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Book: Plant protein secretion

Jiang, Liwen

methods and protocols

(Methods in molecular biology ; 1662 ; Springer protocols)

2017

Author's details	edited by Liwen Jiang
Series title	Methods in molecular biology ; 1662 Springer protocols
Collection
Keywords	Trafficking pathways ; Protein secretion ; Bioinformatics ; Biochemical assays ; Organelle proteomics
Subject code	580
Language	English
Size	xi, 285 Seiten, Illustrationen
Publisher	Humana Press
Publishing place	New York, NY
Publishing country	United States
Document type	Book
HBZ-ID	HT019404371
ISBN	978-1-4939-7261-6 ; 1-4939-7261-8 ; 9781493972623 ; 1493972626
Database	Catalogue ZB MED Medicine, Health

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Article: Untargeted metabolomics analysis of Mucor racemosus Douchi fermentation process by gas chromatography with time‐of‐flight mass spectrometry

Li, Pao / Jiang, Liwen

Food Science & Nutrition, 7(5):1865-1874

2019

Abstract: Intensive study of the metabolome during the Douchi fermentation can provide new knowledge for optimizing the fermentation process. In this work, the metabolic characterization throughout the fermentation of Mucor racemosus Douchi was investigated using ... ...

Abstract	Intensive study of the metabolome during the Douchi fermentation can provide new knowledge for optimizing the fermentation process. In this work, the metabolic characterization throughout the fermentation of Mucor racemosus Douchi was investigated using gas chromatography with time‐of‐flight mass spectrometry. A total of 511 peaks were found, and 114 metabolites were identified. The fermentation process was clearly distinguished into two main phases by principal components analysis and orthogonal partial least squares‐discriminant analysis. All the samples in the score plots were within the 95% Hotelling T2 ellipse. Two separated clusters can be seen clearly in the score plot, which represents the two stages of fermentation: koji‐making (within 48 hr) and postfermentation (after 48 hr). Besides, clear separation and discrimination by both methods were found among different fermentation time within 15 days, while the discrimination cannot be found with more than 15 days of fermentation, indicating that the fermentation of Douchi was finished in 15 days. Due to the synergistic effect of protease and hydrolase accumulated in the early stage, proteins and other big molecular substances are rapidly hydrolyzed into a large number of small molecule components. However, the activity of enzymes decreased with the further fermentation, and some free amino acids were consumed in Maillard reaction. Therefore, there was no significant change in the content of small molecular substances after 15 days of fermentation. Furthermore, the levels of some metabolites such as alanine and lysine involved in the fermentation varied significantly throughout the processes. This study provides new insights for the metabolomics characteristics of Douchi fermentation.
Keywords	GC‐TOF/MS ; Douchi fermentation ; orthogonal partial least squares‐discriminant analysis ; metabolic characterization ; principal components analysis
Language	English
Document type	Article
Database	Repository for Life Sciences

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Article ; Online: The seven rice vacuolar sorting receptors localize to prevacuolar compartments.

Yang, Lei / Jiang, Liwen

Journal of plant physiology

2023 Volume 291, Page(s) 154137

Abstract: Vacuolar sorting is critically important in plants as it regulates the mobilization of proteins and plays a major role in important agricultural traits like yield and seed protein content. Vacuolar sorting receptors (VSRs) are integral membrane proteins ... ...

Abstract	Vacuolar sorting is critically important in plants as it regulates the mobilization of proteins and plays a major role in important agricultural traits like yield and seed protein content. Vacuolar sorting receptors (VSRs) are integral membrane proteins that mediate protein trafficking from the Golgi apparatus to the vacuole via the intermediate membrane-bound prevacuolar compartment (PVC)/multivesicular body (MVB). VSR proteins, such as an 80 kD (BP-80) from pea, also serve as markers for PVC/MVB. Dissecting VSR-mediated protein trafficking pathways may provide ways to enhance agronomic traits and crop yield. Green fluorescence protein (GFP) fusions with the seven Arabidopsis (Arabidopsis thaliana) VSRs were previously shown to localize to PVCs in transgenic tobacco BY-2 cells. The Rice (Oryza sativa) genome contains seven VSRs (OsVSR1-7), but little is known about their subcellular localizations. Here we studied the subcellular localization of OsVSR1-7 b y using a reporter approach, in which GFP-OsVSR1-7 fusions containing the transmembrane domain (TMD) and cytoplasmic tail (CT) of individual OsVSR were expressed in the protoplasts of rice, transgenic tobacco BY-2 cells and transgenic rice plants. Immunofluorescent labelling studies and confocal laser scanning microscope observation demonstrated that the seven OsVSRs are localized to PVCs and form ring-like structures upon wortmannin treatment. Therefore, we have verified the subcellular localization of OsVSR1-7 in this study. The OsVSRs tagged with GFP can serve as PVCs/MVBs markers in rice for the future studies.
MeSH term(s)	Vacuoles/metabolism ; Oryza/genetics ; Oryza/metabolism ; Protein Transport ; Wortmannin/metabolism ; Arabidopsis/genetics ; Arabidopsis/metabolism ; Green Fluorescent Proteins/metabolism
Chemical Substances	Wortmannin (XVA4O219QW) ; Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2023-11-10
Publishing country	Germany
Document type	Journal Article
ZDB-ID	283647-6
ISSN	1618-1328 ; 0176-1617
ISSN (online)	1618-1328
ISSN	0176-1617
DOI	10.1016/j.jplph.2023.154137
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Article ; Online: Ufmylation bridges autophagy and ER homeostasis in plants.

Li, Baiying / Jiang, Liwen

Autophagy

2023 Volume 19, Issue 10, Page(s) 2830–2831

Abstract: The autophagic machinery is highly conserved in eukaryotes. Plants, as sessile organisms, are more susceptible to environmental stresses than animals. Autophagy plays a pivotal role in plant stress responses, but the regulation of autophagic flux in ... ...

Abstract	The autophagic machinery is highly conserved in eukaryotes. Plants, as sessile organisms, are more susceptible to environmental stresses than animals. Autophagy plays a pivotal role in plant stress responses, but the regulation of autophagic flux in plants remains enigmatic with few autophagic receptors identified. We recently characterized an E3 ligase, the ubiquitin-fold modifier 1 (Ufm1) ligase 1 (Ufl1), as well as its small modifier protein Ufm1, as interactors of the core autophagy-related (ATG) proteins. Mutants of these ufmylation system components are hypersensitive to salt stress and trigger the upregulation of endoplasmic reticulum (ER) stress-responsive genes, as well as the accumulation of ER sheets caused by a defect in reticulophagy. Increased expression of Ufl1, Ufm1 and Ufm1-conjugating enzyme 1 (Ufc1) are also triggered by salt stress in plants. This study identified and demonstrated the participation of ufmylation components in maintaining ER homeostasis by regulating reticulophagy under salt stress in plants.
MeSH term(s)	Arabidopsis/metabolism ; Autophagy ; Endoplasmic Reticulum/metabolism ; Endoplasmic Reticulum Stress ; Homeostasis ; Plant Proteins/metabolism ; Plants/metabolism ; Salt Stress ; Ubiquitin-Protein Ligases/metabolism
Chemical Substances	Plant Proteins ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
Language	English
Publishing date	2023-05-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2454135-7
ISSN	1554-8635 ; 1554-8627
ISSN (online)	1554-8635
ISSN	1554-8627
DOI	10.1080/15548627.2023.2203985
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Article ; Online: FREE1 regulates phagophore closure in plants.

Zhu, Ying / Zeng, Yonglun / Jiang, Liwen

Autophagy

2024 , Page(s) 1–3

Abstract: Adenosine monophosphate-activated protein kinase (AMPK), the central energy sensor in more complex eukaryotes, can activate macroautophagy/autophagy upon cellular energy deficiency. However, the regulatory role of nutrient sensing in mediating phagophore ...

Abstract	Adenosine monophosphate-activated protein kinase (AMPK), the central energy sensor in more complex eukaryotes, can activate macroautophagy/autophagy upon cellular energy deficiency. However, the regulatory role of nutrient sensing in mediating phagophore closure to generate an autophagosome remains unknown. The evolutionarily conserved endosomal sorting complexes required for transport (ESCRT) machinery has been postulated to regulate phagophore sealing, yet the signaling pathway modulating the ESCRT complex relocation from multivesicular body (MVB) to phagophore for closure remains unknown. We recently identified a plant unique pleiotropic protein FREE1 (FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1), which is phosphorylated by the plant energy sensor SnRK1 (SNF1-related kinase 1) and bridges the ATG conjugation system and ESCRT machinery to regulate phagophore sealing upon nutrient starvation. This study elucidated the bona fide roles and underlying mechanism of cellular energy-sensing pathways in regulating compartment sealing.
Language	English
Publishing date	2024-02-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2454135-7
ISSN	1554-8635 ; 1554-8627
ISSN (online)	1554-8635
ISSN	1554-8627
DOI	10.1080/15548627.2024.2312793
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Article ; Online: Autophagosome biogenesis and organelle homeostasis in plant cells.

Zhuang, Xiaohong / Li, Baiying / Jiang, Liwen

The Plant cell

2024

Abstract: Autophagy is one of the major highly inducible degradation processes in response to plant developmental and environmental signals. In response to different stimuli, cellular materials, including proteins and organelles, can be sequestered into a double ... ...

Abstract	Autophagy is one of the major highly inducible degradation processes in response to plant developmental and environmental signals. In response to different stimuli, cellular materials, including proteins and organelles, can be sequestered into a double membrane autophagosome structure either selectively or non-selectively. The formation of an autophagosome as well as its delivery into the vacuole involves complex and dynamic membrane processes. The identification and characterization of the conserved autophagy-related (ATG) proteins and their related regulators have greatly advanced our understanding of the molecular mechanism underlying autophagosome biogenesis and function in plant cells. Autophagosome biogenesis is tightly regulated by the coordination of multiple ATG and non-ATG proteins, and selective cargo recruitment. This review updates our current knowledge of autophagosome biogenesis, with special emphasis on the core molecular machinery that drives autophagosome formation, and autophagosome-organelle interactions under abiotic stress conditions.
Language	English
Publishing date	2024-03-27
Publishing country	England
Document type	Journal Article
ZDB-ID	623171-8
ISSN	1532-298X ; 1040-4651
ISSN (online)	1532-298X
ISSN	1040-4651
DOI	10.1093/plcell/koae099
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Article ; Online: A century journey of organelles research in the plant endomembrane system.

Zhuang, Xiaohong / Li, Ruixi / Jiang, Liwen

The Plant cell

2024 Volume 36, Issue 5, Page(s) 1312–1333

Abstract: We are entering an exciting century in the study of the plant organelles in the endomembrane system. Over the past century, especially within the past 50 years, tremendous advancements have been made in the complex plant cell to generate a much clearer ... ...

Abstract	We are entering an exciting century in the study of the plant organelles in the endomembrane system. Over the past century, especially within the past 50 years, tremendous advancements have been made in the complex plant cell to generate a much clearer and informative picture of plant organelles, including the molecular/morphological features, dynamic/spatial behavior, and physiological functions. Importantly, all these discoveries and achievements in the identification and characterization of organelles in the endomembrane system would not have been possible without: (1) the innovations and timely applications of various state-of-art cell biology tools and technologies for organelle biology research; (2) the continuous efforts in developing and characterizing new organelle markers by the plant biology community; and (3) the landmark studies on the identification and characterization of the elusive organelles. While molecular aspects and results for individual organelles have been extensively reviewed, the development of the techniques for organelle research in plant cell biology is less appreciated. As one of the ASPB Centennial Reviews on "organelle biology," here we aim to take a journey across a century of organelle biology research in plants by highlighting the important tools (or landmark technologies) and key scientists that contributed to visualize organelles. We then highlight the landmark studies leading to the identification and characterization of individual organelles in the plant endomembrane systems.
MeSH term(s)	Organelles/metabolism ; Organelles/physiology ; Plants/metabolism ; History, 20th Century ; History, 21st Century ; Plant Cells/physiology ; Intracellular Membranes/metabolism
Language	English
Publishing date	2024-01-16
Publishing country	England
Document type	Journal Article ; Review ; Historical Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	623171-8
ISSN	1532-298X ; 1040-4651
ISSN (online)	1532-298X
ISSN	1040-4651
DOI	10.1093/plcell/koae004
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Article ; Online: A chemometric-assisted method for automatic, rapid and non-targeted detection of multi-pesticides in plant-derived foods by gas chromatography-mass spectrometry.

Su, Guanglin / Xie, Shue / Jiang, Liwen / Du, Guorong / Li, Pao

Food chemistry

2024 Volume 443, Page(s) 138573

Abstract: An automatic, rapid and non-targeted detection method for multi-pesticides in plant-derived foods was developed by gas chromatography-mass spectrometry and chemometrics. In this method, a novel algorithm named moving window iterative target ... ...

Abstract	An automatic, rapid and non-targeted detection method for multi-pesticides in plant-derived foods was developed by gas chromatography-mass spectrometry and chemometrics. In this method, a novel algorithm named moving window iterative target transformation factor analysis was proposed. Although there are challenges of peak overlapping and background interference, the retention time and corrected mass spectra of unknown pesticides can be automatically obtained through iteration calculation in the 'moving window' with reference to the pesticide mass spectral library. One mixed pesticide standard and nine varieties of plant-derived foods were investigated with the proposed method. By contrast, a fast temperature programme was used to shorten detection time compared to the standard temperature programme. For the mixed standard, the mass spectra and retention times of all 39 pesticides were successfully obtained from the overlapping signal. Furthermore, all spiked pesticides were successfully detected in plant-derived foods within 10 min using a fast temperature programme.
MeSH term(s)	Pesticides/analysis ; Gas Chromatography-Mass Spectrometry/methods ; Pesticide Residues/analysis ; Chemometrics ; Mass Spectrometry
Chemical Substances	Pesticides ; Pesticide Residues
Language	English
Publishing date	2024-01-26
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2024.138573
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Article ; Online: Investigating the Structural Evolution and Catalytic Activity of

Chen, Long / Jiang, Li-Wen / Wang, Jian-Jun

Molecules (Basel, Switzerland)

2023 Volume 28, Issue 19

Abstract: Transition metal alloys have emerged as promising electrocatalysts due to their ability to modulate key parameters, such as d-band electron filling, Fermi level energy, and interatomic spacing, thereby influencing their affinity towards reaction ... ...

Abstract	Transition metal alloys have emerged as promising electrocatalysts due to their ability to modulate key parameters, such as d-band electron filling, Fermi level energy, and interatomic spacing, thereby influencing their affinity towards reaction intermediates. However, the structural stability of alloy electrocatalysts during the alkaline hydrogen evolution reaction (HER) remains a subject of debate. In this study, we systematically investigated the structural evolution and catalytic activity of the
Language	English
Publishing date	2023-10-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules28196986
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Article: Effect of bevacizumab administered prior to transarterial chemoembolization on the therapeutic effects of lenvatinib given post-TACE in primary liver cancer patients.

Dang, Zhongfeng / Jiang, Liwen / Huang, Fengping

International journal of clinical pharmacology and therapeutics

2023 Volume 61, Issue 10, Page(s) 423–429

Abstract: Objective: To determine the therapeutic effects of lenvatinib combined with bevacizumab following transarterial chemoembolization (TACE) in patients with primary liver cancer.: Materials and methods: 100 patients with primary liver cancer were ... ...

Abstract	Objective: To determine the therapeutic effects of lenvatinib combined with bevacizumab following transarterial chemoembolization (TACE) in patients with primary liver cancer. Materials and methods: 100 patients with primary liver cancer were recruited in the period from January 2020 to January 2021 and allocated with randomization into a control group (n = 50) and a test (bevacizumab) group (n = 50). The patients in the control group received lenvatinib for 4 weeks following TACE, whereas those in the test group received bevacizumab for 6 weeks prior to TACE and subsequent therapy with lenvatinib for 4 weeks. The serum concentration of interferon-γ (INF-γ), interleukin-10 (IL-10), soluble interleukin-2 receptor (sIL-2R), interleukin-12 (IL-12), superoxide dismutase (SOD), total antioxidant capacity (TAOC), glutathione (GSH), malondialdehyde (MDA), total bilirubin (TBil), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), carbohydrate antigen 242 (CA242), CA724, α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) were determined in both groups at the commencement of treatment in January 2020 and 12 months later and compared with the observed therapeutic effects. Results: The concentrations of CA242, CEA, CA724, and AFP in the bevacizumab group were lower than those in the control group (p < 0.05). The concentration of IL-12 and INF-γ in the bevacizumab group were higher, but the levels of IL-10 and sIL-2R lower than in the control group (p < 0.05). In the bevacizumab group, the level of MDA was lower, whereas the levels of TAOC, SOD, and GSH were higher than those in the control group (p < 0.05). The bevacizumab group also had lower levels of ALT, TBil, and AST and a higher level of ALP than control group (p < 0.05). The response rate based on tumor status (size, progression) in the bevacizumab group was higher than in the control group (p < 0.05). Conclusion: The therapeutic effects of lenvatinib following TACE in primary liver cancer are significantly greater when combined with bevacizumab administered for 6 weeks prior to TACE.
MeSH term(s)	Humans ; Carcinoma, Hepatocellular/drug therapy ; Carcinoma, Hepatocellular/pathology ; Liver Neoplasms/drug therapy ; Liver Neoplasms/pathology ; Bevacizumab/adverse effects ; alpha-Fetoproteins/therapeutic use ; Interleukin-10/therapeutic use ; Carcinoembryonic Antigen/therapeutic use ; Chemoembolization, Therapeutic/adverse effects ; Interleukin-12/therapeutic use ; Superoxide Dismutase ; Treatment Outcome
Chemical Substances	Bevacizumab (2S9ZZM9Q9V) ; alpha-Fetoproteins ; Interleukin-10 (130068-27-8) ; Carcinoembryonic Antigen ; lenvatinib (EE083865G2) ; Interleukin-12 (187348-17-0) ; Superoxide Dismutase (EC 1.15.1.1)
Language	English
Publishing date	2023-08-07
Publishing country	Germany
Document type	Journal Article
ZDB-ID	124384-6
ISSN	0946-1965 ; 0340-0026 ; 0300-9718 ; 0174-4879
ISSN	0946-1965 ; 0340-0026 ; 0300-9718 ; 0174-4879
DOI	10.5414/CP204347
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