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  1. Article ; Online: Recent Progress in Porphyrin/g-C 3 N 4 Composite Photocatalysts for Solar Energy Utilization and Conversion

    Sudi Chen / Jiajia Wei / Xitong Ren / Keke Song / Jiajie Sun / Feng Bai / Shufang Tian

    Molecules, Vol 28, Iss 4283, p

    2023  Volume 4283

    Abstract: Transforming solar energy into chemical bonds is a promising and viable way to store solar energy. Porphyrins are natural light-capturing antennas, and graphitic carbon nitride (g-C 3 N 4 ) is an effective, artificially synthesized organic semiconductor. ...

    Abstract Transforming solar energy into chemical bonds is a promising and viable way to store solar energy. Porphyrins are natural light-capturing antennas, and graphitic carbon nitride (g-C 3 N 4 ) is an effective, artificially synthesized organic semiconductor. Their excellent complementarity has led to a growing number of research papers on porphyrin/g-C 3 N 4 hybrids for solar energy utilization. This review highlights the recent progress in porphyrin/g-C 3 N 4 composites, including: (1) porphyrin molecules/g-C 3 N 4 composite photocatalysts connected via noncovalent or covalent interactions, and (2) porphyrin-based nanomaterials/g-C 3 N 4 composite photocatalysts, such as porphyrin-based MOF/g-C 3 N 4 , porphyrin-based COF/g-C 3 N 4 , and porphyrin-based assembly/g-C 3 N 4 heterojunction nanostructures. Additionally, the review discusses the versatile applications of these composites, including artificial photosynthesis for hydrogen evolution, CO 2 reduction, and pollutant degradation. Lastly, critical summaries and perspectives on the challenges and future directions in this field are also provided.
    Keywords porphyrin ; g-C 3 N 4 ; solar energy ; hydrogen generation ; CO 2 reduction ; pollutants degradation ; Organic chemistry ; QD241-441
    Subject code 290
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Synergism of fermented feed and ginseng polysaccharide on growth performance, intestinal development, and immunity of Xuefeng black-bone chickens

    Jie Liu / Huan Wang / Junyi Luo / Ting Chen / Qianyun Xi / Jiajie Sun / Limin Wei / Yongliang Zhang

    BMC Veterinary Research, Vol 20, Iss 1, Pp 1-

    2024  Volume 9

    Abstract: Abstract Microbial fermented feed (MF) is considered a valuable strategy to bring advantages to livestock and is widely practiced. Oral supplementation of Ginseng polysaccharide (Gps) eliminated weight loss in chickens following vaccination. This study ... ...

    Abstract Abstract Microbial fermented feed (MF) is considered a valuable strategy to bring advantages to livestock and is widely practiced. Oral supplementation of Ginseng polysaccharide (Gps) eliminated weight loss in chickens following vaccination. This study investigated the effects of the combined use of Gps and MF on growth performance and immune indices in Xuefeng black-bone chickens. A total of 400 Xuefeng black-bone chickens at the age of 1 day were randomly assigned to four groups. Normal feed group (Control group), ginseng polysaccharide (200 mg/kg) group (Gps group), microbially fermented feed (completely replace the normal feed) group (MF group), and microbially fermented feed and add ginseng polysaccharide just before use (MF + Gps group). Each group contained 5 pens per treatment and 20 birds per pen. The body weight and average daily gain in the Gps, MF, and MF + Gps groups increased significantly (P < 0.01), while the feed conversion ratio decreased significantly (P < 0.01). The combined use of MF and Gps showed a synergistic effect. There was no significant difference in villus height (cecal) between the experimental group and the Con group. The crypt depth of the three experimental groups exhibited a significantly lower value compared to the Control group (P < 0.05). The V/C ratio of the Gps group and MF + Gps was significantly increased (P < 0.05), but there was no significant difference in the MF group. Moreover, the diarrhea rate of the Gps and the MF + Gps groups was lower than that of the Con group, while that of the MF + Gps group decreased the mortality rate (P < 0.05). The serum tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) levels in the MF, Gps, and MF + Gps groups decreased significantly (P < 0.01), the serum immunoglobulin G (IgG) levels increased significantly (P < 0.01), while the combination of MF and Gps had a synergistic effect. The combined use of Gps and MF not only further improved growth performance and immune parameters, but also reduced the ...
    Keywords Ginseng polysaccharide ; Growth performance ; Immune parameters ; Microbial fermented feed ; Synergistic effect ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Exploration of the Effect on Genome-Wide DNA Methylation by miR-143 Knock-Out in Mice Liver

    Xingping Chen / Junyi Luo / Jie Liu / Ting Chen / Jiajie Sun / Yongliang Zhang / Qianyun Xi

    International Journal of Molecular Sciences, Vol 22, Iss 13075, p

    2021  Volume 13075

    Abstract: MiR-143 play an important role in hepatocellular carcinoma and liver fibrosis via inhibiting hepatoma cell proliferation. DNA methyltransferase 3 alpha ( DNMT3a ), as a target of miR-143 , regulates the development of primary organic solid tumors through ...

    Abstract MiR-143 play an important role in hepatocellular carcinoma and liver fibrosis via inhibiting hepatoma cell proliferation. DNA methyltransferase 3 alpha ( DNMT3a ), as a target of miR-143 , regulates the development of primary organic solid tumors through DNA methylation mechanisms. However, the effect of miR-143 on DNA methylation profiles in liver is unclear. In this study, we used Whole-Genome Bisulfite Sequencing (WGBS) to detect the differentially methylated regions (DMRs), and investigated DMR-related genes and their enriched pathways by miR-143 . We found that methylated cytosines increased 0.19% in the miR-143 knock-out (KO) liver fed with high-fat diet (HFD), compared with the wild type (WT). Furthermore, compared with the WT group, the CG methylation patterns of the KO group showed lower CG methylation levels in CG islands (CGIs), promoters and hypermethylation in CGI shores, 5′UTRs, exons, introns, 3′UTRs, and repeat regions. A total of 984 DMRs were identified between the WT and KO groups consisting of 559 hypermethylation and 425 hypomethylation DMRs. Furthermore, DMR-related genes were enriched in metabolism pathways such as carbon metabolism ( serine hydroxymethyltransferase 2 ( Shmt2 ) , acyl-Coenzyme A dehydrogenase medium chain ( Acadm) ), arginine and proline metabolism ( spermine synthase ( Sms ), proline dehydrogenase ( Prodh2 )) and purine metabolism ( phosphoribosyl pyrophosphate synthetase 2 ( Prps2) ). In summary, we are the first to report the change in whole-genome methylation levels by miR-143 -null through WGBS in mice liver, and provide an experimental basis for clinical diagnosis and treatment in liver diseases, indicating that miR-143 may be a potential therapeutic target and biomarker for liver damage-associated diseases and hepatocellular carcinoma.
    Keywords DNA methylation ; miR-143 ; Whole-Genome Bisulfite Sequencing ; liver diseases ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 570
    Language English
    Publishing date 2021-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Photocatalytic Degradation of Tetracycline under Visible Light Irradiation on BiVO 4 Microballs Modified with Noble Metals

    Limeng Wu / Xin Yue / Ying Chang / Kunlei Wang / Jinyue Zhang / Jiajie Sun / Zhishun Wei / Ewa Kowalska

    Catalysts, Vol 12, Iss 1293, p

    2022  Volume 1293

    Abstract: Monoclinic scheelite bismuth vanadate (BVO) microballs were prepared by a facile hydrothermal method and subsequently modified with 2 wt% of noble metals (NM = Au, Ag, Cu, Pt and Pd) by a photodeposition route. All materials were characterized by diffuse ...

    Abstract Monoclinic scheelite bismuth vanadate (BVO) microballs were prepared by a facile hydrothermal method and subsequently modified with 2 wt% of noble metals (NM = Au, Ag, Cu, Pt and Pd) by a photodeposition route. All materials were characterized by diffuse reflectance spectroscopy (DRS), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FTIR). The photocatalytic performance was investigated by degradation of tetracycline antibiotic under visible light irradiation. Moreover, photocurrent generation under UV/vis was also examined. It was found that BVO modification with all tested NMs resulted in a significant improvement in photocatalytic performance. The highest activity was obtained for Cu/BVO with mainly oxidized forms of copper. Based on scavenger tests (∙O 2 − and ∙OH as the main responsible species for TC degradation) and redox properties, it was proposed that the Z-scheme mechanism between copper oxides and BVO was responsible for enhanced photocatalytic activity. However, the co-participation of zero-valent forms of NMs should also be considered, either as electron scavengers, plasmonic sensitizers or conductors. Presented data reveal that porous microballs, highly attractive for practical applications due to micro-sized diameter and efficient light harvesting inside the structure, could be efficiently used for environmental and energy purposes under solar radiation.
    Keywords photocatalysis ; BiVO 4 ; degradation of pollutants ; noble metals ; photocurrent ; surface modification ; Chemical technology ; TP1-1185 ; Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2022-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation

    Jiali Xiong / Haojie Zhang / Yuxuan Wang / Yunyun Cheng / Junyi Luo / Ting Chen / Qianyun Xi / Jiajie Sun / Yongliang Zhang

    International Journal of Molecular Sciences, Vol 23, Iss 1413, p

    2022  Volume 1413

    Abstract: 1) Background: As a novel type of non-coding RNA with a stable closed-loop structure, circular RNA (circRNA) can interact with microRNA (miRNA) and influence the expression of miRNA target genes. However, circRNA involved in pituitary growth hormone (GH) ...

    Abstract (1) Background: As a novel type of non-coding RNA with a stable closed-loop structure, circular RNA (circRNA) can interact with microRNA (miRNA) and influence the expression of miRNA target genes. However, circRNA involved in pituitary growth hormone (GH) regulation is poorly understood. Our previous study revealed protein kinase C alpha ( PRKCA ) as the target gene of miR-709. Currently, the expression and function of rno_circRNA_0001004 in the rat pituitary gland is not clarified; (2) Methods: In this study, both bioinformatics analysis and dual-luciferase report assays showed a target relationship between rno_circRNA_0001004 and miR-709. Furthermore, the rno_circRNA_0001004 overexpression vector and si-circ_0001004 were constructed and transfected into GH 3 cells; (3) Results: We found that rno_circRNA_0001004 expression was positively correlated with the PRKCA gene and GH expression levels, while it was negatively correlated with miR-709. In addition, overexpression of rno-circ_0001004 also promoted proliferation and relieved the inhibition of miR-709 in GH 3 cells; (4) Conclusions: Our findings show that rno_circ_0001004 acts as a novel sponge for miR-709 to regulate GH synthesis and cell proliferation, and are the first case of discovery of the regulatory role of circRNA_0001004 in pituitary GH.
    Keywords pituitary ; rno_circ_0001004 ; miR-709 ; GH ; proliferation ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 500
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: CircEZH2 Regulates Milk Fat Metabolism through miR-378b Sponge Activity

    Dongyang Wang / Zhengjiang Zhao / Yiru Shi / Junyi Luo / Ting Chen / Qianyun Xi / Yongliang Zhang / Jiajie Sun

    Animals, Vol 12, Iss 718, p

    2022  Volume 718

    Abstract: In this study, we evaluated the roles of heat-induced circEZH2 in the regulation of milk fat metabolism. CircEZH2 overexpression increased HC11 cell proliferation and decreased apoptosis. These changes were accompanied by increased expression of ... ...

    Abstract In this study, we evaluated the roles of heat-induced circEZH2 in the regulation of milk fat metabolism. CircEZH2 overexpression increased HC11 cell proliferation and decreased apoptosis. These changes were accompanied by increased expression of proliferation marker proteins (PCNA, Cyclin D, and Cyclin E) and the anti-apoptotic protein Bcl2, while expression of the pro-apoptotic proteins Bax and cleaved-caspase was reduced. SiRNA-mediated silencing of EZH2 in HC11 cells had the opposite effects. CircEZH2 overexpression promoted the uptake of a fluorescent fatty acid (Bodipy) as well as expression of the fatty acid transport-related protein CD36, lipolysis-related protein LPL, and unsaturated fatty acid metabolism-related proteins FADS1 and SCD1. Dual luciferase reporter assays verified the targeting relationship of the two ceRNA networks, circEZH2-miR378b-LPL and circEZH2-miR378b-CD36. This information provides further clarification of the role of circRNAs in milk fat regulation in addition to a theoretical basis for alleviating the effects of heat stress on milk production by dairy cows.
    Keywords circEZH2 ; proliferation ; apoptosis ; miR378b ; fatty acid metabolism ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 570
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Milk exosome-derived miRNAs from water buffalo are implicated in immune response and metabolism process

    Zujing Chen / Yueqin Xie / Junyi Luo / Ting Chen / Qianyun Xi / Yongliang Zhang / Jiajie Sun

    BMC Veterinary Research, Vol 16, Iss 1, Pp 1-

    2020  Volume 5

    Abstract: Abstract Background Buffalo milk is rich in various nutritional components and bioactive substances that provide more essential health benefits to human body. Recently, exosome identified in the breast milk has been reported as a neotype nutrient and can ...

    Abstract Abstract Background Buffalo milk is rich in various nutritional components and bioactive substances that provide more essential health benefits to human body. Recently, exosome identified in the breast milk has been reported as a neotype nutrient and can mediate intercellular communication with exosomal miRNAs. In the present study, we therefore hypothesized that exosome-derived miRNAs from buffalo milk would play the potential physiological importance of consumption of buffalo milk. Results We isolated exosomes from buffalo and cow milk samples that were obtained at mid-lactation period, and the exosomal miRNA profiles were then generated using miRNA-seq. In addition, miRNAomes of pig, human and panda milk exosomes were downloaded from GEO database. Finally, a total of 27 milk exosomal miRNA profiles that included 4 buffalo, 4 cow, 8 pig, 4 human and 7 panda were analyzed using the miRDeep2 program. A total of 558 unique miRNA candidates existed across all species, and the top 10 highly expressed miRNA were evolutionarily conserved across multiple species. Functional analysis revealed that these milk enriched miRNAs targeted 400 putative sites to modulate disease resistance, immune responsiveness and basic metabolism events. In addition, a total of 32 miRNAs in buffalo milk were significantly up-regulated compared with non-buffalo milks, while 16 were significantly down-regulated. Of interest, functional analysis showed that up-regulated miRNAs were mainly related to host metabolism processes, while the predicted functions of down-regulated miRNAs were enriched in immune response. Conclusion In this study, we explored the exosomal miRNAome differences between milks of different animals, expanding the theoretical basis for potential applications of the miRNA-containing vesicles.
    Keywords Buffalo milk ; Exosomal miRNA ; Cell-cell communication ; Immune and metabolism ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2020-04-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Fermentation quality of herbal tea residue and its application in fattening cattle under heat stress

    Xiaona Zhuang / Zujing Chen / Xiaohong Sun / Fangjun Li / Junyi Luo / Ting Chen / Qianyun Xi / Yongliang Zhang / Jiajie Sun

    BMC Veterinary Research, Vol 17, Iss 1, Pp 1-

    2021  Volume 13

    Abstract: Abstract Background Herbal tea residue (HTR) is generally considered to be the waste of herbal tea beverage production while it still retains rich nutrients and active substances. The main aim of the present study was to investigate the effect of ... ...

    Abstract Abstract Background Herbal tea residue (HTR) is generally considered to be the waste of herbal tea beverage production while it still retains rich nutrients and active substances. The main aim of the present study was to investigate the effect of fermentation technology on improving the quality of HTRs, and focus on the fermented HTR-induced alleviation of summer heat stress in fattening cattle. Results In this study, the waste HTR was fermented and then fed to a total of 45 fattening cattle that were divided into 3 groups (fermented HTR replaced 0, 15, 30% of the forage component of the diet), and the feeding experiment was lasted for 40 days. The physiological indexes, growth performance and fecal microbiota of fattening cattle were evaluated and results showed that fermented HTR could effectively reduce the respiratory rate and rectal temperature of fattening cattle under heat stress, increase the daily feed intake and daily gain, and improve the antioxidant content and blood immune index. In addition, we studied the fecal microbiota composition of 6 fattening cattle in control and 30% HTR substitution groups and found fermented HTR significantly changed the composition of fecal microbiota and increased microbial diversity, and correlation analysis suggested that the bacteria were closely related to fecal SCFA levels of fattening cattle under heat stress. Conclusions In this study, fermented HTR replaced 30% of the forage component of the diet that can change the intestine microorganisms, maintain health and alleviate the heat stress of fattening cattle.
    Keywords Herbal tea residue ; Microorganism ; Fattening cattle ; Fermented feed ; Heat stress ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2021-11-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Identification of circRNA-Associated-ceRNA Networks Involved in Milk Fat Metabolism under Heat Stress

    Dongyang Wang / Zujing Chen / Xiaona Zhuang / Junyi Luo / Ting Chen / Qianyun Xi / Yongliang Zhang / Jiajie Sun

    International Journal of Molecular Sciences, Vol 21, Iss 4162, p

    2020  Volume 4162

    Abstract: Summer temperatures are generally high in Southern China, and cows are likely to suffer a heat stress reaction. Heat stress will have a negative impact on the performance of dairy cows; however, the mechanism by which high temperature affects lactation ... ...

    Abstract Summer temperatures are generally high in Southern China, and cows are likely to suffer a heat stress reaction. Heat stress will have a negative impact on the performance of dairy cows; however, the mechanism by which high temperature affects lactation is not clear. CircRNA is a type of non-coding RNA discovered in recent years, which performs a crucial function in many biological activities. However, the effects of circRNA on lactation function of dairy cows under heat stress is unknown. The present study aimed to explore the expression levels of circRNA in the mammary gland tissue of cows under heat stress. Firstly, we collected blood and milk samples of summer and winter cows and evaluated lactation performance using serum indicators, milk production, and milk composition. Incorporating the calculation of the temperature and humidity index, we conformed the heat stress status of cows in summer. Heat stress increased the concentration of HSP70 and decreased the concentration of SOD and PRL. Heat stress not only reduced milk yield but also affected milk quality, with milk lactose and milk protein decreasing with increased temperature. The analysis of the fatty acid composition in summer milk found significantly reduced concentrations of unsaturated fatty acids, especially long-chain unsaturated fatty acids. Sequencing of the cow’s mammary gland transcriptome revealed that compared to the appropriate temperature (ST) group, the heat stress (HS) group had a total of 2204 upregulated and 3501 downregulated transcripts. GO enrichment and KEGG pathway analysis showed that these genes were mainly related to milk fat metabolism. In addition, 19 upregulated and 19 downregulated circRNA candidates were found in response to heat stress. We used Pearson’s test to establish the correlation of circRNA-mRNA and identified four pairs of circRNA-miRNA networks between four circRNAs, six miRNAs, and the CD36 gene. In this study, we revealed the possible role of circRNAs in lactation of dairy cows and identified that ...
    Keywords heat stress ; milk fat ; ceRNA ; CD36 ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 630
    Language English
    Publishing date 2020-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.

    Yueqin Xie / Jie Liu / Huan Wang / Junyi Luo / Ting Chen / Qianyun Xi / Yongliang Zhang / Jiajie Sun

    PLoS ONE, Vol 15, Iss 8, p e

    2020  Volume 0237357

    Abstract: Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine. Ginseng polysaccharides exhibit several biological activities and ...

    Abstract Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine. Ginseng polysaccharides exhibit several biological activities and contribute to improving intestinal development. Here, Xuefeng black-bone chickens were fed a basal diet fermented by Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecium, with or without ginseng polysaccharides. The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum. In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected. Next, we studied the intestinal microbial composition of 32 Xuefeng black-bone chickens. A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla. At the genus level, Sutterella and Asteroleplasma abundance increased and decreased, respectively, in the FP and Po groups. Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio. At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance. Moreover, bacterial abundance was closely related to the jejunum histomorphology. Asteroleplasma_anaerobium abundance was positively correlated with crypt depth ...
    Keywords Medicine ; R ; Science ; Q
    Subject code 333
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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