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  1. Article ; Online: Corrigendum to ‘Simpler and faster Covid-19 testing

    Nuttada Panpradist / Qin Wang / Parker S. Ruth / Jack H. Kotnik / Amy K. Oreskovic / Abraham Miller / Samuel W.A. Stewart / Justin Vrana / Peter D. Han / Ingrid A. Beck / Lea M. Starita / Lisa M. Frenkel / Barry R. Lutz

    EBioMedicine, Vol 66, Iss , Pp 103296- (2021)

    Strategies to streamline SARS-CoV-2 molecular assays’

    2021  

    Keywords Medicine ; R ; Medicine (General) ; R5-920
    Language English
    Publishing date 2021-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Simpler and faster Covid-19 testing

    Nuttada Panpradist / Qin Wang / Parker S. Ruth / Jack H. Kotnik / Amy K. Oreskovic / Abraham Miller / Samuel W.A. Stewart / Justin Vrana / Peter D. Han / Ingrid A. Beck / Lea M. Starita / Lisa M. Frenkel / Barry R. Lutz

    EBioMedicine, Vol 64, Iss , Pp 103236- (2021)

    Strategies to streamline SARS-CoV-2 molecular assays

    2021  

    Abstract: Background: Detection of SARS-CoV-2 infections is important for treatment, isolation of infected and exposed individuals, and contact tracing. RT-qPCR is the “gold-standard” method to sensitively detect SARS-CoV-2 RNA, but most laboratory-developed RT- ... ...

    Abstract Background: Detection of SARS-CoV-2 infections is important for treatment, isolation of infected and exposed individuals, and contact tracing. RT-qPCR is the “gold-standard” method to sensitively detect SARS-CoV-2 RNA, but most laboratory-developed RT-qPCR assays involve complex steps. Here, we aimed to simplify RT-qPCR assays by streamlining reaction setup, eliminating RNA extraction, and proposing reduced-cost detection workflows that avoid the need for expensive qPCR instruments. Method: A low-cost RT-PCR based “kit” was developed for faster turnaround than the CDC developed protocol. We demonstrated three detection workflows: two that can be deployed in laboratories conducting assays of variable complexity, and one that could be simple enough for point-of-care. Analytical sensitivity was assessed using SARS-CoV-2 RNA spiked in simulated nasal matrix. Clinical performance was evaluated using contrived human nasal matrix (n = 41) and clinical nasal specimens collected from individuals with respiratory symptoms (n = 110). Finding: The analytical sensitivity of the lyophilised RT-PCR was 10 copies/reaction using purified SARS-CoV-2 RNA, and 20 copies/reaction when using direct lysate in simulated nasal matrix. Evaluation of assay performance on contrived human matrix showed 96.7–100% specificity and 100% sensitivity at ≥20 RNA copies. A head-to-head comparison with the standard CDC protocol on clinical specimens showed 83.8–94.6% sensitivity and 96.8–100% specificity. We found 3.6% indeterminate samples (undetected human control), lower than 8.1% with the standard protocol. Interpretation: This preliminary work should support laboratories or commercial entities to develop and expand access to Covid-19 testing. Software guidance development for this assay is ongoing to enable implementation in other settings. Fund: USA NIH R01AI140845 and Seattle Children's Research Institute
    Keywords Low-cost Covid-19 testing ; Fast Covid-19 testing ; Point-of-care ; Medicine ; R ; Medicine (General) ; R5-920
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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