Article: Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus.
2024 Volume 12, Issue 2
Abstract: In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets ... ...
| Abstract | In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating that its antigenic properties were retained. Two groups of Balb/c mice were immunized with intramuscular injection of recombinant hemagglutinin or propiolactone inactivated A/Astrakhan/3212/2020 (H5N8) influenza virus. The results demonstrated that both immunogens induced a specific antibody response as determined by ELISA. Virus neutralization assay revealed that sera of immunized animals were able to neutralize A/turkey/Stavropol/320-01/2020 (H5N8) influenza virus-the average neutralizing titer was 2560. Immunization with both recombinant HA/H5 hemagglutinin and inactivated virus gave 100% protection against lethal H5N8 virus challenge. This study shows that recombinant HA (H5N8) protein may be a useful antigen candidate for developing subunit vaccines against influenza A (H5N8) virus with suitable immunogenicity and protective efficacy. |
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| Language | English |
| Publishing date | 2024-01-29 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2703319-3 |
| ISSN | 2076-393X |
| ISSN | 2076-393X |
| DOI | 10.3390/vaccines12020143 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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