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Article ; Online: Phylogeographic characterization of Burkholderia pseudomallei isolated from Bangladesh.

Md Shariful Alam Jilani / Saika Farook / Arittra Bhattacharjee / Lovely Barai / Chowdhury Rafiqul Ahsan / Jalaluddin Ashraful Haq / Apichai Tuanyok

PLoS Neglected Tropical Diseases, Vol 17, Iss 12, p e

2023 Volume 0011823

Abstract: Background Burkholderia pseudomallei possesses a diverse set of genes which encode a vast array of biological functions reflecting its clinical, ecological and phenotypic diversity. Strain variation is linked to geographic location as well as pattern of ... ...

Abstract	Background Burkholderia pseudomallei possesses a diverse set of genes which encode a vast array of biological functions reflecting its clinical, ecological and phenotypic diversity. Strain variation is linked to geographic location as well as pattern of land uses. This soil-dwelling Gram-negative pathogen causes melioidosis, a tropical disease endemic in northern Australia and Southeast Asian regions including Bangladesh. Phylogeographic analyses of B. pseudomallei isolates by molecular typing techniques could be used to examine the diversity of this organism as well as to track melioidosis epidemics. Methods In this study, 22 B. pseudomallei isolates, of which 20 clinical and two soil isolates were analyzed, utilizing Real-time PCR assay and multilocus sequence typing (MLST). The sequences were then submitted to PubMLST database for analysis and construction of phylogenetic tree. Findings A total of 12 different sequence types (STs) that includes four novel STs were identified for the first time. Strains having STs 1005, 1007 and 56 were the most widespread STs frequently isolated in Bangladesh. ST 1005, ST 56, ST 1007 and ST 211 have been detected not only in Bangladesh but are also present in many Southeast Asian countries. Significance ST 1005 was detected in both soil and clinical samples of Gazipur. Most prevalent, ST 56 has been previously reported from Myanmar, Thailand, Cambodia and Vietnam, confirming the persistence of the genotype over the entire continent. Further large-scale study is necessary to find out the magnitude of the infection and its different reservoirs in the environment along with phylogeographic association.
Keywords	Arctic medicine. Tropical medicine ; RC955-962 ; Public aspects of medicine ; RA1-1270
Subject code	630
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Helicobacter pylori infection in asymptomatic rural Bangladeshi population

Mir Masudur Rhaman / Fahmida Rahman / Sraboni Mazumder / M. Abu Sayeed / Jalaluddin Ashraful Haq

IMC Journal of Medical Science, Vol 15, Iss 1, Pp 1-

2021 Volume 6

Abstract: ... diabetes. IMC J Med Sci 2021; 15(1): 007. OPEN ACCESS *Correspondence: Jalaluddin Ashraful Haq, Department ...

Abstract	Background and objectives: The prevalence of Helicobacter pylori infection differs in urban and rural population. In our country, no previous study investigated the H. pylori infection in rural population. The aim of the present study was to find out the status of H. pylori infection among the Bangladeshi asymptomatic rural adult population. Material and Methods: This cross-sectional study was carried out in a rural area located about 40 km north-east of capital Dhaka. Apparently healthy non-diabetic, pre-diabetic and diabetic adults (18 years and above) were enrolled in this study. A structured questionnaire was developed to record the socio-demographic and clinical information. H. pylori infection status was determined by the presence of anti- H. pylori IgG antibody in blood. Serum anti-H.pylori IgG antibodies were determined by immunochromatographic test (ICT) method. Results: A total number of 180 apparently healthy adult individuals were enrolled of which 112, 40 and 28 were non-diabetic, pre-diabetic and diabetic respectively. Out of 180 individuals, anti- H. pylori IgG was present in 70 (38.9%, CI: 32.1, 46.2) cases. Infection rate was 50%, 27.5% and 43.5% in 19-30, 31-50 and >50 years age group respectively. Infection rate was significantly (p< 0.05) low in 31-50 years age group compared to 19-30 and > 50 years age groups. H. pylori infection rates in male and female were 42.6% (CI: 29.2, 56.8) and 37.3% (CI: 28.9, 46.4) respectively (p=0.50). There was no significant (p>0.05) association of H. pylori infection with economic status, education level, occupation and tobacco consumption of the study population. The rate of H. pylori infection in non-diabetic, pre-diabetic and diabetic individuals were not significantly different from each other. Conclusion: The study revealed a low prevalence of H. pylori infection in rural population of Bangladesh. There was no significant association of H. pylori infection with several sociodemographic status and diabetes. IMC J Med Sci 2021; 15(1): 007. OPEN ACCESS *Correspondence: Jalaluddin Ashraful Haq, Department of Microbiology, Ibrahim Medical College, 1/A Ibrahim Sarani, Segunbagicha, Dhaka 1000, Bangladesh. Email: jahaq54@yahoo.com
Keywords	Medicine ; R
Subject code	360
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	Ibrahim Medical College
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Antibody response to receptor-binding domain of SARS-CoV-2 spike protein following vaccination and natural infection with SARS-CoV-2

Fahmida Rahman / Sraboni Mazumder / Saika Farook / Paroma Deb / Supti Prava Saha / Farjana Akter / Md Shariful Alam Jilani / Jalaluddin Ashraful Haq

IMC Journal of Medical Science, Vol 17, Iss 1, Pp 1-

2022 Volume 7

Abstract: Background and objectives: Antibody to SARS-CoV-2 develops both after natural infection with SARS-CoV-2 and vaccination. This study was undertaken to determine the antibody response to SARS-CoV-2 among population after natural SARS-CoV-2 infection and ... ...

Abstract	Background and objectives: Antibody to SARS-CoV-2 develops both after natural infection with SARS-CoV-2 and vaccination. This study was undertaken to determine the antibody response to SARS-CoV-2 among population after natural SARS-CoV-2 infection and vaccination. Material and methods: The study was carried out on adults aged 18 years and above. Study population consisted of four groups. Group-1 (control): healthy and history of no prior SARS-CoV-2 infection and vaccination, Group-2: had past SARS-CoV-2 infection and no vaccination, Group-3: received two doses of recombinant adenoviral vector vaccine ChAdOx1 (Oxford–AstraZeneca) without past SARS-CoV-2 infection, and Group-4: had past SARS-CoV-2 infection and received 2 doses of ChAdOx1 vaccination. Blood was collected 1 and 7 months after the second dose of vaccination from Group-3 and 4 individuals. Single blood sample was collected from participants of Gr-1 and 2 at the time of enrolment. Immunoglobulin G (IgG) antibodies to receptor-binding domain (RBD) of SARS-CoV-2 spike protein S1 (anti-RBDS1 IgG) was determined in serum by ELISA method. Results: Total 176 participants aged 18 years and above were enrolled. Anti-RBDS1 IgG positivity rates were 51.9%, 66.7%, 96.8% and 100% in individuals of Group-1, 2, 3 and 4 respectively. Gr-4 had significantly (p < 0.05) mean higher anti-RBDS1 IgG antibody level (120.8 ± 31.9 DU/ml) compared to other groups 1 month after 2nd dose of vaccination. No significant differences in antibody response were found among the individuals of four groups across gender and comorbidities. Seven months after the 2nd dose of vaccines, the antibody concentration declined in 85.3% (112.1 ± 30.4 DU/ml to 75.9 ± 48.7 DU/ml) and 81.5% (127.3 ± 20.4 DU/ml to 92.5 ± 43.6 DU/ml) individuals of Group-3 and Group-4 respectively. Decline of antibody was 40.6% and 34.7% in 7 months, but all remained positive except 1 in Group-3. Fever (34.4%) and headache (24.8%) were the most common adverse effects noted after vaccination. Conclusion: The study ...
Keywords	Medicine ; R
Subject code	630
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	Ibrahim Medical College
Document type	Article ; Online
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Article ; Online: Helicobacter pylori CagA seropositivity in adult Bangladeshi patients with peptic ulcer and erosion

Fahmida Rahman / Khandaker Shadia / Salma Khatun / Mafruha Mahmud / Indrajit Kumar Dutta / Jalaluddin Ashraful Haq

IMC Journal of Medical Science, Vol 14, Iss 1, Pp 1-

2020 Volume 5

Abstract: Background: CagA IgG antibody in sera might indicate presence of virulent Helicobacter pylori in patients with peptic ulcer disease. Present study was performed to find out the prevalence of CagA IgG antibody in patients with peptic ulcer/erosion. ... ...

Abstract	Background: CagA IgG antibody in sera might indicate presence of virulent Helicobacter pylori in patients with peptic ulcer disease. Present study was performed to find out the prevalence of CagA IgG antibody in patients with peptic ulcer/erosion. Methods: Any case that had peptic ulcer/erosion, plus positive for rapid urease test (RUT) or H. pylori stool antigen (HpSAg) or serum anti-H. pylori IgG/IgA were included in the study and named as H. pylori positive case. H. pylori positive cases were tested for CagA IgG antibody. Anti-H. pylori IgG, IgA and CagA IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA) and stool antigen by rapid immunochromatographic test (ICT). Urease production in biopsy sample was detected by RUT. Results: Total 86 H. pylori positive patients were included in the study. Out of 86 patients, CagA IgG was positive in 34 (39.5%; 95% CI: 0.30,0.50) cases. CagA seropositivity rate in ulcer and erosion cases were 58.8% (95% CI: 0.36,0.78) and 34.8% (95% CI: 0.25,0.47) respectively. H. pylori stool antigen and IgA antibodies were positive in all (100%) CagA antibody positive ulcer cases while the rates were significantly less among the CagA antibody negative cases (42.8% and 28.6%; p<0.05). However, in CagA antibody positive erosion cases, the rates were not significantly different from CagA antibody negative cases. Conclusion: The study has demonstrated that the CagA positive strain is less prevalent in erosion than ulcer cases. IMC J Med Sci 2020; 14(1): 006. EPub date: 05 April 2020 *Correspondence: J. Ashraful Haq, Department of Microbiology, Ibrahim Medical College, 1/A Ibrahim Sarani, Segunbagicha, Dhaka 1000, Bangladesh. Email: jahaq54@yahoo.com 2a: present address
Keywords	Medicine ; R
Subject code	630
Language	English
Publishing date	2020-06-01T00:00:00Z
Publisher	Ibrahim Medical College
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Helicobacter pylori infection in diabetes mellitus patients with peptic ulcer disease

Salma Khatun / Khandaker Shadia / Mafruha Mahmud / Sraboni Mazumder / Indrajit Kumar Dutta / Fahmida Rahman / Md. Shariful Alam Jilani / Jalaluddin Ashraful Haq

IMC Journal of Medical Science, Vol 14, Iss 2, Pp 1-

2021 Volume 6

Abstract: Background and objectives: Helicobacter pylori infection is suspected to be associated with extra-gastrointestinal disorders such as diabetes mellitus (DM). It is still a subject of investigation whether H. pylori has a pathogenic role on DM or diabetic ... ...

Abstract	Background and objectives: Helicobacter pylori infection is suspected to be associated with extra-gastrointestinal disorders such as diabetes mellitus (DM). It is still a subject of investigation whether H. pylori has a pathogenic role on DM or diabetic patients have an increased susceptibility to H. pylori infection. The aim of the present study was to find out the rate of H. pylori infection in individuals with and without DM. Materials and methods: The study was conducted on 72 diabetic and 19 non-diabetic adult individuals with dyspeptic symptoms attending the BIRDEM General Hospital for diagnostic endoscopy. All cases were tested for H. pylori stool antigen by rapid immunochromatographic test (ICT), urease production in biopsy samples by rapid urease test (RUT), and serum anti-H. pylori IgA and anti-CagA IgG antibodies by enzyme-linked immunosorbent assay (ELISA). Any case that had peptic ulcer/erosion and was positive for H. pylori stool antigen or rapid urease test (RUT) was defined as H. pylori positive case. Results: There was no significant (p=0.095) difference in H. pylori infection between diabetics and non-diabetics (68.1% vs 47.4%). Presence of ulcer and erosion were not significantly different among diabetics and non-diabetics. Anti-H. pylori IgA positivity rate in H. pylori positive diabetic and non-diabetic cases were 65.3% and 55.6% (p=0.575) respectively while anti-CagA IgG rate in those cases were 46.9% and 66.7% (p=0.276) respectively. Conclusion: The present study did not reveal any significant difference in H. pylori infection between individuals with and without DM having peptic ulcer/erosion. IMC J Med Sci 2020; 14(2): 006. EPub date: 17 January 2021. OPEN ACCESS *Correspondence: J. Ashraful Haq, Department of Microbiology, Ibrahim Medical College, 1/A Ibrahim Sarani, Segunbagicha, Dhaka 1000, Bangladesh. Email: jahaq54@yahoo.com
Keywords	Medicine ; R
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Ibrahim Medical College
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Detection of Candida auris and its antifungal susceptibility

Subarna Dutta / Md. Hasibur Rahman / Kazi Shakhawath Hossain / Jalaluddin Ashraful Haq

IMC Journal of Medical Science, Vol 13, Iss 2, Pp 1-

first report from Bangladesh

2019 Volume 5

Abstract: ... 2019; 13(2): 003. EPub date: 05 August 2019 Address for Correspondence: Jalaluddin Ashraful Haq ...

Abstract	Background and objectives: Candida auris is an emerging multidrug-resistant fungal pathogen that has been associated with nosocomial infections with a high mortality. The organism has been reported from several countries of the world except Bangladesh. The present study describes the presence of C. auris in clinical samples obtained from a large hospital of Dhaka city, Bangladesh. Materials and methods: The A total of 100 Candida species isolated from different clinical samples were purposively included in the present study. Samples were obtained from patients attending a 750 bed hospital of Dhaka city. C. auris was identified by growth characteristics, biochemical and carbohydrate assimilation test and further confirmed by polymerase chain reaction and sequencing using ITS1 and ITS2 targeting the conserved regions of 5.8S rRNA. Antifungal susceptibility of identified C. auris was performed by disk diffusion and minimum inhibitory concentration (MIC) methods. Results: Out of 100 Candida sp. tested, 21 isolates were identified as C. auris. Of the 21 C. auris, 14 (66.7%) were isolated from blood samples and the remaining 7 (33.4%) were from urine. Most of the C. auris isolated were from patients admitted in intensive care units. Out of 21 C. auris, 17 (81.0%), 7 (33.3%) and 3 (14.3%) were sensitive to amphotericin B, fluconazole and voriconazole respectively by disk diffusion method. Out of 14 fluconazole resistant isolates, 5 were susceptible dose-dependent (SS-D) by MIC method. Conclusion: The present study is the first report demonstrating the presence of C. auris in clinical samples obtained from a large hospital of Bangladesh. Majority of isolates showed resistance to fluconazole and variable susceptibility to other antifungal agents. Further study is suggested to find its true magnitude and its susceptibility pattern to a range of antifungal agents. IMC J Med Sci 2019; 13(2): 003. EPub date: 05 August 2019 Address for Correspondence: Jalaluddin Ashraful Haq, Professor of Microbiology, Ibrahim Medical College, 122 Kazi Nazrul Islam Avenue, Shahbag, Dhaka 1000, Bangladesh. Email: jahaq54@yahoo.com
Keywords	Medicine ; R
Subject code	610
Language	English
Publishing date	2019-08-01T00:00:00Z
Publisher	Ibrahim Medical College
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Asymptomatic Helicobacter pylori infection among rural children and adolescents in Bangladesh

Sraboni Mazumder / Fahmida Rahman / Farjana Akter / Rehana Khatun / Shahida Akter / Supti Prava Saha / Md. Shariful Alam Jilani / Mohammad Abu Sayeed / Jalaluddin Ashraful Haq

IMC Journal of Medical Science, Vol 16, Iss 2, Pp 1-

2022 Volume 8

Abstract: Background and objectives: The Helicobacter pylori infection rate varies according to the age, location of the residence and socioeconomic status. The aim of the present study was to investigate the status of H. pylori infection among the asymptomatic ... ...

Abstract	Background and objectives: The Helicobacter pylori infection rate varies according to the age, location of the residence and socioeconomic status. The aim of the present study was to investigate the status of H. pylori infection among the asymptomatic Bangladeshi rural children and adolescents. Material and methods: This cross-sectional study was carried out in a rural area under Pabna district about 150 km north-west of capital Dhaka. Asymptomatic and apparently healthy rural children and adolescents aged 6 to 18 years were enrolled in the study. A structured questionnaire was used to record the socio-demographic and clinical information. The rate of H. pylori infection was determined by the presence of H. pylori antigen in faeces and/or anti-H. pylori IgG and/or IgA antibodies in blood. H. pylori stool antigen was detected by lateral flow chromatographic immunoassay and serum anti-H. pylori IgG and IgA antibodies were estimated by ELISA method. Results: A total number of 185 asymptomatic and apparently healthy children and adolescents were enrolled of which 34, 131 and 20 were in 6-10, 11-15 and 16-18 years age groups respectively. The overall H. pylori infection rate was 79.5% (95% CI: 0.729, 0.85) by positive stool antigen or by the presence of serum anti-H. pylori IgG/IgA antibodies. The rate of H. pylori infection significantly (p=0.05) increased with progress of age. H. pylori infection rate was 67.6%, 80.2% and 95% in 6-10, 11-15 and 16-18 years age groups respectively. The concentration of serum anti-H. pylori IgG/IgA antibodies did not differ across the age groups. The infection rate was significantly (p<0.05) higher among the children of illiterate parents compared to the children of literate parents. Conclusion: The study demonstrated a high prevalence of H. pylori infection among children and adolescents in a rural setting. Gender and family history did not affect H. pylori prevalence but increasing age and poor educational status of parents were associated with a higher H. pylori prevalence. IMC ...
Keywords	Medicine ; R
Subject code	360
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Ibrahim Medical College
Document type	Article ; Online
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Article ; Online: Detection of antibodies to recombinant truncated flagellin and sonicated whole cell antigen of Burkholderia pseudomallei in acute melioidosis and in healthy Bangladeshi individuals

Md. Shariful Alam Jilani / Tang Thean Hock / Sraboni Mazumder / Fahmida Rahman / Md. Mohiuddin / Chowdhury Rafiqul Ahsan / Jalaluddin Ashraful Haq

IMC Journal of Medical Science, Vol 14, Iss 1, Pp 1-

2020 Volume 6

Abstract: Background and objectives: Several types of Burkholderia pseudomallei antigens have been used to determine the antibody response in acute and asymptomatic cases. In the present study, we have detected immunoglobulin G (IgG) antibody to recombinant ... ...

Abstract	Background and objectives: Several types of Burkholderia pseudomallei antigens have been used to determine the antibody response in acute and asymptomatic cases. In the present study, we have detected immunoglobulin G (IgG) antibody to recombinant truncated flagellin antigen (RTFA) of B. pseudomallei in the sera of acute melioidosis cases and healthy individuals from melioidosis endemic areas of Bangladesh by indirect enzyme-linked immunosorbent assay (ELISA). In parallel, IgG antibody to sonicated whole cell antigen (SWCA) of B. pseudomallei was determined to compare with anti-RTFA antibody. Methodology: Serum samples from culture confirmed melioidosis cases and from healthy individuals aged 21 years and above residing in melioidosis endemic rural areas were included in the study. Serum IgG antibody to RTFA and SWCA of B. pseudomallei was determined by indirect ELISA. Results: Out of 8 culture confirmed acute melioidosis cases, 7 (87.5%) and 8 (100%) were positive for anti-B. pseudomallei IgG antibodies by RTFA and SWCA methods respectively. Among 361 healthy individuals, the rate of seropositivity by RTFA-ELISA was significantly less than that of SWCA-ELISA (16.1% versus 26.8%; p = 0.001). The mean optical density (OD) of RTFA-ELISA of positive cases was significantly less than that of SWCA-ELISA in both melioidosis and healthy individuals (0.79±0.11 versus 2.4±0.08, p = 0.0001; 0.67±0.01 versus 1.27±0.02, p = 0.0001). The sensitivity and specificity of RTFA-ELISA were 88.9% and 100% respectively. Conclusion: Findings of the study suggest that multiple or combination of antigens should be used to study the seroprevalence of B. pseudomalleiinfection in a community. Also, prospective study is necessary to find out the duration of persistence of antibodies to different antigenic components of B. pseudomallei after exposure. IMC J Med Sci 2020; 14(1): 010. EPub date: 31 May 2020 *Correspondence: J. Ashraful Haq, Department of Microbiology, Ibrahim Medical College, 1/A Ibrahim Sarani, Segunbagicha, Dhaka 1000, ...
Keywords	Medicine ; R
Subject code	630
Language	English
Publishing date	2020-06-01T00:00:00Z
Publisher	Ibrahim Medical College
Document type	Article ; Online
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Article ; Online: Polymorphonuclear neutrophil response to Burkholderia pseudomallei in patients with diabetes mellitus

Sraboni Mazumder / Lovely Barai / Md. Shariful Alam Jilani / K.M. Shahidul Islam / Jalaluddin Ashraful Haq

IMC Journal of Medical Science, Vol 13, Iss 2, Pp 1-

2019 Volume 7

Abstract: Background and objectives: Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic in Bangladesh. Diabetes mellitus (DM) is a risk factor for increased susceptibility to B. pseudomallei infection. A few ... ...

Abstract	Background and objectives: Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic in Bangladesh. Diabetes mellitus (DM) is a risk factor for increased susceptibility to B. pseudomallei infection. A few studies have been conducted to identify the underlying immunological mechanism responsible for increased susceptibility of individuals with diabetes mellitus to B. pseudomallei infection. The present study investigated the polymorphonuclear neutrophil (PMN) response to B. pseudomallei in terms of phagocytosis and early respiratory burst in individuals with diabetes mellitus. Materials and Methods: A total of 5 cases of DM and 5 age and sex matched non-diabetic healthy individuals were enrolled in the study to determine the early respiratory burst and phagocytic ability of PMN to B. pseudomallei. The effect of B. pseudomallei on phagocytic ability and early respiratory burst of PMN was determined by phagocytic assay and nitroblue tetrazolium (NBT) test respectively. The response of PMN treated with B. pseudomallei was compared with that of Escherichia coli. Results: There was no significant (p>0.05) difference in phagocytosis of B. pseudomallei by PMN between diabetic and non-diabetic cases (21.8±4.64 percent vs 29.25±5.5 percent). But in both diabetic and non-diabetic cases, significantly (p˂0.05 and p˂0.01) reduced rate of phagocytosis of B. pseudomallei by PMN was observed compared to E. coli (21.8±4.64 vs 65±5.36; 29.25±5.5 vs 71.25±5.59). Similar results were obtained in terms of phagocytic index. Mean percentage of formazan positive PMN from diabetic cases was not significantly different (p>0.05) from non-diabetic healthy cases when cells were treated with B. pseudomallei or E. coli. In both diabetic and healthy individuals, mean percentage of formazan positive PMN treated by B. pseudomallei was not significantly different from that by E. coli. Conclusion: The observations revealed that B. pseudomallei was equally capable of inhibiting the phagocytic ability ...
Keywords	Medicine ; R
Subject code	571
Language	English
Publishing date	2019-09-01T00:00:00Z
Publisher	Ibrahim Medical College
Document type	Article ; Online
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Article ; Online: Serological diagnosis for active tuberculosis in Malaysian population

Siti Suraiya / Mustaffa Musa / Rapeah Suppian / Jalaluddin Ashraful Haq

Asian Pacific Journal of Tropical Disease, Vol 2, Iss Sup 1, Pp 312-

Comparison of four protein candidate

2012 Volume 315

Abstract: Objective: To asses the ability of 4 types of Mtb proteins-ESAT6, SCWP, MAN and Ag85 to serve as indicator for active tuberculosis among Malaysian population. Methods: Sera from 90 individuals, 60 from confirmed pulmonary tuberculosis patients and 30 ... ...

Abstract	Objective: To asses the ability of 4 types of Mtb proteins-ESAT6, SCWP, MAN and Ag85 to serve as indicator for active tuberculosis among Malaysian population. Methods: Sera from 90 individuals, 60 from confirmed pulmonary tuberculosis patients and 30 healthy PPD negative individuals were tested for presence of anti-IgG and anti IgA by ELISA assay. Result: Mean concentration of IgG and IgA were higher in patients compared to healthy Positivity of the ELISA test were calculated, taking the cut off value at mean +2 SD of healthy sera. The sensitivity of the ELISA IgA assay for ESAT 6, SCWP, MAN and Ag85 were 81.1%, 83.3%, 11.7% and 53.3% respectively. The sensitivity of the ELISA IgG asay for ESAT 6, SCWP, MAN and Ag85 were 71.0%, 71.0%, 71.0% and 21.0% respectively. Conclusion: Detectionof IgA against SCWP promised a good indicator for active tuberculosis infection among Malaysian.
Keywords	SCWP ; Ag85 ; LAM ; ESAT6 ; Serology ; Tuberculosis ; Malaysia ; Medicine ; R ; Arctic medicine. Tropical medicine ; RC955-962
Language	English
Publishing date	2012-05-01T00:00:00Z
Publisher	Asian Pacific Journal of Tropical Disease Editorial Office
Document type	Article ; Online
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