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  1. Article: Analysis of the Genetic Diversity in Tea Plant Germplasm in Fujian Province Based on Restriction Site-Associated DNA Sequencing.

    Jiang, Lele / Xie, Siyi / Zhou, Chengzhe / Tian, Caiyun / Zhu, Chen / You, Xiaomei / Chen, Changsong / Lai, Zhongxiong / Guo, Yuqiong

    Plants (Basel, Switzerland)

    2023  Volume 13, Issue 1

    Abstract: Fujian province, an important tea-producing area in China, has abundant tea cultivars. To investigate the genetic relationships of tea plant cultivars in Fujian province and the characteristics of the tea plant varieties, a total of 70 tea cultivars from ...

    Abstract Fujian province, an important tea-producing area in China, has abundant tea cultivars. To investigate the genetic relationships of tea plant cultivars in Fujian province and the characteristics of the tea plant varieties, a total of 70 tea cultivars from Fujian and other 12 provinces in China were subjected to restriction site-associated DNA sequencing (RAD-seq). A total of 60,258,975 single nucleotide polymorphism (SNP) sites were obtained. These 70 tea plant cultivars were divided into three groups based on analyzing the phylogenetic tree, principal component, and population structure. Selection pressure analysis indicated that nucleotide diversity was high in Southern China and genetically distinct from cultivars of Fujian tea plant cultivars, according to selection pressure analysis. The selected genes have significant enrichment in pathways associated with metabolism, photosynthesis, and respiration. There were ten characteristic volatiles screened by gas chromatography-mass spectrometry (GC-MS) coupled with multivariate statistical methods, among which the differences in the contents of methyl salicylate, 3-carene, cis-3-hexen-1-ol, (
    Language English
    Publishing date 2023-12-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants13010100
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Stimulatory and inhibitory effects of phenanthrene on physiological performance of Chlorella vulgaris and Skeletonema costatum.

    Jiang, Lele / Pan, Yueqiang / Zhu, Shaoting / Qiu, Jingmin / Shang, Yu / Xu, Juntian / Li, Futian / Wang, Hongbin

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 5194

    Abstract: The effects of polycyclic aromatic hydrocarbons on phytoplankton have been extensively documented, but there is limited knowledge about the physiological responses of marine primary producers to phenanthrene at environmentally relevant levels. Here, we ... ...

    Abstract The effects of polycyclic aromatic hydrocarbons on phytoplankton have been extensively documented, but there is limited knowledge about the physiological responses of marine primary producers to phenanthrene at environmentally relevant levels. Here, we investigated the toxicity of phenanthrene (0, 1, and 5 or 10 μg L
    MeSH term(s) Chlorella vulgaris ; Diatoms ; Phenanthrenes/pharmacology ; Photosynthesis ; Phytoplankton
    Chemical Substances Phenanthrenes
    Language English
    Publishing date 2022-03-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-08733-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Cell-free protein synthesis of CD1E and B2M protein and in vitro interaction.

    Tang, Yajie / Ma, Shengming / Lin, Sen / Wu, Yinrong / Chen, Siyang / Liu, Gang / Ma, Lisong / Wang, Zaihua / Jiang, Lele / Wang, Yao

    Protein expression and purification

    2022  Volume 203, Page(s) 106209

    Abstract: CD1E, one of the most important glycolipid antigens on T cell membranes, is required for glycolipid antigen presentation on the cell surface. Cell-based recombinant expression systems have many limitations for synthesizing transmembrane proteins such as ... ...

    Abstract CD1E, one of the most important glycolipid antigens on T cell membranes, is required for glycolipid antigen presentation on the cell surface. Cell-based recombinant expression systems have many limitations for synthesizing transmembrane proteins such as CD1E, including low protein yields and miss folding. To overcome these challenges, here we successfully synthesized high-quality soluble CD1E using an E.coli cell-free protein synthesis system (CFPS) with the aid of detergent. Following purification by Ni
    MeSH term(s) Glycolipids/metabolism ; Membrane Proteins/metabolism ; Cell Membrane/metabolism ; Cell-Free System/metabolism
    Chemical Substances Glycolipids ; Membrane Proteins
    Language English
    Publishing date 2022-11-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1055455-5
    ISSN 1096-0279 ; 1046-5928
    ISSN (online) 1096-0279
    ISSN 1046-5928
    DOI 10.1016/j.pep.2022.106209
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3084: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  4. Article ; Online: Comparative study of the physiological responses of Skeletonema costatum and Thalassiosira weissflogii to initial pCO

    Qiu, Jingmin / Su, Tianci / Wang, Xin / Jiang, Lele / Shang, Yu / Jin, Peng / Xu, Juntian / Fan, Jiale / Li, Wei / Li, Futian

    Marine environmental research

    2022  Volume 175, Page(s) 105581

    Abstract: Extensive studies have documented the responses of diatoms to environmental drivers in the context of climate change. However, bloom dynamics are usually ignored in most studies. Here, we investigated the effects of the initial ... ...

    Abstract Extensive studies have documented the responses of diatoms to environmental drivers in the context of climate change. However, bloom dynamics are usually ignored in most studies. Here, we investigated the effects of the initial pCO
    MeSH term(s) Batch Cell Culture Techniques ; Carbon Dioxide ; Diatoms/physiology ; Hydrogen-Ion Concentration ; Seawater
    Chemical Substances Carbon Dioxide (142M471B3J)
    Language English
    Publishing date 2022-02-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 1502505-6
    ISSN 1879-0291 ; 0141-1136
    ISSN (online) 1879-0291
    ISSN 0141-1136
    DOI 10.1016/j.marenvres.2022.105581
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  5. Article: Combined effects of seawater acidification and benzo(a)pyrene on the physiological performance of the marine bloom-forming diatom Skeletonema costatum

    Li, Futian / Jiang, Lele / Zhang, Tianzhi / Qiu, Jingmin / Lv, Dongmei / Su, Tianci / Li, Wei / Xu, Juntian / Wang, Hongbin

    Marine environmental research. 2021 July, v. 169

    2021  

    Abstract: The combined effects of polycyclic aromatic hydrocarbons and seawater acidification are poorly understood. Hence, we exposed the bloom-forming diatom Skeletonema costatum to four concentrations (0, 0.1, 1 and 10 μg L⁻¹) of benzo(a)pyrene and two pCO₂ ... ...

    Abstract The combined effects of polycyclic aromatic hydrocarbons and seawater acidification are poorly understood. Hence, we exposed the bloom-forming diatom Skeletonema costatum to four concentrations (0, 0.1, 1 and 10 μg L⁻¹) of benzo(a)pyrene and two pCO₂ levels (400 and 1000 μatm) to investigate its physiological performance. The growth and photosynthesis of S. costatum were tolerant to low and moderate benzo(a)pyrene concentrations regardless of the pCO₂ level. However, the highest benzo(a)pyrene concentration had remarkably adverse effects on most parameters, decreasing the growth rate by 69%. Seawater acidification increased the sensitivity to high light stress, as shown by the lower maximum relative electron transport rate and light saturation point at the highest benzo(a)pyrene concentration. Our results suggested that benzo(a)pyrene could be detrimental to diatoms at a habitat-relevant level, and seawater acidification might further decrease its light tolerance, which would have important ramifications for the community structure and primary production in coastal waters.
    Keywords Bacillariophyceae ; Skeletonema costatum ; community structure ; electron transfer ; ocean acidification ; photosynthesis ; primary productivity ; research
    Language English
    Dates of publication 2021-07
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1502505-6
    ISSN 1879-0291 ; 0141-1136
    ISSN (online) 1879-0291
    ISSN 0141-1136
    DOI 10.1016/j.marenvres.2021.105396
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  6. Article: Comparative study of the physiological responses of Skeletonema costatum and Thalassiosira weissflogii to initial pCO2 in batch cultures, with special reference to bloom dynamics

    Qiu, Jingmin / Su, Tianci / Wang, Xin / Jiang, Lele / Shang, Yu / Jin, Peng / Xu, Juntian / Fan, Jiale / Li, Wei / Li, Futian

    Marine environmental research. 2022 Mar., v. 175

    2022  

    Abstract: Extensive studies have documented the responses of diatoms to environmental drivers in the context of climate change. However, bloom dynamics are usually ignored in most studies. Here, we investigated the effects of the initial pCO₂ on the bloom ... ...

    Abstract Extensive studies have documented the responses of diatoms to environmental drivers in the context of climate change. However, bloom dynamics are usually ignored in most studies. Here, we investigated the effects of the initial pCO₂ on the bloom characteristics of two cosmopolitan diatoms, Skeletonema costatum and Thalassiosira weissflogii. Batch cultures with two initial pCO₂ conditions (LC: 400 μatm; HC: 1000 μatm) were used to investigate bloom dynamics under current and ocean acidification scenarios. The simulated S. costatum bloom was characterized by fast accumulation, a rapid decline in biomass, and a shorter stationary phase. The T. weissflogii bloom had a longer stationary phase, and cell density remained at high levels after culturing for 19 days. The physiological performances of the two diatoms varied significantly in the different bloom phases. We found that the initial pCO₂ has modulating effects on biomass accumulation and bloom dynamics for these two diatoms. The higher initial pCO₂ enhanced the specific growth rate of T. weissflogii by 6% in the exponential phase, leading to higher cell densities, while 86% higher decay rates were observed in the HC cultures of S. costatum. Overall, ocean acidification may alter the dynamics of diatom blooms and may have profound impacts on the biological carbon pump.
    Keywords Bacillariophyceae ; Skeletonema costatum ; Thalassiosira ; biomass production ; carbon ; climate change ; comparative study ; ocean acidification ; research ; specific growth rate
    Language English
    Dates of publication 2022-03
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1502505-6
    ISSN 1879-0291 ; 0141-1136
    ISSN (online) 1879-0291
    ISSN 0141-1136
    DOI 10.1016/j.marenvres.2022.105581
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  7. Article ; Online: Multiomics Analysis Reveals the Involvement of JsLHY in Controlling Aroma Production in Jasmine Flowers.

    Zhou, Chengzhe / Tian, Caiyun / Wen, Shengjing / Yang, Niannian / Zhang, Cheng / Zheng, Anru / Tan, Jiayao / Jiang, Lele / Zhu, Chen / Lai, Zhongxiong / Lin, Yuling / Guo, Yuqiong

    Journal of agricultural and food chemistry

    2023  

    Abstract: ... ...

    Abstract The
    Language English
    Publishing date 2023-11-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.3c05768
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Article ; Online: Combined effects of seawater acidification and benzo(a)pyrene on the physiological performance of the marine bloom-forming diatom Skeletonema costatum.

    Li, Futian / Jiang, Lele / Zhang, Tianzhi / Qiu, Jingmin / Lv, Dongmei / Su, Tianci / Li, Wei / Xu, Juntian / Wang, Hongbin

    Marine environmental research

    2021  Volume 169, Page(s) 105396

    Abstract: The combined effects of polycyclic aromatic hydrocarbons and seawater acidification are poorly understood. Hence, we exposed the bloom-forming diatom Skeletonema costatum to four concentrations (0, 0.1, 1 and 10 μg ... ...

    Abstract The combined effects of polycyclic aromatic hydrocarbons and seawater acidification are poorly understood. Hence, we exposed the bloom-forming diatom Skeletonema costatum to four concentrations (0, 0.1, 1 and 10 μg L
    MeSH term(s) Benzo(a)pyrene/toxicity ; Diatoms ; Hydrogen-Ion Concentration ; Photosynthesis ; Seawater
    Chemical Substances Benzo(a)pyrene (3417WMA06D)
    Language English
    Publishing date 2021-06-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 1502505-6
    ISSN 1879-0291 ; 0141-1136
    ISSN (online) 1879-0291
    ISSN 0141-1136
    DOI 10.1016/j.marenvres.2021.105396
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes.

    Alghalayini, Amani / Jiang, Lele / Gu, Xi / Yeoh, Guan Heng / Cranfield, Charles G / Timchenko, Victoria / Cornell, Bruce A / Valenzuela, Stella M

    Journal of visualized experiments : JoVE

    2020  , Issue 166

    Abstract: Here we report a protocol to investigate the heat transfer between irradiated gold nanoparticles (GNPs) and bilayer lipid membranes by electrochemistry using tethered bilayer lipid membranes (tBLMs) assembled on gold electrodes. Irradiated modified GNPs, ...

    Abstract Here we report a protocol to investigate the heat transfer between irradiated gold nanoparticles (GNPs) and bilayer lipid membranes by electrochemistry using tethered bilayer lipid membranes (tBLMs) assembled on gold electrodes. Irradiated modified GNPs, such as streptavidin-conjugated GNPs, are embedded in tBLMs containing target molecules, such as biotin. By using this approach, the heat transfer processes between irradiated GNPs and model bilayer lipid membrane with entities of interest are mediated by a horizontally focused laser beam. The thermal predictive computational model is used to confirm the electrochemically induced conductance changes in the tBLMs. Under the specific conditions used, detecting heat pulses required specific attachment of the gold nanoparticles to the membrane surface, while unbound gold nanoparticles failed to elicit a measurable response. This technique serves as a powerful detection biosensor which can be directly utilized for the design and development of strategies for thermal therapies that permits optimization of the laser parameters, particle size, particle coatings and composition.
    MeSH term(s) Electric Conductivity ; Gold/chemistry ; Hot Temperature ; Lipid Bilayers/chemistry ; Membrane Lipids/chemistry ; Metal Nanoparticles/chemistry
    Chemical Substances Lipid Bilayers ; Membrane Lipids ; Gold (7440-57-5)
    Language English
    Publishing date 2020-12-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/61851
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Real-time monitoring of heat transfer between gold nanoparticles and tethered bilayer lipid membranes.

    Alghalayini, Amani / Jiang, Lele / Gu, Xi / Yeoh, Guan Heng / Cranfield, Charles G / Timchenko, Victoria / Cornell, Bruce A / Valenzuela, Stella M

    Biochimica et biophysica acta. Biomembranes

    2020  Volume 1862, Issue 9, Page(s) 183334

    Abstract: Plasmon resonance frequency irradiated gold nanoparticles (GNPs) have gained interest as a laser-targeted treatment for infections, tumors and for the controlled release of drugs in situ. Questions still remain, however, as to the efficiency of heat ... ...

    Abstract Plasmon resonance frequency irradiated gold nanoparticles (GNPs) have gained interest as a laser-targeted treatment for infections, tumors and for the controlled release of drugs in situ. Questions still remain, however, as to the efficiency of heat delivery within biological tissues and how this can be reliably determined. Here, we demonstrate how a nanomaterial-electrode interface that mimics cell membranes can detect the localized heat transfer characteristics arising from plasmon resonance frequency-matched laser excitation of GNPs. We demonstrate that the lipid bilayer membrane can be affected by conjugated GNP induced hyperthermia when irradiated with a laser power output as low as 135 nW/μm
    MeSH term(s) Cell Membrane/chemistry ; Cell Membrane/metabolism ; Gold/chemistry ; Gramicidin/chemistry ; Gramicidin/metabolism ; Hot Temperature ; Lipid Bilayers/chemistry ; Lipid Bilayers/metabolism ; Metal Nanoparticles/chemistry ; Peptides/chemistry ; Peptides/metabolism ; Proteins
    Chemical Substances Lipid Bilayers ; Peptides ; Proteins ; Gramicidin (1405-97-6) ; Gold (7440-57-5)
    Language English
    Publishing date 2020-05-04
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbamem.2020.183334
    Database MEDical Literature Analysis and Retrieval System OnLINE

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