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  1. AU="Jia-Wang Wang"
  2. AU="Yin, Yatao"
  3. AU="Daniel Amirault"
  4. AU="Lipson, Laurette"
  5. AU=Wang Yuyi
  6. AU="Toledano-Fonseca, Marta"
  7. AU="Nazarenko, Tatiana A"
  8. AU=Boff Daiane
  9. AU="Michaels, Zachary"
  10. AU="Sovel, Mindy"
  11. AU="Lukyanov, Sergey"
  12. AU="Baptistella, Amanda"
  13. AU="Dichter, Gabriel S"
  14. AU="D Urbano, Vanessa"
  15. AU="Farhad Shirini"
  16. AU="Wu, Wenming"
  17. AU="Wiedermann, Christian J"
  18. AU="Corradin, Giampietro"
  19. AU="Guan, Xiaodong"
  20. AU=Burmester Gerd R.
  21. AU="Mańczak, Rafał"
  22. AU="Cristina Ceron"
  23. AU=Scardapane Arnaldo
  24. AU="Taylor, Daniel J"
  25. AU="Sabanadzovic, Sead"
  26. AU=Lee Yangsoon AU=Lee Yangsoon
  27. AU="Sahoo, Aditi"
  28. AU="Reyes, Peter Andrew C"
  29. AU="Collobert, Géromine"
  30. AU="Guevara, Katterine"
  31. AU=Ahmadivand Arash

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  1. Artikel ; Online: Catalytic asymmetric reductive hydroalkylation of enamides and enecarbamates to chiral aliphatic amines

    Jia-Wang Wang / Yan Li / Wan Nie / Zhe Chang / Zi-An Yu / Yi-Fan Zhao / Xi Lu / Yao Fu

    Nature Communications, Vol 12, Iss 1, Pp 1-

    2021  Band 10

    Abstract: Enantiopure aliphatic amines are frequently encountered as chiral auxiliaries and synthetic intermediates for bioactive compounds. Here, the authors report a mild nickel-catalysed asymmetric reductive hydroalkylation to convert enamides and enecarbamates ...

    Abstract Enantiopure aliphatic amines are frequently encountered as chiral auxiliaries and synthetic intermediates for bioactive compounds. Here, the authors report a mild nickel-catalysed asymmetric reductive hydroalkylation to convert enamides and enecarbamates into α-branched chiral amines and derivatives.
    Schlagwörter Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2021-02-01T00:00:00Z
    Verlag Nature Portfolio
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes

    Bangmei Wang / Kunyu Li / Amy Wang / Michelle Reiser / Thom Saunders / Richard F Lockey / Jia-Wang Wang

    BioTechniques, Vol 59, Iss 4, Pp 201-

    2015  Band 208

    Abstract: The clustered regularly interspaced short palindromic repeat (CRISPR) gene editing technique, based on the non-homologous end-joining (NHEJ) repair pathway, has been used to generate gene knock-outs with variable sizes of small insertion/deletions with ... ...

    Abstract The clustered regularly interspaced short palindromic repeat (CRISPR) gene editing technique, based on the non-homologous end-joining (NHEJ) repair pathway, has been used to generate gene knock-outs with variable sizes of small insertion/deletions with high efficiency. More precise genome editing, either the insertion or deletion of a desired fragment, can be done by combining the homology-directed-repair (HDR) pathway with CRISPR cleavage. However, HDR-mediated gene knock-in experiments are typically inefficient, and there have been no reports of successful gene knock-in with DNA fragments larger than 4 kb. Here, we describe the targeted insertion of large DNA fragments (7.4 and 5.8 kb) into the genomes of mouse embryonic stem (ES) cells and zygotes, respectively, using the CRISPR/HDR technique without NHEJ inhibitors. Our data show that CRISPR/HDR without NHEJ inhibitors can result in highly efficient gene knock-in, equivalent to CRISPR/HDR with NHEJ inhibitors. Although NHEJ is the dominant repair pathway associated with CRISPR-mediated double-strand breaks (DSBs), and biallelic gene knock-ins are common, NHEJ and biallelic gene knock-ins were not detected. Our results demonstrate that efficient targeted insertion of large DNA fragments without NHEJ inhibitors is possible, a result that should stimulate interest in understanding the mechanisms of high efficiency CRISPR targeting in general.
    Schlagwörter CRISPR cleavage ; double-strand break (DSB) ; homology-directed-repair (HDR) ; embryonic stem (ES) cells ; zygote ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 572
    Sprache Englisch
    Erscheinungsdatum 2015-10-01T00:00:00Z
    Verlag Future Science Ltd
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: Inhibition of Apoptosis by the BEACH Domain and WD Repeats of Gene lba that has Key Features of Both Protein Kinase A Anchor and chs1/beige Genes

    Jia-Wang Wang / Julie Howson / Tomar Ghansah / John Ninos / William G. Kerr

    The Scientific World Journal, Vol 1, Pp 96-

    2001  Band 96

    Schlagwörter Science (General) ; Q1-390
    Sprache Englisch
    Erscheinungsdatum 2001-01-01T00:00:00Z
    Verlag Hindawi Publishing Corporation
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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