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Article ; Online: Perfil sociolingüístico de Frontera Corozal, Ocosingo, Chiapas

Juan Jesús Vázquez Álvarez

Revista Pueblos y Fronteras Digital, Vol 14, Iss - (2019)

2019

Abstract: Se presenta el panorama sociolingüístico de Frontera Corozal, un poblado chol fundado a partir de la migración de mediados del siglo pasado. Los choles de este lugar están en contacto lingüístico con lenguas dominantes extranjeras como el inglés y, desde ...

Abstract	Se presenta el panorama sociolingüístico de Frontera Corozal, un poblado chol fundado a partir de la migración de mediados del siglo pasado. Los choles de este lugar están en contacto lingüístico con lenguas dominantes extranjeras como el inglés y, desde luego, con el español, por su cercanía con los sitios arqueológicos de Yaxchilán y Bonampak. También mantienen una convivencia cercana con hablantes de otras lenguas indígenas como el tseltal y el lacandón. A pesar de la experiencia lingüística multilingüe en la comunidad y el deseo generalizado de que los hijos aprendan español, en este estudio se destaca que la mayoría de los niños tienen como lengua materna el chol, mientras que otros lo aprenden como segunda lengua. El habla de la población infantil y de algunos adultos de la comunidad de estudio dan indicios de una neutralización de la variación dialectal que caracteriza los lugares de origen de los colonos (chol de Tila vs chol de Tumbalá)
Keywords	choles ; migración ; variación dialectal ; actitudes lingüísticas ; transmisión intergeneracional ; Social Sciences ; H ; Social sciences (General) ; H1-99
Language	English
Publishing date	2019-11-01T00:00:00Z
Publisher	Universidad Nacional Autónoma de México
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: TurboPutative

Rafael Barrero-Rodríguez / Jose Manuel Rodriguez / Rocío Tarifa / Jesús Vázquez / Annalaura Mastrangelo / Alessia Ferrarini

Frontiers in Molecular Biosciences, Vol

A web server for data handling and metabolite classification in untargeted metabolomics

2022 Volume 9

Abstract: Untargeted metabolomics aims at measuring the entire set of metabolites in a wide range of biological samples. However, due to the high chemical diversity of metabolites that range from small to large and more complex molecules (i.e., amino acids/ ... ...

Abstract	Untargeted metabolomics aims at measuring the entire set of metabolites in a wide range of biological samples. However, due to the high chemical diversity of metabolites that range from small to large and more complex molecules (i.e., amino acids/carbohydrates vs. phospholipids/gangliosides), the identification and characterization of the metabolome remain a major bottleneck. The first step of this process consists of searching the experimental monoisotopic mass against databases, thus resulting in a highly redundant/complex list of candidates. Despite the progress in this area, researchers are still forced to manually explore the resulting table in order to prioritize the most likely identifications for further biological interpretation or confirmation with standards. Here, we present TurboPutative (https://proteomics.cnic.es/TurboPutative/), a flexible and user-friendly web-based platform composed of four modules (Tagger, REname, RowMerger, and TPMetrics) that streamlines data handling, classification, and interpretability of untargeted LC-MS-based metabolomics data. Tagger classifies the different compounds and provides preliminary insights into the biological system studied. REname improves putative annotation handling and visualization, allowing the recognition of isomers and equivalent compounds and redundant data removal. RowMerger reduces the dataset size, facilitating the manual comparison among annotations. Finally, TPMetrics combines different datasets with feature intensity and relevant information for the researcher and calculates a score based on adduct probability and feature correlations, facilitating further identification, assessment, and interpretation of the results. The TurboPutative web application allows researchers in the metabolomics field that are dealing with massive datasets containing multiple putative annotations to reduce the number of these entries by 80%–90%, thus facilitating the extrapolation of biological knowledge and improving metabolite prioritization for subsequent pathway ...
Keywords	putative annotations ; LC-MS ; simplification ; lipids ; metabolite ID prioritize ; Biology (General) ; QH301-705.5
Subject code	004
Language	English
Publishing date	2022-09-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Multi-Organ Increase in Norepinephrine Levels after Central Leptin Administration and Diet-Induced Obesity

Daniela Fernandois / María Jesús Vázquez / Alexia Barroso / Alfonso H. Paredes / Manuel Tena-Sempere / Gonzalo Cruz

International Journal of Molecular Sciences, Vol 24, Iss 23, p

2023 Volume 16909

Abstract: Autonomic innervation is important to regulate homeostasis in every organ of the body. The sympathetic nervous system controls several organs associated with metabolism and reproduction, including adipose tissue, the liver, and the ovaries. The ... ...

Abstract	Autonomic innervation is important to regulate homeostasis in every organ of the body. The sympathetic nervous system controls several organs associated with metabolism and reproduction, including adipose tissue, the liver, and the ovaries. The sympathetic nervous system is controlled within the central nervous system by neurons located in the hypothalamus, which in turn are regulated by hormones like leptin. Leptin action in the hypothalamus leads to increased sympathetic activity in the adipose tissue. In this short report, we propose that leptin action in the brain also controls the sympathetic innervation of other organs like the liver and the ovary. We performed two experiments: We performed an intracerebroventricular (ICV) injection of leptin and measured norepinephrine levels in several organs, and we used a validated model of overnutrition and obesity to evaluate whether an increase in leptin levels coexists with high levels of norepinephrine in the liver and ovaries. Norepinephrine was measured by ELISA in adipose tissue and by HPLC-EC in other tissues. Leptin was measured by ELISA. We found that the ICV injection of leptin increases norepinephrine levels in several organs, including the liver and ovaries. Also, we found that diet-induced obesity leads to an increase in leptin levels while inducing an increase in norepinephrine levels in the liver and ovaries. Finally, since hyperactivity of the sympathetic nervous system is observed both in non-alcoholic fatty liver disease and polycystic ovary syndrome, we think that an increase in norepinephrine levels induced by hyperleptinemia could be involved in the pathogenesis of both diseases.
Keywords	leptin ; norepinephrine ; sympathetic nervous system ; ovary ; liver ; fat ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	610
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Comparative proteomic analysis of nuclear and cytoplasmic compartments in human cardiac progenitor cells

Guillermo Albericio / Susana Aguilar / Jose Luis Torán / Rosa Yañez / Juan Antonio López / Jesús Vázquez / Carmen Mora / Antonio Bernad

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 16

Abstract: Abstract Clinical trials evaluating cardiac progenitor cells (CPC) demonstrated feasibility and safety, but no clear functional benefits. Therefore a deeper understanding of CPC biology is warranted to inform strategies capable to enhance their ... ...

Abstract	Abstract Clinical trials evaluating cardiac progenitor cells (CPC) demonstrated feasibility and safety, but no clear functional benefits. Therefore a deeper understanding of CPC biology is warranted to inform strategies capable to enhance their therapeutic potential. Here we have defined, using a label-free proteomic approach, the differential cytoplasmic and nuclear compartments of human CPC (hCPC). Global analysis of cytoplasmic repertoire in hCPC suggested an important hypoxia response capacity and active collagen metabolism. In addition, comparative analysis of the nuclear protein compartment identified a significant regulation of a small number of proteins in hCPC versus human mesenchymal stem cells (hMSC). Two proteins significantly upregulated in the hCPC nuclear compartment, IL1A and IMP3, showed also a parallel increase in mRNA expression in hCPC versus hMSC, and were studied further. IL1A, subjected to an important post-transcriptional regulation, was demonstrated to act as a dual-function cytokine with a plausible role in apoptosis regulation. The knockdown of the mRNA binding protein (IMP3) did not negatively impact hCPC viability, but reduced their proliferation and migration capacity. Analysis of a panel of putative candidate genes identified HMGA2 and PTPRF as IMP3 targets in hCPC. Therefore, they are potentially involved in hCPC proliferation/migration regulation.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Mapping the Serum Proteome of COVID-19 Patients; Guidance for Severity Assessment

Estefanía Nuñez / Irene Orera / Lorena Carmona-Rodríguez / José Ramón Paño / Jesús Vázquez / Fernando J. Corrales

Biomedicines, Vol 10, Iss 7, p

2022 Volume 1690

Abstract: Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), whose outbreak in 2019 led to an ongoing pandemic with devastating consequences for the global economy and human health. According to the World ...

Abstract	Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), whose outbreak in 2019 led to an ongoing pandemic with devastating consequences for the global economy and human health. According to the World Health Organization, COVID-19 has affected more than 481 million people worldwide, with 6 million confirmed deaths. The joint efforts of the scientific community have undoubtedly increased the pace of production of COVID-19 vaccines, but there is still so much uncharted ground to cover regarding the mechanisms of SARS-CoV-2 infection, replication and host response. These issues can be approached by proteomics with unprecedented capacity paving the way for the development of more efficient strategies for patient care. In this study, we present a deep proteome analysis that has been performed on a cohort of 72 COVID-19 patients aiming to identify serum proteins assessing the dynamics of the disease at different age ranges. A panel of 53 proteins that participate in several functions such as acute-phase response and inflammation, blood coagulation, cell adhesion, complement cascade, endocytosis, immune response, oxidative stress and tissue injury, have been correlated with patient severity, suggesting a molecular basis for their clinical stratification. Eighteen protein candidates were further validated by targeted proteomics in an independent cohort of 84 patients including a group of individuals that had satisfactorily resolved SARS-CoV-2 infection. Remarkably, all protein alterations were normalized 100 days after leaving the hospital, which further supports the reliability of the selected proteins as hallmarks of COVID-19 progression and grading. The optimized protein panel may prove its value for optimal severity assessment as well as in the follow up of COVID-19 patients.
Keywords	SARS-CoV-2 ; COVID-19 ; proteomics ; severity prognostics ; biomarkers ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: An analysis of tissue-specific alternative splicing at the protein level.

Jose Manuel Rodriguez / Fernando Pozo / Tomas di Domenico / Jesus Vazquez / Michael L Tress

PLoS Computational Biology, Vol 16, Iss 10, p e

2020 Volume 1008287

Abstract: The role of alternative splicing is one of the great unanswered questions in cellular biology. There is strong evidence for alternative splicing at the transcript level, and transcriptomics experiments show that many splice events are tissue specific. It ...

Abstract	The role of alternative splicing is one of the great unanswered questions in cellular biology. There is strong evidence for alternative splicing at the transcript level, and transcriptomics experiments show that many splice events are tissue specific. It has been suggested that alternative splicing evolved in order to remodel tissue-specific protein-protein networks. Here we investigated the evidence for tissue-specific splicing among splice isoforms detected in a large-scale proteomics analysis. Although the data supporting alternative splicing is limited at the protein level, clear patterns emerged among the small numbers of alternative splice events that we could detect in the proteomics data. More than a third of these splice events were tissue-specific and most were ancient: over 95% of splice events that were tissue-specific in both proteomics and RNAseq analyses evolved prior to the ancestors of lobe-finned fish, at least 400 million years ago. By way of contrast, three in four alternative exons in the human gene set arose in the primate lineage, so our results cannot be extrapolated to the whole genome. Tissue-specific alternative protein forms in the proteomics analysis were particularly abundant in nervous and muscle tissues and their genes had roles related to the cytoskeleton and either the structure of muscle fibres or cell-cell connections. Our results suggest that this conserved tissue-specific alternative splicing may have played a role in the development of the vertebrate brain and heart.
Keywords	Biology (General) ; QH301-705.5
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Capillary pruning couples tissue perfusion and oxygenation with cardiomyocyte maturation in the postnatal mouse heart

Ricardo Santamaría / Javier Cruz-Caballero / Polyxeni Gkontra / Alberto Jiménez-Montiel / Cristina Clemente / Juan A. López / María Villalba-Orero / Jesús Vázquez / Andreas Hutloff / Enrique Lara-Pezzi / Alicia G. Arroyo

Frontiers in Cell and Developmental Biology, Vol

2023 Volume 11

Abstract: Introduction: Removal of poorly perfused capillaries by pruning contributes to remodeling the microvasculature to optimize oxygen and nutrient delivery. Blood flow drives this process by promoting the intravascular migration of endothelial cells in ... ...

Abstract	Introduction: Removal of poorly perfused capillaries by pruning contributes to remodeling the microvasculature to optimize oxygen and nutrient delivery. Blood flow drives this process by promoting the intravascular migration of endothelial cells in developing networks, such as in the yolk sac, zebrafish brain or postnatal mouse retina.Methods: In this study, we have implemented innovative tools to recognize capillary pruning in the complex 3D coronary microvasculature of the postnatal mouse heart. We have also experimentally tested the impact of decreasing pruning on the structure and function of this network by altering blood flow with two different vasodilators: losartan and prazosin.Results: Although both drugs reduced capillary pruning, a combination of experiments based on ex vivo imaging, proteomics, electron microscopy and in vivo functional approaches showed that losartan treatment resulted in an inefficient coronary network, reduced myocardial oxygenation and metabolic changes that delayed the arrest of cardiomyocyte proliferation, in contrast to the effects of prazosin, probably due to its concomitant promotion of capillary expansion.Discussion: Our work demonstrates that capillary pruning contributes to proper maturation and function of the heart and that manipulation of blood flow may be a novel strategy to refine the microvasculature and improve tissue perfusion after damage.
Keywords	capillary pruning ; postnatal heart ; vasodilators ; blood flow ; oxygenation ; metabolism ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Immune synapse formation promotes lipid peroxidation and MHC-I upregulation in licensed dendritic cells for efficient priming of CD8+ T cells

Diego Calzada-Fraile / Salvador Iborra / Marta Ramírez-Huesca / Inmaculada Jorge / Enrico Dotta / Elena Hernández-García / Noa Martín-Cófreces / Estanislao Nistal-Villán / Esteban Veiga / Jesús Vázquez / Giulia Pasqual / Francisco Sánchez-Madrid

Nature Communications, Vol 14, Iss 1, Pp 1-

2023 Volume 16

Abstract: Abstract Antigen cognate dendritic cell (DC)-T cell synaptic interactions drive activation of T cells and instruct DCs. Upon receiving CD4+ T cell help, post-synaptic DCs (psDCs) are licensed to generate CD8+ T cell responses. However, the cellular and ... ...

Abstract	Abstract Antigen cognate dendritic cell (DC)-T cell synaptic interactions drive activation of T cells and instruct DCs. Upon receiving CD4+ T cell help, post-synaptic DCs (psDCs) are licensed to generate CD8+ T cell responses. However, the cellular and molecular mechanisms that enable psDCs licensing remain unclear. Here, we describe that antigen presentation induces an upregulation of MHC-I protein molecules and increased lipid peroxidation on psDCs in vitro and in vivo. We also show that these events mediate DC licensing. In addition, psDC adoptive transfer enhances pathogen-specific CD8+ T responses and protects mice from infection in a CD8+ T cell-dependent manner. Conversely, depletion of psDCs in vivo abrogates antigen-specific CD8+ T cell responses during immunization. Together, our data show that psDCs enable CD8+ T cell responses in vivo during vaccination and reveal crucial molecular events underlying psDC licensing.
Keywords	Science ; Q
Subject code	570
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Collective of Speech Therapists

Jesús Vazquez Campos / Julio Antonio Conill Armenteros / Rosario de la Caridad Sotolongo Peña

Revista de Ciencias Médicas de Pinar del Río, Vol 23, Iss 3, Pp 418-

a view from the initial and permanent training of these specialists

2019 Volume 426

Abstract: Introduction: the implementation of the collective of speech therapists is a necessity in order to take actions to the opening of the courses of studies and continuing training of professionals in the Logopedics studies. Objective: to analyze the ... ...

Abstract	Introduction: the implementation of the collective of speech therapists is a necessity in order to take actions to the opening of the courses of studies and continuing training of professionals in the Logopedics studies. Objective: to analyze the significance of the speech therapists collective for the opening of the study courses and continuing training of these specialists. Methods: a pedagogical study in which theoretical, empirical and statistical methods were used to collect, interpret and process information related to the subject. Results: the study revealed the significance of the speech-language therapists collective for the initial and permanent training of the speech-language specialist through the accomplishment of the following functions: organizational, teaching-methodological, development and research, which favors the improvement of the mother tongue, prevention, diagnosis and comprehensive speech-language along with communication disorders. Conclusions: a didactic-methodological strategy was implemented that identifies the speech therapists collective as a subsystem or basic organizational level of the specialty.
Keywords	comunicación ; estrategia ; lenguaje ; capacitación profesional ; trastornos ; estudiantes ; Medicine ; R ; Medicine (General) ; R5-920
Subject code	370
Language	Spanish
Publishing date	2019-05-01T00:00:00Z
Publisher	ECIMED
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Exogenous aralar/slc25a12 can replace citrin/slc25a13 as malate aspartate shuttle component in liver

Luis González-Moreno / Andrea Santamaría-Cano / Alberto Paradela / María Luz Martínez-Chantar / Miguel Á. Martín / Mercedes Pérez-Carreras / Alberto García-Picazo / Jesús Vázquez / Enrique Calvo / Gloria González-Aseguinolaza / Takeyori Saheki / Araceli del Arco / Jorgina Satrústegui / Laura Contreras

Molecular Genetics and Metabolism Reports, Vol 35, Iss , Pp 100967- (2023)

2023

Abstract: The deficiency of CITRIN, the liver mitochondrial aspartate–glutamate carrier (AGC), is the cause of four human clinical phenotypes, neonatal intrahepatic cholestasis caused by CITRIN deficiency (NICCD), silent period, failure to thrive and dyslipidemia ... ...

Abstract	The deficiency of CITRIN, the liver mitochondrial aspartate–glutamate carrier (AGC), is the cause of four human clinical phenotypes, neonatal intrahepatic cholestasis caused by CITRIN deficiency (NICCD), silent period, failure to thrive and dyslipidemia caused by CITRIN deficiency (FTTDCD), and citrullinemia type II (CTLN2). Clinical symptoms can be traced back to disruption of the malate-aspartate shuttle due to the lack of citrin. A potential therapy for this condition is the expression of aralar, the AGC present in brain, to replace citrin. To explore this possibility we have first verified that the NADH/NAD+ ratio increases in hepatocytes from citrin(−/−) mice, and then found that exogenous aralar expression reversed the increase in NADH/NAD+ observed in these cells. Liver mitochondria from citrin (−/−) mice expressing liver specific transgenic aralar had a small (~ 4–6 nmoles x mg prot−1 x min−1) but consistent increase in malate aspartate shuttle (MAS) activity over that of citrin(−/−) mice. These results support the functional replacement between AGCs in the liver. To explore the significance of AGC replacement in human therapy we studied the relative levels of citrin and aralar in mouse and human liver through absolute quantification proteomics. We report that mouse liver has relatively high aralar levels (citrin/aralar molar ratio of 7.8), whereas human liver is virtually devoid of aralar (CITRIN/ARALAR ratio of 397). This large difference in endogenous aralar levels partly explains the high residual MAS activity in liver of citrin(−/−) mice and why they fail to recapitulate the human disease, but supports the benefit of increasing aralar expression to improve the redox balance capacity of human liver, as an effective therapy for CITRIN deficiency.
Keywords	Mitochondria ; Citrin deficiency ; Aspartate-glutamate carrier ; Malate-aspartate shuttle ; Hepatocyte ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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