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  1. Article ; Online: Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.

    Chaudhary, Divya / Jeena, Anand Singh / Rohit / Gaur, Sonali / Raj, Rishi / Mishra, Shefali / Kajal / Gupta, Om Prakash / Meena, Mintu Ram

    Applied biochemistry and biotechnology

    2024  

    Abstract: RNA interference (RNAi) is a conserved molecular mechanism that plays a critical role in post-transcriptional gene silencing across diverse organisms. This review delves into the role of RNAi in plant functional genomics and its applications in crop ... ...

    Abstract RNA interference (RNAi) is a conserved molecular mechanism that plays a critical role in post-transcriptional gene silencing across diverse organisms. This review delves into the role of RNAi in plant functional genomics and its applications in crop improvement, highlighting its mechanistic insights and practical implications. The review begins with the foundational discovery of RNAi's mechanism, tracing its origins from petunias to its widespread presence in various organisms. Various classes of regulatory non-coding small RNAs, including siRNAs, miRNAs, and phasiRNAs, have been uncovered, expanding the scope of RNAi-mediated gene regulation beyond conventional understanding. These RNA classes participate in intricate post-transcriptional and epigenetic processes that influence gene expression. In the context of crop enhancement, RNAi has emerged as a powerful tool for understanding gene functions. It has proven effective in deciphering gene roles related to stress resistance, metabolic pathways, and more. Additionally, RNAi-based approaches hold promise for integrated pest management and sustainable agriculture, contributing to global efforts in food security. This review discusses RNAi's diverse applications, such as modifying plant architecture, extending shelf life, and enhancing nutritional content in crops. The challenges and future prospects of RNAi technology, including delivery methods and biosafety concerns, are also explored. The global landscape of RNAi research is highlighted, with significant contributions from regions such as China, Europe, and North America. In conclusion, RNAi remains a versatile and pivotal tool in modern plant research, offering novel avenues for understanding gene functions and improving crop traits. Its integration with other biotechnological approaches such as gene editing holds the potential to shape the future of agriculture and sustainable food production.
    Language English
    Publishing date 2024-01-04
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 392344-7
    ISSN 1559-0291 ; 0273-2289
    ISSN (online) 1559-0291
    ISSN 0273-2289
    DOI 10.1007/s12010-023-04850-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Identification of QTL for Banded Leaf and Sheath Blight in Teosinte-Derived Maize Population

    Adhikari, Sneha / Joshi, Anjali / Kumar, Amarjeet / Singh, Narendra Kumar / Jaiswal, Jai Prakash / Jeena, Anand Singh / Singh, Rajesh Pratap / Pandey, Dinesh

    Agricultural research. 2022 June, v. 11, no. 2

    2022  

    Abstract: Banded leaf and sheath blight is considered as the most important disease of maize, and development of resistant varieties is the most viable option. Total one seventy-one [169 BC₁F₅ teosinte-derived maize lines along with teosinte (resistant) and DI-103 ...

    Abstract Banded leaf and sheath blight is considered as the most important disease of maize, and development of resistant varieties is the most viable option. Total one seventy-one [169 BC₁F₅ teosinte-derived maize lines along with teosinte (resistant) and DI-103 (susceptible) as a check] were planted in randomized complete block design in two different dates of sowing (DE₁ and DE₂) under artificially inoculated environments. Molecular profiling of population was carried out with identified 76 polymorphic microsatellite markers. Association of the markers with the quantitative trait loci (QTL) was performed by single marker analysis (SMA) using linear regression and maximum likelihood analysis. The single marker analysis of variance (ANOVA) revealed one major QTL on chromosome 5 and four minor QTLs on chromosomes 1, 3, 4, and 8, respectively, for BLSB resistance under first sowing (DE₁). In second sowing (DE₂), four minor QTLs were detected on four chromosomes 1, 3, 5 and 8. Out of these, nine QTLs two were identified across the environments, which were linked with markers umc1500 and phi10918, considered as stable QTLs. Among seven detected QTLs for BLSB resistance, a major QTL linked with phi10918 was co-localized with three minor QTLs for ear length, kernel rows per ear and kernels per row. These results suggest that the morphological traits and QTLs which have been found to associate with banded leaf and sheath blight resistance are a good choice to enhance BLSB resistance. Utilization of QTLs governing morphological traits like days to anthesis, days to silking, flag leaf architecture, plant height, etc., could be a good choice along with QTLs governing BLSB resistance to enhance BLSB resistance through QTL pyramiding.
    Keywords agricultural research ; chromosomes ; corn ; flowering ; leaf blight ; leaves ; microsatellite repeats ; plant height ; probability analysis ; quantitative traits ; regression analysis
    Language English
    Dates of publication 2022-06
    Size p. 155-163.
    Publishing place Springer India
    Document type Article
    ZDB-ID 2668414-7
    ISSN 2249-7218 ; 2249-720X
    ISSN (online) 2249-7218
    ISSN 2249-720X
    DOI 10.1007/s40003-021-00567-7
    Database NAL-Catalogue (AGRICOLA)

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