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Article ; Online: Biotin as a structural component in the detection of small model antigens in BLA-S-ELISA.

Simonović, Mladen / Ostojić, Sanja / Micić, Darko / Djurdjić, Petar / Mix, Thorsten / Kuzmanović, Čeda / Jelovac, Drago

2024 , Page(s) 1–5

Abstract: An interesting biotin-linked-antigen-Sandwich-ELISA was developed (BLA-S-ELISA), which based on the captured Trinitrophenol-Biotin (TNP-Biotin) molecule between the immobilised monovalent antibody and enzyme-conjugated streptavidin. Monoclonal anti- ... ...

Abstract	An interesting biotin-linked-antigen-Sandwich-ELISA was developed (BLA-S-ELISA), which based on the captured Trinitrophenol-Biotin (TNP-Biotin) molecule between the immobilised monovalent antibody and enzyme-conjugated streptavidin. Monoclonal anti-Trinitrotoluene single chain fragment antibody (anti-TNT-scFv) was cloned and expressed in
Language	English
Publishing date	2024-02-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2185747-7
ISSN	1478-6427 ; 1478-6419
ISSN (online)	1478-6427
ISSN	1478-6419
DOI	10.1080/14786419.2024.2318785
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Article: Streptococcus mitis

Tomic, Uros / Nikolic, Nadja / Carkic, Jelena / Mihailovic, Djordje / Jelovac, Drago / Milasin, Jelena / Pucar, Ana

Pathogens (Basel, Switzerland)

2023 Volume 12, Issue 10

Abstract: Oral microbiome disruptions in periodontitis are related to the chronic inflammatory reactions that could in turn lead to the development of multiple oral diseases. The objective of the study was to assess the frequencies ... ...

Abstract	Oral microbiome disruptions in periodontitis are related to the chronic inflammatory reactions that could in turn lead to the development of multiple oral diseases. The objective of the study was to assess the frequencies of
Language	English
Publishing date	2023-09-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens12101194
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Article ; Online: Pterygoid and tuberosity implants in the atrophic posterior maxilla: A retrospective cohort study.

Konstantinović, Vitomir S / Abd-Ul-Salam, Hani / Jelovac, Drago / Ivanjac, Filip / Miličić, Biljana

The Journal of prosthetic dentistry

2023 Volume 130, Issue 2, Page(s) 219.e1–219.e10

Abstract: Statement of problem: Rehabilitation of the partially or completely edentulous posterior maxilla using dental implants is a clinical challenge because of the presence of the maxillary sinus, as well as the low quality and quantity of bone in that region. ...

Abstract	Statement of problem: Rehabilitation of the partially or completely edentulous posterior maxilla using dental implants is a clinical challenge because of the presence of the maxillary sinus, as well as the low quality and quantity of bone in that region. In addition to bone augmentation procedures, posterior maxillary rehabilitation using implants includes their anchoring in bones such as the zygoma, pterygoid, and maxillary tuberosity, as well as in short implants. However, the performance of pterygoid and tuberosity implants in the atrophic posterior maxilla is unclear. Purpose: The purpose of this retrospective cohort study was to evaluate the survival of tuberosity and pterygoid implants in patients with posterior maxillary atrophy. Material and methods: A nonprobability convenient sample of patients who had received fixed prostheses on implants placed in the maxillary tuberosity or pterygoid regions was analyzed retrospectively. Demographic variables included sex (male, female) and age. Implant-related variables included surface characteristics, site of placement, implant design, length, diameter, and anteroposterior insertion angle. Prosthetic-related variables included the type of reconstruction for rehabilitation and loading protocols. Implant survival, complications, crestal bone loss, and follow-up intervals were also documented. Collected data were analyzed at both patient and implant levels. The demographics and implant characteristics of patients receiving pterygoid or tuberosity implants were analyzed with a statistical software program (α=.05). Survival analysis was estimated by using the nonparametric Kaplan-Meier curve. Results: A total of 119 patients had 183 pterygoid or tuberosity implants inserted. Most implants in the pterygoid region (71.5%) were Ø4.1 mm (87.4%) and 15 mm in length (60.1%). The most common prostheses were complete maxillary reconstructions (49.2%) with late loading (74.3%). The average implant anteroposterior insertion angle was 60.8 degrees. The cumulative survival rate was 97.3% (n=178) during the mean follow-up period of 57 months (range 1 to 168 months). Among all implants placed, 2.7% failed (n=5) within 2 months of their placement. The statistically significant differences noted between tuberosity and pterygoid implants were related to design, surface characteristics, and loading. The average crestal bone loss was 1.5 mm. Conclusions: The survival of the implants placed in the maxillary tuberosity and pterygoid regions was high in patients with posterior maxillary atrophy.
MeSH term(s)	Humans ; Female ; Male ; Maxilla/surgery ; Retrospective Studies ; Dental Implants ; Head ; Atrophy ; Bone Diseases, Metabolic
Chemical Substances	Dental Implants
Language	English
Publishing date	2023-07-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	218157-5
ISSN	1097-6841 ; 0022-3913
ISSN (online)	1097-6841
ISSN	0022-3913
DOI	10.1016/j.prosdent.2023.06.007
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Article ; Online: Osteogenic and Adipogenic Differentiation Potential of Oral Cancer Stem Cells May Offer New Treatment Modalities.

Jaksic Karisik, Milica / Lazarevic, Milos / Mitic, Dijana / Nikolic, Nadja / Milosevic Markovic, Maja / Jelovac, Drago / Milasin, Jelena

International journal of molecular sciences

2023 Volume 24, Issue 5

Abstract: 1) Treatment failure of oral squamous cell carcinoma (OSCC) is generally due to the development of therapeutic resistance caused by the existence of cancer stem cells (CSCs), a small cell subpopulation with marked self-renewal and differentiation ... ...

Abstract	(1) Treatment failure of oral squamous cell carcinoma (OSCC) is generally due to the development of therapeutic resistance caused by the existence of cancer stem cells (CSCs), a small cell subpopulation with marked self-renewal and differentiation capacity. Micro RNAs, notably miRNA-21, appear to play an important role in OSCC carcinogenesis. Our objectives were to explore the multipotency of oral CSCs by estimating their differentiation capacity and assessing the effects of differentiation on stemness, apoptosis, and several miRNAs' expression. (2) A commercially available OSCC cell line (SCC25) and five primary OSCC cultures generated from tumor tissues obtained from five OSCC patients were used in the experiments. Cells harboring CD44, a CSC marker, were magnetically separated from the heterogeneous tumor cell populations. The CD44
MeSH term(s)	Humans ; Carcinoma, Squamous Cell/pathology ; MicroRNAs/metabolism ; Mouth Neoplasms/pathology ; Neoplastic Stem Cells/cytology ; Neoplastic Stem Cells/metabolism ; Adipogenesis ; Osteogenesis
Chemical Substances	MicroRNAs ; MIRN491 microRNA, human
Language	English
Publishing date	2023-02-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24054704
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Article ; Online: Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer.

Eljabo, N / Nikolic, N / Carkic, J / Jelovac, D / Lazarevic, M / Tanic, N / Milasin, J

International journal of oral and maxillofacial surgery

2018 Volume 47, Issue 8, Page(s) 976–982

Abstract: Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The ... ...

Abstract	Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P<0.001 and P=0.027, respectively). HER2 amplification in margin tissue (P<0.001) and swabs (P=0.013), as well as the existence of three co-alterations in margins (P=0.001) and macroscopically unaffected oral mucosa (P<0.001) were correlated with shorter disease-specific survival.
MeSH term(s)	Aged ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/pathology ; Carcinoma, Squamous Cell/virology ; DNA Primers ; Epigenomics ; Humans ; Mouth Mucosa/pathology ; Mouth Neoplasms/genetics ; Mouth Neoplasms/pathology ; Mouth Neoplasms/virology ; Neoplasm Recurrence, Local/genetics ; Neoplasm Recurrence, Local/pathology ; Neoplasm Staging ; Prognosis ; Promoter Regions, Genetic ; Real-Time Polymerase Chain Reaction ; Survival Rate
Chemical Substances	DNA Primers
Language	English
Publishing date	2018-02-12
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	353721-3
ISSN	1399-0020 ; 0901-5027
ISSN (online)	1399-0020
ISSN	0901-5027
DOI	10.1016/j.ijom.2018.01.020
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Zs.A 931: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 401: Show issues

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Article ; Online: Parental Satisfaction from Telemedicine in the Follow-up of Children Operated for Craniosynostosis during COVID-19 Pandemic.

Kilipiris, Evangelos G / Horn, Frantisek / Kolnikova, Miroslava / Ochoa, Jose Vallejo / Matuskova, Olga / Jelovac, Drago / Stebel, Adam

The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association

2022 Volume 60, Issue 5, Page(s) 562–568

Abstract: Objective: The current study aimed to evaluate the satisfaction level of parents from telemedicine use in the long-term follow-up of children operated for craniosynostosis during the COVID-19 pandemic.: Design: A cross-sectional 9-question survey ... ...

Abstract	Objective: The current study aimed to evaluate the satisfaction level of parents from telemedicine use in the long-term follow-up of children operated for craniosynostosis during the COVID-19 pandemic. Design: A cross-sectional 9-question survey analysis using Qualtrics survey software. Setting: An institutional study carried out at a national, tertiary level academic center in the Slovak Republic. Patients: All patients operated for non-syndromic and syndromic craniosynostosis in our department, who participated in the virtual postoperative follow-up consultations from March 23, 2020, through July 2, 2021, were included in our series. They were enrolled 498 patients, with the parents of 256 children being responding to the survey. Interventions: The survey remained open until July 16, 2021, two weeks after the last virtual consultations. It was delivered to the parents by e-mail to generate closed 5-point Likert scale responses. Results: Overall, 72.3% of parents were satisfied with the telemedicine program, and 67.2% found it convenient. Collectively, 89.1% reported that the received instructions were helpful to them. However, only 18.7% of them answered that they would prefer telemedicine consultations in the future. Conclusion: The results of this study showed a high satisfaction from the parents during the virtual follow-up of children operated for craniosynostosis. However, despite their positive experience with telemedicine, the vast majority of parents stated that they would prefer in-person visits in the future.
MeSH term(s)	Humans ; Child ; COVID-19 ; Cross-Sectional Studies ; Follow-Up Studies ; Pandemics ; Telemedicine ; Craniosynostoses/surgery ; Parents ; Patient Satisfaction
Language	English
Publishing date	2022-01-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	1069409-2
ISSN	1545-1569 ; 0009-8701 ; 1055-6656
ISSN (online)	1545-1569
ISSN	0009-8701 ; 1055-6656
DOI	10.1177/10556656221074214
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Zs.A 451: Show issues

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Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: Immediate placement of extra-short implants in refined scapula tip microvascular free flaps: In house virtual planning and surgical technique - Proof of concept.

Jelovac, Drago / Micic, Milutin / Hajdarevic, Sanela / Kuzmanovic, Cedomir / Cukic, Branislav / Stefanovic, Branislav / Zelic, Ksenija / Bonfante, E A / Ewers, Rolf / Petrovic, Milan

Heliyon

2023 Volume 9, Issue 7, Page(s) e18021

Abstract: Scapula tip flaps have been introduced in the literature as an ideal surgical treatment option for large defects in the horizontal plane of the maxilla. This article aims to present a unique step by step protocol for a near total maxillectomy with a ... ...

Abstract	Scapula tip flaps have been introduced in the literature as an ideal surgical treatment option for large defects in the horizontal plane of the maxilla. This article aims to present a unique step by step protocol for a near total maxillectomy with a pterygoid bone resection and consecutive microvascular reconstruction with a harvested scapula tip flap. The protocol includes immediate placement of extra-short implants in donor bone with the aid of Virtual Surgical Planning (VSP), and an in-house 3D printing of medical 3D models and surgical guides. So far, there has been no presented surgical technique combining immediate implant placement in the scapula region with simultaneous microvascular repair. This technique allows: tumour resection; flap harvesting; extra-short implant placements and reconstruction to be performed in one simultaneous procedure. The technique is presented with illustrations, VSP (presented on videos), radiographs, and surgical findings. We discovered that this refinement of the scapula tip surgery has enabled reconstructive procedures to be performed at the same time as implant placements, providing expedited functional and aesthetic outcomes in selected cases. Moreover, modification of the surgical technique could enhance the competence of the oropharyngeal edge. In conclusion, this new surgical protocol utilizing VSP, 3D models and simultaneous extra-short implant placement provides indispensable advantages for such a complicated surgical procedures, while significantly shortening the duration of surgery.
Language	English
Publishing date	2023-07-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e18021
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Article ; Online: A randomized phase II evaluation of weekly gemcitabine plus pazopanib versus weekly gemcitabine alone in the treatment of persistent or recurrent epithelial ovarian, fallopian tube or primary peritoneal carcinoma.

Duska, L R / Petroni, G R / Varhegyi, N / Brown, J / Jelovac, D / Moore, K N / McGuire, W P / Darus, C / Barroilhet, L M / Secord, A A

Gynecologic oncology

2020 Volume 157, Issue 3, Page(s) 585–592

Abstract: Objective: Angiogenesis inhibition is a valuable strategy for ovarian cancer (EOC). Pazopanib (paz) is a potent small molecular inhibitor of VEGF-1, -2, -3, PDGFR, c-kit, and has activity as a single agent in ovarian cancer. We designed a trial to ... ...

Abstract	Objective: Angiogenesis inhibition is a valuable strategy for ovarian cancer (EOC). Pazopanib (paz) is a potent small molecular inhibitor of VEGF-1, -2, -3, PDGFR, c-kit, and has activity as a single agent in ovarian cancer. We designed a trial to assess the benefit of adding paz to gemcitabine (gem) in patients with recurrent EOC. Methods: An open-label, randomized, multi-site, phase 2 trial was conducted (NCT01610206) including patients with platinum resistant or sensitive disease, ≤ 3 prior lines of chemotherapy, and measurable/evaluable disease. Patients were randomly assigned to weekly gem 1000 mg/m Results: 148 patients were enrolled 2012-2017. Median age was 63 years (30-82); 60% were platinum resistant; median surveillance was 13 months (0.4-54 months). Median PFS was 5.3 (95% CI, 4.2-5.8) vs 2.9 months (95% CI, 2.1-4.1) in the gem arm. The PFS effect was most pronounced in the platinum resistant group (5.32 vs 2.33 months Tarone-Ware p < 0.001). There was no difference in OS. Overall RR (PR 20% vs 11%, Chi-squre p = 0.02) and DCR (80% vs 60%, Chi-square p < 0.001) were higher in the combination. High grade AEs in the combination arm included ≥ Grade 3: hypertension (15%), neutropenia (35%), and thrombocytopenia (12%). Conclusions: The addition of paz to gem enhanced anti-tumor activity; those with platinum-resistant disease derived the most benefit from combination therapy, even in the setting of receiving prior bevacizumab.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Carcinoma, Ovarian Epithelial/drug therapy ; Carcinoma, Ovarian Epithelial/pathology ; Deoxycytidine/analogs & derivatives ; Deoxycytidine/pharmacology ; Deoxycytidine/therapeutic use ; Fallopian Tube Neoplasms/drug therapy ; Fallopian Tube Neoplasms/pathology ; Female ; Humans ; Middle Aged ; Peritoneal Neoplasms/drug therapy ; Peritoneal Neoplasms/pathology ; Pyrimidines/pharmacology ; Pyrimidines/therapeutic use ; Sulfonamides/pharmacology ; Sulfonamides/therapeutic use
Chemical Substances	Pyrimidines ; Sulfonamides ; Deoxycytidine (0W860991D6) ; pazopanib (7RN5DR86CK) ; gemcitabine (B76N6SBZ8R)
Language	English
Publishing date	2020-04-01
Publishing country	United States
Document type	Clinical Trial, Phase II ; Journal Article ; Multicenter Study ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	801461-9
ISSN	1095-6859 ; 0090-8258
ISSN (online)	1095-6859
ISSN	0090-8258
DOI	10.1016/j.ygyno.2019.10.014
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Zs.A 885: Show issues

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Article: HER2-directed therapy for metastatic breast cancer.

Jelovac, Danijela / Emens, Leisha A

Oncology (Williston Park, N.Y.)

2013 Volume 27, Issue 3, Page(s) 166–175

Abstract: Human epidermal growth factor receptor 2 (HER2) overexpression drives the biology of 20% of breast cancers, and predicts a poor prognosis for patients. HER2-targeted therapies significantly improve outcomes for HER2-positive patients with both early and ... ...

Abstract	Human epidermal growth factor receptor 2 (HER2) overexpression drives the biology of 20% of breast cancers, and predicts a poor prognosis for patients. HER2-targeted therapies significantly improve outcomes for HER2-positive patients with both early and metastatic breast cancer. Currently three HER2-targeted agents, trastuzumab (Herceptin), lapatinib (Tykerb), and pertuzumab (Perjeta), are available for the treatment of HER2-positive metastatic breast cancer (MBC). Numerous studies have attempted to optimize their use by combining them with each other, or with endocrine and cytotoxic therapies. Most recently, the FDA approved the combination of trastuzumab, pertuzumab, and docetaxel as first-line treatment for MBC, and in late February 2013 approved a fourth HER2-targeted agent, trastuzumab emtansine (T-DM1, Kadcyla), for accelerated approval. These advances create a number of clinical dilemmas, including identification of the optimal sequence of HER2-targeted agents and the best drug combinations to use, as well as the recognition of primary and acquired drug resistance. In this article, we review clinical data informing the effective management of HER2-positive MBC.
MeSH term(s)	Antineoplastic Agents/therapeutic use ; Breast Neoplasms/drug therapy ; Breast Neoplasms/secondary ; Female ; Humans ; Molecular Targeted Therapy ; Receptor, ErbB-2/antagonists & inhibitors
Chemical Substances	Antineoplastic Agents ; Receptor, ErbB-2 (EC 2.7.10.1)
Language	English
Publishing date	2013-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1067950-9
ISSN	0890-9091
ISSN	0890-9091
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Zs.A 3168: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 372: Show issues

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Article ; Online: Prevalence of chemopreventive agent use among hospitalised women at high risk for breast cancer: a cross-sectional study.

Khaliq, Waseem / Jelovac, Danijela / Wright, Scott M

BMJ open

2016 Volume 6, Issue 11, Page(s) e012550

Abstract: Objective: To characterise the current usage of chemoprevention agents among hospitalised women who are at higher risk for breast cancer.: Study design: A cross-sectional study.: Setting: Academic hospital at Baltimore.: Participants: A bedside ...

Abstract	Objective: To characterise the current usage of chemoprevention agents among hospitalised women who are at higher risk for breast cancer. Study design: A cross-sectional study. Setting: Academic hospital at Baltimore. Participants: A bedside survey of 250 women aged 50-75 years was conducted who were cancer-free at the time of study enrolment and hospitalised to a general medicine service. Reproductive history, family history for breast cancer, chemopreventive agents use and medical comorbidities data was collected for all patients. χ Primary outcome measures: Prevalence of women at high risk for developing breast cancer (5-year Gail risk score ≥1.7) and their chemopreventive agent use. Results: Mean age for the study population was 61.5 years (SD 7.5), and mean 5-year Gail risk score was 1.67 (SD 0.88). A third of study population was at high risk for breast cancer. None of the high-risk women (0%) were taking chemoprevention for breast cancer risk reduction, and 23% were at very high risk with 5-year Gail score ≥3%. These women were not recognised as being high risk by their hospital providers and none were referred to the high-risk breast cancer clinics following discharge. Conclusions: Many hospitalised women are at high risk for breast cancer and we could not identify even a single woman who was using chemoprevention for risk reduction. Current chemoprevention guidelines may be falling short in their dissemination and implementation. Since women at high risk for breast cancer may only interface with the healthcare system at select points, all healthcare providers must be willing and able to do risk assessment. For those identified to be at high risk, providers must then either engage in chemopreventive counselling or refer patients to providers who are more comfortable working with patients on this critical decision.
MeSH term(s)	Academic Medical Centers ; Age Distribution ; Aged ; Anticarcinogenic Agents/therapeutic use ; Breast Neoplasms/drug therapy ; Breast Neoplasms/epidemiology ; Chemoprevention/statistics & numerical data ; Comorbidity ; Cross-Sectional Studies ; Female ; Hospitalization ; Humans ; Maryland ; Middle Aged ; Risk Assessment ; Risk Reduction Behavior ; Severity of Illness Index ; Surveys and Questionnaires
Chemical Substances	Anticarcinogenic Agents
Language	English
Publishing date	2016-11-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2747269-3
ISSN	2044-6055 ; 2044-6055 ; 2053-3624
ISSN (online)	2044-6055
ISSN	2044-6055 ; 2053-3624
DOI	10.1136/bmjopen-2016-012550
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