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Article: CSF spectrophotometry in the diagnosis of subarachnoid haemorrhage.

Iversen, S A

2001 Volume 55, Issue 8, Page(s) 640

MeSH term(s)	False Positive Reactions ; Humans ; Iodine ; Specimen Handling ; Spectrophotometry ; Spinal Puncture ; Subarachnoid Hemorrhage/cerebrospinal fluid ; Subarachnoid Hemorrhage/diagnosis
Chemical Substances	Iodine (9679TC07X4)
Language	English
Publishing date	2001-02-06
Publishing country	England
Document type	Comment ; Letter
ZDB-ID	80261-x
ISSN	1472-4146 ; 0021-9746
ISSN (online)	1472-4146
ISSN	0021-9746
DOI	10.1136/jcp.55.8.640
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Article: Simultaneous quantitation of homocysteine, cysteine and methionine in plasma and urine by liquid chromatography-tandem mass spectrometry.

Weaving, G / Rocks, B F / Iversen, S A / Titheradge, M A

Annals of clinical biochemistry

2006 Volume 43, Issue Pt 6, Page(s) 474–480

Abstract: Background: A method utilizing liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) has been developed and evaluated for the determination of total homocysteine, cysteine and methionine in plasma and urine. The simultaneous measurement ...

Abstract	Background: A method utilizing liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) has been developed and evaluated for the determination of total homocysteine, cysteine and methionine in plasma and urine. The simultaneous measurement of homocysteine and methionine concentrations may help explain the underlying mechanism responsible for hyperhomo-cysteinaemia. Methods: Samples were prepared by simple protein precipitation after reduction of disulphides by dithiothreitol. Reduced analyte signal caused by ionization suppression effects, seen with plasma samples, was compensated for with matrix-matched standards, and the use of isotopically labelled internal standards. Recovery for each analyte was better than 94%. Results: Concentrations of plasma homocysteine determined by LC-MS/MS were compared with those obtained by two automated commercially available FDA-approved procedures: (i) high-performance liquid chromatography (HPLC) with pre-column derivatization and fluorescence detection and (ii) by fluorescence polarization immunoassay (FPIA). Agreement with the LC-MS/MS method is given by the Deming regression equations LC-MS/MS = 1.062 HPLC-0.01 and LC-MS/MS = 1.104 FPIA-0.43. Conclusion: Low reagent costs together with the relative simplicity of sample preparation make the LC-MS/MS method well suited, not only for research work but also in those laboratories with a tandem mass spectrometer, for the measurement of routine clinical samples.
MeSH term(s)	Chromatography, High Pressure Liquid/methods ; Cysteine/blood ; Cysteine/urine ; Cystine/blood ; Homocysteine/blood ; Homocysteine/urine ; Humans ; Methionine/blood ; Methionine/urine ; Reproducibility of Results ; Tandem Mass Spectrometry/methods
Chemical Substances	Homocysteine (0LVT1QZ0BA) ; Cystine (48TCX9A1VT) ; Methionine (AE28F7PNPL) ; Cysteine (K848JZ4886)
Language	English
Publishing date	2006-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390309-6
ISSN	1758-1001 ; 0004-5632
ISSN (online)	1758-1001
ISSN	0004-5632
DOI	10.1258/000456306778904605
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Article: Behavioural and psychological symptoms of Alzheimer type dementia are not correlated with plasma homocysteine concentration.

Tabet, N / Rafi, H / Weaving, G / Lyons, B / Iversen, S A

Dementia and geriatric cognitive disorders

2006 Volume 22, Issue 5-6, Page(s) 432–438

Abstract: Background/aims: Vitamin B12 and folate deficiencies have been associated with cognitive impairment and various psychiatric symptoms but not specifically with behavioural and psychological symptoms of dementia (BPSD). A limitation of previous studies in ...

Abstract	Background/aims: Vitamin B12 and folate deficiencies have been associated with cognitive impairment and various psychiatric symptoms but not specifically with behavioural and psychological symptoms of dementia (BPSD). A limitation of previous studies in dementia was lack of concurrent homocysteine measurement especially as it may provide a better indicator of tissue activities of these vitamins. This study was designed to clarify whether a relationship exists between plasma homocysteine concentration and BPSD. Methods: Plasma homocysteine, serum vitamin B12 and folate were measured in 23 Alzheimer's disease (AD) patients with BPSD and 27 AD patients without BPSD as determined through the use of the Neuropsychiatric Inventory (NPI). Blood levels of measured substances were also correlated with individual NPI scores and with cumulative NPI scores for different cluster of symptoms. Results: There was no significant difference (p = 0.956) in the mean plasma homocysteine levels between AD patients with BPSD (17.48 micromol/l) and AD patients without BPSD (17.34 micromol/l). Similarly, there was no significant difference between the two groups in the mean serum B12 (382.61 and 391.60 pg/ml, respectively) and folate (7.95 and 10.02 ng/ml, respectively). Mean levels for both vitamins were well within the laboratory reference range. Neither individual nor cluster NPI scores correlated significantly with plasma homocysteine. Conclusion: This study shows for the first time that BPSD are not associated with hyperhomocysteinaemia in Alzheimer dementia. Although previous studies have identified homocysteine as an independent risk factor in AD, the results reported here do not lend weight to an aetiological role for homocysteine specifically in BPSD.
MeSH term(s)	Aged ; Aged, 80 and over ; Alzheimer Disease/blood ; Alzheimer Disease/diagnosis ; Alzheimer Disease/psychology ; Female ; Folic Acid/blood ; Homocysteine/blood ; Humans ; Male ; Mental Disorders/diagnosis ; Mental Disorders/etiology ; Mental Disorders/psychology ; Neuropsychological Tests ; Psychological Tests ; Severity of Illness Index ; Vitamin B 12/blood
Chemical Substances	Homocysteine (0LVT1QZ0BA) ; Folic Acid (935E97BOY8) ; Vitamin B 12 (P6YC3EG204)
Language	English
Publishing date	2006
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1026007-9
ISSN	1420-8008 ; 1013-7424
ISSN	1420-8008 ; 1013-7424
DOI	10.1159/000095802
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Zs.A 2866: Show issues

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Article: Limitation of the Vitros dry-slide technique in measuring high concentrations of salicylate in plasma.

Iversen, S A / Walker, A / Avades, T Y / Wilson, J F

Annals of clinical biochemistry

2001 Volume 38, Issue Pt 6, Page(s) 711–713

Abstract: The linearity of the Vitros dry-slide method for plasma salicylate was assessed in two ways: serial concentrations of sodium salicylate were added to fresh lithium heparin plasma, and the salicylate was determined both neat and in dilution. Vitros ... ...

Abstract	The linearity of the Vitros dry-slide method for plasma salicylate was assessed in two ways: serial concentrations of sodium salicylate were added to fresh lithium heparin plasma, and the salicylate was determined both neat and in dilution. Vitros salicylate results submitted to the Heathcontrol External Quality Assessment Scheme were compared to the spike value. Similar loss of linearity was observed in both cases. Serious salicylate overdosage requiring specific clinical treatment may have been underestimated.
MeSH term(s)	Blood Chemical Analysis/instrumentation ; Blood Chemical Analysis/methods ; Blood Chemical Analysis/standards ; Blood Chemical Analysis/statistics & numerical data ; Drug Overdose/blood ; Drug Overdose/diagnosis ; False Negative Reactions ; Humans ; Quality Control ; Reference Standards ; Salicylic Acid/blood ; Salicylic Acid/poisoning ; Salicylic Acid/standards
Chemical Substances	Salicylic Acid (O414PZ4LPZ)
Language	English
Publishing date	2001-11
Publishing country	England
Document type	Journal Article
ZDB-ID	390309-6
ISSN	1758-1001 ; 0004-5632
ISSN (online)	1758-1001
ISSN	0004-5632
DOI	10.1258/0004563011900948
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Article: Does routine blood bone biochemistry predict vitamin D insufficiency in elderly patients with low-velocity fractures?

Singh, S K / Manjure, S / Stott, P / Shetty, A / Iversen, S A / Williams, C R

Journal of orthopaedic surgery (Hong Kong)

2004 Volume 12, Issue 1, Page(s) 31–34

Abstract: Purpose: Vitamin D deficiency impairs bone mineralisation and can predispose individuals to fractures. This study aimed at testing whether measurement of plasma calcium, alkaline phosphatase, and phosphate levels could detect vitamin D insufficiency.: ...

Abstract	Purpose: Vitamin D deficiency impairs bone mineralisation and can predispose individuals to fractures. This study aimed at testing whether measurement of plasma calcium, alkaline phosphatase, and phosphate levels could detect vitamin D insufficiency. Methods: During a 10-week winter period from December 2000 to February 2001, all elderly patients presenting to a general hospital in Brighton--British seaside town--with a fracture of the proximal femur and without known bone mineralisation problems were invited to participate in the study. Results: 23 (63.9%) of the 36 eligible patients had insufficient levels of vitamin D, with a plasma concentration of less than 30 nmol/L. The mean parathyroid hormone level was 56 pg/mL (range, 12-193 pg/mL). 11 of the 36 patients had an elevated level of parathyroid hormone were insufficient in vitamin D. The mean plasma concentration of calcium was 2.30 mmol/L (range, 2.05-2.98 mmol/L). The mean phosphate level was 0.98 mmol/L (range, 0.40-1.79 mmol/L), and the mean alkaline phosphatase level was 91 IU/L (range, 46-127 IU/L). There was poor correlation between vitamin D insufficiency and plasma calcium, alkaline phosphatase, or phosphate levels. Conclusion: Plasma calcium, alkaline phosphatase, and phosphate testing cannot detect vitamin D insufficiency. We recommend that vitamin D and calcium supplementation be considered for patients with low-energy hip fractures.
MeSH term(s)	Accidental Falls ; Age Factors ; Aged ; Aged, 80 and over ; Blood Chemical Analysis ; Bone Density/physiology ; Cohort Studies ; Diagnostic Tests, Routine ; Female ; Geriatric Assessment ; Hip Fractures/blood ; Hip Fractures/epidemiology ; Hip Fractures/therapy ; Humans ; Incidence ; Injury Severity Score ; Male ; Osteoporosis/complications ; Osteoporosis/diagnosis ; Predictive Value of Tests ; Primary Prevention/methods ; Risk Assessment ; Sex Factors ; United Kingdom/epidemiology ; Vitamin D Deficiency/complications ; Vitamin D Deficiency/diagnosis
Language	English
Publishing date	2004-06
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	1493368-8
ISSN	1022-5536
ISSN	1022-5536
DOI	10.1177/230949900401200107
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Zs.A 5104: Show issues

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Article: The acceptability of resin therapy to patients attending a hospital lipid clinic.

Iversen, S A / Davidson, D / Davidson, C

British journal of clinical pharmacology

1995 Volume 40, Issue 1, Page(s) 92–94

Abstract: Low dose cholestyramine ('Questran A') and colestipol ('Colestid Orange') were compared in a blinded two period crossover study of 55 patients attending a hospital lipid clinic. Colestipol was rated higher on a combined acceptability/palatability score. ... ...

Abstract	Low dose cholestyramine ('Questran A') and colestipol ('Colestid Orange') were compared in a blinded two period crossover study of 55 patients attending a hospital lipid clinic. Colestipol was rated higher on a combined acceptability/palatability score. Both treatments reduced low density lipoprotein (LDL) cholesterol to a similar extent.
MeSH term(s)	Adolescent ; Adult ; Aged ; Anion Exchange Resins/therapeutic use ; Anticholesteremic Agents/therapeutic use ; Cholesterol, LDL/blood ; Cholestyramine Resin/therapeutic use ; Colestipol/therapeutic use ; Cross-Over Studies ; Female ; Humans ; Hypercholesterolemia/blood ; Hypercholesterolemia/drug therapy ; Hypolipidemic Agents/therapeutic use ; Inpatients ; Male ; Middle Aged ; Surveys and Questionnaires
Chemical Substances	Anion Exchange Resins ; Anticholesteremic Agents ; Cholesterol, LDL ; Hypolipidemic Agents ; Cholestyramine Resin (11041-12-6) ; Colestipol (K50N755924)
Language	English
Publishing date	1995-07
Publishing country	England
Document type	Clinical Trial ; Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	188974-6
ISSN	1365-2125 ; 0306-5251 ; 0264-3774
ISSN (online)	1365-2125
ISSN	0306-5251 ; 0264-3774
DOI	10.1111/j.1365-2125.1995.tb04542.x
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Article ; Online: Intensive care doctors' preferences for arterial oxygen tension levels in mechanically ventilated patients.

Schjørring, O L / Toft-Petersen, A P / Kusk, K H / Mouncey, P / Sørensen, E E / Berezowicz, P / Bestle, M H / Bülow, H-H / Bundgaard, H / Christensen, S / Iversen, S A / Kirkeby-Garstad, I / Krarup, K B / Kruse, M / Laake, J H / Liboriussen, L / Laebel, R L / Okkonen, M / Poulsen, L M /

Russell, L / Sjövall, F / Sunde, K / Søreide, E / Waldau, T / Walli, A R / Perner, A / Wetterslev, J / Rasmussen, B S

Acta anaesthesiologica Scandinavica

2018 Volume 62, Issue 10, Page(s) 1443–1451

Abstract: Background: Oxygen is liberally administered in intensive care units (ICUs). Nevertheless, ICU doctors' preferences for supplementing oxygen are inadequately described. The aim was to identify ICU doctors' preferences for arterial oxygenation levels in ... ...

Abstract	Background: Oxygen is liberally administered in intensive care units (ICUs). Nevertheless, ICU doctors' preferences for supplementing oxygen are inadequately described. The aim was to identify ICU doctors' preferences for arterial oxygenation levels in mechanically ventilated adult ICU patients. Methods: In April to August 2016, an online multiple-choice 17-part-questionnaire was distributed to 1080 ICU doctors in seven Northern European countries. Repeated reminder e-mails were sent. The study ended in October 2016. Results: The response rate was 63%. When evaluating oxygenation 52% of respondents rated arterial oxygen tension (PaO Conclusion: Intensive care unit doctors preferred PaO
MeSH term(s)	Humans ; Intensive Care Units ; Oxygen/blood ; Oxygen/toxicity ; Physicians ; Pulmonary Disease, Chronic Obstructive/metabolism ; Respiration, Artificial ; Respiratory Distress Syndrome, Adult/metabolism
Chemical Substances	Oxygen (S88TT14065)
Language	English
Publishing date	2018-06-21
Publishing country	England
Document type	Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
ZDB-ID	80002-8
ISSN	1399-6576 ; 0001-5172
ISSN (online)	1399-6576
ISSN	0001-5172
DOI	10.1111/aas.13171
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Article: Direct measurement of low density lipoprotein in whole blood by silver-enhanced gold-labelled immunoassay.

Patel, N / Rocks, B F / Iversen, S A

Annals of clinical biochemistry

1992 Volume 29 Pt 3, Page(s) 283–286

Abstract: A competitive silver-enhanced gold-labelled immunoassay has been developed for the direct measurement of low density lipoprotein (LDL) in whole blood. Immobilized LDL and sample LDL compete for added antibody. Quantitation of the bound antibody/antigen ... ...

Abstract	A competitive silver-enhanced gold-labelled immunoassay has been developed for the direct measurement of low density lipoprotein (LDL) in whole blood. Immobilized LDL and sample LDL compete for added antibody. Quantitation of the bound antibody/antigen complex is achieved by the addition of gold-labelled anti-immunoglobulin G followed by enhancement of absorbance by addition of silver ions. Whole-blood samples from fasting patients were assayed directly for LDL by the procedure and the corresponding plasma samples were assayed for total cholesterol, high density lipoprotein and triglycerides followed by the indirect calculation of LDL cholesterol. The correlation between the two methods was good (r = 0.82) and the SEGLISA exhibited good precision.
MeSH term(s)	Cardiovascular Diseases/blood ; Gold ; Humans ; Immunoassay/methods ; Lipoproteins, LDL/blood ; Silver/pharmacology
Chemical Substances	Lipoproteins, LDL ; Silver (3M4G523W1G) ; Gold (7440-57-5)
Language	English
Publishing date	1992-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390309-6
ISSN	1758-1001 ; 0004-5632
ISSN (online)	1758-1001
ISSN	0004-5632
DOI	10.1177/000456329202900305
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Article: Direct measurement of low density lipoprotein in whole blood.

Patel, N / Rocks, B F / Iversen, S A

Clinical biochemistry

1992 Volume 25, Issue 5, Page(s) 401–403

MeSH term(s)	Humans ; Immunoassay/methods ; Lipoproteins, LDL/blood
Chemical Substances	Lipoproteins, LDL
Language	English
Publishing date	1992-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	390372-2
ISSN	0009-9120
ISSN	0009-9120
DOI	10.1016/0009-9120(92)80025-c
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Article: Phylogeography and population history of Atlantic mackerel (Scomber scombrus L.): a genealogical approach reveals genetic structuring among the eastern Atlantic stocks.

Nesbø, C L / Rueness, E K / Iversen, S A / Skagen, D W / Jakobsen, K S

Proceedings. Biological sciences

2000 Volume 267, Issue 1440, Page(s) 281–292

Abstract: Despite the resolving power of DNA markers, pelagic and migratory marine fish species generally show very little geographical population structuring. In mackerel (Scomber scombrus L.) population differentiation has been detected only at a transatlantic ... ...

Abstract	Despite the resolving power of DNA markers, pelagic and migratory marine fish species generally show very little geographical population structuring. In mackerel (Scomber scombrus L.) population differentiation has been detected only at a transatlantic scale. By applying two regions in mitochondrial DNA (mtDNA) (D-loop and cytochrome b (cytb)) in combination with genealogical and frequency-based statistical approaches, our data suggest population differentiation among eastern Atlantic spawning stocks. In contrast, and indicative of homing behaviour, no genetic structuring was observed among shoals of individuals outside the spawning season. Among spawning stocks, mtDNA D-loop sequences detected differentiation within the eastern Atlantic, while the cytb gene detected transatlantic differentiation. The impact of recurrent events (e.g. gene flow restricted by isolation by distance) and historic events (e.g. population range expansions) among spawning stocks was investigated applying a nested cladistic analysis of geographical distribution of cytb haplotype lineages. In the eastern Atlantic, historical population range expansion, presumably in connection with recolonization of northern areas after the last glaciation, is suggested to be the main factor determining mtDNA lineage distribution. This was supported by estimates of mtDNA nucleotide diversity, where the highest diversity was observed for the stock spawning in the Bay of Biscay, for which the size estimate is only 15% of the largest stock (Celtic Sea). In addition to revealing population differentiation, our data demonstrate the importance of sampling strategy and the power of applying statistical methods addressing both ongoing and historical population processes.
MeSH term(s)	Animals ; Atlantic Ocean ; Cytochrome b Group/genetics ; DNA, Mitochondrial/genetics ; Genetic Variation ; Haplotypes ; Perciformes/classification ; Perciformes/genetics ; Perciformes/physiology ; Phylogeny ; Sequence Analysis, DNA
Chemical Substances	Cytochrome b Group ; DNA, Mitochondrial
Language	English
Publishing date	2000-02-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209242-6
ISSN	1471-2954 ; 0962-8452 ; 0080-4649 ; 0950-1193
ISSN (online)	1471-2954
ISSN	0962-8452 ; 0080-4649 ; 0950-1193
DOI	10.1098/rspb.2000.0998
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