LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Jenny Zhaoying Xiang"
  2. AU=Ferraro Elisabetta
  3. AU="Jonathan Downar"
  4. AU=Rahmanzade Ramin AU=Rahmanzade Ramin
  5. AU="Edwards, Lisa-Jayne"
  6. AU="Tominaga, Shintaro"
  7. AU="Chan, Brian"
  8. AU="Julieta Carilla"

Suchergebnis

Treffer 1 - 8 von insgesamt 8

Suchoptionen

  1. Artikel ; Online: Vaccinia E5 is a major inhibitor of the DNA sensor cGAS

    Ning Yang / Yi Wang / Peihong Dai / Tuo Li / Christian Zierhut / Adrian Tan / Tuo Zhang / Jenny Zhaoying Xiang / Alban Ordureau / Hironori Funabiki / Zhijian Chen / Liang Deng

    Nature Communications, Vol 14, Iss 1, Pp 1-

    2023  Band 16

    Abstract: Abstract The DNA sensor cyclic GMP-AMP synthase (cGAS) is critical in host antiviral immunity. Vaccinia virus (VACV) is a large cytoplasmic DNA virus that belongs to the poxvirus family. How vaccinia virus antagonizes the cGAS-mediated cytosolic DNA- ... ...

    Abstract Abstract The DNA sensor cyclic GMP-AMP synthase (cGAS) is critical in host antiviral immunity. Vaccinia virus (VACV) is a large cytoplasmic DNA virus that belongs to the poxvirus family. How vaccinia virus antagonizes the cGAS-mediated cytosolic DNA-sensing pathway is not well understood. In this study, we screened 80 vaccinia genes to identify potential viral inhibitors of the cGAS/Stimulator of interferon gene (STING) pathway. We discovered that vaccinia E5 is a virulence factor and a major inhibitor of cGAS. E5 is responsible for abolishing cGAMP production during vaccinia virus (Western Reserve strain) infection of dendritic cells. E5 localizes to the cytoplasm and nucleus of infected cells. Cytosolic E5 triggers ubiquitination of cGAS and proteasome-dependent degradation via interacting with cGAS. Deleting the E5R gene from the Modified vaccinia virus Ankara (MVA) genome strongly induces type I IFN production by dendritic cells (DCs) and promotes DC maturation, and thereby improves antigen-specific T cell responses.
    Schlagwörter Science ; Q
    Thema/Rubrik (Code) 570
    Sprache Englisch
    Erscheinungsdatum 2023-05-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  2. Artikel ; Online: Derivation and characterization of a UCP1 reporter human ES cell line

    Suranjit Mukherjee / Tuo Zhang / Lauretta A. Lacko / Lei Tan / Jenny Zhaoying Xiang / Jason M. Butler / Shuibing Chen

    Stem Cell Research, Vol 30, Iss , Pp 12-

    2018  Band 21

    Abstract: Interest in human brown fat as a novel therapeutic target to tackle the growing obesity and diabetes epidemic has increased dramatically in recent years. While much insight into brown fat biology has been gained from murine cell lines and models, few ... ...

    Abstract Interest in human brown fat as a novel therapeutic target to tackle the growing obesity and diabetes epidemic has increased dramatically in recent years. While much insight into brown fat biology has been gained from murine cell lines and models, few resources are available to study human brown fat in vitro, which makes the need for new ways to derive and study human brown adipocytes imperative. Human ES cell based reporter systems present an excellent tool to identify, mark, and purify cell populations of choice. In this study, we detail the derivation and characterization of a novel human ES UCP1 reporter cell line that marks UCP1 positive adipocytes in vitro. We targeted a mCherry reporter to the UCP1 stop codon via CRISPR-Cas9 based gene targeting. The brown adipocytes derived from reporter cells express UCP1, display high mitochondrial content, multi-locular lipid morphology, and exhibit functional properties such as lipolysis. The mCherry positive cells purified after cell sorting show elevated expression of brown fat marker genes and a high similarity to isolated human brown fat via RNA-seq analysis. Finally, we demonstrate the utility of this reporter to real time monitor UCP1 expression upon stimulation. This reporter cell line thus presents new opportunities to study human brown fat biology by enabling future work to understand early human brown fat development, perform disease modeling, and facilitate drug screening.
    Schlagwörter Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 616
    Sprache Englisch
    Erscheinungsdatum 2018-07-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  3. Artikel ; Online: A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells

    Zaniar Ghazizadeh / Jiajun Zhu / Faranak Fattahi / Alice Tang / Xiaolu Sun / Sadaf Amin / Su-Yi Tsai / Mona Khalaj / Ting Zhou / Ryan M. Samuel / Tuo Zhang / Francis A. Ortega / Miriam Gordillo / Dorota Moroziewicz / Daniel Paull / Scott A. Noggle / Jenny Zhaoying Xiang / Lorenz Studer / David J. Christini /
    Geoffrey S. Pitt / Todd Evans / Shuibing Chen

    iScience, Vol 25, Iss 4, Pp 104153- (2022)

    2022  

    Abstract: Summary: The sinoatrial node (SAN) is the primary pacemaker of the heart. The human SAN is poorly understood due to limited primary tissue access and limitations in robust in vitro derivation methods. We developed a dual SHOX2:GFP; MYH6:mCherry knockin ... ...

    Abstract Summary: The sinoatrial node (SAN) is the primary pacemaker of the heart. The human SAN is poorly understood due to limited primary tissue access and limitations in robust in vitro derivation methods. We developed a dual SHOX2:GFP; MYH6:mCherry knockin human embryonic stem cell (hESC) reporter line, which allows the identification and purification of SAN-like cells. Using this line, we performed several rounds of chemical screens and developed an efficient strategy to generate and purify hESC-derived SAN-like cells (hESC-SAN). The derived hESC-SAN cells display molecular and electrophysiological characteristics of bona fide nodal cells, which allowed exploration of their transcriptional profile at single-cell level. In sum, our dual reporter system facilitated an effective strategy for deriving human SAN-like cells, which can potentially be used for future disease modeling and drug discovery.
    Schlagwörter Biological sciences ; Stem cells research ; Methodology in biological sciences ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2022-04-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  4. Artikel ; Online: Using hESCs to Probe the Interaction of the Diabetes-Associated Genes CDKAL1 and MT1E

    Min Guo / Tuo Zhang / Xue Dong / Jenny Zhaoying Xiang / Minxiang Lei / Todd Evans / Johannes Graumann / Shuibing Chen

    Cell Reports, Vol 19, Iss 8, Pp 1512-

    2017  Band 1521

    Abstract: Genome-wide association studies (GWASs) have identified many disease-associated variant alleles, but understanding whether and how different genes/loci interact requires a platform for probing how the variant alleles act mechanistically. Isogenic mutant ... ...

    Abstract Genome-wide association studies (GWASs) have identified many disease-associated variant alleles, but understanding whether and how different genes/loci interact requires a platform for probing how the variant alleles act mechanistically. Isogenic mutant human embryonic stem cells (hESCs) provide an unlimited resource to derive and study human disease-relevant cells. Here, we focused on CDKAL1, linked by GWASs to diabetes. Through transcript profiling, we find that expression of the metallothionein (MT) gene family, also linked by GWASs to diabetes, is significantly downregulated in CDKAL1−/− cells that have been differentiated to insulin-expressing pancreatic beta-like cells. Forced MT1E expression rescues both hypersensitivity of CDKAL1 mutant cells to glycolipotoxicity and pancreatic beta-cell dysfunction in vitro and in vivo. MT1E functions at least in part through relief of ER stress. This study establishes an isogenic hESC-based platform to study the interaction of GWAS-identified diabetes gene variants and illuminate the molecular network impacting disease progression.
    Schlagwörter metallothionein ; CDKAL1 ; human pancreatic beta-like cells ; directed differentiation ; humanized mouse ; chemical chaperones ; endoplasmic reticulum stress ; ER ; Biology (General) ; QH301-705.5
    Sprache Englisch
    Erscheinungsdatum 2017-05-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  5. Artikel ; Online: Discovery of a drug candidate for GLIS3-associated diabetes

    Sadaf Amin / Brandoch Cook / Ting Zhou / Zaniar Ghazizadeh / Raphael Lis / Tuo Zhang / Mona Khalaj / Miguel Crespo / Manuradhi Perera / Jenny Zhaoying Xiang / Zengrong Zhu / Mark Tomishima / Chengyang Liu / Ali Naji / Todd Evans / Danwei Huangfu / Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 12

    Abstract: GLIS3 mutations are associated with type 1, type 2, and neonatal diabetes. Here, the authors generate mono-hormonal glucose-responding pancreatic β-like cells in vitro and through a screen identify a drug that rescues pancreatic β-like cell death in ... ...

    Abstract GLIS3 mutations are associated with type 1, type 2, and neonatal diabetes. Here, the authors generate mono-hormonal glucose-responding pancreatic β-like cells in vitro and through a screen identify a drug that rescues pancreatic β-like cell death in GLIS3 mutants by inhibiting the abnormally activated TGFβ pathway.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-07-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  6. Artikel ; Online: Discovery of a drug candidate for GLIS3-associated diabetes

    Sadaf Amin / Brandoch Cook / Ting Zhou / Zaniar Ghazizadeh / Raphael Lis / Tuo Zhang / Mona Khalaj / Miguel Crespo / Manuradhi Perera / Jenny Zhaoying Xiang / Zengrong Zhu / Mark Tomishima / Chengyang Liu / Ali Naji / Todd Evans / Danwei Huangfu / Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 12

    Abstract: GLIS3 mutations are associated with type 1, type 2, and neonatal diabetes. Here, the authors generate mono-hormonal glucose-responding pancreatic β-like cells in vitro and through a screen identify a drug that rescues pancreatic β-like cell death in ... ...

    Abstract GLIS3 mutations are associated with type 1, type 2, and neonatal diabetes. Here, the authors generate mono-hormonal glucose-responding pancreatic β-like cells in vitro and through a screen identify a drug that rescues pancreatic β-like cell death in GLIS3 mutants by inhibiting the abnormally activated TGFβ pathway.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-07-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  7. Artikel ; Online: A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival

    Ting Zhou / Tae Wan Kim / Chi Nok Chong / Lei Tan / Sadaf Amin / Zohreh Sadat Badieyan / Suranjit Mukherjee / Zaniar Ghazizadeh / Hui Zeng / Min Guo / Miguel Crespo / Tuo Zhang / Reyn Kenyon / Christopher L. Robinson / Effie Apostolou / Hui Wang / Jenny Zhaoying Xiang / Todd Evans / Lorenz Studer /
    Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 13

    Abstract: Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that ... ...

    Abstract Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that loss of the gene GSTT1 confers hypersensitivity.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-11-01T00:00:00Z
    Verlag Nature Publishing Group
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  8. Artikel ; Online: A hPSC-based platform to discover gene-environment interactions that impact human β-cell and dopamine neuron survival

    Ting Zhou / Tae Wan Kim / Chi Nok Chong / Lei Tan / Sadaf Amin / Zohreh Sadat Badieyan / Suranjit Mukherjee / Zaniar Ghazizadeh / Hui Zeng / Min Guo / Miguel Crespo / Tuo Zhang / Reyn Kenyon / Christopher L. Robinson / Effie Apostolou / Hui Wang / Jenny Zhaoying Xiang / Todd Evans / Lorenz Studer /
    Shuibing Chen

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Band 13

    Abstract: Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that ... ...

    Abstract Diseases such as diabetes and Parkinson's manifest based on interactions between genes and environment. Here, the authors find among a panel of cell types that propargite, a common pesticide, induces pancreatic β-cell and dopamine neuron death and that loss of the gene GSTT1 confers hypersensitivity.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2018-11-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Zum Seitenanfang