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  1. Article ; Online: Correction of Spacecraft Magnetic Field Noise: Initial Korean Pathfinder Lunar Orbiter MAGnetometer Observation in Solar Wind.

    Lee, Junhyun / Jin, Ho / Kim, Khan-Hyuk / Park, Hyeonhu / Jo, Wooin / Jang, Yunho / Kang, Hyeonji / Kim, Eunhyeuk / Choi, Young-Jun

    Sensors (Basel, Switzerland)

    2023  Volume 23, Issue 23

    Abstract: The Korean Pathfinder Lunar Orbiter (KPLO)-MAGnetometer (KMAG) consists of three triaxial fluxgate sensors (MAG1, MAG2, and MAG3) that measure the magnetic field around the Moon. The three sensors are mounted in the order MAG3, MAG2, and MAG1 inside a 1 ... ...

    Abstract The Korean Pathfinder Lunar Orbiter (KPLO)-MAGnetometer (KMAG) consists of three triaxial fluxgate sensors (MAG1, MAG2, and MAG3) that measure the magnetic field around the Moon. The three sensors are mounted in the order MAG3, MAG2, and MAG1 inside a 1.2 m long boom, away from the satellite body. Before it arrived on the Moon, we compared the magnetic field measurements taken by DSCOVR and KPLO in solar wind to verify the measurement performance of the KMAG instrument. We found that there were artificial disturbances in the KMAG measurement data, such as step-like and spike-like disturbances, which were produced by the spacecraft body. To remove spacecraft-generated disturbances, we applied a multi-sensor method, employing the gradiometer technique and principal component analysis, using KMAG magnetic field data, and confirmed the successful elimination of spacecraft-generated disturbances. In the future, the proposed multi-sensor method is expected to clean the magnetic field data measured onboard the KPLO from the lunar orbit.
    Language English
    Publishing date 2023-11-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s23239428
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Novel influenza A viruses in pigs with zoonotic potential, Chile.

    Tapia, Rodrigo / Brito, Bárbara / Saavedra, Marco / Mena, Juan / García-Salum, Tamara / Rathnasinghe, Raveen / Barriga, Gonzalo / Tapia, Karla / García, Victoria / Bucarey, Sergio / Jang, Yunho / Wentworth, David / Torremorell, Montserrat / Neira, Víctor / Medina, Rafael A

    Microbiology spectrum

    2024  Volume 12, Issue 4, Page(s) e0218123

    Abstract: Novel H1N2 and H3N2 swine influenza A viruses (IAVs) have recently been identified in Chile. The objective of this study was to evaluate their zoonotic potential. We perform phylogenetic analyses to determine the genetic origin and evolution of these ... ...

    Abstract Novel H1N2 and H3N2 swine influenza A viruses (IAVs) have recently been identified in Chile. The objective of this study was to evaluate their zoonotic potential. We perform phylogenetic analyses to determine the genetic origin and evolution of these viruses, and a serological analysis to determine the level of cross-protective antibodies in the human population. Eight genotypes were identified, all with pandemic H1N1 2009-like internal genes. H1N1 and H1N2 were the subtypes more commonly detected. Swine H1N2 and H3N2 IAVs had hemagglutinin and neuraminidase lineages genetically divergent from IAVs reported worldwide, including human vaccine strains. These genes originated from human seasonal viruses were introduced into the swine population since the mid-1980s. Serological data indicate that the general population is susceptible to the H3N2 virus and that elderly and young children also lack protective antibodies against the H1N2 strains, suggesting that these viruses could be potential zoonotic threats. Continuous IAV surveillance and monitoring of the swine and human populations is strongly recommended.IMPORTANCEIn the global context, where swine serve as crucial intermediate hosts for influenza A viruses (IAVs), this study addresses the pressing concern of the zoonotic potential of novel reassortant strains. Conducted on a large scale in Chile, it presents a comprehensive account of swine influenza A virus diversity, covering 93.8% of the country's industrialized swine farms. The findings reveal eight distinct swine IAV genotypes, all carrying a complete internal gene cassette of pandemic H1N1 2009 origin, emphasizing potential increased replication and transmission fitness. Genetic divergence of H1N2 and H3N2 IAVs from globally reported strains raises alarms, with evidence suggesting introductions from human seasonal viruses since the mid-1980s. A detailed serological analysis underscores the zoonotic threat, indicating susceptibility in the general population to swine H3N2 and a lack of protective antibodies in vulnerable demographics. These data highlight the importance of continuous surveillance, providing crucial insights for global health organizations.
    MeSH term(s) Child ; Humans ; Animals ; Swine ; Child, Preschool ; Aged ; Influenza A virus/genetics ; Influenza A Virus, H3N2 Subtype/genetics ; Orthomyxoviridae Infections/epidemiology ; Orthomyxoviridae Infections/veterinary ; Influenza A Virus, H1N1 Subtype/genetics ; Phylogeny ; Chile/epidemiology ; Reassortant Viruses/genetics ; Swine Diseases/epidemiology ; Influenza, Human/epidemiology
    Language English
    Publishing date 2024-03-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.02181-23
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  3. Article ; Online: Pathogenesis and Transmission Assessment of 3 Swine-Origin Influenza A(H3N2) Viruses With Zoonotic Risk to Humans Isolated in the United States, 2017-2020.

    Sun, Xiangjie / Belser, Jessica A / Pulit-Penaloza, Joanna A / Brock, Nicole / Pappas, Claudia / Zanders, Natosha / Jang, Yunho / Jones, Joyce / Tumpey, Terrence M / Davis, C Todd / Maines, Taronna R

    The Journal of infectious diseases

    2023  Volume 229, Issue 4, Page(s) 1107–1111

    Abstract: The sporadic occurrence of human infections with swine-origin influenza A(H3N2) viruses and the continual emergence of novel A(H3N2) viruses in swine herds underscore the necessity for ongoing assessment of the pandemic risk posed by these viruses. Here, ...

    Abstract The sporadic occurrence of human infections with swine-origin influenza A(H3N2) viruses and the continual emergence of novel A(H3N2) viruses in swine herds underscore the necessity for ongoing assessment of the pandemic risk posed by these viruses. Here, we selected 3 recent novel swine-origin A(H3N2) viruses isolated between 2017 to 2020, bearing hemagglutinins from the 1990.1, 2010.1, or 2010.2 clades, and evaluated their ability to cause disease and transmit in a ferret model. We conclude that despite considerable genetic variances, all 3 contemporary swine-origin A(H3N2) viruses displayed a capacity for robust replication in the ferret respiratory tract and were also capable of limited airborne transmission. These findings highlight the continued public health risk of swine-origin A(H3N2) strains, especially in human populations with low cross-reactive immunity.
    MeSH term(s) Humans ; Animals ; United States/epidemiology ; Swine ; Influenza, Human ; Orthomyxoviridae Infections ; Influenza A Virus, H3N2 Subtype/genetics ; Ferrets ; Influenza A virus ; Swine Diseases
    Language English
    Publishing date 2023-08-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiad359
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Highly Pathogenic Avian Influenza A(H5N1) Virus Infection in a Dairy Farm Worker.

    Uyeki, Timothy M / Milton, Scott / Abdul Hamid, Cherissa / Reinoso Webb, Cynthia / Presley, Steven M / Shetty, Varun / Rollo, Susan N / Martinez, Diana L / Rai, Saroj / Gonzales, Emilio R / Kniss, Krista L / Jang, Yunho / Frederick, Julia C / De La Cruz, Juan A / Liddell, Jimma / Di, Han / Kirby, Marie K / Barnes, John R / Davis, C Todd

    The New England journal of medicine

    2024  

    Language English
    Publishing date 2024-05-03
    Publishing country United States
    Document type Letter
    ZDB-ID 207154-x
    ISSN 1533-4406 ; 0028-4793
    ISSN (online) 1533-4406
    ISSN 0028-4793
    DOI 10.1056/NEJMc2405371
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Antiviral susceptibility of clade 2.3.4.4b highly pathogenic avian influenza A(H5N1) viruses isolated from birds and mammals in the United States, 2022.

    Nguyen, Ha T / Chesnokov, Anton / De La Cruz, Juan / Pascua, Philippe Noriel Q / Mishin, Vasiliy P / Jang, Yunho / Jones, Joyce / Di, Han / Ivashchenko, Andrei A / Killian, Mary Lea / Torchetti, Mia K / Lantz, Kristina / Wentworth, David E / Davis, Charles T / Ivachtchenko, Alexandre V / Gubareva, Larisa V

    Antiviral research

    2023  Volume 217, Page(s) 105679

    Abstract: Clade 2.3.4.4b highly pathogenic avian influenza (HPAI) A(H5N1) viruses that are responsible for devastating outbreaks in birds and mammals pose a potential threat to public health. Here, we evaluated their susceptibility to influenza antivirals. Of 1, ... ...

    Abstract Clade 2.3.4.4b highly pathogenic avian influenza (HPAI) A(H5N1) viruses that are responsible for devastating outbreaks in birds and mammals pose a potential threat to public health. Here, we evaluated their susceptibility to influenza antivirals. Of 1,015 sequences of HPAI A(H5N1) viruses collected in the United States during 2022, eight viruses (∼0.8%) had a molecular marker of drug resistance to an FDA-approved antiviral: three adamantane-resistant (M2-V27A), four oseltamivir-resistant (NA-H275Y), and one baloxavir-resistant (PA-I38T). Additionally, 31 viruses contained mutations that may reduce susceptibility to inhibitors of neuraminidase (NA) (n = 20) or cap-dependent endonuclease (CEN) (n = 11). A panel of 22 representative viruses was tested phenotypically. Overall, clade 2.3.4.4b A(H5N1) viruses lacking recognized resistance mutations were susceptible to FDA-approved antivirals. Oseltamivir was least potent at inhibiting NA activity, while the investigational NA inhibitor AV5080 was most potent, including against NA mutants. A novel NA substitution T438N conferred 12-fold reduced inhibition by zanamivir, and in combination with the known marker N295S, synergistically affected susceptibility to all five NA inhibitors. In cell culture-based assays HINT and IRINA, the PA-I38T virus displayed 75- to 108-fold and 37- to 78-fold reduced susceptibility to CEN inhibitors, baloxavir and the investigational AV5116, respectively. Viruses with PA-I38M or PA-A37T showed 5- to 10-fold reduced susceptibilities. As HPAI A(H5N1) viruses continue to circulate and evolve, close monitoring of drug susceptibility is needed for risk assessment and to inform decisions regarding antiviral stockpiling.
    MeSH term(s) Animals ; United States/epidemiology ; Antiviral Agents/pharmacology ; Oseltamivir/pharmacology ; Influenza A Virus, H5N1 Subtype/genetics ; Influenza in Birds/epidemiology ; Enzyme Inhibitors/pharmacology ; Birds ; Mammals ; Drug Resistance, Viral/genetics ; Neuraminidase
    Chemical Substances Antiviral Agents ; Oseltamivir (20O93L6F9H) ; baloxavir (4G86Y4JT3F) ; Enzyme Inhibitors ; Neuraminidase (EC 3.2.1.18)
    Language English
    Publishing date 2023-07-24
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 306628-9
    ISSN 1872-9096 ; 0166-3542
    ISSN (online) 1872-9096
    ISSN 0166-3542
    DOI 10.1016/j.antiviral.2023.105679
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Avian influenza A(H5) virus circulation in live bird markets in Vietnam, 2017-2022.

    Nguyen, Diep T / Sumner, Kelsey M / Nguyen, Thoa T M / Phan, Minh Q / Hoang, Tien M / Vo, Chuong D / Nguyen, Tho D / Nguyen, Phuong T / Yang, Genyan / Jang, Yunho / Jones, Joyce / Olsen, Sonja J / Gould, Philip L / Nguyen, Long V / Davis, Charles Todd

    Influenza and other respiratory viruses

    2023  Volume 17, Issue 12, Page(s) e13245

    Abstract: Background: Highly pathogenic avian influenza A(H5) human infections are a global concern, with many A(H5) human cases detected in Vietnam, including a case in October 2022. Using avian influenza virus surveillance from March 2017-September 2022, we ... ...

    Abstract Background: Highly pathogenic avian influenza A(H5) human infections are a global concern, with many A(H5) human cases detected in Vietnam, including a case in October 2022. Using avian influenza virus surveillance from March 2017-September 2022, we described the percent of pooled samples that were positive for avian influenza A, A(H5), A(H5N1), A(H5N6), and A(H5N8) viruses in live bird markets (LBMs) in Vietnam.
    Methods: Monthly at each LBM, 30 poultry oropharyngeal swab specimens and five environmental samples were collected. Samples were pooled in groups of five and tested for influenza A, A(H5), A(H5N1), A(H5N6), and A(H5N8) viruses by real-time reverse-transcription polymerase chain reaction. Trends in the percent of pooled samples that were positive for avian influenza were summarized by LBM characteristics and time and compared with the number of passively detected avian influenza outbreaks using Spearman's rank correlation.
    Results: A total of 25,774 pooled samples were collected through active surveillance at 167 LBMs in 24 provinces; 36.9% of pooled samples were positive for influenza A, 3.6% A(H5), 1.9% A(H5N1), 1.1% A(H5N6), and 0.2% A(H5N8). Influenza A(H5) viruses were identified January-December and at least once in 91.7% of sampled provinces. In 246 A(H5) outbreaks in poultry; 20.3% were influenza A(H5N1), 60.2% A(H5N6), and 19.5% A(H5N8); outbreaks did not correlate with active surveillance.
    Conclusions: In Vietnam, influenza A(H5) viruses were detected by active surveillance in LBMs year-round and in most provinces sampled. In addition to outbreak reporting, active surveillance for A(H5) viruses in settings with high potential for animal-to-human spillover can provide situational awareness.
    MeSH term(s) Animals ; Humans ; Influenza, Human/epidemiology ; Influenza in Birds/epidemiology ; Vietnam/epidemiology ; Influenza A Virus, H5N1 Subtype/genetics ; Disease Outbreaks ; Influenza A Virus, H5N8 Subtype ; Influenza A virus/genetics
    Language English
    Publishing date 2023-12-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2274538-5
    ISSN 1750-2659 ; 1750-2640
    ISSN (online) 1750-2659
    ISSN 1750-2640
    DOI 10.1111/irv.13245
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  7. Article ; Online: Pathogenesis and transmission of human seasonal and swine-origin A(H1) influenza viruses in the ferret model.

    Pulit-Penaloza, Joanna A / Brock, Nicole / Jones, Joyce / Belser, Jessica A / Jang, Yunho / Sun, Xiangjie / Thor, Sharmi / Pappas, Claudia / Zanders, Natosha / Tumpey, Terrence M / Davis, C Todd / Maines, Taronna R

    Emerging microbes & infections

    2022  Volume 11, Issue 1, Page(s) 1452–1459

    Abstract: Influenza A viruses (IAVs) in the swine reservoir constantly evolve, resulting in expanding genetic and antigenic diversity of strains that occasionally cause infections in humans and pose a threat of emerging as a strain capable of human-to-human ... ...

    Abstract Influenza A viruses (IAVs) in the swine reservoir constantly evolve, resulting in expanding genetic and antigenic diversity of strains that occasionally cause infections in humans and pose a threat of emerging as a strain capable of human-to-human transmission. For these reasons, there is an ongoing need for surveillance and characterization of newly emerging strains to aid pandemic preparedness efforts, particularly for the selection of candidate vaccine viruses and conducting risk assessments. Here, we performed a parallel comparison of the pathogenesis and transmission of genetically and antigenically diverse swine-origin A(H1N1) variant (v) and A(H1N2)v, and human seasonal A(H1N1)pdm09 IAVs using the ferret model. Both groups of viruses were capable of replication in the ferret upper respiratory tract; however, variant viruses were more frequently isolated from the lower respiratory tract as compared to the human-adapted viruses. Regardless of virus origin, observed clinical signs of infection differed greatly between strains, with some viruses causing nasal discharge, sneezing and, in some instances, diarrhea in ferrets. The most striking difference between the viruses was the ability to transmit through the air. Human-adapted viruses were capable of airborne transmission between all ferret pairs. In contrast, only one out of the four tested variant viruses was able to transmit via the air as efficiently as the human-adapted viruses. Overall, this work highlights the need for sustained monitoring of emerging swine IAVs to identify strains of concern such as those that are antigenically different from vaccine strains and that possess adaptations required for efficient respiratory droplet transmission in mammals.
    MeSH term(s) Animals ; Ferrets ; Humans ; Influenza A Virus, H1N1 Subtype/genetics ; Influenza A virus ; Influenza, Human ; Orthomyxoviridae Infections ; Seasons ; Swine
    Language English
    Publishing date 2022-05-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2681359-2
    ISSN 2222-1751 ; 2222-1751
    ISSN (online) 2222-1751
    ISSN 2222-1751
    DOI 10.1080/22221751.2022.2076615
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Multiplex assay for subtyping avian influenza A viruses by cDNA hybridization and adapter-mediated amplification.

    Yang, Genyan / Jones, Joyce / Jang, Yunho / Davis, C Todd

    Applied microbiology and biotechnology

    2016  Volume 100, Issue 20, Page(s) 8809–8818

    Abstract: Multiple subtypes of influenza A viruses circulating in animals must be closely monitored to understand their risk to humans and animal populations. Many molecular-based subtyping methods require constant monitoring of viral genomes for primer and/or ... ...

    Abstract Multiple subtypes of influenza A viruses circulating in animals must be closely monitored to understand their risk to humans and animal populations. Many molecular-based subtyping methods require constant monitoring of viral genomes for primer and/or probe mismatches and are prone to primer-primer interactions. This report presents a new approach that involves target enrichment through cDNA hybridization followed by adapter-mediated amplification for subtyping influenza virus (AmASIV). As a proof of concept, the AmASIV assay was multiplexed to specifically detect and differentiate influenza A virus subtypes (H5, N5, N7, and N9) in a single reaction without cross-recognition of nontarget subtypes or influenza B virus. The limit of detection (LOD) of AmASIV, as measured by 50 % egg-infective dose per reaction (EID50/reaction), was comparable to that of singleplex TaqMan® qPCR assays with LODs of 10(-0.6) (H5), 10(2) (N5), 10(-0.3) (N7), and 10(-0.5) (N9) EID50/reaction. The AmASIV will strengthen animal influenza virus surveillance and laboratory capacity to improve prevention and control of influenza.
    MeSH term(s) Animals ; Birds ; Genotyping Techniques/methods ; Influenza A virus/classification ; Influenza A virus/genetics ; Influenza in Birds/virology ; Nucleic Acid Amplification Techniques/methods ; Nucleic Acid Hybridization/methods ; Sensitivity and Specificity
    Language English
    Publishing date 2016-10
    Publishing country Germany
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-016-7664-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Development of Chemical Bond based Elastic Network Model and its application in identifying functional motions in H5N1 highly pathogenic avian influenza viruses.

    Jang, Yunho / Wan, Xiu-Feng

    International journal of bioinformatics research and applications

    2009  Volume 6, Issue 1, Page(s) 1–11

    Abstract: Here we proposed a Chemical Bond based Elastic Network Model (CB-ENM) method, which was demonstrated with a better performance in representing functional movements, especially the local motions, than the conventional normal mode analysis. CB-ENM was ... ...

    Abstract Here we proposed a Chemical Bond based Elastic Network Model (CB-ENM) method, which was demonstrated with a better performance in representing functional movements, especially the local motions, than the conventional normal mode analysis. CB-ENM was applied in characterising functional motions in surface glycoprotein Haemagglutinin (HA) of H5N1 highly pathogenic avian influenza virus. Eleven local peaks were identified amongst HA fluctuation spectrum, and eight of these peaks were correlated with the reported five antigenic binding sites and Receptor Binding Sites (RBS) in influenza viruses. Our study showed that CB-ENM has a potential in identifying functional residues in protein structure.
    MeSH term(s) Antigens, Viral/chemistry ; Binding Sites ; Hemagglutinin Glycoproteins, Influenza Virus/chemistry ; Influenza A Virus, H5N1 Subtype/chemistry ; Influenza A Virus, H5N1 Subtype/genetics ; Influenza A Virus, H5N1 Subtype/pathogenicity ; Models, Biological ; Models, Molecular ; Neural Networks (Computer) ; Protein Conformation ; Viral Core Proteins/chemistry
    Chemical Substances Antigens, Viral ; Hemagglutinin Glycoproteins, Influenza Virus ; Viral Core Proteins
    Language English
    Publishing date 2009-12-02
    Publishing country Switzerland
    Document type Journal Article
    ISSN 1744-5485
    ISSN 1744-5485
    DOI 10.1504/IJBRA.2010.031288
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  10. Article: A nationwide survey on the management of the COVID-19 pandemic and respiratory disease in South Korea.

    Ang, Lin / Yim, Mi Hong / Song, Eunhye / Lee, Hye Won / Lee, Hyangsook / Kim, Tae-Hun / Willcox, Merlin / Hu, Xiao-Yang / Houriet, Joelle / Graz, Bertrand / Lee, Je-Won / Jang, Yunho / Kim, Jung Tae / Kim, Eunsop / Park, Yong Hee / Lee, Myeong Soo

    Frontiers in medicine

    2022  Volume 9, Page(s) 965651

    Abstract: Background: This study aimed to explore individual prevalence of respiratory symptoms and to describe the Korean population's treatment approaches, preventive health behaviors, and mental health conditions during the pandemic.: Methods: We analyzed ... ...

    Abstract Background: This study aimed to explore individual prevalence of respiratory symptoms and to describe the Korean population's treatment approaches, preventive health behaviors, and mental health conditions during the pandemic.
    Methods: We analyzed responses from an online nationwide survey, conducted between February 2021 to May 2021, about people's experiences during the pandemic. Statistical analysis was also performed to see if there were any significant differences in treatment and prevention strategies between different groups of respondents (between those had respiratory symptoms, compared with those who did not, and between those tested positive for COVID-19, compared with those who did not).
    Results: A total of 2,177 survey respondents completed the survey and, of these, only 142 had experienced symptoms. The most frequently reported respiratory infections related symptoms were runny or blocked nose (47.6%), cough (45.5%), fever (44.1%), sore throat (42.0%), and fatigue (30.1%). More than half of the respondents (53.1%) used complementary and alternative medicine (CAM) approaches as means of preventive measures. In terms of preventive behaviors, the more emphasized behaviors were mask-wearing (58.9%) and hand-washing after coming home (42.7%). The majority of the respondents (64.9%) did not show signs of mental health issues.
    Conclusion: In South Korea, conventional medicine was mainly used for COVID-19 treatment whereas CAM was commonly used as preventive measures. COVID-19 was also found to have less impact on the general population's mental health. The findings of this study may shed light on how the pandemic impacted the general population.
    Language English
    Publishing date 2022-09-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2022.965651
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