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  1. Book ; Online ; Thesis: Biological control of wireworms (Agriotes spp.) in potato cultivation using the entomopathogenic fungus Metarhizium brunneum: Factors that influence the effectiveness of mycoinsecticide formulations

    Paluch, Maximilian / Jürgens, Andreas / Jehle, Johannes / Lehmhus, Jörn

    (Dissertationen aus dem Julius Kühn-Institut)

    2022  

    Institution Technische Universität Darmstadt
    Author's details Maximilian Paluch ; Gutachter: Andreas Jürgens, Johannes Jehle, Jörn Lehmhus ; Technische Universität Darmstadt
    Series title Dissertationen aus dem Julius Kühn-Institut
    Subject code 630
    Language English
    Size 1 Online-Ressource (140 Seiten)
    Publisher Julius Kühn-Institut
    Publishing place Quedlinburg
    Publishing country Germany
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Dissertation, Technische Universität Darmstadt, 2021
    Note Open Access
    Remark Elektronischer Volltext
    HBZ-ID HT021404396
    ISBN 978-3-95547-109-5 ; 3-95547-109-8
    DOI 10.5073/20220425-150427
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Book ; Conference proceedings: Preceedings of the Meeting "Biological Control - its unique role in organic and integrated production"

    Jehle, Johannes A.

    at Zagreb (Croatia), 16 - 20 June, 2013

    (IOBC WPRS bulletin ; 90)

    2013  

    Title variant Insect pathogens and entomoparasitic nematodes ; Proceedings of the Meeting "Biological Control - its unique role in organic and integrated production"
    Institution International Organization for Biological Control of Noxious Animals and Plants / Working Group Insect Pathogens and Entomoparasitic Nematodes
    Event/congress Meeting Biological Control: Its Unique Role in Organic and Integrated Production (2013, Zagreb)
    Author's details IOBC-WPRS Working Group "Insect Pathogens and Entomoparasitic Nematodes". Ed. by: Johannes A. Jehle
    Series title IOBC WPRS bulletin ; 90
    IOBC WPRS bulletin / International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Plalaearctic Regional Section, IOBC WPRS. International Union of Biological Sciences
    Collection IOBC WPRS bulletin / International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Plalaearctic Regional Section, IOBC WPRS. International Union of Biological Sciences
    Language English
    Size XXIV, 364 S. : Ill., graph. Darst.
    Publisher INRA, Unité de Pathologie Végétale
    Publishing place Montfavet
    Publishing country France
    Document type Book ; Conference proceedings
    HBZ-ID HT017753327
    ISBN 978-92-9067-268-5 ; 92-9067-268-4
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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  3. Article ; Online: A practical guide and Galaxy workflow to avoid inter-plasmidic repeat collapse and false gene loss in Unicycler's hybrid assemblies.

    Schäfer, Lea / Jehle, Johannes A / Kleespies, Regina G / Wennmann, Jörg T

    Microbial genomics

    2024  Volume 10, Issue 1

    Abstract: Generating complete, high-quality genome assemblies is key for any downstream analysis, such as comparative genomics. For bacterial genome assembly, various algorithms and fully automated pipelines exist, which are free-of-charge and easily accessible. ... ...

    Abstract Generating complete, high-quality genome assemblies is key for any downstream analysis, such as comparative genomics. For bacterial genome assembly, various algorithms and fully automated pipelines exist, which are free-of-charge and easily accessible. However, these assembly tools often cannot unambiguously resolve a bacterial genome, for example due to the presence of sequence repeat structures on the chromosome or on plasmids. Then, a more sophisticated approach and/or manual curation is needed. Such modifications can be challenging, especially for non-bioinformaticians, because they are generally not considered as a straightforward process. In this study, we propose a standardized approach for manual genome completion focusing on the popular hybrid assembly pipeline Unicycler. The provided Galaxy workflow addresses two weaknesses in Unicycler's hybrid assemblies: (i) collapse of inter-plasmidic repeats and (ii) false loss of single-copy sequences. To demonstrate and validate how to detect and resolve these assembly errors, we use two genomes from the
    MeSH term(s) Workflow ; Plasmids/genetics ; Genome, Bacterial ; Algorithms ; Genomics
    Language English
    Publishing date 2024-01-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835258-0
    ISSN 2057-5858 ; 2057-5858
    ISSN (online) 2057-5858
    ISSN 2057-5858
    DOI 10.1099/mgen.0.001173
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book: Mathematische Modellierung der Populationsdynamik von genetisch veränderten Mikroorganismen am Beispiel von Baculoviren

    Steineke, Susanne / Jehle, Johannes A.

    Forschungsbericht 29889418

    (Texte / Umweltbundesamt ; [20]02,63 ; Umweltforschungsplan des Bundesministeriums für Umwelt, Naturschutz und Reaktorsicherheit)

    2002  

    Author's details von Susanne Steineke und Johannes A. Jehle. In Zusammenarbeit mit Eva Fritsch ... Umweltbundesamt. [Red.: Fachgebiet IV 2.5]
    Series title Texte / Umweltbundesamt ; [20]02,63
    Umweltforschungsplan des Bundesministeriums für Umwelt, Naturschutz und Reaktorsicherheit
    Collection
    Language German
    Size 157 S., Ill., graph. Darst., 30 cm
    Publisher Umweltbundesamt
    Publishing place Berlin
    Publishing country Germany
    Document type Book
    Note Literaturverz. S. 142 - 156 ; Zsfassung in engl. Sprache
    HBZ-ID HT013663461
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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  5. Book ; Online: Entwicklung von Resistenz- und Virulenzmanagement- Strategien beim Apfelwicklergranulovirus im Ökolo- gischen Obstbau

    Jehle, Johannes / Zebitz, C.P.W. / Buchleither, Sascha

    2023  

    Abstract: Das Ziel des Projekts „Entwicklung von Resistenz- und Virulenzmanagement-Strategien beim Apfelgranulovirus im ökologischen Obstbau“ (Akronym: ÖkoReVir) war es, in enger Zusammenarbeit von Forschung und Praxis eine tragfähige Strategie für ein ... ...

    Abstract Das Ziel des Projekts „Entwicklung von Resistenz- und Virulenzmanagement-Strategien beim Apfelgranulovirus im ökologischen Obstbau“ (Akronym: ÖkoReVir) war es, in enger Zusammenarbeit von Forschung und Praxis eine tragfähige Strategie für ein nachhaltiges Resistenz- und Virulenzmanagement des Apfelwicklergranulovirus (CpGV) zu entwickeln und hieraus Empfehlungen für die Praxis des ökologischen Obstbaus zu erarbeiten. Die Strategie zur Bekämpfung des Apfelwicklers (AW) im ökologischen Obstbau basiert auf einer Kombination verschiedener Bausteine (Pheromon-Verwirrung, Apfelwicklergranuloviren (CpGV), entomopathogenen Nematoden, Bestandshygiene, etc.). Hierbei spielt die Anwendung von CpGV-Präparaten eine zentrale Rolle, da die Präparate für eine effiziente Bekämpfung des Apfelwicklers im ökologischen Anbau unabdingbar sind. Durch das Auftreten von Resistenzen ist die derzeitige Regulierungsstrategie nicht gesichert, langfristig droht der Verlust des wichtigsten Regulierungsbausteins. Zwar konnte in den vergangenen Jahren, durch intensive grundlagen- und praxisnahe Forschungsarbeiten und die Einführung resistenzbrechender CpGV-Isolate, kurzfristig die ökonomische Bedrohung des Öko-Apfelanbaus abgewendet werden, doch ergab sich aus neu beobachteten Resistenztypen in Süd- und Norddeutschland ein erheblicher Forschungs- und Entwicklungsbedarf zur Erarbeitung einer Empfehlung für ein langfristiges Bekämpfungsmanagement. Die Untersuchungen wurden in enger Zusammenarbeit des Instituts für Biologischen Pflanzenschutz des Julius Kühn-Instituts (JKI, Projektkoordination) mit der Universität Hohenheim (HOH), dem Dienstleistungszentrum Ländlicher Raum Rheinpfalz (DLR), dem Öko-Obstbau Norddeutschland (ÖON) und dem Kompetenzzentrum Obstbau Bodensee (KOB) durchgeführt werden.
    Keywords Crop health ; quality ; protection
    Subject code 910
    Language German
    Publishing country dk
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: The distribution and characteristic of two transposable elements in the genome of Cydia pomonella granulovirus and codling moth

    Fan, Jiangbin / Yang, Shili / Wennmann, Jörg T. / Wang, Dun / Jehle, Johannes A.

    Molecular Phylogenetics and Evolution. 2023 May, v. 182 p.107745-

    2023  

    Abstract: Baculoviruses are capable to acquire insect host transposable elements (TEs) in their genomes and are hypothesized as possible vectors of insect transposons between Lepidopteran species. Here, we investigated the host origin of two TEs, namely the Tc1/ ... ...

    Abstract Baculoviruses are capable to acquire insect host transposable elements (TEs) in their genomes and are hypothesized as possible vectors of insect transposons between Lepidopteran species. Here, we investigated the host origin of two TEs, namely the Tc1/mariner-like element TCp3.2 and a 0.7 kbp insertion sequence (IS07), found in the genome of different isolates of Cydia pomonella granulovirus (CpGV), a member of the Betabaculovirus genus. The sequences of both TEs were searched for in the full genome sequence database of codling moth (CM, Cydia pomonella L.). A total of eleven TCp3.2 TE copies and 76 copies of the IS07 fragments were identified in the CM genome. These TEs were distributed over the 22 autosomes and the Z chromosome (chr1) of CM, except chr6, chr12, chr16, chr23, chr27 and the W chromosome (chr29). TCp3.2 copies with two transposase genes in opposite direction, representing a novel feature, were identified on chr10 and chr18. The TCp3.2 transposase was characterized by DD41D motif of classic Tc1/mariner transposons, consisting of DNA-binding domain, catalytic domain and nuclear localization signal (NLS). Transcription analyses of uninfected and CpGV-infected CM larvae suggested a doubling of the TCp3.2 transposase transcription rate in virus infected larvae. Furthermore, IS07 insertion into the CpGV genome apparently added new transcription initiation sites to the viral genome. The global analysis of the distribution of two TEs in the genome of CM addressed the influx of mobile TEs from CM to CpGV, a genetic process that contributes to the population diversity of baculoviruses.
    Keywords Cydia pomonella ; Cydia pomonella granulovirus ; DNA-binding domains ; W chromosome ; Z chromosome ; active sites ; autosomes ; databases ; insects ; nuclear localization signals ; phylogeny ; transcription initiation ; transposases ; transposons ; viral genome ; viruses ; Baculoviridae ; DD41D motif ; Horizontal transfer ; Insertion site ; Transposable elements
    Language English
    Dates of publication 2023-05
    Publishing place Elsevier Inc.
    Document type Article ; Online
    ZDB-ID 33610-5
    ISSN 1095-9513 ; 1055-7903
    ISSN (online) 1095-9513
    ISSN 1055-7903
    DOI 10.1016/j.ympev.2023.107745
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Correction: Fan et al. First Evidence of CpGV Resistance of Codling Moth in the USA.

    Fan, Jiangbin / Jehle, Johannes A / Rucker, Ann / Nielsen, Anne L

    Insects

    2022  Volume 14, Issue 1

    Abstract: In the original publication [ ... ]. ...

    Abstract In the original publication [...].
    Language English
    Publishing date 2022-12-23
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects14010015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: First Evidence of CpGV Resistance of Codling Moth in the USA.

    Fan, Jiangbin / Jehle, Johannes A / Rucker, Ann / Nielsen, Anne L

    Insects

    2022  Volume 13, Issue 6

    Abstract: Codling moth ( ...

    Abstract Codling moth (
    Language English
    Publishing date 2022-06-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects13060533
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Transcriptome of Cydia pomonella granulovirus in susceptible and type I resistant codling moth larvae.

    Wennmann, Jörg T / Pietruska, Diana / Jehle, Johannes A

    The Journal of general virology

    2021  Volume 102, Issue 3

    Abstract: The baculovirus Cydia pomonella granulovirus (CpGV) is a biocontrol agent used worldwide against the codling moth (CM), ...

    Abstract The baculovirus Cydia pomonella granulovirus (CpGV) is a biocontrol agent used worldwide against the codling moth (CM),
    MeSH term(s) Animals ; Europe ; Granulovirus/classification ; Granulovirus/genetics ; Granulovirus/isolation & purification ; Granulovirus/physiology ; Larva/immunology ; Larva/virology ; Moths/growth & development ; Moths/immunology ; Moths/virology ; Plant Diseases/parasitology ; Transcriptome
    Language English
    Publishing date 2021-02-24
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 219316-4
    ISSN 1465-2099 ; 0022-1317
    ISSN (online) 1465-2099
    ISSN 0022-1317
    DOI 10.1099/jgv.0.001566
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Advancing pathogen surveillance by nanopore sequencing and genotype characterization of Acheta domesticus densovirus in mass-reared house crickets.

    Lim, Fang Shiang / González-Cabrera, Joel / Keilwagen, Jens / Kleespies, Regina G / Jehle, Johannes A / Wennmann, Jörg T

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 8525

    Abstract: Rapid and reliable detection of pathogens is crucial to complement the growing industry of mass-reared insects, in order to safeguard the insect colonies from outbreak of diseases, which may cause significant economic loss. Current diagnostic methods are ...

    Abstract Rapid and reliable detection of pathogens is crucial to complement the growing industry of mass-reared insects, in order to safeguard the insect colonies from outbreak of diseases, which may cause significant economic loss. Current diagnostic methods are mainly based on conventional PCR and microscopic examination, requiring prior knowledge of disease symptoms and are limited to identifying known pathogens. Here, we present a rapid nanopore-based metagenomics approach for detecting entomopathogens from the European house cricket (Acheta domesticus). In this study, the Acheta domesticus densovirus (AdDV) was detected from diseased individuals using solely Nanopore sequencing. Virus reads and genome assemblies were obtained within twenty-four hours after sequencing. Subsequently, due to the length of the Nanopore reads, it was possible to reconstruct significantly large parts or even the entire AdDV genome to conduct studies for genotype identification. Variant analysis indicated the presence of three AdDV genotypes within the same house cricket population, with association to the vital status of the diseased crickets. This contrast provided compelling evidence for the existence of non-lethal AdDV genotypes. These findings demonstrated nanopore-based metagenomics sequencing as a powerful addition to the diagnostic tool kit for routine pathogen surveillance and diagnosis in the insect rearing industry.
    MeSH term(s) Humans ; Animals ; Nanopore Sequencing ; Gryllidae ; Densovirus/genetics ; Genotype ; Disease Outbreaks
    Language English
    Publishing date 2024-04-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-58768-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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