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  1. Article ; Online: Identification, isolation and analysis of human gut-associated lymphoid tissues.

    Jørgensen, Peter B / Fenton, Thomas M / Mörbe, Urs M / Riis, Lene B / Jakobsen, Henrik L / Nielsen, Ole H / Agace, William W

    Nature protocols

    2021  Volume 16, Issue 4, Page(s) 2051–2067

    Abstract: Gut-associated lymphoid tissues (GALTs) comprise key intestinal immune inductive sites, including the Peyer's patches of the small intestine and different types of isolated lymphoid follicle (ILF) found along the length of the gut. Our understanding of ... ...

    Abstract Gut-associated lymphoid tissues (GALTs) comprise key intestinal immune inductive sites, including the Peyer's patches of the small intestine and different types of isolated lymphoid follicle (ILF) found along the length of the gut. Our understanding of human GALT is limited due to a lack of protocols for their isolation. Here we describe a technique that, uniquely among intestinal cell isolation protocols, allows identification and isolation of all human GALT, as well as GALT-free intestinal lamina propria (LP). The technique involves the mechanical separation of intestinal mucosa from the submucosa, allowing the identification and isolation of submucosal ILF (SM-ILF), LP-embedded mucosal ILF (M-ILF) and LP free of contaminating lymphoid tissue. Individual SM-ILF, M-ILF and Peyer's patch follicles can be subsequently digested for downstream cellular and molecular characterization. The technique, which takes 4-10 h, will be useful for researchers interested in intestinal immune development and function in health and disease.
    MeSH term(s) Cell Count ; Cell Survival ; Colon/physiology ; Crohn Disease/pathology ; Gastrointestinal Tract/physiology ; Humans ; Immunity, Innate ; Intestinal Mucosa/cytology ; Leukocyte Common Antigens/metabolism ; Lymphoid Tissue/physiology ; Tissue Culture Techniques/methods
    Chemical Substances Leukocyte Common Antigens (EC 3.1.3.48)
    Language English
    Publishing date 2021-02-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2244966-8
    ISSN 1750-2799 ; 1754-2189
    ISSN (online) 1750-2799
    ISSN 1754-2189
    DOI 10.1038/s41596-020-00482-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Combined endoscopic and laparoscopic surgery (CELS) for early colon cancer in high-risk patients.

    Hartwig, Morten F S / Bulut, Mustafa / Ravn-Eriksen, Jens / Hansen, Lasse B / Bojesen, Rasmus D / Klein, Mads Falk / Jakobsen, Henrik L / Rasmussen, Morten / Rud, Bo / Eriksen, Jens-Ole / Eiholm, Susanne / Fiehn, Anne-Marie K / Quirke, Phil / Gögenur, Ismail

    Surgical endoscopy

    2023  Volume 37, Issue 11, Page(s) 8511–8521

    Abstract: Background: Local excision of early colon cancers could be an option in selected patients with high risk of complications and no sign of lymph node metastasis (LNM). The primary aim was to assess feasibility in high-risk patients with early colon cancer ...

    Abstract Background: Local excision of early colon cancers could be an option in selected patients with high risk of complications and no sign of lymph node metastasis (LNM). The primary aim was to assess feasibility in high-risk patients with early colon cancer treated with Combined Endoscopic and Laparoscopic Surgery (CELS).
    Methods: A non-randomized prospective feasibility study including 25 patients with Performance Status score ≥ 1 and/or American Society of Anesthesiologists score ≥ 3, and clinical Union of International Cancer Control stage-1 colon cancer suitable for CELS resection. The primary outcome was failure of CELS resection, defined as either: Incomplete resection (R1/R2), local recurrence within 3 months, complication related to CELS within 30 days (Clavien-Dindo grade ≥ 3), death within 30 days or death within 90 days due to complications to surgery.
    Results: Fifteen patients with clinical T1 (cT1) and ten with clinical T2 (cT2) colon cancer and without suspicion of metastases were included. Failure occurred in two patients due to incomplete resections. Histopathological examination classified seven patients as having pT1, nine as pT2, six as pT3 adenocarcinomas, and three as non-invasive tumors. In three patients, the surgical strategy was changed intraoperatively to conventional colectomy due to tumor location or size. Median length of stay was 1 day. Seven patients had completion colectomy performed due to histological high-risk factors. None had LNM.
    Conclusions: In selected patients, CELS resection was feasible, and could spare some patients large bowel resection.
    MeSH term(s) Humans ; Abdomen/surgery ; Colectomy ; Colonic Neoplasms/surgery ; Laparoscopy ; Prospective Studies ; Retrospective Studies ; Treatment Outcome ; Feasibility Studies
    Language English
    Publishing date 2023-09-28
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 639039-0
    ISSN 1432-2218 ; 0930-2794
    ISSN (online) 1432-2218
    ISSN 0930-2794
    DOI 10.1007/s00464-023-10385-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2214: Show issues Location:
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    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Geriatric assessment and intervention in older vulnerable patients undergoing surgery for colorectal cancer: a protocol for a randomised controlled trial (GEPOC trial).

    Dolin, Troels G / Mikkelsen, Marta / Jakobsen, Henrik L / Nordentoft, Tyge / Pedersen, Trine S / Vinther, Anders / Zerahn, Bo / Vistisen, Kirsten K / Suetta, Charlotte / Nielsen, Dorte / Johansen, Julia S / Lund, Cecilia M

    BMC geriatrics

    2021  Volume 21, Issue 1, Page(s) 88

    Abstract: Background: The incidence of colorectal cancer (CRC) increases with age. Older patients are a heterogeneous group ranging from fit to frail with various comorbidities. Frail older patients with CRC are at increased risk of negative outcomes and ... ...

    Abstract Background: The incidence of colorectal cancer (CRC) increases with age. Older patients are a heterogeneous group ranging from fit to frail with various comorbidities. Frail older patients with CRC are at increased risk of negative outcomes and functional decline after cancer surgery compared to younger and fit older patients. Maintenance of independence after treatment is rarely investigated in clinical trials despite older patients value it as high as survival. Comprehensive geriatric assessment (CGA) is an evaluation of an older persons' medical, psychosocial, and functional capabilities to develop an overall plan for treatment and follow-up. The beneficial effect of CGA is well documented in the fields of medicine and orthopaedic surgery, but evidence is lacking in cancer surgery. We aim to investigate the effect of CGA on physical performance in older frail patients undergoing surgery for CRC.
    Methods: GEPOC is a single centre randomised controlled trial including older patients (≥65 years) undergoing surgical resection for primary CRC. Frail patients (≤14/17 points using the G8 screening tool) will be randomised 1:1 to geriatric intervention and exercise (n = 50) or standard of care along (n = 50) with their standard surgical procedure. Intervention includes preoperative CGA, perioperative geriatric in-ward review and postoperative follow-up. All patients in the intervention group will participate in a pre- and postoperative resistance exercise programme (twice/week, 2 + 12 weeks). Primary endpoint is change in 30-s chair stand test. Assessment of primary endpoint will be performed by physiotherapists blinded to patient allocation. Secondary endpoints: changes in health related quality of life, physical strength and capacity (handgrip strength, gait speed and 6 min walking test), patient perceived quality of recovery, complications to surgery, body composition (Dual-energy X-ray absorptiometry and bioelectric impedance), serum biomarkers, readmission, length of stay and survival.
    Discussion: This ongoing trial will provide valuable knowledge on whether preoperative CGA and postoperative geriatric follow-up and intervention including an exercise program can counteract physical decline and improve quality of life in frail CRC patients undergoing surgery.
    Trial registration: Prospectively registered at Clinicaltrials.gov NCT03719573 (October 2018).
    MeSH term(s) Aged ; Aged, 80 and over ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/surgery ; Exercise ; Geriatric Assessment ; Hand Strength ; Humans ; Quality of Life ; Randomized Controlled Trials as Topic
    Language English
    Publishing date 2021-01-30
    Publishing country England
    Document type Clinical Trial Protocol ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2059865-8
    ISSN 1471-2318 ; 1471-2318
    ISSN (online) 1471-2318
    ISSN 1471-2318
    DOI 10.1186/s12877-021-02045-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity.

    Fenton, Thomas M / Jørgensen, Peter B / Niss, Kristoffer / Rubin, Samuel J S / Mörbe, Urs M / Riis, Lene B / Da Silva, Clément / Plumb, Adam / Vandamme, Julien / Jakobsen, Henrik L / Brunak, Søren / Habtezion, Aida / Nielsen, Ole H / Johansson-Lindbom, Bengt / Agace, William W

    Immunity

    2020  Volume 52, Issue 3, Page(s) 557–570.e6

    Abstract: The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in ... ...

    Abstract The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1
    MeSH term(s) Adaptive Immunity/genetics ; Adaptive Immunity/immunology ; Animals ; Flow Cytometry ; Gastric Mucosa/immunology ; Gastric Mucosa/metabolism ; Gastric Mucosa/ultrastructure ; Humans ; Immunity, Mucosal/genetics ; Immunity, Mucosal/immunology ; Immunoglobulin A/genetics ; Immunoglobulin A/immunology ; Immunoglobulin M/genetics ; Immunoglobulin M/immunology ; Intestinal Mucosa/immunology ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/ultrastructure ; Intestines/immunology ; Intestines/ultrastructure ; Lymphocytes/immunology ; Lymphocytes/metabolism ; Lymphoid Tissue/immunology ; Lymphoid Tissue/metabolism ; Lymphoid Tissue/ultrastructure ; Microscopy, Confocal ; Microscopy, Electron, Scanning ; Peyer's Patches/immunology ; Peyer's Patches/metabolism ; Peyer's Patches/ultrastructure ; Sequence Analysis, DNA
    Chemical Substances Immunoglobulin A ; Immunoglobulin M
    Language English
    Publishing date 2020-03-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1217235-2
    ISSN 1097-4180 ; 1074-7613
    ISSN (online) 1097-4180
    ISSN 1074-7613
    DOI 10.1016/j.immuni.2020.02.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4227: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 69: Show issues

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