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Article ; Online: Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy.

Jevtic, Visnja / Kindle, Petra / Avilov, Sergiy V

Journal of visualized experiments : JoVE

2020 , Issue 160

Abstract: Mitochondrial nucleoids are compact particles formed by mitochondrial DNA molecules coated with proteins. Mitochondrial DNA encodes tRNAs, rRNAs, and several essential mitochondrial polypeptides. Mitochondrial nucleoids divide and distribute within the ... ...

Abstract	Mitochondrial nucleoids are compact particles formed by mitochondrial DNA molecules coated with proteins. Mitochondrial DNA encodes tRNAs, rRNAs, and several essential mitochondrial polypeptides. Mitochondrial nucleoids divide and distribute within the dynamic mitochondrial network that undergoes fission/fusion and other morphological changes. High resolution live fluorescence microscopy is a straightforward technique to characterize a nucleoid's position and motion. For this technique, nucleoids are commonly labeled through fluorescent tags of their protein components, namely transcription factor a (TFAM). However, this strategy needs overexpression of a fluorescent protein-tagged construct, which may cause artifacts (reported for TFAM), and is not feasible in many cases. Organic DNA-binding dyes do not have these disadvantages. However, they always show staining of both nuclear and mitochondrial DNAs, thus lacking specificity to mitochondrial nucleoids. By taking into account the physico-chemical properties of such dyes, we selected a nucleic acid gel stain (SYBR Gold) and achieved preferential labeling of mitochondrial nucleoids in live cells. Properties of the dye, particularly its high brightness upon binding to DNA, permit subsequent quantification of mitochondrial nucleoid motion using time series of super-resolution structured illumination images.
MeSH term(s)	Humans ; Microscopy, Fluorescence/methods ; Mitochondrial Dynamics/physiology ; Mitochondrial Proteins/metabolism
Chemical Substances	Mitochondrial Proteins
Language	English
Publishing date	2020-06-04
Publishing country	United States
Document type	Journal Article ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/60003
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Specific labeling of mitochondrial nucleoids for time-lapse structured illumination microscopy

Jevtic, Visnja / Kindle, Petra / Avilov, Sergiy V

Journal of visualized experiments. 2020 June 04, , no. 160

2020

Abstract	Mitochondrial nucleoids are compact particles formed by mitochondrial DNA molecules coated with proteins. Mitochondrial DNA encodes tRNAs, rRNAs, and several essential mitochondrial polypeptides. Mitochondrial nucleoids divide and distribute within the dynamic mitochondrial network that undergoes fission/fusion and other morphological changes. High resolution live fluorescence microscopy is a straightforward technique to characterize a nucleoid's position and motion. For this technique, nucleoids are commonly labeled through fluorescent tags of their protein components, namely transcription factor a (TFAM). However, this strategy needs overexpression of a fluorescent protein-tagged construct, which may cause artifacts (reported for TFAM), and is not feasible in many cases. Organic DNA-binding dyes do not have these disadvantages. However, they always show staining of both nuclear and mitochondrial DNAs, thus lacking specificity to mitochondrial nucleoids. By taking into account the physico-chemical properties of such dyes, we selected a nucleic acid gel stain (SYBR Gold) and achieved preferential labeling of mitochondrial nucleoids in live cells. Properties of the dye, particularly its high brightness upon binding to DNA, permit subsequent quantification of mitochondrial nucleoid motion using time series of super-resolution structured illumination images.
Keywords	dyes ; fluorescence ; fluorescence microscopy ; gels ; lighting ; mitochondria ; mitochondrial DNA ; physicochemical properties ; polypeptides ; ribosomal RNA ; staining ; time series analysis ; transcription factors ; transfer RNA
Language	English
Dates of publication	2020-0604
Size	p. e60003.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/60003
Database	NAL-Catalogue (AGRICOLA)

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Article: Imaging of renal osteodystrophy.

Jevtic, V

European journal of radiology

2003 Volume 46, Issue 2, Page(s) 85–95

Abstract: Chronic renal insufficiency, hemodialysis, peritoneal dialysis, renal transplantation and administration of different medications provoke complex biochemical disturbances of the calcium-phosphate metabolism with wide spectrum of bone and soft tissue ... ...

Abstract	Chronic renal insufficiency, hemodialysis, peritoneal dialysis, renal transplantation and administration of different medications provoke complex biochemical disturbances of the calcium-phosphate metabolism with wide spectrum of bone and soft tissue abnormalities termed renal osteodystrophy. Clinically most important manifestation of renal bone disease includes secondary hyperparathyroidism, osteomalacia/rickets, osteoporosis, adynamic bone disease and soft tissue calcification. As a complication of long-term hemodialysis and renal transplantation amyloid deposition, destructive spondyloarthropathy, osteonecrosis, and musculoskeletal infections may occur. Due to more sophisticated diagnostic methods and more efficient treatment classical radiographic features of secondary hyperparathyroidism and osteomalacia/rickets are now less frequently seen. Radiological investigations play an important role in early diagnosis and follow-up of the renal bone disease. Although numerous new imaging modalities have been introduced in clinical practice (scintigraphy, CT, MRI, quantitative imaging), plain film radiography, especially fine quality hand radiograph, still represents most widely used examination.
MeSH term(s)	Chronic Kidney Disease-Mineral and Bone Disorder/complications ; Chronic Kidney Disease-Mineral and Bone Disorder/diagnostic imaging ; Chronic Kidney Disease-Mineral and Bone Disorder/physiopathology ; Hand/diagnostic imaging ; Humans ; Hyperparathyroidism/diagnostic imaging ; Kidney Transplantation/adverse effects ; Osteomalacia/diagnostic imaging ; Osteonecrosis/diagnostic imaging ; Osteonecrosis/etiology ; Osteosclerosis/diagnostic imaging ; Osteosclerosis/etiology ; Radiography ; Renal Dialysis/adverse effects ; Skull/diagnostic imaging
Language	English
Publishing date	2003-05
Publishing country	Ireland
Document type	Journal Article ; Review
ZDB-ID	138815-0
ISSN	1872-7727 ; 0720-048X
ISSN (online)	1872-7727
ISSN	0720-048X
DOI	10.1016/s0720-048x(03)00072-x
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Zs.A 1630: Show issues

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Article ; Online: Differential diagnosis of rheumatoid and psoriatic arthritis at an early stage in the small hand and foot joints using magnetic resonance imaging.

Jevtic, V / Lingg, G

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...

2012 Volume 44, Issue 3, Page(s) 163–170

Abstract: The 2 major and clinically most important primary inflammatory rheumatic diseases which affect small hand and feet joints are rheumatoid arthritis (RA) and psoriatic arthritis (PsA). The most important initial histopathological feature of RA is synovitis ...

Abstract	The 2 major and clinically most important primary inflammatory rheumatic diseases which affect small hand and feet joints are rheumatoid arthritis (RA) and psoriatic arthritis (PsA). The most important initial histopathological feature of RA is synovitis followed by chronic proliferative granulomatous pannus-tissue, which is associated with cartilage and bone destruction. Early inflammatory changes in RA also develop synchronously within the subchondral bone marrow. Enthesitis is the hallmark of SNSA, and is often seen as one of the first radiological manifestations of the diseases. As a rule inflammation within the synovial joints, histologically similar to RA, is not so pronounced. Consequently destructive changes within the synovial joints are much less with the exception of PsA in which pronounced bone destruction may develop (arthritis mutilans). Considerable overlapping in clinical and morphological manifestation of RA and PsA may be present. For evaluation of hand and feet joints and surrounding soft tissue structures in RA and PsA different imaging modalities are used, which include projection radiography, ultrasonography (US), radionuclide techniques and magnetic resonance imaging (MRI). MRI has become the imaging modality of choice for evaluation of arthritis, when conventional radiography is not conclusive.
MeSH term(s)	Arthritis, Psoriatic/diagnosis ; Arthritis, Rheumatoid/diagnosis ; Contrast Media/administration & dosage ; Diagnosis, Differential ; Foot Joints/pathology ; Gadolinium DTPA ; Hand Joints/pathology ; Humans ; Image Enhancement/methods ; Image Interpretation, Computer-Assisted/methods ; Magnetic Resonance Imaging/methods ; Sensitivity and Specificity ; Synovitis/diagnosis ; Tenosynovitis/diagnosis
Chemical Substances	Contrast Media ; Gadolinium DTPA (K2I13DR72L)
Language	English
Publishing date	2012-06
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	392414-2
ISSN	1439-3980 ; 0722-1819
ISSN (online)	1439-3980
ISSN	0722-1819
DOI	10.1055/s-0032-1321760
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Zs.A 670: Show issues

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Article: Magnetic resonance imaging appearances of different discovertebral lesions.

Jevtic, V

European radiology

2001 Volume 11, Issue 7, Page(s) 1123–1135

Abstract: Magnetic resonance imaging (MRI) is not only an excellent imaging modality for the demonstration of morphological changes but is also capable of providing pathophysiological and pathoanatomic information about various spinal diseases. Different ... ...

Abstract	Magnetic resonance imaging (MRI) is not only an excellent imaging modality for the demonstration of morphological changes but is also capable of providing pathophysiological and pathoanatomic information about various spinal diseases. Different techniques offer opportunities to demonstrate the degree of water content, the vascularity of tissue components, the accumulation of fat, and new bone production. Thus MRI closely reflects the initial phase as well as the progression of pathoanatomic changes during the evolution of a disease. Due to the high sensitivity of MRI, abnormalities are often established at an early stage of discovertebral disease, when etiological diagnosis may be difficult. The specificity of MRI findings lags behind its sensitivity; similar changes can be demonstrated in etiologically different disease entities, which reflects the limited reactive possibilities of the osteoarticular system. In fact, the MRI morphological and signal intensity features of different discovertebral lesions are commonly determined more by their location and by the reactive capabilities of disc and bone than by their etiology. Early and exact MRI differentiation of various discovertebral lesions is of the utmost clinical importance for prompt institution of appropriate therapy.
MeSH term(s)	Cartilage/pathology ; Diagnosis, Differential ; Humans ; Intervertebral Disc/pathology ; Magnetic Resonance Imaging/methods ; Spinal Diseases/pathology ; Spine/pathology
Language	English
Publishing date	2001
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	1085366-2
ISSN	1432-1084 ; 0938-7994 ; 1613-3749
ISSN (online)	1432-1084
ISSN	0938-7994 ; 1613-3749
DOI	10.1007/s003300000727
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Zs.A 3300: Show issues

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Article ; Online: SYBR Gold dye enables preferential labelling of mitochondrial nucleoids and their time-lapse imaging by structured illumination microscopy.

Jevtic, Visnja / Kindle, Petra / Avilov, Sergiy V

PloS one

2018 Volume 13, Issue 9, Page(s) e0203956

Abstract: Mitochondrial DNA molecules coated with proteins form compact particles called mitochondrial nucleoids. They are redistributed within mitochondrial network undergoing morphological changes. The straightforward technique to characterize nucleoids' motions ...

Abstract	Mitochondrial DNA molecules coated with proteins form compact particles called mitochondrial nucleoids. They are redistributed within mitochondrial network undergoing morphological changes. The straightforward technique to characterize nucleoids' motions is fluorescence microscopy. Mitochondrial nucleoids are commonly labelled with fluorescent protein tags, which is not always feasible and was reported to cause artifacts. Organic DNA-binding dyes are free of these drawbacks, but they lack specificity to mitochondrial DNA. Here, considering physico-chemical properties of such dyes, we achieved preferential live-cell labelling of mitochondrial nucleoids by a nucleic acid staining dye SYBR Gold. It enabled time-lapse imaging of mitochondrial nucleoids by structured illumination microscopy and quantification of their motions.
MeSH term(s)	A549 Cells ; Animals ; Cercopithecus aethiops ; Coloring Agents/metabolism ; DNA, Mitochondrial/metabolism ; DNA, Mitochondrial/ultrastructure ; HeLa Cells ; Humans ; Microscopy/methods ; Mitochondria/metabolism ; Mitochondria/ultrastructure ; Organic Chemicals/metabolism ; Time-Lapse Imaging ; Vero Cells
Chemical Substances	Coloring Agents ; DNA, Mitochondrial ; Organic Chemicals ; SYBR Gold nucleic acid gel stain
Language	English
Publishing date	2018-09-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0203956
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Article: Differential Diagnosis of Rheumatoid and Psoriatic Arthritis at an Early Stage in the Small Hand and Foot Joints using Magnetic Resonance Imaging

Jevtic, V. / Lingg, G.

Aktuelle Rheumatologie

2012 Volume 37, Issue 02, Page(s) 105–112

Abstract	The 2 major and clinically most important primary inflammatory rheumatic diseases which affect small hand and feet joints are rheumatoid arthritis (RA) and psoriatic arthritis (PsA). The most important initial histopathological feature of RA is synovitis followed by chronic proliferative granulomatous pannus-tissue, which is associated with cartilage and bone destruction. Early inflammatory changes in RA also develop synchronously within the subchondral bone marrow. Enthesitis is the hallmark of SNSA, and is often seen as one of the first radiological manifestations of the diseases. As a rule inflammation within the synovial joints, histologically similar to RA, is not so pronounced. Consequently destructive changes within the synovial joints are much less with the exception of PsA in which pronounced bone destruction may develop (arthritis mutilans). Considerable overlapping in clinical and morphological manifestation of RA and PsA may be present. For evaluation of hand and feet joints and surrounding soft tissue structures in RA and PsA different imaging modalities are used, which include projection radiography, ultrasonography (US), radionuclide techniques and magnetic resonance imaging (MRI). MRI has become the imaging modality of choice for evaluation of arthritis, when conventional radiography is not conclusive.
Keywords	rheumatoid arthritis ; psoriatic arthritis ; small joints of the hand and feet ; differential diagnosis ; magnetic resonance imaging ; Rheumatoide Arthritis ; Psoriasis-arthritis ; kleine Gelenke der Hände und Vorfüsse ; Differentialdiagnose ; MRT
Language	English
Publishing date	2012-04-01
Publisher	© Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	131267-4
ISSN	1438-9940 ; 0341-051X
ISSN (online)	1438-9940
ISSN	0341-051X
DOI	10.1055/s-0032-1301929
Database	Thieme publisher's database

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Zs.A 1289: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Differential Diagnosis of Rheumatoid and Psoriatic Arthritis at an Early Stage in the Small Hand and Foot Joints using Magnetic Resonance Imaging. Differenzialdiagnose zwischen Rheumatoider und Psoriasis-Arthritis im Frühstadium an den kleinen Gelenken der Hände und Vorfüsse mittels MRT

Jevtic, V. / Lingg, G.

Aktuelle Rheumatologie

2012 Volume 37, Issue 2, Page(s) 105

Language	German
Document type	Article
ZDB-ID	131267-4
ISSN	0341-051X
Database	Current Contents Medicine

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Zs.A 1289: Show issues

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Article: Kernspintomografische Differenzialdiagnose unterschiedlicher discovertebraler Läsionen. Differential Diagnosis of Various Discovertebral Lesions by Magnetic Resonance Imaging

Lingg, G. / Jevtic, V.

Aktuelle Rheumatologie

2012 Volume 37, Issue 2, Page(s) 113

Language	German
Document type	Article
ZDB-ID	131267-4
ISSN	0341-051X
Database	Current Contents Medicine

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Zs.A 1289: Show issues

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Article: Kernspintomografische Differenzialdiagnose unterschiedlicher discovertebraler Läsionen

Lingg, G. / Jevtic, V.

Aktuelle Rheumatologie

2012 Volume 37, Issue 02, Page(s) 113–118

Abstract: Die Kernspintomografie ist bei sehr unterschiedlichen diskovertebralen Läsionen nicht nur eine exzellente Methode, um die makromorphologischen Veränderungen aufzuzeigen, sondern sie liefert auch pathophysiologische und pathoanatomische Informationen über ...

Abstract	Die Kernspintomografie ist bei sehr unterschiedlichen diskovertebralen Läsionen nicht nur eine exzellente Methode, um die makromorphologischen Veränderungen aufzuzeigen, sondern sie liefert auch pathophysiologische und pathoanatomische Informationen über diese Erkrankungen. Mit den verschiedenen Sequenzen lässt sich der Wassergehalt, die Vaskularisation von Geweben, die Umwandlung von hämatopoetischem in Fettmark und die Knochenneubildung aufzeigen. Auf diese Art und Weise kann die Kernspintomografie sowohl das Anfangsstadium, als auch die Progredienz und die Heilung einer Erkrankung wiederspiegeln. Dabei ist ihre Sensitivität höher als ihre Spezifität. Aufgrund des begrenzten Reaktionsspektrums der beteiligten Gewebe des Bewegungsapparats kann es bei unterschiedlichen Entitäten zu ganz ähnlichen MRT-Erscheinungsbildern kommen. Möglichkeiten, dann doch eine differenzialdiagnostische Entscheidung zu treffen werden aufgezeigt. Dies kann im Einzelfall von höchster klinischer Bedeutung sein, um ein unmittelbares therapeutisches Eingreifen zu ermöglichen.
Keywords	MRT ; infektiöse „Spondylodiscitis“ ; rheumatische Spondylodiscitis ; erosive Osteochondrose ; MRI ; infectious spondylodiscitis ; rheumatic spondylodiscitis ; erosive osteochondrosis
Language	German
Publishing date	2012-04-01
Publisher	© Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	131267-4
ISSN	1438-9940 ; 0341-051X
ISSN (online)	1438-9940
ISSN	0341-051X
DOI	10.1055/s-0032-1309000
Database	Thieme publisher's database

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Zs.A 1289: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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