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  1. AU="J. Lohse"
  2. AU="Lopez-Plaza, Ileana"
  3. AU="Degorce, Sebastien L"
  4. AU="Wodchis, Walter"
  5. AU="Charles DeCarli"
  6. AU=Glied Sherry
  7. AU="Kesarwani, Manoj"
  8. AU="Cubas-Atienza, Ana I"
  9. AU="Thickens, Anna S"
  10. AU="Anderson, Emilie"
  11. AU=Starace Fabrizio
  12. AU="Ochin, Evelina"
  13. AU="Gijzen, Linda"
  14. AU="van Groeningen, Kees Jan"
  15. AU="Pakserian, Diana"
  16. AU="Joly-Chevrier, Maxine"
  17. AU="Genovesi, Piero"
  18. AU="Ersöz, Gülden"
  19. AU="Jeffrey J Babon"
  20. AU="Rehana Masood"
  21. AU="Sandra Heskamp"
  22. AU="Omid Sadeghi"
  23. AU="Antaya, Richard"
  24. AU="Papadopoulos, G"
  25. AU="Boughen, Santiago"
  26. AU="Brink, P. L."

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  1. Artikel ; Online: Cross-platform classification of level and deformed sea ice considering per-class incident angle dependency of backscatter intensity

    W. Guo / P. Itkin / J. Lohse / M. Johansson / A. P. Doulgeris

    The Cryosphere, Vol 16, Pp 237-

    2022  Band 257

    Abstract: Wide-swath C-band synthetic aperture radar (SAR) has been used for sea ice classification and estimates of sea ice drift and deformation since it first became widely available in the 1990s. Here, we examine the potential to distinguish surface features ... ...

    Abstract Wide-swath C-band synthetic aperture radar (SAR) has been used for sea ice classification and estimates of sea ice drift and deformation since it first became widely available in the 1990s. Here, we examine the potential to distinguish surface features created by sea ice deformation using ice type classification of SAR data. Also, we investigate the cross-platform transferability between training sets derived from Sentinel-1 Extra Wide (S1 EW) and RADARSAT-2 (RS2) ScanSAR Wide A (SCWA) and fine quad-polarimetric (FQ) data, as the same radiometrically calibrated backscatter coefficients are expected from the two C-band sensors. We use a novel sea ice classification method developed based on Arctic-wide S1 EW training, which considers per-ice-type incident angle (IA) dependency of backscatter intensity. This study focuses on the region near Fram Strait north of Svalbard to utilize expert knowledge of ice conditions during the Norwegian young sea ICE (N-ICE2015) expedition. Manually drawn polygons of different ice types for S1 EW, RS2 SCWA and RS2 FQ data are used to retrain the classifier. Different training sets yield similar classification results and IA slopes, with the exception of leads with calm open water, nilas or newly formed ice (the “leads” class). This is caused by different noise floor configurations of S1 and RS2 data, which interact differently with leads, necessitating dataset-specific retraining for this class. SAR scenes are then classified based on the classifier retrained for each dataset, with the classification scheme altered to separate level from deformed ice to enable direct comparison with independently derived sea ice deformation maps. The comparisons show that the classification of C-band SAR can be used to distinguish areas of ice divergence occupied by leads, young ice and level first-year ice (LFYI). However, it has limited capacity in delineating areas of ice deformation due to ambiguities between ice types with higher backscatter intensities. This study provides reference to future ...
    Schlagwörter Environmental sciences ; GE1-350 ; Geology ; QE1-996.5
    Thema/Rubrik (Code) 290
    Sprache Englisch
    Erscheinungsdatum 2022-01-01T00:00:00Z
    Verlag Copernicus Publications
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope

    Marc Bathe-Peters / Philipp Gmach / Paolo Annibale / Martin J. Lohse

    Data in Brief, Vol 29, Iss , Pp - (2020)

    2020  

    Abstract: We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup ... ...

    Abstract We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range covering at least three orders of magnitude, from 100 μm2/s down to 0.1 μm2/s. We share representative data sets covering (i) the diffusion of a dye molecule, observed in media of different viscosities and (ii) the diffusion of a prototypical membrane receptor.The data can be valuable for researchers interested in the rapid diffusion properties of nuclear, cytosolic or membrane bound proteins fused to fluorescent tags. Keywords: Confocal microscopy, Fluorescence correlation spectroscopy, Membrane receptor, Diffusion
    Schlagwörter Computer applications to medicine. Medical informatics ; R858-859.7 ; Science (General) ; Q1-390
    Sprache Englisch
    Erscheinungsdatum 2020-04-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: SARS-CoV2 wild type and mutant specific humoral and T cell immunity is superior after vaccination than after natural infection

    Jennifer R. Richardson / Ralph Götz / Vanessa Mayr / Martin J. Lohse / Hans-Peter Holthoff / Martin Ungerer

    PLoS ONE, Vol 17, Iss

    2022  Band 4

    Abstract: Objective We investigated blood samples from fully SARS-CoV2-vaccinated subjects and from previously positive tested patients up to one year after infection with SARS-CoV2, and compared short- and long-term T cell and antibody responses, with a special ... ...

    Abstract Objective We investigated blood samples from fully SARS-CoV2-vaccinated subjects and from previously positive tested patients up to one year after infection with SARS-CoV2, and compared short- and long-term T cell and antibody responses, with a special focus on the recently emerged delta variant (B.1.617.2). Methods and results In 23 vaccinated subjects, we documented high anti-SARS-CoV2 spike protein receptor binding domain (RBD) antibody titers. Average virus neutralization by antibodies, assessed as inhibition of ACE2 binding to RBD, was 2.2-fold reduced for delta mutant vs. wild type (wt) RBD. The mean specific antibody titers were lower one year after natural infection than after vaccination; ACE2 binding to delta mutant vs. wt RBD was 1.65-fold reduced. In an additional group, omicron RBD binding was reduced compared to delta. Specific CD4+ T cell responses were measured after stimulation with peptides pools from wt, alpha, beta, gamma, or delta variant SARS-CoV2 spike proteins by flow cytometric intracellular cytokine staining. There was no significant difference in cytokine production of IFN-γ, TNF-α, or IL-2 between vaccinated subjects. T cell responses to wt or mutant SARS-CoV2 spike were significantly weaker after natural occurring infections compared to those in vaccinated individuals. Conclusion Antibody neutralisation of the delta mutant was reduced compared to wt, as assessed in a novel inhibition assay with a finger prick blood drop. Strong CD4 T cell responses were present against wt and mutant SARS-CoV2 variants, including the delta (B.1.617.2) strain, in fully vaccinated individuals, whereas they were partly weaker 1 year after natural infection. Hence, immune responses after vaccination are stronger compared to those after naturally occurring infection, pointing out the need of the vaccine to overcome the pandemic.
    Schlagwörter Medicine ; R ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2022-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: SARS-CoV2 wild type and mutant specific humoral and T cell immunity is superior after vaccination than after natural infection.

    Jennifer R Richardson / Ralph Götz / Vanessa Mayr / Martin J Lohse / Hans-Peter Holthoff / Martin Ungerer

    PLoS ONE, Vol 17, Iss 4, p e

    2022  Band 0266701

    Abstract: Objective We investigated blood samples from fully SARS-CoV2-vaccinated subjects and from previously positive tested patients up to one year after infection with SARS-CoV2, and compared short- and long-term T cell and antibody responses, with a special ... ...

    Abstract Objective We investigated blood samples from fully SARS-CoV2-vaccinated subjects and from previously positive tested patients up to one year after infection with SARS-CoV2, and compared short- and long-term T cell and antibody responses, with a special focus on the recently emerged delta variant (B.1.617.2). Methods and results In 23 vaccinated subjects, we documented high anti-SARS-CoV2 spike protein receptor binding domain (RBD) antibody titers. Average virus neutralization by antibodies, assessed as inhibition of ACE2 binding to RBD, was 2.2-fold reduced for delta mutant vs. wild type (wt) RBD. The mean specific antibody titers were lower one year after natural infection than after vaccination; ACE2 binding to delta mutant vs. wt RBD was 1.65-fold reduced. In an additional group, omicron RBD binding was reduced compared to delta. Specific CD4+ T cell responses were measured after stimulation with peptides pools from wt, alpha, beta, gamma, or delta variant SARS-CoV2 spike proteins by flow cytometric intracellular cytokine staining. There was no significant difference in cytokine production of IFN-γ, TNF-α, or IL-2 between vaccinated subjects. T cell responses to wt or mutant SARS-CoV2 spike were significantly weaker after natural occurring infections compared to those in vaccinated individuals. Conclusion Antibody neutralisation of the delta mutant was reduced compared to wt, as assessed in a novel inhibition assay with a finger prick blood drop. Strong CD4 T cell responses were present against wt and mutant SARS-CoV2 variants, including the delta (B.1.617.2) strain, in fully vaccinated individuals, whereas they were partly weaker 1 year after natural infection. Hence, immune responses after vaccination are stronger compared to those after naturally occurring infection, pointing out the need of the vaccine to overcome the pandemic.
    Schlagwörter Medicine ; R ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2022-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Publisher Correction

    Amod Godbole / Sandra Lyga / Martin J. Lohse / Davide Calebiro

    Nature Communications, Vol 9, Iss 1, Pp 1-

    Internalized TSH receptors en route to the TGN induce local Gs-protein signaling and gene transcription

    2018  Band 1

    Abstract: The original version of this Article contained errors in the three equations reported in the Methods section entitled ‘Statistics’, as described in the accompanying Publisher Correction. These errors have been corrected in both the PDF and HTML versions ... ...

    Abstract The original version of this Article contained errors in the three equations reported in the Methods section entitled ‘Statistics’, as described in the accompanying Publisher Correction. These errors have been corrected in both the PDF and HTML versions of the article.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-12-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: Publisher Correction

    Amod Godbole / Sandra Lyga / Martin J. Lohse / Davide Calebiro

    Nature Communications, Vol 9, Iss 1, Pp 1-

    Internalized TSH receptors en route to the TGN induce local Gs-protein signaling and gene transcription

    2018  Band 1

    Abstract: The original version of this Article contained errors in the three equations reported in the Methods section entitled ‘Statistics’, as described in the accompanying Publisher Correction. These errors have been corrected in both the PDF and HTML versions ... ...

    Abstract The original version of this Article contained errors in the three equations reported in the Methods section entitled ‘Statistics’, as described in the accompanying Publisher Correction. These errors have been corrected in both the PDF and HTML versions of the article.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-12-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: Internalized TSH receptors en route to the TGN induce local Gs-protein signaling and gene transcription

    Amod Godbole / Sandra Lyga / Martin J. Lohse / Davide Calebiro

    Nature Communications, Vol 8, Iss 1, Pp 1-

    2017  Band 15

    Abstract: Recent investigations suggest that G-protein-coupled receptors (GPCRs) can signal during intracellular trafficking. Here the authors use fluorescence microscopy approaches to directly visualize and investigate functional consequences of GPCR-mediated ... ...

    Abstract Recent investigations suggest that G-protein-coupled receptors (GPCRs) can signal during intracellular trafficking. Here the authors use fluorescence microscopy approaches to directly visualize and investigate functional consequences of GPCR-mediated signaling at the Golgi/trans-Golgi network.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2017-09-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Internalized TSH receptors en route to the TGN induce local Gs-protein signaling and gene transcription

    Amod Godbole / Sandra Lyga / Martin J. Lohse / Davide Calebiro

    Nature Communications, Vol 8, Iss 1, Pp 1-

    2017  Band 15

    Abstract: Recent investigations suggest that G-protein-coupled receptors (GPCRs) can signal during intracellular trafficking. Here the authors use fluorescence microscopy approaches to directly visualize and investigate functional consequences of GPCR-mediated ... ...

    Abstract Recent investigations suggest that G-protein-coupled receptors (GPCRs) can signal during intracellular trafficking. Here the authors use fluorescence microscopy approaches to directly visualize and investigate functional consequences of GPCR-mediated signaling at the Golgi/trans-Golgi network.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2017-09-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  9. Artikel ; Online: Unraveling the hidden temporal range of fast β2-adrenergic receptor mobility by time-resolved fluorescence

    Ashwin Balakrishnan / Katherina Hemmen / Susobhan Choudhury / Jan-Hagen Krohn / Kerstin Jansen / Mike Friedrich / Gerti Beliu / Markus Sauer / Martin J. Lohse / Katrin G. Heinze

    Communications Biology, Vol 5, Iss 1, Pp 1-

    2022  Band 13

    Abstract: The mobility of the β2-adrenergic receptor, from the nanosecond to the second range, is revealed by combining several fluorescent spectroscopy techniques. These data also show a previously hidden mobility of this GPCR. ...

    Abstract The mobility of the β2-adrenergic receptor, from the nanosecond to the second range, is revealed by combining several fluorescent spectroscopy techniques. These data also show a previously hidden mobility of this GPCR.
    Schlagwörter Biology (General) ; QH301-705.5
    Sprache Englisch
    Erscheinungsdatum 2022-02-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  10. Artikel ; Online: Pharmacological Characterization of the Stick Insect Carausius morosus Allatostatin‑C Receptor with Its Endogenous Agonist

    Ali Işbilir / Burcin Duan Sahbaz / Gunes Tuncgenc / Moritz Bünemann / Martin J. Lohse / Necla Birgül-Iyison

    ACS Omega, Vol 5, Iss 50, Pp 32183-

    2020  Band 32194

    Schlagwörter Chemistry ; QD1-999
    Sprache Englisch
    Erscheinungsdatum 2020-12-01T00:00:00Z
    Verlag American Chemical Society
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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