Article ; Online: Overexpression of miR-98 inhibits cell invasion in glioma cell lines via downregulation of IKKε.
European review for medical and pharmacological sciences
2015 Volume 19, Issue 19, Page(s) 3593–3604
Abstract: Objective: MicroRNAs (miRNAs) function as negative regulators for the expression of genes involved in cancer metastasis. The aim of this study was to investigate the potential role of miR-98 in gliomas and validate its regulatory mechanism.: Patients ... ...
| Abstract | Objective: MicroRNAs (miRNAs) function as negative regulators for the expression of genes involved in cancer metastasis. The aim of this study was to investigate the potential role of miR-98 in gliomas and validate its regulatory mechanism. Patients and methods: Cell viability assays are used to measure proliferation of cell. mRNA expression is measured by qRT-PCR. Western blot analysis is used to measure protein expression. Results: Functional studies showed that miR-98 overexpression inhibited glioma migration and invasion, but had no effect on the cell viability. An enhanced green fluorescent protein reporter assay, quantitative RT-PCR, and a western blot analysis confirmed that miR-98 suppressed the expression of IκB kinase (IKKε) by directly targeting its 3'-untranslated region, also, the NF-κB p65 nuclear translocation and matrix metalloproteinase (MMP)-9 expression were significantly arrested in glioma cells treated with miR-98 mimics. Accordingly, the overexpression of IKKε or NF-κB p65 can restore cell migration and invasion after being inhibited by miR-98, and can restore NF-κB p65 nuclear translocation as well as increase MMP-9 expression. Conclusions: These findings demonstrated that miR-98 functions as a tumor suppressor in gliomas. Furthermore, miR-98 may act as a potential therapeutic biomarker for glioma patients. |
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| MeSH term(s) | Adult ; Brain Neoplasms/genetics ; Brain Neoplasms/metabolism ; Brain Neoplasms/pathology ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Survival/genetics ; Down-Regulation/physiology ; Female ; Gene Expression Regulation, Neoplastic ; Glioma/genetics ; Glioma/metabolism ; Glioma/pathology ; Humans ; I-kappa B Kinase/genetics ; I-kappa B Kinase/metabolism ; Male ; MicroRNAs/biosynthesis ; MicroRNAs/genetics ; Middle Aged ; Neoplasm Invasiveness/genetics | |||||
| Chemical Substances | MIRN98 microRNA, human ; MicroRNAs ; I-kappa B Kinase (EC 2.7.11.10) | |||||
| Language | English | |||||
| Publishing date | 2015-10 | |||||
| Publishing country | Italy | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 605550-3 | |||||
| ISSN | 2284-0729 ; 1128-3602 ; 0392-291X | |||||
| ISSN (online) | 2284-0729 | |||||
| ISSN | 1128-3602 ; 0392-291X | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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