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  1. Article: Fully Automated Bioreactor-Based pH-Cycling System for Demineralization: A Comparative Study with a Conventional Method.

    Satou, Ryouichi / Miki, Naoko / Iwasaki, Miyu / Sugihara, Naoki

    Materials (Basel, Switzerland)

    2023  Volume 16, Issue 14

    Abstract: This study aimed to develop an automated pH-cycling system using inexpensive commercial components that can replicate pH fluctuations in the oral cavity and salivary clearance to compare demineralization characteristics with the conventional method. The ... ...

    Abstract This study aimed to develop an automated pH-cycling system using inexpensive commercial components that can replicate pH fluctuations in the oral cavity and salivary clearance to compare demineralization characteristics with the conventional method. The study found that the newly developed cycle-1 group showed improved demineralization properties, including apparent lesion depth, surface roughness, Vickers hardness, mineral loss, and depth of demineralization, compared to the control group. Additionally, the cycle-2 group, which had a longer cycle interval, showed further improvements in the demineralization properties. This system can replicate the differences in dental damage caused by differences in meals, snacking frequencies, and lifestyle rhythms, making it useful in cariology, preventive dentistry research, and oral care product development. It can be constructed using inexpensive commercial products, significantly reducing research costs and improving reproducibility and fairness between different experimental facilities. The system can replicate lifestyle rhythms, such as meals, sleep, and oral clearance by saliva, making it an in vitro pseudo-oral cavity.
    Language English
    Publishing date 2023-07-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma16144929
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Development of Root Caries Prevention by Nano-Hydroxyapatite Coating and Improvement of Dentin Acid Resistance.

    Iwasaki, Miyu / Satou, Ryouichi / Sugihara, Naoki

    Materials (Basel, Switzerland)

    2022  Volume 15, Issue 22

    Abstract: There is no established method for optimizing the use of dentin to prevent root caries, which are increasing in the elderly population. This study aimed to develop a new approach for root caries prevention by focusing on bioapatite (BioHap), a new ... ...

    Abstract There is no established method for optimizing the use of dentin to prevent root caries, which are increasing in the elderly population. This study aimed to develop a new approach for root caries prevention by focusing on bioapatite (BioHap), a new biomaterial, combined with fluoride. Bovine dentin was used as a sample, and an acid challenge was performed in three groups: no fluoride (control group), acidulated phosphate fluoride treatment (APF group), and BioHap + APF treatment (BioHap group). After applying the new compound, the acid resistance of dentin was compared with that of APF alone. The BioHap group had fewer defects and an increased surface hardness than the APF group. The BioHap group had the smallest lesion depth and least mineral loss among all groups. Using a scanning electron microscope in the BioHap group showed the closure of dentinal tubules and a coating on the surface. The BioHap group maintained a coating and had higher acid resistance than the APF group. The coating prevents acid penetration, and the small particle size of BioHap and its excellent reactivity with fluoride are thought to have contributed to the improvement of acid resistance in dentin. Topical fluoride application using BioHap protects against root caries.
    Language English
    Publishing date 2022-11-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma15228263
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Improved Enamel Acid Resistance Using Biocompatible Nano-Hydroxyapatite Coating Method.

    Satou, Ryouichi / Iwasaki, Miyu / Kamijo, Hideyuki / Sugihara, Naoki

    Materials (Basel, Switzerland)

    2022  Volume 15, Issue 20

    Abstract: In this study, we attempted to develop a dental caries prevention method using a bioapatite (BioHap), an eggshell-derived apatite with nanoparticle size and biocompatibility, with a high-concentration fluoride tooth surface application method. The enamel ...

    Abstract In this study, we attempted to develop a dental caries prevention method using a bioapatite (BioHap), an eggshell-derived apatite with nanoparticle size and biocompatibility, with a high-concentration fluoride tooth surface application method. The enamel acid resistance after the application of the proposed method was compared with that of a conventional topical application of fluoride using bovine tooth enamel as an example. The tooth samples were divided into three groups based on the preventive treatment applied, and an acid challenge was performed. The samples were evaluated for acid resistance using qualitative and quantitative analytical methods. The BioHap group demonstrated reduced enamel loss and improved micro-Vickers hardness, along with a thick coating layer, decreased reaction area depth, and decreased mineral loss value and lesion depth. The combination of BioHap with high-concentration fluoride led to the formation of a thick coating layer on the enamel surface and better suppression of demineralization than the conventional method, both qualitatively and quantitatively. The proposed biocompatible nano-hydroxyapatite coating method is expected to become a new standard for providing professional care to prevent dental caries.
    Language English
    Publishing date 2022-10-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma15207171
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Improved Enamel Acid Resistance by Highly Concentrated Acidulated Phosphate Sodium Monofluorophosphate Solution.

    Satou, Ryouichi / Yamagishi, Atsushi / Takayanagi, Atsushi / Iwasaki, Miyu / Kamijo, Hideyuki / Sugihara, Naoki

    Materials (Basel, Switzerland)

    2022  Volume 15, Issue 20

    Abstract: Sodium monofluorophosphate (MFP) is a component of fluoride-containing dentifrices and is more biosafe than the conventional sodium fluoride (NaF). MFP can respond not only on the tooth surface layer but also deep into the enamel. We aim to confirm that ... ...

    Abstract Sodium monofluorophosphate (MFP) is a component of fluoride-containing dentifrices and is more biosafe than the conventional sodium fluoride (NaF). MFP can respond not only on the tooth surface layer but also deep into the enamel. We aim to confirm that high concentrations of acid phosphate MFP (AP-MFP, 9000 ppmF), used in professional care, could lead to a highly biosafe fluoride application method that acts through the deep enamel layers. Sample groups were respectively treated in vitro with NaF, acidulated phosphate fluoride (APF), MFP, and AP-MFP, and the samples were compared against an untreated group. Characterizations after fluoride application confirmed that MFP and AP-MFP treatments improved the acid resistance of enamel compared to that of conventional methods. Furthermore, the acid resistance of highly concentrated MFPs improved by using phosphoric acid. Although the acid resistance from the AP-MFP method is not as good as that using APF, AP-MFP can act both on the surface layer and deep into the enamel. Moreover, AP-MFP retains fluoride ions as much as APF does on the tooth surface. The proposed fluoride application method using AP-MFP introduces a dental treatment for acid resistance that is highly biosafe and penetrates deep layers of the enamel.
    Language English
    Publishing date 2022-10-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma15207298
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Plasma Membrane Ca

    Kimura, Maki / Mochizuki, Hiroyuki / Satou, Ryouichi / Iwasaki, Miyu / Kokubu, Eitoyo / Kono, Kyosuke / Nomura, Sachie / Sakurai, Takeshi / Kuroda, Hidetaka / Shibukawa, Yoshiyuki

    Biomolecules

    2021  Volume 11, Issue 7

    Abstract: Intracellular ... ...

    Abstract Intracellular Ca
    Language English
    Publishing date 2021-07-10
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom11071010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Physiologically Based Pharmacokinetic Models Predicting Renal and Hepatic Concentrations of Industrial Chemicals after Virtual Oral Doses in Rats.

    Kamiya, Yusuke / Otsuka, Shohei / Miura, Tomonori / Yoshizawa, Manae / Nakano, Ayane / Iwasaki, Miyu / Kobayashi, Yui / Shimizu, Makiko / Kitajima, Masato / Shono, Fumiaki / Funatsu, Kimito / Yamazaki, Hiroshi

    Chemical research in toxicology

    2020  Volume 33, Issue 7, Page(s) 1736–1751

    Abstract: Recently developed high-throughput in vitro assays in combination with computational models could provide alternatives to animal testing. The purpose of the present study was to model the plasma, hepatic, and renal pharmacokinetics of approximately 150 ... ...

    Abstract Recently developed high-throughput in vitro assays in combination with computational models could provide alternatives to animal testing. The purpose of the present study was to model the plasma, hepatic, and renal pharmacokinetics of approximately 150 structurally varied types of drugs, food components, and industrial chemicals after virtual external oral dosing in rats and to determine the relationship between the simulated internal concentrations in tissue/plasma and their lowest-observed-effect levels. The model parameters were based on rat plasma data from the literature and empirically determined pharmacokinetics measured after oral administrations to rats carried out to evaluate hepatotoxic or nephrotic potentials. To ensure that the analyzed substances exhibited a broad diversity of chemical structures, their structure-based location in the chemical space underwent projection onto a two-dimensional plane, as reported previously, using generative topographic mapping. A high-throughput in silico one-compartment model and a physiologically based pharmacokinetic (PBPK) model consisting of chemical receptor (gut), metabolizing (liver), central (main), and excreting (kidney) compartments were developed in parallel. For 159 disparate chemicals, the maximum plasma concentrations and the areas under the concentration-time curves obtained by one-compartment models and modified simple PBPK models were closely correlated. However, there were differences between the PBPK modeled and empirically obtained hepatic/renal concentrations and plasma maximal concentrations/areas under the concentration-time curves of the 159 chemicals. For a few compounds, the lowest-observed-effect levels were available for hepatotoxicity and nephrotoxicity in the Hazard Evaluation Support System Integrated Platform in Japan. The areas under the renal or hepatic concentration-time curves estimated using PBPK modeling were inversely associated with these lowest-observed-effect levels. Using PBPK forward dosimetry could provide the plasma/tissue concentrations of drugs and chemicals after oral dosing, thereby facilitating estimates of nephrotoxic or hepatotoxic potential as a part of the risk assessment.
    MeSH term(s) Administration, Oral ; Animals ; Computer Simulation ; Kidney/metabolism ; Liver/metabolism ; Models, Biological ; Pharmaceutical Preparations/blood ; Pharmaceutical Preparations/metabolism ; Pharmacokinetics ; Rats
    Chemical Substances Pharmaceutical Preparations
    Language English
    Publishing date 2020-06-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 639353-6
    ISSN 1520-5010 ; 0893-228X
    ISSN (online) 1520-5010
    ISSN 0893-228X
    DOI 10.1021/acs.chemrestox.0c00009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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