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  1. Article ; Online: Plant Defense and Viral Counter-Defense during Plant-Geminivirus Interactions.

    Zhang, Jianhang / Ma, Mengyuan / Liu, Yule / Ismayil, Asigul

    Viruses

    2023  Volume 15, Issue 2

    Abstract: Geminiviruses are the largest family of plant viruses that cause severe diseases and devastating yield losses of economically important crops worldwide. In response to geminivirus infection, plants have evolved ingenious defense mechanisms to diminish or ...

    Abstract Geminiviruses are the largest family of plant viruses that cause severe diseases and devastating yield losses of economically important crops worldwide. In response to geminivirus infection, plants have evolved ingenious defense mechanisms to diminish or eliminate invading viral pathogens. However, increasing evidence shows that geminiviruses can interfere with plant defense response and create a suitable cell environment by hijacking host plant machinery to achieve successful infections. In this review, we discuss recent findings about plant defense and viral counter-defense during plant-geminivirus interactions.
    MeSH term(s) Geminiviridae ; Crops, Agricultural
    Language English
    Publishing date 2023-02-12
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15020510
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Autophagy in Plant-Virus Interactions.

    Yang, Meng / Ismayil, Asigul / Liu, Yule

    Annual review of virology

    2020  Volume 7, Issue 1, Page(s) 403–419

    Abstract: Autophagy is a conserved vacuole/lysosome-mediated degradation pathway for clearing and recycling cellular components including cytosol, macromolecules, and dysfunctional organelles. In recent years, autophagy has emerged to play important roles in plant- ...

    Abstract Autophagy is a conserved vacuole/lysosome-mediated degradation pathway for clearing and recycling cellular components including cytosol, macromolecules, and dysfunctional organelles. In recent years, autophagy has emerged to play important roles in plant-pathogen interactions. It acts as an antiviral defense mechanism in plants. Moreover, increasing evidence shows that plant viruses can manipulate, hijack, or even exploit the autophagy pathway to promote pathogenesis, demonstrating the pivotal role of autophagy in the evolutionary arms race between hosts and viruses. In this review, we discuss recent findings about the antiviral and proviral roles of autophagy in plant-virus interactions.
    MeSH term(s) Autophagy ; Host-Pathogen Interactions ; Organelles ; Plant Viruses/classification ; Plant Viruses/pathogenicity ; Plants/virology
    Language English
    Publishing date 2020-06-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2764224-0
    ISSN 2327-0578 ; 2327-056X
    ISSN (online) 2327-0578
    ISSN 2327-056X
    DOI 10.1146/annurev-virology-010220-054709
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Role of autophagy during plant-virus interactions.

    Ismayil, Asigul / Yang, Meng / Liu, Yule

    Seminars in cell & developmental biology

    2019  Volume 101, Page(s) 36–40

    Abstract: Autophagy is an essential and conserved cellular degradation pathway in eukaryotes. In metazoans, autophagy is highly engaged during the immune responses through interfacing either directly with intracellular pathogens or indirectly with immune signaling ...

    Abstract Autophagy is an essential and conserved cellular degradation pathway in eukaryotes. In metazoans, autophagy is highly engaged during the immune responses through interfacing either directly with intracellular pathogens or indirectly with immune signaling molecules. Recent studies have demonstrated that autophagy plays important roles in regulating immunity-related cell death, antiviral and promoting viral pathogenesis during plant-virus interactions. In this review, we will summarize latest progresses and discuss the significant roles of autophagy in the defense and counter-defense arm race between host plants and viruses.
    MeSH term(s) Autophagy/immunology ; Host-Pathogen Interactions/immunology ; Plant Viruses/immunology ; Plants/immunology ; Plants/virology
    Language English
    Publishing date 2019-07-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1312473-0
    ISSN 1096-3634 ; 1084-9521
    ISSN (online) 1096-3634
    ISSN 1084-9521
    DOI 10.1016/j.semcdb.2019.07.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Cotton leaf curl Multan virus C4 protein suppresses autophagy to facilitate viral infection.

    Yang, Meng / Ismayil, Asigul / Gao, Teng / Ye, Zihan / Yue, Ning / Wu, Jie / Zheng, Xiyin / Li, Yiqing / Wang, Yan / Hong, Yiguo / Liu, Yule

    Plant physiology

    2023  Volume 193, Issue 1, Page(s) 708–720

    Abstract: Autophagy plays an important role in plant antiviral defense. Several plant viruses are reported to encode viral suppressor of autophagy (VSA) to prevent autophagy for effective virus infection. However, whether and how other viruses, in particular DNA ... ...

    Abstract Autophagy plays an important role in plant antiviral defense. Several plant viruses are reported to encode viral suppressor of autophagy (VSA) to prevent autophagy for effective virus infection. However, whether and how other viruses, in particular DNA viruses, also encode VSAs to affect viral infection in plants is unknown. Here, we report that the C4 protein encoded by Cotton leaf curl Multan geminivirus (CLCuMuV) inhibits autophagy by binding to the autophagy negative regulator eukaryotic translation initiation factor 4A (eIF4A) to enhance the eIF4A-Autophagy-related protein 5 (ATG5) interaction. By contrast, the R54A or R54K mutation in C4 abolishes its capacity to interact with eIF4A, and neither C4R54A nor C4R54K can suppress autophagy. However, the R54 residue is not essential for C4 to interfere with transcriptional gene silencing or post-transcriptional gene silencing. Moreover, plants infected with mutated CLCuMuV-C4R54K develop less severe symptoms with decreased levels of viral DNA. These findings reveal a molecular mechanism underlying how the DNA virus CLCuMuV deploys a VSA to subdue host cellular antiviral autophagy defense and uphold viral infection in plants.
    MeSH term(s) Nicotiana/genetics ; Begomovirus/genetics ; Proteins/metabolism ; DNA, Viral/genetics ; DNA, Viral/metabolism ; Virus Diseases ; Autophagy/genetics ; Antiviral Agents/metabolism ; Plant Diseases
    Chemical Substances Proteins ; DNA, Viral ; Antiviral Agents
    Language English
    Publishing date 2023-04-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1093/plphys/kiad235
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A viral protein disrupts vacuolar acidification to facilitate virus infection in plants.

    Yang, Meng / Ismayil, Asigul / Jiang, Zhihao / Wang, Yan / Zheng, Xiyin / Yan, Liming / Hong, Yiguo / Li, Dawei / Liu, Yule

    The EMBO journal

    2021  Volume 41, Issue 2, Page(s) e108713

    Abstract: Vacuolar acidification is essential for vacuoles in diverse physiological functions. However, its role in plant defense, and whether and how pathogens affect vacuolar acidification to promote infection remain unknown. Here, we show that Barley stripe ... ...

    Abstract Vacuolar acidification is essential for vacuoles in diverse physiological functions. However, its role in plant defense, and whether and how pathogens affect vacuolar acidification to promote infection remain unknown. Here, we show that Barley stripe mosaic virus (BSMV) replicase γa, but not its mutant γa
    MeSH term(s) Plant Proteins/metabolism ; Plant Viruses/pathogenicity ; Plant Viruses/physiology ; Protein Binding ; Nicotiana/virology ; Vacuolar Proton-Translocating ATPases/metabolism ; Vacuoles/metabolism ; Vacuoles/virology ; Viral Replicase Complex Proteins/chemistry ; Viral Replicase Complex Proteins/metabolism ; Virus Replication
    Chemical Substances Plant Proteins ; Viral Replicase Complex Proteins ; Vacuolar Proton-Translocating ATPases (EC 3.6.1.-)
    Language English
    Publishing date 2021-12-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 586044-1
    ISSN 1460-2075 ; 0261-4189
    ISSN (online) 1460-2075
    ISSN 0261-4189
    DOI 10.15252/embj.2021108713
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: [Advances in genetic engineering of plant virus resistance].

    Haxim, Yakupjan / Ismayil, Asigul / Wang, Yunjing / Liu, Yule

    Sheng wu gong cheng xue bao = Chinese journal of biotechnology

    2015  Volume 31, Issue 6, Page(s) 976–994

    Abstract: Plant virus is one of the most economical devastating microorganisms for global agriculture. Although several strategies are useful for controlling viral infection, such as resistant breeds cultivation, chemical bactericides treatment, blocking the ... ...

    Abstract Plant virus is one of the most economical devastating microorganisms for global agriculture. Although several strategies are useful for controlling viral infection, such as resistant breeds cultivation, chemical bactericides treatment, blocking the infection source, tissue detoxification and field sanitation, viral disease is still a problem in agricultural production. Genetic engineering approach offers various options for introducing virus resistance into crop plants. This paper reviews the current strategies of developing virus resistant transgenic plants.
    MeSH term(s) Agriculture ; Crops, Agricultural/genetics ; Crops, Agricultural/virology ; Genetic Engineering ; Plant Diseases/prevention & control ; Plant Diseases/virology ; Plant Viruses ; Plants, Genetically Modified/virology
    Language Chinese
    Publishing date 2015-06
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1042206-7
    ISSN 1000-3061 ; 1042-749X
    ISSN 1000-3061 ; 1042-749X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants.

    Wang, Yunjing / Gong, Qian / Wu, Yuyao / Huang, Fan / Ismayil, Asigul / Zhang, Danfeng / Li, Huangai / Gu, Hanqing / Ludman, Márta / Fátyol, Károly / Qi, Yijun / Yoshioka, Keiko / Hanley-Bowdoin, Linda / Hong, Yiguo / Liu, Yule

    Cell host & microbe

    2021  Volume 29, Issue 9, Page(s) 1393–1406.e7

    Abstract: RNA interference (RNAi) is an across-kingdom gene regulatory and defense mechanism. However, little is known about how organisms sense initial cues to mobilize RNAi. Here, we show that wounding to Nicotiana benthamiana cells during virus intrusion ... ...

    Abstract RNA interference (RNAi) is an across-kingdom gene regulatory and defense mechanism. However, little is known about how organisms sense initial cues to mobilize RNAi. Here, we show that wounding to Nicotiana benthamiana cells during virus intrusion activates RNAi-related gene expression through calcium signaling. A rapid wound-induced elevation in calcium fluxes triggers calmodulin-dependent activation of calmodulin-binding transcription activator-3 (CAMTA3), which activates RNA-dependent RNA polymerase-6 and Bifunctional nuclease-2 (BN2) transcription. BN2 stabilizes mRNAs encoding key components of RNAi machinery, notably AGONAUTE1/2 and DICER-LIKE1, by degrading their cognate microRNAs. Consequently, multiple RNAi genes are primed for combating virus invasion. Calmodulin-, CAMTA3-, or BN2-knockdown/knockout plants show increased susceptibility to geminivirus, cucumovirus, and potyvirus. Notably, Geminivirus V2 protein can disrupt the calmodulin-CAMTA3 interaction to counteract RNAi defense. These findings link Ca
    MeSH term(s) Argonaute Proteins/genetics ; Argonaute Proteins/metabolism ; Calcium/metabolism ; Calcium Signaling/genetics ; Calmodulin/metabolism ; Cucumovirus/pathogenicity ; Endonucleases/metabolism ; Geminiviridae/pathogenicity ; MicroRNAs/metabolism ; Plant Diseases/prevention & control ; Plant Diseases/virology ; Plants ; Potyviridae/pathogenicity ; RNA Interference/physiology ; RNA, Small Interfering/genetics ; RNA-Dependent RNA Polymerase/metabolism ; Ribonuclease III/genetics ; Ribonuclease III/metabolism ; Nicotiana/genetics ; Nicotiana/virology ; Transcription Factors/metabolism
    Chemical Substances Argonaute Proteins ; Calmodulin ; MicroRNAs ; RNA, Small Interfering ; Transcription Factors ; RNA-Dependent RNA Polymerase (EC 2.7.7.48) ; Endonucleases (EC 3.1.-) ; Ribonuclease III (EC 3.1.26.3) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-08-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2278004-X
    ISSN 1934-6069 ; 1931-3128
    ISSN (online) 1934-6069
    ISSN 1931-3128
    DOI 10.1016/j.chom.2021.07.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Cotton leaf curl Multan virus

    Ismayil, Asigul / Yang, Meng / Haxim, Yakupjan / Wang, Yunjing / Li, Jinlin / Han, Lu / Wang, Yan / Zheng, Xiyin / Wei, Xiang / Nagalakshmi, Ugrappa / Hong, Yiguo / Hanley-Bowdoin, Linda / Liu, Yule

    The Plant cell

    2020  Volume 32, Issue 4, Page(s) 1124–1135

    Abstract: Autophagy plays an important role in plant-pathogen interactions. Several pathogens including viruses induce autophagy in plants, but the underpinning mechanism remains largely unclear. Furthermore, in virus-plant interactions, viral factor(s) that ... ...

    Abstract Autophagy plays an important role in plant-pathogen interactions. Several pathogens including viruses induce autophagy in plants, but the underpinning mechanism remains largely unclear. Furthermore, in virus-plant interactions, viral factor(s) that induce autophagy have yet to be identified. Here, we report that the βC1 protein of
    MeSH term(s) Autophagy ; Autophagy-Related Proteins/metabolism ; Begomovirus/metabolism ; Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism ; Plant Proteins/metabolism ; Protein Binding ; Nicotiana/metabolism ; Nicotiana/ultrastructure ; Nicotiana/virology ; Vacuoles/metabolism ; Vacuoles/ultrastructure ; Viral Proteins/metabolism
    Chemical Substances Autophagy-Related Proteins ; Plant Proteins ; Viral Proteins ; Glyceraldehyde-3-Phosphate Dehydrogenases (EC 1.2.1.-)
    Language English
    Publishing date 2020-02-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 623171-8
    ISSN 1532-298X ; 1040-4651
    ISSN (online) 1532-298X
    ISSN 1040-4651
    DOI 10.1105/tpc.19.00759
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Geminiviral V2 Protein Suppresses Transcriptional Gene Silencing through Interaction with AGO4.

    Wang, Yunjing / Wu, Yuyao / Gong, Qian / Ismayil, Asigul / Yuan, Yuxiang / Lian, Bi / Jia, Qi / Han, Meng / Deng, Haiteng / Hong, Yiguo / Hanley-Bowdoin, Linda / Qi, Yijun / Liu, Yule

    Journal of virology

    2019  Volume 93, Issue 6

    Abstract: In plants, RNA-directed DNA methylation (RdDM)-mediated transcriptional gene silencing (TGS) is a natural antiviral defense against geminiviruses. Several geminiviral proteins have been shown to target the enzymes related to the methyl cycle or histone ... ...

    Abstract In plants, RNA-directed DNA methylation (RdDM)-mediated transcriptional gene silencing (TGS) is a natural antiviral defense against geminiviruses. Several geminiviral proteins have been shown to target the enzymes related to the methyl cycle or histone modification; however, it remains largely unknown whether and by which mechanism geminiviruses directly inhibit RdDM-mediated TGS. In this study, we showed that
    MeSH term(s) Begomovirus/genetics ; DNA Methylation/genetics ; DNA, Viral/genetics ; Geminiviridae/genetics ; Gene Silencing/physiology ; Host-Pathogen Interactions/genetics ; Plant Diseases/virology ; Nicotiana/virology ; Transcription, Genetic/genetics ; Viral Proteins/genetics
    Chemical Substances DNA, Viral ; Viral Proteins
    Language English
    Publishing date 2019-03-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.01675-18
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase.

    Ismayil, Asigul / Haxim, Yakupjan / Wang, Yunjing / Li, Huangai / Qian, Lichao / Han, Ting / Chen, Tianyuan / Jia, Qi / Yihao Liu, Alexander / Zhu, Songbiao / Deng, Haiteng / Gorovits, Rena / Hong, Yiguo / Hanley-Bowdoin, Linda / Liu, Yule

    PLoS pathogens

    2018  Volume 14, Issue 8, Page(s) e1007282

    Abstract: Gene silencing is a natural antiviral defense mechanism in plants. For effective infection, plant viruses encode viral silencing suppressors to counter this plant antiviral response. The geminivirus-encoded C4 protein has been identified as a gene ... ...

    Abstract Gene silencing is a natural antiviral defense mechanism in plants. For effective infection, plant viruses encode viral silencing suppressors to counter this plant antiviral response. The geminivirus-encoded C4 protein has been identified as a gene silencing suppressor, but the underlying mechanism of action has not been characterized. Here, we report that Cotton Leaf Curl Multan virus (CLCuMuV) C4 protein interacts with S-adenosyl methionine synthetase (SAMS), a core enzyme in the methyl cycle, and inhibits SAMS enzymatic activity. By contrast, an R13A mutation in C4 abolished its capacity to interact with SAMS and to suppress SAMS enzymatic activity. Overexpression of wild-type C4, but not mutant C4R13A, suppresses both transcriptional gene silencing (TGS) and post-transcriptional gene silencing (PTGS). Plants infected with CLCuMuV carrying C4R13A show decreased levels of symptoms and viral DNA accumulation associated with enhanced viral DNA methylation. Furthermore, silencing of NbSAMS2 reduces both TGS and PTGS, but enhanced plant susceptibility to two geminiviruses CLCuMuV and Tomato yellow leaf curl China virus. These data suggest that CLCuMuV C4 suppresses both TGS and PTGS by inhibiting SAMS activity to enhance CLCuMuV infection in plants.
    MeSH term(s) Begomovirus/metabolism ; Begomovirus/pathogenicity ; Down-Regulation/genetics ; Gene Expression Regulation, Plant ; Gene Silencing ; Host-Pathogen Interactions/genetics ; Methionine Adenosyltransferase/genetics ; Methionine Adenosyltransferase/metabolism ; Plants, Genetically Modified ; Protein Binding ; RNA Interference ; Nicotiana/genetics ; Nicotiana/metabolism ; Transcription, Genetic ; Viral Proteins/metabolism ; Viral Proteins/physiology
    Chemical Substances Viral Proteins ; Methionine Adenosyltransferase (EC 2.5.1.6)
    Language English
    Publishing date 2018-08-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1007282
    Database MEDical Literature Analysis and Retrieval System OnLINE

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