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  1. Article ; Online: Effect of collagen denaturation degree on mechanical properties and biological activity of nanofibrous scaffolds.

    Tenchurin, Timur Kh / Sytina, Elena V / Solovieva, Elena V / Shepelev, Aleksey D / Mamagulashvili, Vissarion G / Krasheninnikov, Sergey V / Yastremskiy, Evgeniy V / Nesterenko, Elizaveta V / Buzin, Aleksandr I / Istranova, Elena V / Istranov, Leonid P / Fatkhudinov, Timur Kh / Panteleyev, Andrey A / Chvalun, Sergey N

    Journal of biomedical materials research. Part A

    2023  Volume 112, Issue 2, Page(s) 144–154

    Abstract: Further progress in regenerative medicine and bioengineering highly depends on the development of 3D polymeric scaffolds with active biological properties. The most attention is paid to natural extracellular matrix components, primarily collagen. Herein, ...

    Abstract Further progress in regenerative medicine and bioengineering highly depends on the development of 3D polymeric scaffolds with active biological properties. The most attention is paid to natural extracellular matrix components, primarily collagen. Herein, nonwoven nanofiber materials with various degrees of collagen denaturation and fiber diameters 250-500 nm were produced by electrospinning, stabilized by genipin, and characterized in detail. Collagen denaturation has been confirmed using DSC and FTIR analysis. The comparative study of collagen and gelatin nonwoven materials (NWM) revealed only minor differences in their biocompatibility with skin fibroblasts and keratinocytes in vitro. In long-term subcutaneous implantation study, the inflammation was less evident on collagen than on gelatin NWM. Remarkably, the pronounced calcification was revealed in the collagen NWM only. The results obtained can be useful in terms of improving the electrospinning technology of collagen from aqueous solutions, as well as emphasize the importance of long-term study to ensure proper implementation of the material, taking into account the ability of collagen to provoke calcification.
    MeSH term(s) Nanofibers ; Tissue Scaffolds ; Gelatin/pharmacology ; Tissue Engineering/methods ; Collagen/pharmacology
    Chemical Substances Gelatin (9000-70-8) ; Collagen (9007-34-5)
    Language English
    Publishing date 2023-11-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2099989-6
    ISSN 1552-4965 ; 1549-3296 ; 0021-9304
    ISSN (online) 1552-4965
    ISSN 1549-3296 ; 0021-9304
    DOI 10.1002/jbm.a.37598
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6195: Show issues Location:
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  2. Article ; Online: Implantation of Various Cell-Free Matrixes Does Not Contribute to the Restoration of Hyaline Cartilage within Full-Thickness Focal Defects.

    Ibragimova, Shabnam I / Medvedeva, Ekaterina V / Romanova, Irina A / Istranov, Leonid P / Istranova, Elena V / Lychagin, Aleksey V / Nedorubov, Andrey A / Timashev, Peter S / Telpukhov, Vladimir I / Chagin, Andrei S

    International journal of molecular sciences

    2021  Volume 23, Issue 1

    Abstract: Articular cartilage is a highly organized tissue that has a limited ability to heal. Tissue engineering is actively exploited for joint tissue reconstruction in numerous cases of articular cartilage degeneration associated with trauma, arthrosis, ... ...

    Abstract Articular cartilage is a highly organized tissue that has a limited ability to heal. Tissue engineering is actively exploited for joint tissue reconstruction in numerous cases of articular cartilage degeneration associated with trauma, arthrosis, rheumatoid arthritis, and osteoarthritis. However, the optimal scaffolds for cartilage repair are not yet identified. Here we have directly compared five various scaffolds, namely collagen-I membrane, collagen-II membrane, decellularized cartilage, a cellulose-based implant, and commercially available Chondro-Gide
    MeSH term(s) Animals ; Fibrillar Collagens/metabolism ; Hyaline Cartilage/pathology ; Knee Joint/pathology ; Male ; Osteogenesis ; Prosthesis Implantation ; Rats, Wistar ; SOX9 Transcription Factor/metabolism ; Tissue Scaffolds/chemistry ; Rats
    Chemical Substances Fibrillar Collagens ; SOX9 Transcription Factor
    Language English
    Publishing date 2021-12-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23010292
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Mammalian Pericardium-Based Bioprosthetic Materials in Xenotransplantation and Tissue Engineering.

    Grebenik, Ekaterina A / Gafarova, Elvira R / Istranov, Leonid P / Istranova, Elena V / Ma, Xiaowei / Xu, Jing / Guo, Weisheng / Atala, Anthony / Timashev, Peter S

    Biotechnology journal

    2020  Volume 15, Issue 8, Page(s) e1900334

    Abstract: Bioprosthetic materials based on mammalian pericardium tissue are the gold standard in reconstructive surgery. Their application range covers repair of rectovaginal septum defects, abdominoplastics, urethroplasty, duraplastics, maxillofacial, ophthalmic, ...

    Abstract Bioprosthetic materials based on mammalian pericardium tissue are the gold standard in reconstructive surgery. Their application range covers repair of rectovaginal septum defects, abdominoplastics, urethroplasty, duraplastics, maxillofacial, ophthalmic, thoracic and cardiovascular reconstruction, etc. However, a number of factors contribute to the success of their integration into the host tissue including structural organization, mechanical strength, biocompatibility, immunogenicity, surface chemistry, and biodegradability. In order to improve the material's properties, various strategies are developed, such as decellularization, crosslinking, and detoxification. In this review, the existing issues and long-term achievements in the development of bioprosthetic materials based on the mammalian pericardium tissue, aimed at a wide-spectrum application in reconstructive surgery are analyzed. The basic technical approaches to preparation of biocompatible forms providing continuous functioning, optimization of biomechanical and functional properties, and clinical applicability are described.
    MeSH term(s) Animals ; Biocompatible Materials ; Humans ; Mammals ; Pericardium ; Prostheses and Implants/standards ; Tissue Engineering ; Transplantation, Heterologous
    Chemical Substances Biocompatible Materials
    Language English
    Publishing date 2020-03-04
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2221885-3
    ISSN 1860-7314 ; 1860-6768
    ISSN (online) 1860-7314
    ISSN 1860-6768
    DOI 10.1002/biot.201900334
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    Zs.A 6349: Show issues Location:
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  4. Article ; Online: Chemical cross-linking of xenopericardial biomeshes: A bottom-up study of structural and functional correlations.

    Grebenik, Ekaterina A / Istranov, Leonid P / Istranova, Elena V / Churbanov, Semyon N / Shavkuta, Boris S / Dmitriev, Ruslan I / Veryasova, Nadezhda N / Kotova, Svetlana L / Kurkov, Alexander V / Shekhter, Anatoly B / Timashev, Peter S

    Xenotransplantation

    2019  Volume 26, Issue 3, Page(s) e12506

    Abstract: Decellularized bovine pericardium (DBP)-based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after the transplantation, various chemical cross-linking strategies are employed. However, structural and ... ...

    Abstract Decellularized bovine pericardium (DBP)-based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after the transplantation, various chemical cross-linking strategies are employed. However, structural and functional properties of the biomeshes differ in dependence on the cross-linker used. Here, we performed a bottom-up study of structural and functional alterations of DBP-based biomeshes following cross-linking with hexamethylene diisocyanate (HMDC), ethylene glycol diglycidyl ether (EGDE), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and genipin. The in vitro cytotoxicity tests supported their clinical applicability. Their structural differences (eg roughness, fibre thickness, pore morphology) were evaluated using the two-photon confocal laser scanning, atomic force, scanning electron and polarized light microscopies. HMDC and EDC samples appeared to be the roughest. Complex mechanical trials indicated the tendency to reduced Young's Modulus and mechanical anisotropy values of DBP upon cross-linking. The lowest mechanical anisotropy was found in EDC and genipin sample groups. In vitro collagenase susceptibility was the highest for EDC samples and the lowest for EGDE samples. The comparative analysis of the results allowed us to recognize the strengths and weaknesses of each cross-linker in relation to a particular clinical application.
    MeSH term(s) Animals ; Cattle ; Cross-Linking Reagents ; Iridoids/pharmacology ; Materials Testing/methods ; Pericardium/surgery ; Tissue Engineering/methods ; Transplantation, Heterologous
    Chemical Substances Cross-Linking Reagents ; Iridoids ; genipin (A3V2NE52YG)
    Language English
    Publishing date 2019-01-22
    Publishing country Denmark
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1236298-0
    ISSN 1399-3089 ; 0908-665X
    ISSN (online) 1399-3089
    ISSN 0908-665X
    DOI 10.1111/xen.12506
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    Zs.A 4514: Show issues Location:
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  5. Article ; Online: Control on rheological behavior of collagen 1 dispersions for efficient electrospinning.

    Tenchurin, Timur Kh / Belousov, Sergey I / Kiryukhin, Yury I / Istranov, Leonid P / Istranova, Elena V / Shepelev, Alexey D / Mamagulashvili, Vissarion G / Kamyshinsky, Roman A / Chvalun, Sergei N

    Journal of biomedical materials research. Part A

    2018  Volume 107, Issue 2, Page(s) 312–318

    Abstract: For efficient manufacturing of fibrous collagen-based materials by electrospinning, the search on optimal rheological parameters is of the great importance. Rheological characteristics and denaturation of collagen in aqueous dispersions were studied as a ...

    Abstract For efficient manufacturing of fibrous collagen-based materials by electrospinning, the search on optimal rheological parameters is of the great importance. Rheological characteristics and denaturation of collagen in aqueous dispersions were studied as a function of shear rate and acetic acid concentration in the range of 3-9% w/w at temperature from 20 to 40°C. It was shown that an increase in temperature, acetic acid concentration of the collagen dispersion leads to a significant decrease in its viscosity. It was found that helical conformation of the collagen macromolecules is preserved up to 31°C. An increase in acetic acid concentration leads to a reduction of denaturation temperature. The complex viscosity of collagen dispersions exhibits a sharp drop, followed by a rapid growth of damping factor in the temperature range from 22 to 35°C. Both storage (G') and loss (G″) moduli increase with frequency and collagen concentration. It was revealed that optimal parameters for electrospinning of highly concentrated collagen dispersions can be achieved by adjusting of the concentration of acetic acid, temperature, and stirring speed. As a result, collagen nonwoven materials with diameter from 100 to 700 nm were obtained. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 312-318, 2019.
    MeSH term(s) Animals ; Cattle ; Collagen Type I/chemistry ; Collagen Type I/ultrastructure ; Nanofibers/chemistry ; Nanofibers/ultrastructure ; Protein Conformation, alpha-Helical ; Protein Denaturation ; Rheology ; Temperature ; Viscosity
    Chemical Substances Collagen Type I
    Language English
    Publishing date 2018-11-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2099989-6
    ISSN 1552-4965 ; 1549-3296 ; 0021-9304
    ISSN (online) 1552-4965
    ISSN 1549-3296 ; 0021-9304
    DOI 10.1002/jbm.a.36459
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6195: Show issues Location:
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  6. Article ; Online: Dinitrosyl iron complexes with glutathione incorporated into a collagen matrix as a base for the design of drugs accelerating skin wound healing.

    Shekhter, Anatoly B / Rudenko, Tatyana G / Istranov, Leonid P / Guller, Anna E / Borodulin, Rostislav R / Vanin, Anatoly F

    European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

    2015  Volume 78, Page(s) 8–18

    Abstract: Composites of a collagen matrix and dinitrosyl iron complexes with glutathione (DNIC-GS) (in a dose of 4.0 μmoles per item) in the form of spongy sheets (DNIC-Col) were prepared and then topically applied in rat excisional full-thickness skin wound model. ...

    Abstract Composites of a collagen matrix and dinitrosyl iron complexes with glutathione (DNIC-GS) (in a dose of 4.0 μmoles per item) in the form of spongy sheets (DNIC-Col) were prepared and then topically applied in rat excisional full-thickness skin wound model. The effects of DNIC-Col were studied in comparison with spontaneously healing wounds (SpWH) and wounds treated with collagen sponges (Col) without DNIC-GS. The composites induced statistically and clinically significant acceleration of complete wound closure (21±1 day versus 23±1 day and 26±1 day for DNIC-Col, Col and SpWH, respectively). Histological examination of wound tissues on days 4, 14, 18 and 21 after surgery demonstrated that this improvement was supported by enhanced growth, maturation and fibrous transformation of granulation tissue and earlier epithelization of the injured area in rats treated with DNIC-Col composites benchmarked against Col and SpWH. It is suggested that the positive effect of the new pharmaceutical material on wound healing is based on the release of NO from decomposing DNIC. This effect is believed to be potentiated by the synergy of DNIC and collagen.
    MeSH term(s) Animals ; Collagen/administration & dosage ; Collagen/chemistry ; Collagen/therapeutic use ; Drug Delivery Systems ; Glutathione/administration & dosage ; Glutathione/chemistry ; Glutathione/therapeutic use ; Iron/administration & dosage ; Iron/chemistry ; Iron/therapeutic use ; Male ; Nitric Oxide Donors/administration & dosage ; Nitric Oxide Donors/chemistry ; Nitric Oxide Donors/therapeutic use ; Nitrogen Oxides/administration & dosage ; Nitrogen Oxides/chemistry ; Nitrogen Oxides/therapeutic use ; Rats ; Skin/injuries ; Skin/pathology ; Wound Healing/drug effects
    Chemical Substances Nitric Oxide Donors ; Nitrogen Oxides ; dinitrosyl iron complex (68586-27-6) ; Collagen (9007-34-5) ; Iron (E1UOL152H7) ; Glutathione (GAN16C9B8O)
    Language English
    Publishing date 2015-10-12
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1154366-8
    ISSN 1879-0720 ; 0928-0987
    ISSN (online) 1879-0720
    ISSN 0928-0987
    DOI 10.1016/j.ejps.2015.06.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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