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  1. Article ; Online: Blood metabolomics and impacted cellular mechanisms during transition into lactation in dairy cows that develop metritis.

    Casaro, S / Prim, J G / Gonzalez, T D / Figueiredo, C C / Bisinotto, R S / Chebel, R C / Santos, J E P / Nelson, C D / Jeon, S J / Bicalho, R C / Driver, J P / Galvão, K N

    Journal of dairy science

    2023  Volume 106, Issue 11, Page(s) 8098–8109

    Abstract: The objective of this study was to identify metabolites associated with metritis and use them for identification of cellular mechanisms affected during transition into lactation. Holstein cows (n = 104) had blood collected in the prepartum period (d -14 ± ...

    Abstract The objective of this study was to identify metabolites associated with metritis and use them for identification of cellular mechanisms affected during transition into lactation. Holstein cows (n = 104) had blood collected in the prepartum period (d -14 ± 6 relative to calving), at calving (d 0), and at the day of metritis diagnosis (d 7 ± 2 after calving). Cows with reddish or brownish, watery, and fetid discharge were diagnosed with metritis (n = 52). Cows with metritis were paired with herdmates without metritis (n = 52) based on days in milk. The metabolome of plasma samples was evaluated using untargeted gas chromatography time-of-flight mass spectrometry. Univariate analyses included t-tests and fold change analyses. Metabolites with false discovery rate adjusted P ≤ 0.10 on t-tests were used for partial least squares discriminant analysis coupled with permutational analysis using 2,000 permutations. Metabolites with false discovery rate adjusted P ≤ 0.10 on t-tests were also used for enriched pathway analyses and identification of cellular processes. Cows that developed metritis had affected cellular processes associated with lower amino acid metabolism in the prepartum period, greater lipolysis, cell death, and oxidative stress at calving and at metritis diagnosis, and greater leukocyte activation at calving, but lower immune cell activation at metritis diagnosis. In summary, cows that developed metritis had plasma metabolomic changes associated with greater lipolysis, oxidative stress, and a dysregulated immune response which may predispose cows to metritis development.
    Language English
    Publishing date 2023-08-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 242499-x
    ISSN 1525-3198 ; 0022-0302
    ISSN (online) 1525-3198
    ISSN 0022-0302
    DOI 10.3168/jds.2023-23433
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  2. Article ; Online: Hepatitis A virus strains identified in jogaejeot associated with outbreaks in Seoul, South Korea.

    Jeong, H W / Kim, M K / Yi, H J / Kim, D M / Jeon, S J / Lee, H K / Oh, Y H / Hwang, Y O

    Letters in applied microbiology

    2021  Volume 73, Issue 1, Page(s) 107–112

    Abstract: Jogaejeot, seasoned Venerupis philippinarum, is a traditional Korean fermented food, and hepatitis A virus (HAV) can be transmitted through contaminated food, especially bivalve shellfish, causing acute gastroenteritis worldwide. Here, we carried out a ... ...

    Abstract Jogaejeot, seasoned Venerupis philippinarum, is a traditional Korean fermented food, and hepatitis A virus (HAV) can be transmitted through contaminated food, especially bivalve shellfish, causing acute gastroenteritis worldwide. Here, we carried out a phylogenetic analysis to identify and characterize HAV strains in jogaejeot samples associated with hepatitis A (HA) outbreaks in Seoul, South Korea, in 2019. The HAV strains were identified using blast and molecular analysis of the amplified HAV VP1-P2B genome region. The HAV strains identified in the five jogaejeot samples shared at least 99% sequence identity, were all classified as genotype IA and were most closely related to strains that are widespread in East Asia. These results support a link between the consumption of jogaejeot and the HA outbreaks observed in 2019 in Seoul. In addition, they indicate a need for more stringent enforcement of food safety regulations for the shellfish industry, especially against HAV, and the value of widespread vaccination.
    MeSH term(s) Animals ; Bivalvia/virology ; Disease Outbreaks ; Fermented Foods and Beverages/virology ; Food Safety ; Genotype ; Hepatitis A/epidemiology ; Hepatitis A/prevention & control ; Hepatitis A/virology ; Hepatitis A virus/classification ; Hepatitis A virus/genetics ; Humans ; Phylogeny ; RNA, Viral/genetics ; Seoul/epidemiology ; Shellfish/virology ; Vaccination
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-05-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 632584-1
    ISSN 1472-765X ; 0266-8254
    ISSN (online) 1472-765X
    ISSN 0266-8254
    DOI 10.1111/lam.13482
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  3. Article ; Online: Effect of pegbovigrastim administration on the microbiome found in the vagina of cows postpartum.

    Galvão, K N / Higgins, C H / Zinicola, M / Jeon, S J / Korzec, H / Bicalho, R C

    Journal of dairy science

    2019  Volume 102, Issue 4, Page(s) 3439–3451

    Abstract: The objective of this study was to evaluate the effect of pegbovigrastim (PEG) treatment of peripartum Holstein cows on the microbiome found in the vagina postpartum using sequencing of the 16S rRNA gene. A subset of cows was randomly sampled from a ... ...

    Abstract The objective of this study was to evaluate the effect of pegbovigrastim (PEG) treatment of peripartum Holstein cows on the microbiome found in the vagina postpartum using sequencing of the 16S rRNA gene. A subset of cows was randomly sampled from a larger study where cows had been randomly assigned to 1 of 2 treatments: pegbovigrastim (PEG) or untreated control (CTR). The PEG-treated cows received a subcutaneous injection containing 15 mg of pegbovigrastim 7 d before expected calving and a second injection within 24 h of calving. Vaginal samples from 97 PEG-treated and 98 CTR cows were collected at calving, 7 ± 3, and 35 ± 3 d in milk (0, 7, and 35 DIM). Metritis was diagnosed at 7 ± 3 DIM and purulent vaginal discharge (PVD) at 35 ± 3 DIM. The PEG treatment did not alter the vaginal microbiome. Principal coordinate analysis (PCoA) showed that metritic cows had a dissimilar vaginal microbiome compared with cows that did not develop metritis, particularly at 7 but also at 35 DIM. This difference was characterized by higher relative abundance of Porphyromonas and Bacteroides and a lower relative abundance of Ureaplasma, Ruminococcaceae, and Clostridiales at 7 DIM, and a higher relative abundance of Ureaplasma and a lower relative abundance of Pasteurellaceae at 35 DIM. Based on PCoA, we observed that cows that developed PVD had a dissimilar vaginal microbiome compared with cows that did not develop PVD, particularly at 35 DIM but also at 7 DIM. This difference was characterized by a higher relative abundance of Bacteroides at 7 DIM and higher relative abundance of Fusobacterium and Bacteroides at 35 DIM. Cows that developed metritis and PVD also had higher relative abundance of Fusobacterium and Bacteroides at 0 DIM. Furthermore, the Chao1 and Shannon indices were decreased in metritic cows at 7 DIM and in PVD cows at 7 and 35 DIM. In summary, PEG treatment had no effect on the vaginal microbiome, and uterine disease was associated with major changes in the microbiome found in the vagina postpartum.
    MeSH term(s) Animals ; Bacteroides ; Cattle ; Cattle Diseases/microbiology ; Cattle Diseases/prevention & control ; Colony-Stimulating Factors/pharmacology ; Endometritis/prevention & control ; Endometritis/veterinary ; Female ; Granulocyte Colony-Stimulating Factor/administration & dosage ; Granulocyte Colony-Stimulating Factor/pharmacology ; Microbiota/drug effects ; Postpartum Period ; RNA, Ribosomal, 16S ; Random Allocation ; Recombinant Proteins/administration & dosage ; Recombinant Proteins/pharmacology ; Uterine Diseases ; Vagina/microbiology
    Chemical Substances Colony-Stimulating Factors ; RNA, Ribosomal, 16S ; Recombinant Proteins ; Granulocyte Colony-Stimulating Factor (143011-72-7) ; pegbovigrastim (87M3B1263R)
    Language English
    Publishing date 2019-02-22
    Publishing country United States
    Document type Clinical Trial, Veterinary ; Journal Article
    ZDB-ID 242499-x
    ISSN 1525-3198 ; 0022-0302
    ISSN (online) 1525-3198
    ISSN 0022-0302
    DOI 10.3168/jds.2018-15783
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Hepatitis A virus strains identified in jogaejeot associated with outbreaks in Seoul, South Korea

    Jeong, H.W / Kim, M.K / Yi, H.J / Kim, D.M / Jeon, S.J / Lee, H.K / Oh, Y.H / Hwang, Y.O

    Letters in applied microbiology. 2021 July, v. 73, no. 1

    2021  

    Abstract: Jogaejeot, seasoned Venerupis philippinarum, is a traditional Korean fermented food, and hepatitis A virus (HAV) can be transmitted through contaminated food, especially bivalve shellfish, causing acute gastroenteritis worldwide. Here, we carried out a ... ...

    Abstract Jogaejeot, seasoned Venerupis philippinarum, is a traditional Korean fermented food, and hepatitis A virus (HAV) can be transmitted through contaminated food, especially bivalve shellfish, causing acute gastroenteritis worldwide. Here, we carried out a phylogenetic analysis to identify and characterize HAV strains in jogaejeot samples associated with hepatitis A (HA) outbreaks in Seoul, South Korea, in 2019. The HAV strains were identified using blast and molecular analysis of the amplified HAV VP1‐P2B genome region. The HAV strains identified in the five jogaejeot samples shared at least 99% sequence identity, were all classified as genotype IA and were most closely related to strains that are widespread in East Asia. These results support a link between the consumption of jogaejeot and the HA outbreaks observed in 2019 in Seoul. In addition, they indicate a need for more stringent enforcement of food safety regulations for the shellfish industry, especially against HAV, and the value of widespread vaccination.
    Keywords Hepatovirus A ; Ruditapes philippinarum ; fermented foods ; food contamination ; gastroenteritis ; genome ; genotype ; hepatitis A ; industry ; microbiology ; phylogeny ; sequence analysis ; shellfish ; vaccination ; South Korea
    Language English
    Dates of publication 2021-07
    Size p. 107-112.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note NAL-AP-2-clean ; JOURNAL ARTICLE
    ZDB-ID 632584-1
    ISSN 1472-765X ; 0266-8254
    ISSN (online) 1472-765X
    ISSN 0266-8254
    DOI 10.1111/lam.13482
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  5. Article ; Online: Unraveling the immune and metabolic changes associated with metritis in dairy cows.

    Casaro, S / Prim, J G / Gonzalez, T D / Bisinotto, R S / Chebel, R C / Marrero, M G / Silva, A C M / Santos, J E P / Nelson, C D / Laporta, J / Jeon, S J / Bicalho, R C / Driver, J P / Galvão, K N

    Journal of dairy science

    2023  Volume 106, Issue 12, Page(s) 9244–9259

    Abstract: The objective was to unravel the peripartum immune and metabolic changes associated with metritis in Holstein cows. Holstein cows (n = 128) had blood collected at -14, 0, 3, and 7 d relative to parturition (DRP). Flow cytometry was used to evaluate blood ...

    Abstract The objective was to unravel the peripartum immune and metabolic changes associated with metritis in Holstein cows. Holstein cows (n = 128) had blood collected at -14, 0, 3, and 7 d relative to parturition (DRP). Flow cytometry was used to evaluate blood leukocyte counts, proportions, and activation. Total cells, live cells, single cells, monocytes (CD172α
    MeSH term(s) Female ; Cattle ; Animals ; CD8-Positive T-Lymphocytes ; Gas Chromatography-Mass Spectrometry/veterinary ; Postpartum Period ; Cytokines ; Inflammation/veterinary ; Pelvic Inflammatory Disease/veterinary ; Cattle Diseases ; Lactation
    Chemical Substances Cytokines
    Language English
    Publishing date 2023-08-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 242499-x
    ISSN 1525-3198 ; 0022-0302
    ISSN (online) 1525-3198
    ISSN 0022-0302
    DOI 10.3168/jds.2023-23289
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Age‐0 striped bass, Morone saxatilis (Walbaum, 1792), response to immunostimulation

    Jeon, S. J / Fast, M. D

    Journal of applied ichthyology. 2015 Jan., v. 31, no. 1

    2015  

    Abstract: Young‐of‐the‐year (age‐0) striped bass, Morone saxatilis, were studied to characterize their responses to inflammatory stimuli. There were two studies, with the hypotheses that (i) <5 g striped bass would respond to inflammatory insult within 6 h, and ( ... ...

    Abstract Young‐of‐the‐year (age‐0) striped bass, Morone saxatilis, were studied to characterize their responses to inflammatory stimuli. There were two studies, with the hypotheses that (i) <5 g striped bass would respond to inflammatory insult within 6 h, and (ii) response to antigens would be maintained for >24 h in larger fish. Study I was conducted to understand the cytokine expression pattern (IL‐1β, TNF‐α, Nramp and TGF‐β) in response to lipopolysaccharide (LPS) or Freund's complete adjuvant (FCA) stimulation in age‐0 striped bass (3.44 ± 1.68 g, 70.6 ± 10.3 mm fork length) up to 24 h post injection (hpi). Study II was similar to Study I, but striped bass were sampled over a longer time frame (by 48 hpi) and larger age‐0 striped bass were used (20.6 ± 5.9 g, 129.2 ± 10.9 mm fork length). It was confirmed that immunostimulants such as LPS and FCA induce production of inflammatory cytokines and Nramp, which are important in innate immune response to bacterial infection. The responses were rapidly stimulated with LPS (IL‐1β, TNF‐α, TGF‐β >3‐fold increase, compared to PBS) or FCA (IL‐1β >3‐fold and TGF‐β >2‐fold, compared to PBS) within 6 hpi and maintained in most cases 48 hpi (spleen Nramp and TGF‐β 2‐fold >PBS, at 24 and 48 h), similar to other teleosts. Intraperitoneal injection with PBS also simulated inflammatory gene expression, but was delayed (IL‐1β, TNF‐α, TGF‐β and Nramp, FCA and LPS< at 6 h; 24 h >LPS and PBS) in comparison to LPS and FCA, suggesting that this procedure and possibly the volume used can be stimulatory and potentially harmful in age‐0 fish. Therefore, this study suggests that age‐0 striped bass are capable of strong cytokine induction in response to immunological stimulation within a very short period of time.
    Keywords Morone saxatilis ; bacterial infections ; fish ; gene expression ; immune response ; immunostimulants ; inflammation ; innate immunity ; interleukin-1beta ; intraperitoneal injection ; lipopolysaccharides ; spleen ; transforming growth factor beta ; tumor necrosis factor-alpha
    Language English
    Dates of publication 2015-01
    Size p. 125-134.
    Publishing place Blackwell Wissenschafts-Verlag
    Document type Article
    ZDB-ID 283875-8
    ISSN 0175-8659
    ISSN 0175-8659
    DOI 10.1111/jai.12665
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Synovial chondromatosis in the temporomandibular joint: a case with typical imaging features and pathological findings.

    Lim, S W / Jeon, S J / Choi, S S / Choi, K H

    The British journal of radiology

    2011  Volume 84, Issue 1007, Page(s) e213–6

    Abstract: Synovial chondromatosis of the temporomandibular joint is a rare benign joint disorder that has been reported in only a few studies. However, we recently encountered a pathologically proven case of this disorder. This case also showed the typical imaging ...

    Abstract Synovial chondromatosis of the temporomandibular joint is a rare benign joint disorder that has been reported in only a few studies. However, we recently encountered a pathologically proven case of this disorder. This case also showed the typical imaging findings on panoramic radiographs and on CT and MR images. Therefore, we report this case and the imaging and pathological findings.
    MeSH term(s) Calcinosis/complications ; Calcinosis/diagnosis ; Chondromatosis, Synovial/complications ; Chondromatosis, Synovial/diagnosis ; Chondromatosis, Synovial/pathology ; Chondromatosis, Synovial/surgery ; Facial Pain/etiology ; Facial Pain/surgery ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Temporomandibular Joint Disorders/diagnosis ; Temporomandibular Joint Disorders/pathology ; Temporomandibular Joint Disorders/surgery ; Tomography, X-Ray Computed ; Treatment Outcome
    Language English
    Publishing date 2011-10-20
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 2982-8
    ISSN 1748-880X ; 0007-1285
    ISSN (online) 1748-880X
    ISSN 0007-1285
    DOI 10.1259/bjr/69067316
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  8. Article ; Online: The potential role of melatonin on sleep deprivation-induced cognitive impairments: implication of FMRP on cognitive function.

    Kwon, K J / Lee, E J / Kim, M K / Jeon, S J / Choi, Y Y / Shin, C Y / Han, S-H

    Neuroscience

    2015  Volume 301, Page(s) 403–414

    Abstract: While prolonged sleep deprivation (SD) could lead to profound negative health consequences, such as impairments in vital biological functions of immunity and cognition, melatonin possesses powerful ameliorating effects against those harmful insults. ... ...

    Abstract While prolonged sleep deprivation (SD) could lead to profound negative health consequences, such as impairments in vital biological functions of immunity and cognition, melatonin possesses powerful ameliorating effects against those harmful insults. Melatonin has strong antioxidant and anti-inflammatory effects that help to restore body's immune and cognitive functions. In this study, we investigated the possible role of melatonin in reversing cognitive dysfunction induced by SD in rats. Our experimental results revealed that sleep-deprived animals exhibited spatial memory impairment in the Morris water maze tasks compared with the control groups. Furthermore, there was an increased glial activation most prominent in the hippocampal region of the SD group compared to the normal control (NC) group. Additionally, markers of oxidative stress such as 4-hydroxynonenal (4-HNE) and 7,8-dihydro-8-oxo-deoxyguanine (8-oxo-dG) were significantly increased, while fragile X-mental retardation protein (FMRP) expression was decreased in the SD group. Interestingly, melatonin treatment normalized these events to control levels following SD. Our data demonstrate that SD induces oxidative stress through glial activation and decreases FMRP expression in the neurons. Furthermore, our results suggest the efficacy of melatonin for the treatment of sleep-related neuronal dysfunction, which occurs in neurological disorders such as Alzheimer's disease and autism.
    MeSH term(s) Aldehydes/metabolism ; Animals ; Antioxidants/therapeutic use ; Brain/drug effects ; Brain/metabolism ; Cell Survival/genetics ; Cells, Cultured ; Cerebral Cortex/cytology ; Deoxyguanosine/analogs & derivatives ; Deoxyguanosine/metabolism ; Disease Models, Animal ; Embryo, Mammalian ; Fragile X Mental Retardation Protein/genetics ; Fragile X Mental Retardation Protein/metabolism ; Gene Expression Regulation/drug effects ; Gene Expression Regulation/genetics ; Male ; Maze Learning/drug effects ; Melatonin/therapeutic use ; Memory Disorders/etiology ; Memory Disorders/prevention & control ; Neurons/drug effects ; Neurons/metabolism ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; Reactive Oxygen Species/metabolism ; Sleep Deprivation/complications
    Chemical Substances Aldehydes ; Antioxidants ; Fmr1 protein, rat ; RNA, Small Interfering ; Reactive Oxygen Species ; Fragile X Mental Retardation Protein (139135-51-6) ; 8-oxo-7-hydrodeoxyguanosine (88847-89-6) ; Deoxyguanosine (G9481N71RO) ; Melatonin (JL5DK93RCL) ; 4-hydroxy-2-nonenal (K1CVM13F96)
    Language English
    Publishing date 2015-08-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 196739-3
    ISSN 1873-7544 ; 0306-4522
    ISSN (online) 1873-7544
    ISSN 0306-4522
    DOI 10.1016/j.neuroscience.2015.05.079
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  9. Article: Nanowires array modified electrode for enhanced electrochemical detection of nucleic acid

    Ramulu, T.S / Venu, R / Sinha, B / Lim, B / Jeon, S.J / Yoon, S.S / Kim, C.G

    Biosensors & bioelectronics. 2013 Feb. 15, v. 40, no. 1

    2013  

    Abstract: The gold nanowires array electrode (AuNWsA) was synthesized by two step electrodeposition, which provided well oriented vertically aligned nanowires. The dimensions of the nanowires were determined by scanning electron micrograph and found to be around 1. ...

    Abstract The gold nanowires array electrode (AuNWsA) was synthesized by two step electrodeposition, which provided well oriented vertically aligned nanowires. The dimensions of the nanowires were determined by scanning electron micrograph and found to be around 1.5μm in length with 200nm diameter. Each nanowire was separated by a distance of 2–3 times the diameter of the nanowire itself. The electrochemical performance of the AuNWsA electrode was evaluated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Using these analytical tools, this AuNWsA electrode was shown to have a high effective surface area and excellent electron transfer surfaces compared with flat bare Au electrode. The AuNWsA electrode was then used as an electrochemical biosensor electrode by immobilizing probe DNA and analyzed by CV, EIS and Fourier transform infrared measurements. The results of this analysis suggested that the AuNWsA electrode provides good surfaces for the immobilization and hybridization of DNA. The selectivity of the probe DNA immobilized AuNWsA electrode was tested using non-complementary and one base pair mismatching DNA. The detection limit of the AuNWsA electrode was determined to be 6.78×10⁻⁹M, which is two times smaller than the bare Au electrode.
    Keywords DNA ; biosensors ; detection limit ; dielectric spectroscopy ; electrochemistry ; electrodes ; electron transfer ; gold ; nanowires ; nucleic acid hybridization ; surface area
    Language English
    Dates of publication 2013-0215
    Size p. 258-264.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2012.07.034
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  10. Article ; Online: Nanowires array modified electrode for enhanced electrochemical detection of nucleic acid.

    Ramulu, T S / Venu, R / Sinha, B / Lim, B / Jeon, S J / Yoon, S S / Kim, C G

    Biosensors & bioelectronics

    2013  Volume 40, Issue 1, Page(s) 258–264

    Abstract: The gold nanowires array electrode (AuNWsA) was synthesized by two step electrodeposition, which provided well oriented vertically aligned nanowires. The dimensions of the nanowires were determined by scanning electron micrograph and found to be around 1. ...

    Abstract The gold nanowires array electrode (AuNWsA) was synthesized by two step electrodeposition, which provided well oriented vertically aligned nanowires. The dimensions of the nanowires were determined by scanning electron micrograph and found to be around 1.5 μm in length with 200 nm diameter. Each nanowire was separated by a distance of 2-3 times the diameter of the nanowire itself. The electrochemical performance of the AuNWsA electrode was evaluated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Using these analytical tools, this AuNWsA electrode was shown to have a high effective surface area and excellent electron transfer surfaces compared with flat bare Au electrode. The AuNWsA electrode was then used as an electrochemical biosensor electrode by immobilizing probe DNA and analyzed by CV, EIS and Fourier transform infrared measurements. The results of this analysis suggested that the AuNWsA electrode provides good surfaces for the immobilization and hybridization of DNA. The selectivity of the probe DNA immobilized AuNWsA electrode was tested using non-complementary and one base pair mismatching DNA. The detection limit of the AuNWsA electrode was determined to be 6.78×10(-9) M, which is two times smaller than the bare Au electrode.
    MeSH term(s) Biosensing Techniques/instrumentation ; Conductometry/instrumentation ; DNA/genetics ; Electrodes ; Equipment Design ; Equipment Failure Analysis ; Nanotubes/chemistry ; Reproducibility of Results ; Sensitivity and Specificity ; Sequence Analysis, DNA/instrumentation
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2013-02-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2012.07.034
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