscheinungsdatum 2014-05-13
    Erscheinungsland Malaysia
    Dokumenttyp Journal Article
    ZDB-ID 2607038-8
    ISSN 2180-4249 ; 1985-3718
    ISSN (online) 2180-4249
    ISSN 1985-3718
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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