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  1. AU="Jay D Evans"
  2. AU=Unger Jean-Pierre
  3. AU="Soday, Lior"
  4. AU="Wan, Xuan"
  5. AU="Camille Fritzell"
  6. AU=Wei Huijun
  7. AU="Levine, Morgan E"
  8. AU="Chen, Yalei"
  9. AU="Rogaeva, Ekaterina" AU="Rogaeva, Ekaterina"
  10. AU="Jain, Ishaan"
  11. AU="Chatelier, Josh"
  12. AU="Passarelli, L."
  13. AU="Marques, R"
  14. AU="Restaino, Valeria"
  15. AU="Wang, Haochen"
  16. AU=Shoib Sheikh
  17. AU=Patel Ishan
  18. AU="Mongioì, Laura M"
  19. AU="Fernández-Pacheco, Borja Camacho"
  20. AU=Waghmare Alpana AU=Waghmare Alpana
  21. AU="Peyre, Marion"
  22. AU=Mulazimoglu L
  23. AU=Roy Satyaki
  24. AU="Li Yuanyuan"
  25. AU=Khan Shehryar
  26. AU=Cole Sarah L
  27. AU="Júnior, Raimundo Nonato Colares Camargo"
  28. AU="Feeney, Judith A"

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  1. Artikel ; Online: Can floral nectars reduce transmission of Leishmania?

    Evan C. Palmer-Young / Ryan S. Schwarz / Yanping Chen / Jay D. Evans

    PLoS Neglected Tropical Diseases, Vol 16, Iss

    2022  Band 5

    Abstract: Background Insect-vectored Leishmania are responsible for loss of more disability-adjusted life years than any parasite besides malaria. Elucidation of the environmental factors that affect parasite transmission by vectors is essential to develop ... ...

    Abstract Background Insect-vectored Leishmania are responsible for loss of more disability-adjusted life years than any parasite besides malaria. Elucidation of the environmental factors that affect parasite transmission by vectors is essential to develop sustainable methods of parasite control that do not have off-target effects on beneficial insects or environmental health. Many phytochemicals that inhibit growth of sand fly-vectored Leishmania—which have been exhaustively studied in the search for phytochemical-based drugs—are abundant in nectars, which provide sugar-based meals to infected sand flies. Principle findings In a quantitative meta-analysis, we compare inhibitory phytochemical concentrations for Leishmania to concentrations present in floral nectar and pollen. We show that nectar concentrations of several flowering plant species exceed those that inhibit growth of Leishmania cell cultures, suggesting an unexplored, landscape ecology-based approach to reduce Leishmania transmission. Significance If nectar compounds are as effective against parasites in the sand fly gut as predicted from experiments in vitro, strategic planting of antiparasitic phytochemical-rich floral resources or phytochemically enriched baits could reduce Leishmania loads in vectors. Such interventions could provide an environmentally friendly complement to existing means of disease control. Author summary Leishmania parasites infect over a million people each year—including over 200,000 infections with deadly visceral leishmaniasis—resulting in a greater health burden than any human parasite besides malaria. Leishmania infections of humans are transmitted by blood-feeding sand flies, which also consume floral nectar. Nectar contains many chemicals that inhibit Leishmania growth and are candidate treatments for infection of humans. However, these same compounds could also reduce infection in nectar-consuming sand flies. By combining existing data on the chemistry of nectar and sensitivity of Leishmania to plant compounds, we show that ...
    Schlagwörter Arctic medicine. Tropical medicine ; RC955-962 ; Public aspects of medicine ; RA1-1270
    Thema/Rubrik (Code) 572
    Sprache Englisch
    Erscheinungsdatum 2022-05-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Promiscuous feeding on multiple adult honey bee hosts amplifies the vectorial capacity of Varroa destructor.

    Zachary S Lamas / Serhat Solmaz / Eugene V Ryabov / Joseph Mowery / Matthew Heermann / Daniel Sonenshine / Jay D Evans / David J Hawthorne

    PLoS Pathogens, Vol 19, Iss 1, p e

    2023  Band 1011061

    Abstract: Varroa destructor is a cosmopolitan pest and leading cause of colony loss of the European honey bee. Historically described as a competent vector of honey bee viruses, this arthropod vector is the cause of a global pandemic of Deformed wing virus, now ... ...

    Abstract Varroa destructor is a cosmopolitan pest and leading cause of colony loss of the European honey bee. Historically described as a competent vector of honey bee viruses, this arthropod vector is the cause of a global pandemic of Deformed wing virus, now endemic in honeybee populations in all Varroa-infested regions. Our work shows that viral spread is driven by Varroa actively switching from one adult bee to another as they feed. Assays using fluorescent microspheres were used to indicate the movement of fluids in both directions between host and vector when Varroa feed. Therefore, Varroa could be in either an infectious or naïve state dependent upon the disease status of their host. We tested this and confirmed that the relative risk of a Varroa feeding depended on their previous host's infectiousness. Varroa exhibit remarkable heterogeneity in their host-switching behavior, with some Varroa infrequently switching while others switch at least daily. As a result, relatively few of the most active Varroa parasitize the majority of bees. This multiple-feeding behavior has analogs in vectorial capacity models of other systems, where promiscuous feeding by individual vectors is a leading driver of vectorial capacity. We propose that the honeybee-Varroa relationship offers a unique opportunity to apply principles of vectorial capacity to a social organism, as virus transmission is both vectored and occurs through multiple host-to-host routes common to a crowded society.
    Schlagwörter Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2023-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Comparative genomics suggests local adaptations in the invasive small hive beetle

    Yuanzhen Liu / Jan Henkel / Alexis Beaurepaire / Jay D. Evans / Peter Neumann / Qiang Huang

    Ecology and Evolution, Vol 11, Iss 22, Pp 15780-

    2021  Band 15791

    Abstract: Abstract Invasive species are a major driver of ecological and environmental changes that affect human health, food security, and natural biodiversity. The success and impact of biological invasions depend on adaptations to novel abiotic and biotic ... ...

    Abstract Abstract Invasive species are a major driver of ecological and environmental changes that affect human health, food security, and natural biodiversity. The success and impact of biological invasions depend on adaptations to novel abiotic and biotic selective pressures. However, the molecular mechanisms underlying adaptations in invasive parasitic species are inadequately understood. Small hive beetles, Aethina tumida, are parasites of bee nests. Originally endemic to sub‐Saharan Africa, they are now found nearly globally. Here, we investigated the molecular bases of the adaptations to novel environments underlying their invasion routes. Genomes of historic and recent adults A. tumida from both the endemic and introduced ranges were compared. Analysis of gene–environment association identified 3049 candidate loci located in 874 genes. Functional annotation showed a significant bias toward genes linked to growth and reproduction. One of the genes from the apoptosis pathway encodes an “ecdysone‐related protein,” which is a crucial regulator in controlling body size in response to environmental cues for holometabolous insects during cell death and renewal. Genes whose proteins regulate organ size, ovary activation, and oviposition were also detected. Functions of these enriched pathways parallel behavioral differences between introduced and native A. tumida populations, which may reflect patterns of local adaptation. The results considerably improve our understanding of the underlying mechanisms and ecological factors driving adaptations of invasive species. Deep functional investigation of these identified loci will help clarify the mechanisms of local adaptation in A. tumida.
    Schlagwörter invasive species ; molecular evolution ; natural selection ; Ecology ; QH540-549.5
    Thema/Rubrik (Code) 580
    Sprache Englisch
    Erscheinungsdatum 2021-11-01T00:00:00Z
    Verlag Wiley
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Host-driven temperature dependence of Deformed wing virus infection in honey bee pupae

    Evan C. Palmer-Young / Eugene V. Ryabov / Lindsey M. Markowitz / Dawn L. Boncristiani / Kyle Grubbs / Asha Pawar / Raymond Peterson / Jay D. Evans

    Communications Biology, Vol 6, Iss 1, Pp 1-

    2023  Band 7

    Abstract: Deformed Wing Virus best infects honey bee pupae at temperatures optimal for pupal development—not for viral enzymes—suggesting host facilitation of virus replication, and tradeoffs between infection resistance and bee survival. ...

    Abstract Deformed Wing Virus best infects honey bee pupae at temperatures optimal for pupal development—not for viral enzymes—suggesting host facilitation of virus replication, and tradeoffs between infection resistance and bee survival.
    Schlagwörter Biology (General) ; QH301-705.5
    Sprache Englisch
    Erscheinungsdatum 2023-03-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel: Identification of microRNA-like small RNAs from fungal parasite Nosema ceranae

    Huang, Qiang / Jay D. Evans

    Journal of invertebrate pathology. 2016 Jan., v. 133

    2016  

    Abstract: We previously found transcripts encoding Dicer and Argonaute which are involved in the production of microRNAs, in the honey bee parasite Nosema ceranae. In order to identify microRNAs in N. ceranae, we sequenced small RNAs from midgut tissues of ... ...

    Abstract We previously found transcripts encoding Dicer and Argonaute which are involved in the production of microRNAs, in the honey bee parasite Nosema ceranae. In order to identify microRNAs in N. ceranae, we sequenced small RNAs from midgut tissues of infected honey bees at 24h intervals for 6days post infection, covering the complete reproduction cycle for this intracellular parasite. We predicted six microRNA-like small RNAs, all of which were confirmed via RT-qPCR assays. This is the first evidence for microRNA-like small RNAs generated by a microsporidian species, providing new insights into host–parasite interactions involving this widespread taxonomic group.
    Schlagwörter fungi ; honey bees ; host-parasite relationships ; microRNA ; microsporidiosis ; midgut ; Nosema ceranae ; parasites ; quantitative polymerase chain reaction ; reproduction ; reverse transcriptase polymerase chain reaction
    Sprache Englisch
    Erscheinungsverlauf 2016-01
    Umfang p. 107-109.
    Erscheinungsort Elsevier Inc.
    Dokumenttyp Artikel
    ZDB-ID 390885-9
    ISSN 1096-0805 ; 0022-2011
    ISSN (online) 1096-0805
    ISSN 0022-2011
    DOI 10.1016/j.jip.2015.12.005
    Datenquelle NAL Katalog (AGRICOLA)

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  6. Artikel ; Online: Pupal cannibalism by worker honey bees contributes to the spread of deformed wing virus

    Francisco Posada-Florez / Zachary S. Lamas / David J. Hawthorne / Yanping Chen / Jay D. Evans / Eugene V. Ryabov

    Scientific Reports, Vol 11, Iss 1, Pp 1-

    2021  Band 12

    Abstract: Abstract Transmission routes impact pathogen virulence and genetics, therefore comprehensive knowledge of these routes and their contribution to pathogen circulation is essential for understanding host–pathogen interactions and designing control ... ...

    Abstract Abstract Transmission routes impact pathogen virulence and genetics, therefore comprehensive knowledge of these routes and their contribution to pathogen circulation is essential for understanding host–pathogen interactions and designing control strategies. Deformed wing virus (DWV), a principal viral pathogen of honey bees associated with increased honey bee mortality and colony losses, became highly virulent with the spread of its vector, the ectoparasitic mite Varroa destructor. Reproduction of Varroa mites occurs in capped brood cells and mite-infested pupae from these cells usually have high levels of DWV. The removal of mite-infested pupae by worker bees, Varroa Sensitive Hygiene (VSH), leads to cannibalization of pupae with high DWV loads, thereby offering an alternative route for virus transmission. We used genetically tagged DWV to investigate virus transmission to and between worker bees following pupal cannibalisation under experimental conditions. We demonstrated that cannibalization of DWV-infected pupae resulted in high levels of this virus in worker bees and that the acquired virus was then transmitted between bees via trophallaxis, allowing circulation of Varroa-vectored DWV variants without the mites. Despite the known benefits of hygienic behaviour, it is possible that higher levels of VSH activity may result in increased transmission of DWV via cannibalism and trophallaxis.
    Schlagwörter Medicine ; R ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2021-04-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: Multilocus sequence typing, biochemical and antibiotic resistance characterizations reveal diversity of North American strains of the honey bee pathogen Paenibacillus larvae.

    Sasiprapa Krongdang / Jay D Evans / Jeffery S Pettis / Panuwan Chantawannakul

    PLoS ONE, Vol 12, Iss 5, p e

    2017  Band 0176831

    Abstract: Paenibacillus larvae is a Gram positive bacterium and the causative agent of the most widespread fatal brood disease of honey bees, American foulbrood (AFB). A total of thirty-three independent Paenibacillus larvae isolates from various geographical ... ...

    Abstract Paenibacillus larvae is a Gram positive bacterium and the causative agent of the most widespread fatal brood disease of honey bees, American foulbrood (AFB). A total of thirty-three independent Paenibacillus larvae isolates from various geographical origins in North America and five reference strains were investigated for genetic diversity using multilocus sequence typing (MLST). This technique is regarded to be a powerful tool for epidemiological studies of pathogenic bacteria and is widely used in genotyping assays. For MLST, seven housekeeping gene loci, ilvD (dihydroxy-acid dyhydrogenase), tri (triosephosphate isomerase), purH (phospharibosyl-aminoimidazolecarboxamide), recF (DNA replication and repair protein), pyrE (orotate phosphoribosyltransferase), sucC (succinyl coenzyme A synthetase β subunit) and glpF (glycerol uptake facilitator protein) were studied and applied for primer designs. Previously, ERIC type DNA fingerprinting was applied to these same isolates and the data showed that almost all represented the ERIC I type, whereas using BOX-PCR gave an indication of more diversity. All isolates were screened for resistance to four antibiotics used by U.S. beekeepers, showing extensive resistance to tetracycline and the first records of resistance to tylosin and lincomycin. Our data highlight the intraspecies relationships of P. larvae and the potential application of MLST methods in enhancing our understanding of epidemiological relationships among bacterial isolates of different origins.
    Schlagwörter Medicine ; R ; Science ; Q
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2017-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: The Movement of Western Honey Bees (Apis mellifera L.) Among U.S. States and Territories

    Jose Marcelino / Charles Braese / Krisztina Christmon / Jay D. Evans / Todd Gilligan / Tugrul Giray / Anthony Nearman / Elina L. Niño / Robyn Rose / Walter S. Sheppard / Dennis vanEngelsdorp / James D. Ellis

    Frontiers in Ecology and Evolution, Vol

    History, Benefits, Risks, and Mitigation Strategies

    2022  Band 10

    Abstract: Beekeeping is a cornerstone activity that has led to the human-mediated, global spread of western honey bees (Apis mellifera L.) outside their native range of Europe, western Asia, and Africa. The exportation/importation of honey bees (i.e., transfer of ... ...

    Abstract Beekeeping is a cornerstone activity that has led to the human-mediated, global spread of western honey bees (Apis mellifera L.) outside their native range of Europe, western Asia, and Africa. The exportation/importation of honey bees (i.e., transfer of honey bees or germplasm between countries) is regulated at the national level in many countries. Honey bees were first imported into the United States in the early 1600’s. Today, honey bee movement (i.e., transport of honey bees among states and territories) is regulated within the United States at the state, territory, and federal levels. At the federal level, honey bees present in the country (in any state or territory) can be moved among states and territories without federal restriction, with the exception of movement to Hawaii. In contrast, regulations at the state and territory levels vary substantially, ranging from no additional regulations beyond those stipulated at the federal level, to strict regulations for the introduction of live colonies, packaged bees, or queens. This variability can lead to inconsistencies in the application of regulations regarding the movement of honey bees among states and territories. In November 2020, we convened a technical working group (TWG), composed of academic and USDA personnel, to review and summarize the (1) history of honey bee importation into/movement within the United States, (2) current regulations regarding honey bee movement and case studies on the application of those regulations, (3) benefits associated with moving honey bees within the United States, (4) risks associated with moving honey bees within the United States, and (5) risk mitigation strategies. This review will be helpful for developing standardized best practices for the safe movement of honey bees between the 48 contiguous states and other states/territories within the United States.
    Schlagwörter honey bee stock ; Apis mellifera ; human mediated movement ; regulations ; benefits ; risks ; Evolution ; QH359-425 ; Ecology ; QH540-549.5
    Thema/Rubrik (Code) 320
    Sprache Englisch
    Erscheinungsdatum 2022-05-01T00:00:00Z
    Verlag Frontiers Media S.A.
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  9. Artikel ; Online: Varroa destructor mites vector and transmit pathogenic honey bee viruses acquired from an artificial diet.

    Francisco Posada-Florez / Eugene V Ryabov / Matthew C Heerman / Yanping Chen / Jay D Evans / Daniel E Sonenshine / Steven C Cook

    PLoS ONE, Vol 15, Iss 11, p e

    2020  Band 0242688

    Abstract: The ectoparasitic mite Varroa destructor is one of the most destructive pests of the honey bee (Apis mellifera) and the primary biotic cause of colony collapse in many regions of the world. These mites inflict physical injury on their honey bee hosts ... ...

    Abstract The ectoparasitic mite Varroa destructor is one of the most destructive pests of the honey bee (Apis mellifera) and the primary biotic cause of colony collapse in many regions of the world. These mites inflict physical injury on their honey bee hosts from feeding on host hemolymph and fat body cells/cellular components, and serve as the vector for deadly honey bee viruses, including Deformed wing virus (DWV) and the related Varroa destructor virus-1 (VDV-1) (i.e., DWV-like viruses). Studies focused on elucidating the dynamics of Varroa-mediated vectoring and transmission of DWV-like viruses may be confounded by viruses present in ingested host tissues or the mites themselves. Here we describe a system that includes an artificial diet free of insect tissue-derived components for maintaining Varroa mites for in vitro experimentation. Using this system, together with the novel engineered cDNA clone-derived genetically tagged VDV-1 and wild-type DWV, we demonstrated for the first time that Varroa mites provided an artificial diet supplemented with engineered viruses for 36 hours could acquire and transmit sufficient numbers of virus particles to establish an infection in virus-naïve hosts. While the in vitro system described herein provides for only up to five days of mite survival, precluding study of the long-term impacts of viruses on mite health, the system allows for extensive insights into the dynamics of Varroa-mediated vectoring and transmission of honey bee viruses.
    Schlagwörter Medicine ; R ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2020-01-01T00:00:00Z
    Verlag Public Library of Science (PLoS)
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  10. Artikel ; Online: Colony-Level Effects of Amygdalin on Honeybees and Their Microbes

    James P. Tauber / Cansu Ö. Tozkar / Ryan S. Schwarz / Dawn Lopez / Rebecca E. Irwin / Lynn S. Adler / Jay D. Evans

    Insects, Vol 11, Iss 783, p

    2020  Band 783

    Abstract: Amygdalin , a cyanogenic glycoside, is found in the nectar and pollen of almond trees, as well as in a variety of other crops, such as cherries, nectarines, apples and others. It is inevitable that western honeybees ( Apis mellifera ) consistently ... ...

    Abstract Amygdalin , a cyanogenic glycoside, is found in the nectar and pollen of almond trees, as well as in a variety of other crops, such as cherries, nectarines, apples and others. It is inevitable that western honeybees ( Apis mellifera ) consistently consume amygdalin during almond pollination season because almond crops are almost exclusively pollinated by honeybees. This study tests the effects of a field-relevant concentration of amygdalin on honeybee microbes and the activities of key honeybee genes. We executed a two-month field trial providing sucrose solutions with or without amygdalin ad libitum to free-flying honeybee colonies. We collected adult worker bees at four time points and used RNA sequencing technology and our HoloBee database to assess global changes in microbes and honeybee transcripts. Our hypothesis was that amygdalin will negatively affect bee microbes and possibly immune gene regulation. Using a log 2 fold-change cutoff at two and intraday comparisons, we show no large change of bacterial counts, fungal counts or key bee immune gene transcripts, due to amygdalin treatment in relation to the control. However, relatively large titer decreases in the amygdalin treatment relative to the control were found for several viruses. Chronic bee paralysis virus levels had a sharp decrease (−14.4) with titers then remaining less than the control, Black queen cell virus titers were lower at three time points (<−2) and Deformed wing virus titers were lower at two time points (<−6) in amygdalin-fed compared to sucrose-fed colonies. Titers of Lotmaria passim were lower in the treatment group at three of the four dates (<−4). In contrast, Sacbrood virus had two dates with relative increases in its titers (>2). Overall, viral titers appeared to fluctuate more so than bacteria, as observed by highly inconstant patterns between treatment and control and throughout the season. Our results suggest that amygdalin consumption may reduce several honeybee viruses without affecting other microbes or ...
    Schlagwörter honeybees ; natural products ; amygdalin ; microbes ; viruses ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2020-11-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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