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  1. Article ; Online: Biomaterial-based mechanical regulation facilitates scarless wound healing with functional skin appendage regeneration.

    Li, Ying-Ying / Ji, Shuai-Fei / Fu, Xiao-Bing / Jiang, Yu-Feng / Sun, Xiao-Yan

    Military Medical Research

    2024  Volume 11, Issue 1, Page(s) 13

    Abstract: Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages, ultimately impairing its normal physiological function. Accumulating evidence underscores ... ...

    Abstract Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages, ultimately impairing its normal physiological function. Accumulating evidence underscores the potential of targeted modulation of mechanical cues to enhance skin regeneration, promoting scarless repair by influencing the extracellular microenvironment and driving the phenotypic transitions. The field of skin repair and skin appendage regeneration has witnessed remarkable advancements in the utilization of biomaterials with distinct physical properties. However, a comprehensive understanding of the underlying mechanisms remains somewhat elusive, limiting the broader application of these innovations. In this review, we present two promising biomaterial-based mechanical approaches aimed at bolstering the regenerative capacity of compromised skin. The first approach involves leveraging biomaterials with specific biophysical properties to create an optimal scarless environment that supports cellular activities essential for regeneration. The second approach centers on harnessing mechanical forces exerted by biomaterials to enhance cellular plasticity, facilitating efficient cellular reprogramming and, consequently, promoting the regeneration of skin appendages. In summary, the manipulation of mechanical cues using biomaterial-based strategies holds significant promise as a supplementary approach for achieving scarless wound healing, coupled with the restoration of multiple skin appendage functions.
    MeSH term(s) Humans ; Wound Healing/physiology ; Biocompatible Materials/therapeutic use ; Biocompatible Materials/chemistry ; Skin/injuries ; Cicatrix/pathology
    Chemical Substances Biocompatible Materials
    Language English
    Publishing date 2024-02-18
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2768940-2
    ISSN 2054-9369 ; 2054-9369
    ISSN (online) 2054-9369
    ISSN 2054-9369
    DOI 10.1186/s40779-024-00519-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Human urinary cells for functional wound healing with sweat gland restoration.

    Ji, Shuai-Fei / Zhou, Lai-Xian / Li, Ying-Ying / Xiang, Jiang-Bing / Chen, Hua-Ting / Liu, Yi-Qiong / Fu, Xiao-Bing / Sun, Xiao-Yan

    Military Medical Research

    2023  Volume 10, Issue 1, Page(s) 57

    MeSH term(s) Humans ; Sweat Glands ; Wound Healing/physiology
    Language English
    Publishing date 2023-11-28
    Publishing country England
    Document type Letter
    ZDB-ID 2768940-2
    ISSN 2054-9369 ; 2054-9369
    ISSN (online) 2054-9369
    ISSN 2054-9369
    DOI 10.1186/s40779-023-00492-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Protective effect of ginsenoside Rd on military aviation noise-induced cochlear hair cell damage in guinea pigs

    Chen, Xue-min / Liu, Yuhui / Ji, Shuai-fei / Xue, Xin-miao / Wang, Linlin / Zhang, Min / Chang, Yao-ming / Wang, Xiao-cheng

    Environ Sci Pollut Res. 2023 Feb., v. 30, no. 9 p.23965-23981

    2023  

    Abstract: Noise pollution has become one of the important social hazards that endanger the auditory system of residents, causing noise-induced hearing loss (NIHL). Oxidative stress has a significant role in the pathogenesis of NIHL, in which the silent information ...

    Abstract Noise pollution has become one of the important social hazards that endanger the auditory system of residents, causing noise-induced hearing loss (NIHL). Oxidative stress has a significant role in the pathogenesis of NIHL, in which the silent information regulator 1(SIRT1)/proliferator-activated receptor-gamma coactivator 1α (PGC-1α) signaling pathway is closely engaged. Ginsenoside Rd (GSRd), a main monomer extract from ginseng plants, has been confirmed to suppress oxidative stress. Therefore, the hypothesis that GSRd may attenuate noise-induced cochlear hair cell loss seemed promising. Forty-eight male guinea pigs were randomly divided into four groups: control, noise exposure, GSRd treatment (30 mg/kg Rd for 10d + noise), and experimental control (30 mg/kg glycerol + noise). The experimental groups received military helicopter noise exposure at 115 dB (A) for 4 h daily for five consecutive days. Hair cell damage was evaluated by using inner ear basilar membrane preparation and scanning electron microscopy. Terminal dUTP nick end labeling (TUNEL) and immunofluorescence staining were conducted. Changes in the SIRT1/PGC-1α signaling pathway and other apoptosis-related markers in the cochleae, as well as oxidative stress parameters, were used as readouts. Loss of outer hair cells, more disordered cilia, prominent apoptosis, and elevated free radical levels were observed in the experimental groups. GSRd treatment markedly mitigated hearing threshold shifts, ameliorated outer hair cell loss and lodging or loss of cilia, and improved apoptosis through decreasing Bcl-2 associated X protein (Bax) expression and increasing Bcl-2 expression. In addition, GSRd alleviated the noise-induced cochlear redox injury by upregulating superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels, decreasing malondialdehyde (MDA) levels, and enhancing the activity of SIRT1 and PGC-1α messenger ribonucleic acid (mRNA) and protein expression. In conclusion, GSRd can improve structural and oxidative damage to the cochleae caused by noise. The underlying mechanisms may be associated with the SIRT1/PGC-1α signaling pathway.
    Keywords Panax ; RNA ; apoptosis ; aviation ; electron microscopy ; fluorescent antibody technique ; free radicals ; ginsenosides ; glutathione peroxidase ; glycerol ; inner ear ; males ; malondialdehyde ; noise pollution ; oxidative stress ; pathogenesis ; protective effect ; protein synthesis ; superoxide dismutase
    Language English
    Dates of publication 2023-02
    Size p. 23965-23981.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-022-23504-9
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Protective effect of ginsenoside Rd on military aviation noise-induced cochlear hair cell damage in guinea pigs.

    Chen, Xue-Min / Liu, Yu-Hui / Ji, Shuai-Fei / Xue, Xin-Miao / Wang, Lin-Lin / Zhang, Min / Chang, Yao-Ming / Wang, Xiao-Cheng

    Environmental science and pollution research international

    2022  Volume 30, Issue 9, Page(s) 23965–23981

    Abstract: Noise pollution has become one of the important social hazards that endanger the auditory system of residents, causing noise-induced hearing loss (NIHL). Oxidative stress has a significant role in the pathogenesis of NIHL, in which the silent information ...

    Abstract Noise pollution has become one of the important social hazards that endanger the auditory system of residents, causing noise-induced hearing loss (NIHL). Oxidative stress has a significant role in the pathogenesis of NIHL, in which the silent information regulator 1(SIRT1)/proliferator-activated receptor-gamma coactivator 1α (PGC-1α) signaling pathway is closely engaged. Ginsenoside Rd (GSRd), a main monomer extract from ginseng plants, has been confirmed to suppress oxidative stress. Therefore, the hypothesis that GSRd may attenuate noise-induced cochlear hair cell loss seemed promising. Forty-eight male guinea pigs were randomly divided into four groups: control, noise exposure, GSRd treatment (30 mg/kg Rd for 10d + noise), and experimental control (30 mg/kg glycerol + noise). The experimental groups received military helicopter noise exposure at 115 dB (A) for 4 h daily for five consecutive days. Hair cell damage was evaluated by using inner ear basilar membrane preparation and scanning electron microscopy. Terminal dUTP nick end labeling (TUNEL) and immunofluorescence staining were conducted. Changes in the SIRT1/PGC-1α signaling pathway and other apoptosis-related markers in the cochleae, as well as oxidative stress parameters, were used as readouts. Loss of outer hair cells, more disordered cilia, prominent apoptosis, and elevated free radical levels were observed in the experimental groups. GSRd treatment markedly mitigated hearing threshold shifts, ameliorated outer hair cell loss and lodging or loss of cilia, and improved apoptosis through decreasing Bcl-2 associated X protein (Bax) expression and increasing Bcl-2 expression. In addition, GSRd alleviated the noise-induced cochlear redox injury by upregulating superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels, decreasing malondialdehyde (MDA) levels, and enhancing the activity of SIRT1 and PGC-1α messenger ribonucleic acid (mRNA) and protein expression. In conclusion, GSRd can improve structural and oxidative damage to the cochleae caused by noise. The underlying mechanisms may be associated with the SIRT1/PGC-1α signaling pathway.
    MeSH term(s) Animals ; Guinea Pigs ; Aviation ; Hair Cells, Auditory/metabolism ; Hair Cells, Auditory/pathology ; Hearing Loss, Noise-Induced ; Noise ; Oxidative Stress ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Sirtuin 1/metabolism
    Chemical Substances ginsenoside Rd (WB232T95AV) ; Proto-Oncogene Proteins c-bcl-2 ; Sirtuin 1 (EC 3.5.1.-)
    Language English
    Publishing date 2022-11-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-022-23504-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

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  5. Article ; Online: Small molecules facilitate single factor-mediated sweat gland cell reprogramming.

    Ji, Shuai-Fei / Zhou, Lai-Xian / Sun, Zhi-Feng / Xiang, Jiang-Bing / Cui, Shao-Yuan / Li, Yan / Chen, Hua-Ting / Liu, Yi-Qiong / Gao, Huan-Huan / Fu, Xiao-Bing / Sun, Xiao-Yan

    Military Medical Research

    2022  Volume 9, Issue 1, Page(s) 13

    Abstract: Background: Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands (SG), thus impairing the skin's physiological function. This study aims to develop a stepwise reprogramming strategy to convert ... ...

    Abstract Background: Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands (SG), thus impairing the skin's physiological function. This study aims to develop a stepwise reprogramming strategy to convert fibroblasts into SG lineages, which may provide a promising method to obtain desirable cell types for the functional repair and regeneration of damaged skin.
    Methods: The expression of the SG markers cytokeratin 5 (CK5), cytokeratin 10 (CK10), cytokeratin 18 (CK18), carcino-embryonic antigen (CEA), aquaporin 5 (AQP5) and α-smooth muscle actin (α-SMA) was assessed with quantitative PCR (qPCR), immunofluorescence and flow cytometry. Calcium activity analysis was conducted to test the function of induced SG-like cells (iSGCs). Mouse xenograft models were also used to evaluate the in vivo regeneration of iSGCs. BALB/c nude mice were randomly divided into a normal group, SGM treatment group and iSGC transplantation group. Immunocytochemical analyses and starch-iodine sweat tests were used to confirm the in vivo regeneration of iSGCs.
    Results: EDA overexpression drove HDF conversion into iSGCs in SG culture medium (SGM). qPCR indicated significantly increased mRNA levels of the SG markers CK5, CK18 and CEA in iSGCs, and flow cytometry data demonstrated (4.18 ± 0.04)% of iSGCs were CK5 positive and (4.36 ± 0.25)% of iSGCs were CK18 positive. The addition of chemical cocktails greatly accelerated the SG fate program. qPCR results revealed significantly increased mRNA expression of CK5, CK18 and CEA in iSGCs, as well as activation of the duct marker CK10 and luminal functional marker AQP5. Flow cytometry indicated, after the treatment of chemical cocktails, (23.05 ± 2.49)% of iSGCs expressed CK5
    Conclusion: We developed a SG reprogramming strategy to generate functional iSGCs from HDFs by using the single factor EDA in combination with SGM and small molecules. The generation of iSGCs has important implications for future in situ skin regeneration with SG restoration.
    MeSH term(s) Animals ; Cellular Reprogramming ; Fibroblasts ; Humans ; Mice ; Mice, Nude ; Regeneration ; Sweat Glands/metabolism
    Language English
    Publishing date 2022-03-29
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2768940-2
    ISSN 2054-9369 ; 2054-9369
    ISSN (online) 2054-9369
    ISSN 2054-9369
    DOI 10.1186/s40779-022-00372-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: The relationship between mean platelet volume and diabetic retinopathy: a systematic review and meta-analysis.

    Ji, ShuaiFei / Zhang, Jie / Fan, XiuDe / Wang, XiQiang / Ning, XiaoNa / Zhang, BaBo / Shi, Heng / Yan, Hong

    Diabetology & metabolic syndrome

    2019  Volume 11, Page(s) 25

    Abstract: Background: Diabetic retinopathy (DR) is one of the most common diseases causing blindness in the world, and most patients are already in advanced stage. Recent years, many studies reported mean platelet volume (MPV) may be associated with development ... ...

    Abstract Background: Diabetic retinopathy (DR) is one of the most common diseases causing blindness in the world, and most patients are already in advanced stage. Recent years, many studies reported mean platelet volume (MPV) may be associated with development of DR, but there was no consistent conclusion reached.
    Methods: Literature was retrieved by formally searching PubMed, Embase, Cochrane library and Scopus and by hand searching of reference lists of related articles. Finally, a total of 14 literatures included, and Review manager 5.3 and STATA 14.0 statistical software were utilized for processing.
    Results: Meta-analysis showed that MPV values in DR were significantly higher than health controls [SMD (95% CI) = 0.92 (0.60-1.24)] and type 2 diabetes mellitus without diabetic retinopathy (T2DM without DR) [SMD (95% CI) = 0.36 (0.19-0.53)]. Subgroup analysis indicated that MPV level in proliferative diabetic retinopathy (PDR) patients was higher than T2DM without DR patients [SMD (95% CI) = 0.48 (0.28, 0.68)], but this difference didn't appear in non-proliferative diabetic retinopathy (NPDR).
    Conclusions: The study demonstrated that increased MPV level was significant associated with the development of DR, and it might reflect the severity of DR, which could be provided to monitor development and progression of DR clinically.
    Language English
    Publishing date 2019-03-12
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2518786-7
    ISSN 1758-5996
    ISSN 1758-5996
    DOI 10.1186/s13098-019-0420-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Ginsenoside Rd Ameliorates Auditory Cortex Injury Associated With Military Aviation Noise-Induced Hearing Loss by Activating SIRT1/PGC-1α Signaling Pathway.

    Chen, Xue-Min / Ji, Shuai-Fei / Liu, Yu-Hui / Xue, Xin-Miao / Xu, Jin / Gu, Zheng-Hui / Deng, Sen-Lin / Liu, Cheng-Dong / Wang, Han / Chang, Yao-Ming / Wang, Xiao-Cheng

    Frontiers in physiology

    2020  Volume 11, Page(s) 788

    Abstract: Free radicals and oxidative stress play an important role in the pathogenesis of noise-induced hearing loss (NIHL). Some ginseng monomers showed certain therapeutic effects in NIHL by scavenging free radicals. Therefore, we hypothesized that ginsenoside ... ...

    Abstract Free radicals and oxidative stress play an important role in the pathogenesis of noise-induced hearing loss (NIHL). Some ginseng monomers showed certain therapeutic effects in NIHL by scavenging free radicals. Therefore, we hypothesized that ginsenoside Rd (GSRd) may exert neuroprotective effects after noise-induced auditory system damage through a mechanism involving the SIRT1/PGC-1α signaling pathway. Forty-eight guinea pigs were randomly divided into four equal groups (normal control group, noise group, experimental group that received GSRd dissolved in glycerin through an intraperitoneal injection at a dose of 30 mg/kg body weight from 5 days before noise exposure until the end of the noise exposure period, and experimental control group). Hearing levels were examined by auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE). Hematoxylin-eosin and Nissl staining were used to examine neuron morphology. RT-qPCR and western blotting analysis were used to examine SIRT1/PGC-1α signaling and apoptosis-related genes, including Bax and Bcl-2, in the auditory cortex. Bax and Bcl-2 expression was assessed via immunohistochemistry analysis. Superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) levels were determined using a commercial testing kit. Noise exposure was found to up-regulate ABR threshold and down-regulate DPOAE amplitudes, with prominent morphologic changes and apoptosis of the auditory cortex neurons (
    Language English
    Publishing date 2020-07-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2020.00788
    Database MEDical Literature Analysis and Retrieval System OnLINE

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